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The map label for this gene is thiI [H]

Identifier: 212224157

GI number: 212224157

Start: 931496

End: 932647

Strand: Reverse

Name: thiI [H]

Synonym: TON_1008

Alternate gene names: 212224157

Gene position: 932647-931496 (Counterclockwise)

Preceding gene: 212224158

Following gene: 212224156

Centisome position: 50.48

GC content: 52.6

Gene sequence:

>1152_bases
ATGCTCATGTTCAACGTCGTCATAGTCCGCTACGGTGAGATAGGCACGAAGTCGAGGCAGACGAGGAGATGGTTCGAGAA
CATCCTCATGAACAACATCCGTGAGGCCCTGGTGAGTGAGGAAGTCGAGTTCAAGAAGGTAGAAGCAAAGCACGGAAGGG
TTCTTGTAAAGACAAACAGGGCTGAAGAGGCTGTTGAGGTTCTTAAGCGGGTCTTCGGCATAGTCTCGCTTTCCCCGGCG
ATGGAGATCGACGCAGAAATGGAGAAGATCAACAGGATGGCCCTCAAGCTATTCCGCAGAAAGAAGAGAGAGCTTAGATT
AGAAAAACCTCGCTTTAGGGTAACCGCGAGGCGCATAACCAAGGAGTTCCCCCTGAAGAGTCCGGAGATCCAGGCGAAGG
TCGGCGAGTACATCCTCGAGAACGAAGAGAGCGAGGTGAATCTCCACGAGTATGACATAGAAGTTGGCGTTGAGCTAATG
GAGGGCAAGGCCTACATCTTCGTGGACAAGGTCAAAGCCTGGGGTGGCCTTCCCATCGGCACTCAGGGCAAGGTCGTGGC
TCTGCTCAGCGGCGGCATTGATTCACCGGTTGCCGCTTTCCTCATGATGAAGCGCGGTGTAGAGGTAATCCCGGTGCACA
TCTACATGGGCGAGAAGACCCTCGAAAAGGTCAGGAAGATATGGAACCAGCTTAGGAAGTACGGCTATGGTGGAAAGGGC
GAGCTGATTGTGGTTAAACCCAAAGAGCGGGAAAGAATTCTCCAGAAACTCAAGGAGCTTAAAAAAGAGAACTACACCTG
TGTCTTCTGCAAGTTCATGATGGTTCGCCACGCCGATAGAATCGCGAAGGACTTTGGGGCAAAGGGCATCGTGATGGGTG
ACTCCCTCGGCCAGGTGGCCTCTCAAACCCTGGAGAACATGTACATCGTCAGCCGGGCGAGCGATCTGCCGATTTACCGC
CCGCTCATAGGCATGGACAAGGAGGAAATCGTCAAAATAGCAAAGGAGATAGGCACCTTTGAGCTGTCCACTCTCCCTGA
TGACGAGATTCCCTTTATACCGAGGCATCCAGTAATAAGGGGTTCCTGGGAGGAGTTCAGAAAGCTCTACAAGGCCGTCT
TCGGCGAAGAACCGAAAAATAGGGCATGTTGA

Upstream 100 bases:

>100_bases
AGCTGATACTCCTTTCCTCATATCTCATCTCGATCGTCCTTTGTGTCGTCATTCAAAAGTACGTCGAGATTGTGATTAAG
TTCCCTCATATGGTGAGGTG

Downstream 100 bases:

>100_bases
GGTGATGTTATGGAGTCGACAAAACTCCCGGCATACATCTCCCTCCTCCTACCACTCGTATTTCTTATTGGGCTAATCGT
TGTCATTTACCAGAATCCTT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MLMFNVVIVRYGEIGTKSRQTRRWFENILMNNIREALVSEEVEFKKVEAKHGRVLVKTNRAEEAVEVLKRVFGIVSLSPA
MEIDAEMEKINRMALKLFRRKKRELRLEKPRFRVTARRITKEFPLKSPEIQAKVGEYILENEESEVNLHEYDIEVGVELM
EGKAYIFVDKVKAWGGLPIGTQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLRKYGYGGKG
ELIVVKPKERERILQKLKELKKENYTCVFCKFMMVRHADRIAKDFGAKGIVMGDSLGQVASQTLENMYIVSRASDLPIYR
PLIGMDKEEIVKIAKEIGTFELSTLPDDEIPFIPRHPVIRGSWEEFRKLYKAVFGEEPKNRAC

Sequences:

>Translated_383_residues
MLMFNVVIVRYGEIGTKSRQTRRWFENILMNNIREALVSEEVEFKKVEAKHGRVLVKTNRAEEAVEVLKRVFGIVSLSPA
MEIDAEMEKINRMALKLFRRKKRELRLEKPRFRVTARRITKEFPLKSPEIQAKVGEYILENEESEVNLHEYDIEVGVELM
EGKAYIFVDKVKAWGGLPIGTQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLRKYGYGGKG
ELIVVKPKERERILQKLKELKKENYTCVFCKFMMVRHADRIAKDFGAKGIVMGDSLGQVASQTLENMYIVSRASDLPIYR
PLIGMDKEEIVKIAKEIGTFELSTLPDDEIPFIPRHPVIRGSWEEFRKLYKAVFGEEPKNRAC
>Mature_383_residues
MLMFNVVIVRYGEIGTKSRQTRRWFENILMNNIREALVSEEVEFKKVEAKHGRVLVKTNRAEEAVEVLKRVFGIVSLSPA
MEIDAEMEKINRMALKLFRRKKRELRLEKPRFRVTARRITKEFPLKSPEIQAKVGEYILENEESEVNLHEYDIEVGVELM
EGKAYIFVDKVKAWGGLPIGTQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLRKYGYGGKG
ELIVVKPKERERILQKLKELKKENYTCVFCKFMMVRHADRIAKDFGAKGIVMGDSLGQVASQTLENMYIVSRASDLPIYR
PLIGMDKEEIVKIAKEIGTFELSTLPDDEIPFIPRHPVIRGSWEEFRKLYKAVFGEEPKNRAC

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=361, Percent_Identity=29.0858725761773, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 44138; Mature: 44138

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMFNVVIVRYGEIGTKSRQTRRWFENILMNNIREALVSEEVEFKKVEAKHGRVLVKTNR
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECC
AEEAVEVLKRVFGIVSLSPAMEIDAEMEKINRMALKLFRRKKRELRLEKPRFRVTARRIT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KEFPLKSPEIQAKVGEYILENEESEVNLHEYDIEVGVELMEGKAYIFVDKVKAWGGLPIG
HHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHCCCEEECCCEEEEEEEEHHCCCCCCC
TQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLRKYGYGGKG
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
ELIVVKPKERERILQKLKELKKENYTCVFCKFMMVRHADRIAKDFGAKGIVMGDSLGQVA
CEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
SQTLENMYIVSRASDLPIYRPLIGMDKEEIVKIAKEIGTFELSTLPDDEIPFIPRHPVIR
HHHHHHHEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCC
GSWEEFRKLYKAVFGEEPKNRAC
CCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLMFNVVIVRYGEIGTKSRQTRRWFENILMNNIREALVSEEVEFKKVEAKHGRVLVKTNR
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECC
AEEAVEVLKRVFGIVSLSPAMEIDAEMEKINRMALKLFRRKKRELRLEKPRFRVTARRIT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KEFPLKSPEIQAKVGEYILENEESEVNLHEYDIEVGVELMEGKAYIFVDKVKAWGGLPIG
HHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHCCCEEECCCEEEEEEEEHHCCCCCCC
TQGKVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMGEKTLEKVRKIWNQLRKYGYGGKG
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHHHHHHHHCCCCCCC
ELIVVKPKERERILQKLKELKKENYTCVFCKFMMVRHADRIAKDFGAKGIVMGDSLGQVA
CEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHH
SQTLENMYIVSRASDLPIYRPLIGMDKEEIVKIAKEIGTFELSTLPDDEIPFIPRHPVIR
HHHHHHHEEEECCCCCCCCCHHHCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCC
GSWEEFRKLYKAVFGEEPKNRAC
CCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA