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The map label for this gene is purA

Identifier: 212224060

GI number: 212224060

Start: 841189

End: 842208

Strand: Reverse

Name: purA

Synonym: TON_0911

Alternate gene names: 212224060

Gene position: 842208-841189 (Counterclockwise)

Preceding gene: 212224062

Following gene: 212224051

Centisome position: 45.58

GC content: 54.31

Gene sequence:

>1020_bases
ATGCCGAGCTACATAGTTGTTGGTGGTCAATGGGGAGACGAGGGTAAGGGGTCTCTTATAGCCTACCTTGCCTTGAAGGA
TGAGCCTCAAATCATAGCACGCGGCGGCGTTGGGACAAACGCGGGCCACAGCGTCTTCATCAACGGTAGGAAATACGCAG
TCAGACAGCTCCCAACGGGCTTTATGCAGACTAAAGCTAGGCTTCTTGTTGGGGCTGGAGTTCTTGTTGATCCAGGGGTC
TTTTTCCATGAGCTTGAACACCTGAAGGAGTTCAATGTTGCCGAAAGAGTTGGCATTGATTACCGTTGCGCCATTATAGA
GCCAAGACACAAGGAGCGCGATAGAGCGAGCAGCTATCTGCACGACAAAATCGGAACCACGGGTTCCGGCTGTGGGCCGG
CAAACGCTGATAGGGTCATGCGTATGGCAAAACAGGCGAAGGACATCAAGGAGCTGGAGCCATATCTCACGGACGTTGCT
CAAGAAATTAACGACGCGCTCGATGAAGGCAAGCTGGTTCTCATCGAGGGCACACAGGGCTTTGGACTGAGCCTCTACTA
CGGTACTTACCCCTACGTGACCTCGAAGGATACAACCGCTTCAGCCATAGCCAGCGACGTCGGAATCGGGCCCACGAGGG
TCGACGATGTTATAGTCGTCTTCAAAAGCTTTCCGACGCGCGTTGGAGAGGGTCCGTTCCCGACGGAGATGAGTGCTGAG
GAAGCTGAGAGGCTCGGTCTCGTCGAGTACGGCACTGTCACAGGAAGGCGCAGGCGCGTCGGCTGGTTCGACTTCGAGTT
CGCGCGCTATTCGGCGAGGCTCAACGGGGCGACGATGCTGGCTATAACAATGCTCGACAAGTACGACAAAGGGGCCTTCG
GCGTTACCGACTACGACAAGCTGCCAAGGAAGGCCAAGGAGTTTATAGAAGAAATAGAGGAAAGGGTTGGTATTCCGGTC
GGCATAATCAAGACTGGTCCTGAGCTTGAGCACGTGATTGATAGGAGGGATACTCTCTAA

Upstream 100 bases:

>100_bases
TTTAACCTTTTGCTGTCTCTTTCCGTGGGATTTTGACAAACTTTTGTCTGATTAGAAACTGTTATAAGGCCCCTATTGAC
TTAAACTTGGGTAAGGAGCC

Downstream 100 bases:

>100_bases
TTCTCTTTTCTTCCTCCCGCGTGGGCCACTAACCACTCAACTATCGTTCTGTGAAACTCATTCCCCCACTCAGGATCCTC
GAATATCTCGTGGTAGGCCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPSYIVVGGQWGDEGKGSLIAYLALKDEPQIIARGGVGTNAGHSVFINGRKYAVRQLPTGFMQTKARLLVGAGVLVDPGV
FFHELEHLKEFNVAERVGIDYRCAIIEPRHKERDRASSYLHDKIGTTGSGCGPANADRVMRMAKQAKDIKELEPYLTDVA
QEINDALDEGKLVLIEGTQGFGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGEGPFPTEMSAE
EAERLGLVEYGTVTGRRRRVGWFDFEFARYSARLNGATMLAITMLDKYDKGAFGVTDYDKLPRKAKEFIEEIEERVGIPV
GIIKTGPELEHVIDRRDTL

Sequences:

>Translated_339_residues
MPSYIVVGGQWGDEGKGSLIAYLALKDEPQIIARGGVGTNAGHSVFINGRKYAVRQLPTGFMQTKARLLVGAGVLVDPGV
FFHELEHLKEFNVAERVGIDYRCAIIEPRHKERDRASSYLHDKIGTTGSGCGPANADRVMRMAKQAKDIKELEPYLTDVA
QEINDALDEGKLVLIEGTQGFGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGEGPFPTEMSAE
EAERLGLVEYGTVTGRRRRVGWFDFEFARYSARLNGATMLAITMLDKYDKGAFGVTDYDKLPRKAKEFIEEIEERVGIPV
GIIKTGPELEHVIDRRDTL
>Mature_338_residues
PSYIVVGGQWGDEGKGSLIAYLALKDEPQIIARGGVGTNAGHSVFINGRKYAVRQLPTGFMQTKARLLVGAGVLVDPGVF
FHELEHLKEFNVAERVGIDYRCAIIEPRHKERDRASSYLHDKIGTTGSGCGPANADRVMRMAKQAKDIKELEPYLTDVAQ
EINDALDEGKLVLIEGTQGFGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGEGPFPTEMSAEE
AERLGLVEYGTVTGRRRRVGWFDFEFARYSARLNGATMLAITMLDKYDKGAFGVTDYDKLPRKAKEFIEEIEERVGIPVG
IIKTGPELEHVIDRRDTL

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=28.3018867924528, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI34577063, Length=338, Percent_Identity=30.1775147928994, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=26.7857142857143, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=31.3817330210773, Blast_Score=197, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI25146555, Length=424, Percent_Identity=30.4245283018868, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25146553, Length=424, Percent_Identity=30.4245283018868, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=30.0699300699301, Blast_Score=196, Evalue=3e-51,
Organism=Drosophila melanogaster, GI21356033, Length=337, Percent_Identity=29.9703264094956, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_THEON (B6YWD6)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307296.1
- GeneID:   7017214
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0911
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK04293
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 37360; Mature: 37229

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 43-43 BINDING 127-127 BINDING 141-141 BINDING 179-179 BINDING 194-194 BINDING 256-256 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYIVVGGQWGDEGKGSLIAYLALKDEPQIIARGGVGTNAGHSVFINGRKYAVRQLPTG
CCCEEEEECCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCHHHHHCCCH
FMQTKARLLVGAGVLVDPGVFFHELEHLKEFNVAERVGIDYRCAIIEPRHKERDRASSYL
HHHHHHHHHEECCEEECCCHHHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHHH
HDKIGTTGSGCGPANADRVMRMAKQAKDIKELEPYLTDVAQEINDALDEGKLVLIEGTQG
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGEGPFPTEMSAE
CCEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHHEEEEHHCCCCCCCCCCCCCCCHH
EAERLGLVEYGTVTGRRRRVGWFDFEFARYSARLNGATMLAITMLDKYDKGAFGVTDYDK
HHHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCHHH
LPRKAKEFIEEIEERVGIPVGIIKTGPELEHVIDRRDTL
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure 
PSYIVVGGQWGDEGKGSLIAYLALKDEPQIIARGGVGTNAGHSVFINGRKYAVRQLPTG
CCEEEEECCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCHHHHHCCCH
FMQTKARLLVGAGVLVDPGVFFHELEHLKEFNVAERVGIDYRCAIIEPRHKERDRASSYL
HHHHHHHHHEECCEEECCCHHHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHHH
HDKIGTTGSGCGPANADRVMRMAKQAKDIKELEPYLTDVAQEINDALDEGKLVLIEGTQG
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
FGLSLYYGTYPYVTSKDTTASAIASDVGIGPTRVDDVIVVFKSFPTRVGEGPFPTEMSAE
CCEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHHEEEEHHCCCCCCCCCCCCCCCHH
EAERLGLVEYGTVTGRRRRVGWFDFEFARYSARLNGATMLAITMLDKYDKGAFGVTDYDK
HHHHCCCEEECCCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCHHH
LPRKAKEFIEEIEERVGIPVGIIKTGPELEHVIDRRDTL
HHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA