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The map label for this gene is lig [H]

Identifier: 212224666

GI number: 212224666

Start: 1390140

End: 1391828

Strand: Reverse

Name: lig [H]

Synonym: TON_1515

Alternate gene names: 212224666

Gene position: 1391828-1390140 (Counterclockwise)

Preceding gene: 212224667

Following gene: 212224664

Centisome position: 75.33

GC content: 54.83

Gene sequence:

>1689_bases
ATGGGAGACATGAAATACACTGAACTCTCTGACCTTTACCGCAGGCTCGAGAAGACTACCCTCAAAACGCTCAAGACTAA
GTTCGTGGCCGACTTTCTGAAGAAAACTCCAGATGAGCTTCTCGAGGTGGTCCCATACCTGATTCTTGGTAAGGTCTTTC
CCGATTGGGACGAGAGGGAGCTTGGTGTTGGTGAGAAGCTCCTCATAAAGGCCGTATCTATGGCAACCGGTGTTCAGGAG
AGGGAAATAGAGAACTCAGTCAAGGACACGGGAGATCTGGGCGAGAGCGTTGCCTTGGCATTAAAGAAAAAGAAGCAGAA
GAGCTTCTTCAGCCAGCCGCTCACGATAAAGCGCGTTTATCAGACTTTCATTAAGATAGCCGAAGCGAGTGGCGAGGGAA
GCCAGGACAGGAAGCTGAAGTACCTCGCAAACATCTTCATGGACGCCCAGCCGGAAGAGGGTAAATACATAGCAAGAACC
GTTCTCGGCATGATGAGAACGGGCGTTGCCGAGGGCATACTTCGCGATGCCATAGCCGAGGCCTTCAAAGTCAAAGCCGA
GCTCGTTGAGCGTGCCTACATGCTCACGAGCGACTTCGGCTACGTTGCAAAGGTGGCGAAGCTCGAAGGCAACGATGGCC
TAGGGAAGGTTCACATTCAGATTGGGAAGCCGATAAGGCCGATGCTCGCCCAGAACGCGGCGAGCGTCAAGGAGGCCCTC
CTCGAGATGGGCGCCGAGGCGGCATTCGAGATAAAGTACGATGGAGCGAGAGTTCAGGTTCACAAGGACGGCGACAGGGT
TGTAATCTACTCCAGGCGGCTGGAGAACGTCACGCGCTCTATCCCCGAGGTCGTTGATGCCATAAAGGCGAGCATAAAGT
CCGAAAAAGCGATAGTTGAGGGTGAGCTTGTCGCTGTGGGTGAGGGTGGAAGGCCAAGACCCTTCCAGTATGTCCTCAGG
CGCTTCAGGAGGAAATACAACATCGAAGAGATGATAGAGAAGATCCCGCTGGAGCTCAACCTTTTCGACGTCCTCTACGT
CGATGGAGAACCCCTGATCGACACCCCCTTCCGCGAGAGGAGAGCTAAGCTTGAAGAAATCGTCGAGGAGGGCGAAAAGC
TCAAGCTTGCCCAGCAACTGGTCACGAAGAAGGTCGAGGAGGCCGAGGAGTTCTACAAGAAGGCCCTTGAACTTGGCCAT
GAGGGTCTCATGGCGAAGAGACTCGATTCCGTCTACGAGCCCGGAAACAGGGGCAAGAAGTGGCTCAAGATAAAGCCCAC
CATGGAAGATCTCGACCTTGTCATAATTGGGGCGGAGTGGGGCGAGGGAAGGAGGGCACACCTGCTCGGCTCCTTCCTGG
TTGCGGCCTATGACCCGCACAGCGGTGAGTTTGTGCCGGTCGGGAAGGTTGGCAGCGGCTTCACCGATGAAGACCTCGTT
GAGTTCACCAAGATGCTCAAGCCCCTCATCATCGGTGGTGAGGGCAAATTCGTGGAGATTGAGCCGAAGGTGGTCATCCA
GGTCACCTACCAGGAGATACAGAAGAGTCCGAAGTACAGGAGCGGGTTTGCGCTGAGGTTCCCGCGCTACGTGGCTCTGA
GAGAGGATAAAAGTCCTGAAGAGGCCGACACGATAGAGCGCATCGCCCAGCTCTACGAGTTCCAGGAGAGGTTCAAGGCT
AAAAAGTGA

Upstream 100 bases:

>100_bases
CCGTTCGACGTCGTCGATGTTTTCATGGGCAAGCCCGAGGTTGGCAGGTGGGTAGGATAGTCTTTTATCCCCTTACTTCC
TATTCTTCTTGGTGGTGAGC

Downstream 100 bases:

>100_bases
ATTCTGAAGCTAGCTCCTCCCTTTCTTCTTCAGGGGCGAGTTTCAGCGCCAGCTCGGCCGCTCTCTTCGCCATTATGGGA
GTTGCGAGCATGAAGGCTGT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MGDMKYTELSDLYRRLEKTTLKTLKTKFVADFLKKTPDELLEVVPYLILGKVFPDWDERELGVGEKLLIKAVSMATGVQE
REIENSVKDTGDLGESVALALKKKKQKSFFSQPLTIKRVYQTFIKIAEASGEGSQDRKLKYLANIFMDAQPEEGKYIART
VLGMMRTGVAEGILRDAIAEAFKVKAELVERAYMLTSDFGYVAKVAKLEGNDGLGKVHIQIGKPIRPMLAQNAASVKEAL
LEMGAEAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTRSIPEVVDAIKASIKSEKAIVEGELVAVGEGGRPRPFQYVLR
RFRRKYNIEEMIEKIPLELNLFDVLYVDGEPLIDTPFRERRAKLEEIVEEGEKLKLAQQLVTKKVEEAEEFYKKALELGH
EGLMAKRLDSVYEPGNRGKKWLKIKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAYDPHSGEFVPVGKVGSGFTDEDLV
EFTKMLKPLIIGGEGKFVEIEPKVVIQVTYQEIQKSPKYRSGFALRFPRYVALREDKSPEEADTIERIAQLYEFQERFKA
KK

Sequences:

>Translated_562_residues
MGDMKYTELSDLYRRLEKTTLKTLKTKFVADFLKKTPDELLEVVPYLILGKVFPDWDERELGVGEKLLIKAVSMATGVQE
REIENSVKDTGDLGESVALALKKKKQKSFFSQPLTIKRVYQTFIKIAEASGEGSQDRKLKYLANIFMDAQPEEGKYIART
VLGMMRTGVAEGILRDAIAEAFKVKAELVERAYMLTSDFGYVAKVAKLEGNDGLGKVHIQIGKPIRPMLAQNAASVKEAL
LEMGAEAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTRSIPEVVDAIKASIKSEKAIVEGELVAVGEGGRPRPFQYVLR
RFRRKYNIEEMIEKIPLELNLFDVLYVDGEPLIDTPFRERRAKLEEIVEEGEKLKLAQQLVTKKVEEAEEFYKKALELGH
EGLMAKRLDSVYEPGNRGKKWLKIKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAYDPHSGEFVPVGKVGSGFTDEDLV
EFTKMLKPLIIGGEGKFVEIEPKVVIQVTYQEIQKSPKYRSGFALRFPRYVALREDKSPEEADTIERIAQLYEFQERFKA
KK
>Mature_561_residues
GDMKYTELSDLYRRLEKTTLKTLKTKFVADFLKKTPDELLEVVPYLILGKVFPDWDERELGVGEKLLIKAVSMATGVQER
EIENSVKDTGDLGESVALALKKKKQKSFFSQPLTIKRVYQTFIKIAEASGEGSQDRKLKYLANIFMDAQPEEGKYIARTV
LGMMRTGVAEGILRDAIAEAFKVKAELVERAYMLTSDFGYVAKVAKLEGNDGLGKVHIQIGKPIRPMLAQNAASVKEALL
EMGAEAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTRSIPEVVDAIKASIKSEKAIVEGELVAVGEGGRPRPFQYVLRR
FRRKYNIEEMIEKIPLELNLFDVLYVDGEPLIDTPFRERRAKLEEIVEEGEKLKLAQQLVTKKVEEAEEFYKKALELGHE
GLMAKRLDSVYEPGNRGKKWLKIKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAYDPHSGEFVPVGKVGSGFTDEDLVE
FTKMLKPLIIGGEGKFVEIEPKVVIQVTYQEIQKSPKYRSGFALRFPRYVALREDKSPEEADTIERIAQLYEFQERFKAK
K

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=626, Percent_Identity=30.9904153354633, Blast_Score=246, Evalue=5e-65,
Organism=Homo sapiens, GI46255052, Length=500, Percent_Identity=28.4, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI23199993, Length=500, Percent_Identity=28.4, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI148539894, Length=500, Percent_Identity=28.4, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI73747829, Length=577, Percent_Identity=25.6499133448873, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI73747844, Length=577, Percent_Identity=25.6499133448873, Blast_Score=151, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71985269, Length=587, Percent_Identity=31.0051107325383, Blast_Score=268, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI71985278, Length=158, Percent_Identity=30.379746835443, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71981465, Length=351, Percent_Identity=25.0712250712251, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320038, Length=616, Percent_Identity=29.8701298701299, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6324578, Length=454, Percent_Identity=23.3480176211454, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24762424, Length=616, Percent_Identity=30.0324675324675, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI85815841, Length=542, Percent_Identity=26.1992619926199, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24641829, Length=542, Percent_Identity=24.9077490774908, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 63656; Mature: 63524

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDMKYTELSDLYRRLEKTTLKTLKTKFVADFLKKTPDELLEVVPYLILGKVFPDWDERE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
LGVGEKLLIKAVSMATGVQEREIENSVKDTGDLGESVALALKKKKQKSFFSQPLTIKRVY
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QTFIKIAEASGEGSQDRKLKYLANIFMDAQPEEGKYIARTVLGMMRTGVAEGILRDAIAE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFKVKAELVERAYMLTSDFGYVAKVAKLEGNDGLGKVHIQIGKPIRPMLAQNAASVKEAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCHHHHHHHH
LEMGAEAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTRSIPEVVDAIKASIKSEKAIVE
HHCCCCEEEEEEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHEEC
GELVAVGEGGRPRPFQYVLRRFRRKYNIEEMIEKIPLELNLFDVLYVDGEPLIDTPFRER
CCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHH
RAKLEEIVEEGEKLKLAQQLVTKKVEEAEEFYKKALELGHEGLMAKRLDSVYEPGNRGKK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCE
WLKIKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAYDPHSGEFVPVGKVGSGFTDEDLV
EEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCCEEECCCCCCCCCHHHHH
EFTKMLKPLIIGGEGKFVEIEPKVVIQVTYQEIQKSPKYRSGFALRFPRYVALREDKSPE
HHHHHHHHEEECCCCCEEEECCEEEEEEEHHHHHCCCCCCCCCEEECCCEEEECCCCCCC
EADTIERIAQLYEFQERFKAKK
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GDMKYTELSDLYRRLEKTTLKTLKTKFVADFLKKTPDELLEVVPYLILGKVFPDWDERE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
LGVGEKLLIKAVSMATGVQEREIENSVKDTGDLGESVALALKKKKQKSFFSQPLTIKRVY
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QTFIKIAEASGEGSQDRKLKYLANIFMDAQPEEGKYIARTVLGMMRTGVAEGILRDAIAE
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AFKVKAELVERAYMLTSDFGYVAKVAKLEGNDGLGKVHIQIGKPIRPMLAQNAASVKEAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHCCHHHHHHHH
LEMGAEAAFEIKYDGARVQVHKDGDRVVIYSRRLENVTRSIPEVVDAIKASIKSEKAIVE
HHCCCCEEEEEEECCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHEEC
GELVAVGEGGRPRPFQYVLRRFRRKYNIEEMIEKIPLELNLFDVLYVDGEPLIDTPFRER
CCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHH
RAKLEEIVEEGEKLKLAQQLVTKKVEEAEEFYKKALELGHEGLMAKRLDSVYEPGNRGKK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCE
WLKIKPTMEDLDLVIIGAEWGEGRRAHLLGSFLVAAYDPHSGEFVPVGKVGSGFTDEDLV
EEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHEECCCCCCEEECCCCCCCCCHHHHH
EFTKMLKPLIIGGEGKFVEIEPKVVIQVTYQEIQKSPKYRSGFALRFPRYVALREDKSPE
HHHHHHHHEEECCCCCEEEECCEEEEEEEHHHHHCCCCCCCCCEEECCCEEEECCCCCCC
EADTIERIAQLYEFQERFKAKK
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA