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The map label for this gene is birA [H]

Identifier: 212224051

GI number: 212224051

Start: 832602

End: 833624

Strand: Reverse

Name: birA [H]

Synonym: TON_0902

Alternate gene names: 212224051

Gene position: 833624-832602 (Counterclockwise)

Preceding gene: 212224060

Following gene: 212224050

Centisome position: 45.12

GC content: 51.91

Gene sequence:

>1023_bases
ATGACGCCGTCAACACCGGCGCGCATAACCTTTCTAATGATTATCTTCGGGTTCACGTGCTCCCAGTGCTCCTCAAAATC
TGTCGGTCCGTGCTCGAAGCCGTGGTCCATCGCAAATATGAGCGTCCTTCCGTTTCTGCGGAAGAACCTTTTCATTCTTC
TTCTAATTCCAATACTCTGGTATGCATCCATACTAATCACCTTGGGTGATATATGGTTTGGGGGATTTAAGGTTTTCTTT
GATGGAAAGTTGTTTAAGTCCAGGATGAGAAGTTCGGCCGGTGGTTTAATGTCGGGACTGGGGAGTGAAATTATCGGCCG
TAAAATAATTCACTTCCACGAAGTGGATTCAACCAACGAGTACGCCAAGCTTATCGCTCTGGACGAGCGTGAGGGGACGG
TGGTGGTGGCGGACAGACAGACCGCCGGAAAGGGGCGAAAAGGTCGCACCTGGGCCTCCCCGGAGGGAGGCCTCTGGATG
AGTGTCATCCTGAAGCCTAAGGCTGAGCCCAAGCACCTTTCCAAGCTCGTCTTCATCGGAGCCTTGGCCGTTGTCGATAC
TCTCTGGAAATTTGGAATCGAAGCGGGTATAAAATGGCCCAACGACGTCTGGGTAAACGGGAGAAAAATCTGCGGGGTTC
TTACTGAGGGAAGGATCGGGGAGTTCGTTATTCTCGGGATAGGCCTCAACGTGAACAACGACGCCCCTGAGGAGCTCAGG
AAAATGGCGACCTCGATGAGAGAGTTCCTCGGCCGTGAGGTTTCTCTGTCAGAAGTCTTCCACGCCCTTATTAGGAATCT
CGACCACTGGTACAGACTCTTTCTCAATGGACGATATGCTGAGATAATATCCGCGCTGAAGGAGAGGAGCATTGTCCTCG
GGAAGGAAGTCAAGATAATCGACGATGAAACAGAACTCGTCGGCAGGGCTGTGGACATAGATGTGGACGGCGCGCTGATC
CTCGAAACACCAAAGGGCAGAGTGCGGGTGCTCCATGGCGACGTTTCTCTCAGATTTCTCTAA

Upstream 100 bases:

>100_bases
ATCTTTTGGTCTGAGGTTGGTCTTGCTGGTGAGCTTTATCATCAATCCTCTGTCAGGCTTCACTTCGTCGCCGGCGAACC
TGGCGAGGCCGGGGAGCATC

Downstream 100 bases:

>100_bases
ATTTTCCACGATTTTCTGTCATATTGTCAACTTTTTTTGGTAAAAGTTAATATAATGTCGCTATTAATGCTATTTCGACG
TCATTCTTAAAGAGGTGAAG

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTPSTPARITFLMIIFGFTCSQCSSKSVGPCSKPWSIANMSVLPFLRKNLFILLLIPILWYASILITLGDIWFGGFKVFF
DGKLFKSRMRSSAGGLMSGLGSEIIGRKIIHFHEVDSTNEYAKLIALDEREGTVVVADRQTAGKGRKGRTWASPEGGLWM
SVILKPKAEPKHLSKLVFIGALAVVDTLWKFGIEAGIKWPNDVWVNGRKICGVLTEGRIGEFVILGIGLNVNNDAPEELR
KMATSMREFLGREVSLSEVFHALIRNLDHWYRLFLNGRYAEIISALKERSIVLGKEVKIIDDETELVGRAVDIDVDGALI
LETPKGRVRVLHGDVSLRFL

Sequences:

>Translated_340_residues
MTPSTPARITFLMIIFGFTCSQCSSKSVGPCSKPWSIANMSVLPFLRKNLFILLLIPILWYASILITLGDIWFGGFKVFF
DGKLFKSRMRSSAGGLMSGLGSEIIGRKIIHFHEVDSTNEYAKLIALDEREGTVVVADRQTAGKGRKGRTWASPEGGLWM
SVILKPKAEPKHLSKLVFIGALAVVDTLWKFGIEAGIKWPNDVWVNGRKICGVLTEGRIGEFVILGIGLNVNNDAPEELR
KMATSMREFLGREVSLSEVFHALIRNLDHWYRLFLNGRYAEIISALKERSIVLGKEVKIIDDETELVGRAVDIDVDGALI
LETPKGRVRVLHGDVSLRFL
>Mature_339_residues
TPSTPARITFLMIIFGFTCSQCSSKSVGPCSKPWSIANMSVLPFLRKNLFILLLIPILWYASILITLGDIWFGGFKVFFD
GKLFKSRMRSSAGGLMSGLGSEIIGRKIIHFHEVDSTNEYAKLIALDEREGTVVVADRQTAGKGRKGRTWASPEGGLWMS
VILKPKAEPKHLSKLVFIGALAVVDTLWKFGIEAGIKWPNDVWVNGRKICGVLTEGRIGEFVILGIGLNVNNDAPEELRK
MATSMREFLGREVSLSEVFHALIRNLDHWYRLFLNGRYAEIISALKERSIVLGKEVKIIDDETELVGRAVDIDVDGALIL
ETPKGRVRVLHGDVSLRFL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=232, Percent_Identity=29.3103448275862, Blast_Score=85, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24644246, Length=263, Percent_Identity=26.615969581749, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37846; Mature: 37715

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSTPARITFLMIIFGFTCSQCSSKSVGPCSKPWSIANMSVLPFLRKNLFILLLIPILW
CCCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YASILITLGDIWFGGFKVFFDGKLFKSRMRSSAGGLMSGLGSEIIGRKIIHFHEVDSTNE
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCC
YAKLIALDEREGTVVVADRQTAGKGRKGRTWASPEGGLWMSVILKPKAEPKHLSKLVFIG
EEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ALAVVDTLWKFGIEAGIKWPNDVWVNGRKICGVLTEGRIGEFVILGIGLNVNNDAPEELR
HHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHH
KMATSMREFLGREVSLSEVFHALIRNLDHWYRLFLNGRYAEIISALKERSIVLGKEVKII
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCEEEEE
DDETELVGRAVDIDVDGALILETPKGRVRVLHGDVSLRFL
CCHHHHHCEEEEEECCCEEEEECCCCCEEEEEECEEEEEC
>Mature Secondary Structure 
TPSTPARITFLMIIFGFTCSQCSSKSVGPCSKPWSIANMSVLPFLRKNLFILLLIPILW
CCCCCHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YASILITLGDIWFGGFKVFFDGKLFKSRMRSSAGGLMSGLGSEIIGRKIIHFHEVDSTNE
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEECCCCCC
YAKLIALDEREGTVVVADRQTAGKGRKGRTWASPEGGLWMSVILKPKAEPKHLSKLVFIG
EEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ALAVVDTLWKFGIEAGIKWPNDVWVNGRKICGVLTEGRIGEFVILGIGLNVNNDAPEELR
HHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHH
KMATSMREFLGREVSLSEVFHALIRNLDHWYRLFLNGRYAEIISALKERSIVLGKEVKII
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCEEEEE
DDETELVGRAVDIDVDGALILETPKGRVRVLHGDVSLRFL
CCHHHHHCEEEEEECCCEEEEECCCCCEEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]