Click here to switch to the map view.

The map label for this gene is cobB [H]

Identifier: 212223968

GI number: 212223968

Start: 759541

End: 760293

Strand: Reverse

Name: cobB [H]

Synonym: TON_0819

Alternate gene names: 212223968

Gene position: 760293-759541 (Counterclockwise)

Preceding gene: 212223979

Following gene: 212223967

Centisome position: 41.15

GC content: 54.58

Gene sequence:

>753_bases
ATGATAGAGGAAGCCTCAAAACTGCTCGCCCGCTCGAGGTTTGCCATAGCCTTTACCGGGGCTGGAATAAGCGCAGAGAG
CGGTGTTCCAACCTTCAGGGGCTTTAACGGCCTCTGGAAGAAGCACAGGCCGGAGGAGCTGGCAACGCCAGAAGCCTTCA
GGAAGGACCCTCACCTCGTCTGGAGCTTCTACAAGTGGAGAATGGGGCTCATAATGAAAGCCAGACCCAACAGGGCTCAT
TACGCTTTAGCTGAGCTCGAGGAGATGGGCATTTTGAAGGCTGTGATAACCCAGAATGTTGACGACCTCCATCGTGAGGC
CGGCACAAAAAACCTCATCGAGCTCCACGGCAACATCTTTCGCGTTAGGTGTACTTCCTGTGGCTATGAGGAAAACCTCA
AGGAGAATGGTCGCTTGGAAGAGTTCCTGGTCCAAAAAGACCTGCCAAAGTGCCCAAACTGCGATTCGCTTCTGAGGCCT
GACGTGGTGTGGTTTGGCGAGCCGCTGCCGAGGAAAGCCCTGGACGAGGCCTTTAAGCTCGCGGAGAAGGCAGACCTGGT
TCTAGTGATTGGTACCAGCGGTGTTGTCTATCCCGCAGCTTACATCCCCCAGATCGTCAGGGAAACTGGGGGCAAAGTCA
TTGAGGTAAATCCTGAGGAAAGCGGGATCACGCCCATAGCCGACGTCTTCCTGCGCTGTCCGGCTGGTGAGGCTATGGAA
AAGTTGATGAAGAGGATTAAGGGGCTGATATGA

Upstream 100 bases:

>100_bases
GCACGAAAATGTGAAACCTGCTTCAAAGATTATTCGGCGTAGATGGGAAAAGGGCTCAAGGTCAGGCATGATTTTTAAAG
ATAGCCGAGTAAGTATAATC

Downstream 100 bases:

>100_bases
TGGGTGGAAAGGTTCTCCTCATTGGCTTTAATGAGGATGAGGTCGAAAAGATAATGGATGTCCTAAACGGCCTGGATGTC
CTTGAGGTTCCCGAGTATTG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIEEASKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLVWSFYKWRMGLIMKARPNRAH
YALAELEEMGILKAVITQNVDDLHREAGTKNLIELHGNIFRVRCTSCGYEENLKENGRLEEFLVQKDLPKCPNCDSLLRP
DVVWFGEPLPRKALDEAFKLAEKADLVLVIGTSGVVYPAAYIPQIVRETGGKVIEVNPEESGITPIADVFLRCPAGEAME
KLMKRIKGLI

Sequences:

>Translated_250_residues
MIEEASKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLVWSFYKWRMGLIMKARPNRAH
YALAELEEMGILKAVITQNVDDLHREAGTKNLIELHGNIFRVRCTSCGYEENLKENGRLEEFLVQKDLPKCPNCDSLLRP
DVVWFGEPLPRKALDEAFKLAEKADLVLVIGTSGVVYPAAYIPQIVRETGGKVIEVNPEESGITPIADVFLRCPAGEAME
KLMKRIKGLI
>Mature_250_residues
MIEEASKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLVWSFYKWRMGLIMKARPNRAH
YALAELEEMGILKAVITQNVDDLHREAGTKNLIELHGNIFRVRCTSCGYEENLKENGRLEEFLVQKDLPKCPNCDSLLRP
DVVWFGEPLPRKALDEAFKLAEKADLVLVIGTSGVVYPAAYIPQIVRETGGKVIEVNPEESGITPIADVFLRCPAGEAME
KLMKRIKGLI

Specific function: Modulates the activity of chromatin proteins, thereby having an effect on transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=257, Percent_Identity=42.0233463035019, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI13787215, Length=244, Percent_Identity=44.2622950819672, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI300795542, Length=252, Percent_Identity=42.0634920634921, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=31.1023622047244, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI6912662, Length=225, Percent_Identity=32, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI13775602, Length=203, Percent_Identity=35.4679802955665, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI13775600, Length=203, Percent_Identity=35.4679802955665, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI63054862, Length=201, Percent_Identity=34.3283582089552, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI6912660, Length=204, Percent_Identity=33.8235294117647, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7657575, Length=219, Percent_Identity=31.0502283105023, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI300797705, Length=194, Percent_Identity=35.0515463917526, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI300797597, Length=254, Percent_Identity=29.1338582677165, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI7706712, Length=206, Percent_Identity=27.1844660194175, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI215982798, Length=162, Percent_Identity=30.8641975308642, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI308199517, Length=240, Percent_Identity=40.8333333333333, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17541892, Length=219, Percent_Identity=31.5068493150685, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=208, Percent_Identity=34.6153846153846, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=214, Percent_Identity=32.2429906542056, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990487, Length=216, Percent_Identity=31.9444444444444, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17505510, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=214, Percent_Identity=35.981308411215, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324504, Length=246, Percent_Identity=26.4227642276423, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324599, Length=285, Percent_Identity=25.6140350877193, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571445, Length=258, Percent_Identity=29.8449612403101, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137536, Length=253, Percent_Identity=29.2490118577075, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648389, Length=201, Percent_Identity=31.3432835820896, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24645650, Length=252, Percent_Identity=28.1746031746032, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571443, Length=194, Percent_Identity=28.8659793814433, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571441, Length=194, Percent_Identity=28.8659793814433, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 28032; Mature: 28032

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEASKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLV
CCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHCCCCHHH
WSFYKWRMGLIMKARPNRAHYALAELEEMGILKAVITQNVDDLHREAGTKNLIELHGNIF
HHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCEE
RVRCTSCGYEENLKENGRLEEFLVQKDLPKCPNCDSLLRPDVVWFGEPLPRKALDEAFKL
EEEECCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCCHHHHHHHHHH
AEKADLVLVIGTSGVVYPAAYIPQIVRETGGKVIEVNPEESGITPIADVFLRCPAGEAME
HHHCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHH
KLMKRIKGLI
HHHHHHHCCC
>Mature Secondary Structure
MIEEASKLLARSRFAIAFTGAGISAESGVPTFRGFNGLWKKHRPEELATPEAFRKDPHLV
CCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHCCCCHHH
WSFYKWRMGLIMKARPNRAHYALAELEEMGILKAVITQNVDDLHREAGTKNLIELHGNIF
HHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCEE
RVRCTSCGYEENLKENGRLEEFLVQKDLPKCPNCDSLLRPDVVWFGEPLPRKALDEAFKL
EEEECCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCCHHHHHHHHHH
AEKADLVLVIGTSGVVYPAAYIPQIVRETGGKVIEVNPEESGITPIADVFLRCPAGEAME
HHHCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCHHHH
KLMKRIKGLI
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA