Click here to switch to the map view.

The map label for this gene is tfb [H]

Identifier: 212224249

GI number: 212224249

Start: 1020872

End: 1021771

Strand: Reverse

Name: tfb [H]

Synonym: TON_1100

Alternate gene names: 212224249

Gene position: 1021771-1020872 (Counterclockwise)

Preceding gene: 212224250

Following gene: 212224246

Centisome position: 55.3

GC content: 56.33

Gene sequence:

>900_bases
GTGAGCAAGCGTAGGGTCTGCCCGGTATGTGGCTCAACGGAGTTCATCTACGACCCGGGTAGGGGAGAGGTAGTCTGTAA
GGTTTGCGGTTATGTTATAGAGGAGAACGTCATAGATATGGGTCCGGAATGGCGCGCTTTTGACGCTTCTCAAAGAGAGA
AGAGGGCCCGCGTTGGTGCCCCCGAGAGCATTCTCCTTCACGATAAGGGTCTCTCAACTGATATAGGCATCGACAGGAAC
CTTTCTGGATTGATGCGCGAGAAGATGTACCGTCTGAGGAAGTGGCAATCCCGTCTGAGGGTTAGCGATGCCGCCGAGCG
TAACTTGGCATTCGCCCTGAGCGAGCTTGACAGGATAGCGAGCCAGCTCAAGCTTCCGAGACACGTTGAAGAAGAGGCTG
CTCGTCTCTACCGTGAGGCCGTCAGGAAGGGTCTCATAAGGGGCCGCTCCATTGAGAGCGTCATAGCGGCATGTGTTTAC
GCTGCGTGCAGGCTCCTCAAAGTTCCGAGGACGCTCGACGAGATAGCGGACATATCGCGCGTCGACAAGAAGGAGATAGG
CAGGAGCTTCCGCTTCATCGCGAGGAACCTCAACCTTACGCCAAAGAAGCTCTTCGTAAAGCCAACGGACTACGTCAACA
AGTTTGCCGATGAGCTCGGCCTGAGCGAGAAGGTCCGGAGAAGGGCCATTGCGATTCTCGAGGAGGCCTACGAGAAAGGC
CTCACGAGCGGAAAGAGCCCGGCCGGCCTTGTTGCGGCGGCCCTGTATATAGCGGGCCTGCTTGAGGACGAGAAGAGGAC
GCAGCGTGAGGTTGCAGAGGTTGCGCGCGTCACAGAGGTAACGGTAAGGAACAGATACAAAGAGCTGGTGGAGAAGCTGA
ACCTCAAGATTCCGGTTTGA

Upstream 100 bases:

>100_bases
AACGTGAAGACCTCGCCTGGATTCTTAGGTATGTGGCGAAGAAAAAGGGCGAAGGTTTATAAGGGCTTTATAAAATAGCC
GGCCGGTTGGGGGTGTTGGA

Downstream 100 bases:

>100_bases
AGGAAAGAGAGACTACCTCCGCCCAAACCAGCTCTCGAGCGTCGACTGCTTTCCTGCCTTTACGGCTTTCTTCAGCCTTT
CAAGGCCATTCTTCACTCTC

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB 1 [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSKRRVCPVCGSTEFIYDPGRGEVVCKVCGYVIEENVIDMGPEWRAFDASQREKRARVGAPESILLHDKGLSTDIGIDRN
LSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIASQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVY
AACRLLKVPRTLDEIADISRVDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIAILEEAYEKG
LTSGKSPAGLVAAALYIAGLLEDEKRTQREVAEVARVTEVTVRNRYKELVEKLNLKIPV

Sequences:

>Translated_299_residues
MSKRRVCPVCGSTEFIYDPGRGEVVCKVCGYVIEENVIDMGPEWRAFDASQREKRARVGAPESILLHDKGLSTDIGIDRN
LSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIASQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVY
AACRLLKVPRTLDEIADISRVDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIAILEEAYEKG
LTSGKSPAGLVAAALYIAGLLEDEKRTQREVAEVARVTEVTVRNRYKELVEKLNLKIPV
>Mature_298_residues
SKRRVCPVCGSTEFIYDPGRGEVVCKVCGYVIEENVIDMGPEWRAFDASQREKRARVGAPESILLHDKGLSTDIGIDRNL
SGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIASQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVYA
ACRLLKVPRTLDEIADISRVDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIAILEEAYEKGL
TSGKSPAGLVAAALYIAGLLEDEKRTQREVAEVARVTEVTVRNRYKELVEKLNLKIPV

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=285, Percent_Identity=34.0350877192982, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI22035556, Length=288, Percent_Identity=30.5555555555556, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17564892, Length=301, Percent_Identity=33.5548172757475, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531397, Length=291, Percent_Identity=24.7422680412371, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325343, Length=304, Percent_Identity=28.9473684210526, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321685, Length=289, Percent_Identity=24.9134948096886, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17136758, Length=276, Percent_Identity=34.0579710144928, Blast_Score=167, Evalue=6e-42,
Organism=Drosophila melanogaster, GI281361961, Length=294, Percent_Identity=27.5510204081633, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24647658, Length=294, Percent_Identity=27.5510204081633, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 33890; Mature: 33759

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS51134 ZF_TFIIB ; PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRRVCPVCGSTEFIYDPGRGEVVCKVCGYVIEENVIDMGPEWRAFDASQREKRARVGA
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
PESILLHDKGLSTDIGIDRNLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIA
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIADISR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIAILEEAYEKG
HHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LTSGKSPAGLVAAALYIAGLLEDEKRTQREVAEVARVTEVTVRNRYKELVEKLNLKIPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKRRVCPVCGSTEFIYDPGRGEVVCKVCGYVIEENVIDMGPEWRAFDASQREKRARVGA
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCC
PESILLHDKGLSTDIGIDRNLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIA
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVIAACVYAACRLLKVPRTLDEIADISR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKKEIGRSFRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIAILEEAYEKG
HHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LTSGKSPAGLVAAALYIAGLLEDEKRTQREVAEVARVTEVTVRNRYKELVEKLNLKIPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA