Click here to switch to the map view.

The map label for this gene is lysK [H]

Identifier: 212224087

GI number: 212224087

Start: 864851

End: 865903

Strand: Reverse

Name: lysK [H]

Synonym: TON_0938

Alternate gene names: 212224087

Gene position: 865903-864851 (Counterclockwise)

Preceding gene: 212224092

Following gene: 212224084

Centisome position: 46.87

GC content: 52.52

Gene sequence:

>1053_bases
ATGAAAACTGAGAGAGCAAAGGAAATCCTTCTTAACCTCCTCAAAATTCCGTCCCCCTCAGGGAGCGAGGATCGTATAGC
CCTTCACATCATGGAGTTCCTCCACAAGCTGGACTACGACGTCCACATTGAGAGTGACGGCGAGATAATCGACCTCGTCG
TCAATCCCGACGCTGAGCTCTTCTACGAGGTCCATATGGACACGATACCGATGAGGGCCGAGCCCTTTGTAAGGGGTAAC
ATCGTTTACGGAACCGGCGCGAGCGACATAAAGGGCGGCATAGCTGCGATTCTGCTTATGCTCGAAGAACTCAAGAAAGA
GGATAAGGATCTCAACGTCGGCATTGTCTTCGTCAGCGACGAAGAGCACGGCGGAAGAGGCTCGGCACTCTTTATGGAGC
GGTACAGGCCCAAGATGGCCGTTGTGCTTGAGCCCACTGATCTAGAGGTCCACATAGCCCACGCAGGCAACATAGAGGCC
TACTTTGAGGTCGACGGCAAGGAGGCCCACGGAGCCTGCCCTGAGAGTGGAATCAACGCCATAGATCAGACCTTCAAGAT
GCTTGAGGAACTCAAGAAGCTCGAACCTTTCAACGCGAAGGGCAAGTATTTCGACGCCCACATCGGCCTTCAGGAGCTAG
TCTGCGACAACCCATACTACCTTATCCCTGCCCTCTGCCGTGGTCGTGTGGAGGCTCGCCTTCTGCCAGATCAGGAAGTC
GAGGACATCCTTGACCTTATGGAGCCAATATTTGAGGAGTACACCCTCAAGTACGAGTACACGGAGATATGGGACGGTTA
CGAGCTTGAACCCGATGAGGAGATAGTCGTTCTCGCTAAAAAGGCTATGGAAGCAACAGAAATAGACGAGTTCGGTGGCA
TGAGAAGCTGGACGGACGCGATAAACTTCGTGTACAACGGAACGAGAACGATAGTCTTCGGCCCAGGCAACCTCGACATC
TCACACACAAAGAACGAGCACATCGATGTGAGGGACGTGGTTACAGCAAGTGAGTTTTTGAAGGCCTTGAATGAGATTTT
TGGAAAGAGCTGA

Upstream 100 bases:

>100_bases
GAGCGAGTCTCGTCAGGAGGGATGAATCTCTTCATCGCCCCTTCAATCTATGCGGGGGAGGTTTATAAACCCAACCACAG
AGCTTTATTAGGTGACCCAA

Downstream 100 bases:

>100_bases
GGTTCTCCCTTCTTTTTACTTTCCCTTCGTATTATGGCCTTTATATAACTGGTCCACCAACAGATAATTTGTGGAACCCA
TTATTCCTCAGACTTCTTCT

Product: acetylornithine deacetylase

Products: Acetate; Ornithine [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKTERAKEILLNLLKIPSPSGSEDRIALHIMEFLHKLDYDVHIESDGEIIDLVVNPDAELFYEVHMDTIPMRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEELKKEDKDLNVGIVFVSDEEHGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIDQTFKMLEELKKLEPFNAKGKYFDAHIGLQELVCDNPYYLIPALCRGRVEARLLPDQEV
EDILDLMEPIFEEYTLKYEYTEIWDGYELEPDEEIVVLAKKAMEATEIDEFGGMRSWTDAINFVYNGTRTIVFGPGNLDI
SHTKNEHIDVRDVVTASEFLKALNEIFGKS

Sequences:

>Translated_350_residues
MKTERAKEILLNLLKIPSPSGSEDRIALHIMEFLHKLDYDVHIESDGEIIDLVVNPDAELFYEVHMDTIPMRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEELKKEDKDLNVGIVFVSDEEHGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIDQTFKMLEELKKLEPFNAKGKYFDAHIGLQELVCDNPYYLIPALCRGRVEARLLPDQEV
EDILDLMEPIFEEYTLKYEYTEIWDGYELEPDEEIVVLAKKAMEATEIDEFGGMRSWTDAINFVYNGTRTIVFGPGNLDI
SHTKNEHIDVRDVVTASEFLKALNEIFGKS
>Mature_350_residues
MKTERAKEILLNLLKIPSPSGSEDRIALHIMEFLHKLDYDVHIESDGEIIDLVVNPDAELFYEVHMDTIPMRAEPFVRGN
IVYGTGASDIKGGIAAILLMLEELKKEDKDLNVGIVFVSDEEHGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEA
YFEVDGKEAHGACPESGINAIDQTFKMLEELKKLEPFNAKGKYFDAHIGLQELVCDNPYYLIPALCRGRVEARLLPDQEV
EDILDLMEPIFEEYTLKYEYTEIWDGYELEPDEEIVVLAKKAMEATEIDEFGGMRSWTDAINFVYNGTRTIVFGPGNLDI
SHTKNEHIDVRDVVTASEFLKALNEIFGKS

Specific function: Displays A Broad Specificity And Can Also Deacylate Substrates Such As Acetylarginine, Acetylhistidine Or Acetylglutamate Semialdehyde. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. LysK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=306, Percent_Identity=26.4705882352941, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789236, Length=379, Percent_Identity=25.3298153034301, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010175
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.16 [C]

Molecular weight: Translated: 39543; Mature: 39543

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTERAKEILLNLLKIPSPSGSEDRIALHIMEFLHKLDYDVHIESDGEIIDLVVNPDAEL
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHE
FYEVHMDTIPMRAEPFVRGNIVYGTGASDIKGGIAAILLMLEELKKEDKDLNVGIVFVSD
EEEEECCCCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
EEHGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEAYFEVDGKEAHGACPESGINA
CCCCCCCCEEHHHHCCCCEEEEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHH
IDQTFKMLEELKKLEPFNAKGKYFDAHIGLQELVCDNPYYLIPALCRGRVEARLLPDQEV
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEHHHHCCCCEEEECCCHHH
EDILDLMEPIFEEYTLKYEYTEIWDGYELEPDEEIVVLAKKAMEATEIDEFGGMRSWTDA
HHHHHHHHHHHHHHEEEEEEHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHH
INFVYNGTRTIVFGPGNLDISHTKNEHIDVRDVVTASEFLKALNEIFGKS
HHHEECCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTERAKEILLNLLKIPSPSGSEDRIALHIMEFLHKLDYDVHIESDGEIIDLVVNPDAEL
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCHHE
FYEVHMDTIPMRAEPFVRGNIVYGTGASDIKGGIAAILLMLEELKKEDKDLNVGIVFVSD
EEEEECCCCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
EEHGGRGSALFMERYRPKMAVVLEPTDLEVHIAHAGNIEAYFEVDGKEAHGACPESGINA
CCCCCCCCEEHHHHCCCCEEEEECCCCEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHH
IDQTFKMLEELKKLEPFNAKGKYFDAHIGLQELVCDNPYYLIPALCRGRVEARLLPDQEV
HHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEHHHHCCCCEEEECCCHHH
EDILDLMEPIFEEYTLKYEYTEIWDGYELEPDEEIVVLAKKAMEATEIDEFGGMRSWTDA
HHHHHHHHHHHHHHEEEEEEHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHH
INFVYNGTRTIVFGPGNLDISHTKNEHIDVRDVVTASEFLKALNEIFGKS
HHHEECCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Glutathione. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N2-Acetyl-L-ornithine; H2O [C]

Specific reaction: N2-Acetyl-L-ornithine + H2O --> Acetate + Ornithine [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]