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The map label for this gene is dys [H]

Identifier: 212223850

GI number: 212223850

Start: 654076

End: 655083

Strand: Reverse

Name: dys [H]

Synonym: TON_0701

Alternate gene names: 212223850

Gene position: 655083-654076 (Counterclockwise)

Preceding gene: 212223851

Following gene: 212223849

Centisome position: 35.46

GC content: 54.56

Gene sequence:

>1008_bases
ATGACCGAGCCAAAAGATATAGTCCTCAAGGAATCGGAAGAGATTGAGGGAACGCCCATCGAGGGGCCGTGGCTGGATGA
AGTGAGGATCCTTGAAGAGGTCATCGATTACTATCACCGCATAGGCTTCCAGGCGACTCACCTTGGAAGGGCCATCGAAA
TATGGAAAAAGGTGGAGGAAAAGCGCGCCAGCGGCGAAGAGGTCAGGGTCTTCCTCGGCTACACTTCCAACATAGTCTCT
TCTGGCCTCAGAGAGCTGATAGCATGGCTGGTCAAGGAGGGCAAGGTGGACGTCATCGTAACAACGGCCGGCGGCGTTGA
GGAGGACTTCATAAAGGCCCTAAAGCCGTTCATTCTGGGCGACTGGTACGTCAACGACGCGGAGATGAGAGAGAAGGGCA
TAAACAGAATAGGCAACATCTTCGTGCCCAACGACAGGTACATTGAATTCGAGAAGTACATGATACCCTTCTTCGAACGG
GTTCTTGAGATGGAAAAGGAGCGCGGGAAGCCGCTGACGGCGAGCGAGTTCATCTACGAGATGGGCCGCTTCATGGACGA
AAAGCTCGGGAAGGAGAAGGAGCGCTCGATAATCTACTGGGCCTACAAGCGGAACGTCCCGATATTCTGCCCTGCTATAA
CCGACGGCTCAATAGGGGACATGCTCTACTTCTTCAAGGAGGAGCGCGGAGACAGGGAGCTTATCATAGACGTAGCCAAC
GACATCGTGAAGCTTAACAACCTGGCTGTCACAGCGAAGGAAACCGCTTCGATAATCCTTGGAGGTTCCCTGCCGAAGCA
TGCAATAATAAACGCCAACCTCTTCAGAGGAGGAACGGACTACGCGATATACGTCACCACTGCCATTCCCTGGGATGGCT
CGCTTAGCGGCGCGCCGCCGAGCGAAGGAGTGAGCTGGGGCAAGATAAGGGCTAAGGCTGACTACGTCGAGATATGGGCC
GATGCGACGCTCGTCTTTCCGGTGCTGGTGTGGAAGGTGATGCGCTGA

Upstream 100 bases:

>100_bases
GCAGGGTCTTTCCCGTTGCTATGACCGGCAGGCTCATAGCGGAGGTTCTCAGCGGTAAGTGCTGAGCAAAGTTTATACGC
CCTCCCCTGAACCCACTATC

Downstream 100 bases:

>100_bases
CATTTTGTTCTTTTCTCTGAACAATATGCACGCATATCGTCCCCGCGTGAGGACAAGATTTATAAATAAAGAATCCAACT
TCAAAACATGGACGAGAGGA

Product: putative deoxyhypusine synthase

Products: NA

Alternate protein names: DHS [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTEPKDIVLKESEEIEGTPIEGPWLDEVRILEEVIDYYHRIGFQATHLGRAIEIWKKVEEKRASGEEVRVFLGYTSNIVS
SGLRELIAWLVKEGKVDVIVTTAGGVEEDFIKALKPFILGDWYVNDAEMREKGINRIGNIFVPNDRYIEFEKYMIPFFER
VLEMEKERGKPLTASEFIYEMGRFMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDVAN
DIVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPPSEGVSWGKIRAKADYVEIWA
DATLVFPVLVWKVMR

Sequences:

>Translated_335_residues
MTEPKDIVLKESEEIEGTPIEGPWLDEVRILEEVIDYYHRIGFQATHLGRAIEIWKKVEEKRASGEEVRVFLGYTSNIVS
SGLRELIAWLVKEGKVDVIVTTAGGVEEDFIKALKPFILGDWYVNDAEMREKGINRIGNIFVPNDRYIEFEKYMIPFFER
VLEMEKERGKPLTASEFIYEMGRFMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDVAN
DIVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPPSEGVSWGKIRAKADYVEIWA
DATLVFPVLVWKVMR
>Mature_334_residues
TEPKDIVLKESEEIEGTPIEGPWLDEVRILEEVIDYYHRIGFQATHLGRAIEIWKKVEEKRASGEEVRVFLGYTSNIVSS
GLRELIAWLVKEGKVDVIVTTAGGVEEDFIKALKPFILGDWYVNDAEMREKGINRIGNIFVPNDRYIEFEKYMIPFFERV
LEMEKERGKPLTASEFIYEMGRFMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDVAND
IVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPPSEGVSWGKIRAKADYVEIWAD
ATLVFPVLVWKVMR

Specific function: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine

COG id: COG1899

COG function: function code O; Deoxyhypusine synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the deoxyhypusine synthase family [H]

Homologues:

Organism=Homo sapiens, GI4503325, Length=343, Percent_Identity=42.8571428571429, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI7108338, Length=343, Percent_Identity=36.1516034985423, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI7108340, Length=343, Percent_Identity=34.1107871720117, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17536927, Length=318, Percent_Identity=43.7106918238994, Blast_Score=274, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6321859, Length=362, Percent_Identity=39.7790055248619, Blast_Score=266, Evalue=3e-72,
Organism=Drosophila melanogaster, GI45550569, Length=352, Percent_Identity=44.3181818181818, Blast_Score=286, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002773
- InterPro:   IPR022899 [H]

Pfam domain/function: PF01916 DS [H]

EC number: =2.5.1.46 [H]

Molecular weight: Translated: 38173; Mature: 38042

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPKDIVLKESEEIEGTPIEGPWLDEVRILEEVIDYYHRIGFQATHLGRAIEIWKKVEE
CCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KRASGEEVRVFLGYTSNIVSSGLRELIAWLVKEGKVDVIVTTAGGVEEDFIKALKPFILG
HHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
DWYVNDAEMREKGINRIGNIFVPNDRYIEFEKYMIPFFERVLEMEKERGKPLTASEFIYE
CEEECHHHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MGRFMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDVAN
HHHHHHHHHCCHHHCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEHHH
DIVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPP
HHHHHCCEEEEECCCEEEEECCCCCCCEEEECEEECCCCCEEEEEEEECCCCCCCCCCCC
SEGVSWGKIRAKADYVEIWADATLVFPVLVWKVMR
CCCCCCCEEEECCCEEEEHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEPKDIVLKESEEIEGTPIEGPWLDEVRILEEVIDYYHRIGFQATHLGRAIEIWKKVEE
CCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
KRASGEEVRVFLGYTSNIVSSGLRELIAWLVKEGKVDVIVTTAGGVEEDFIKALKPFILG
HHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCC
DWYVNDAEMREKGINRIGNIFVPNDRYIEFEKYMIPFFERVLEMEKERGKPLTASEFIYE
CEEECHHHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MGRFMDEKLGKEKERSIIYWAYKRNVPIFCPAITDGSIGDMLYFFKEERGDRELIIDVAN
HHHHHHHHHCCHHHCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEEHHH
DIVKLNNLAVTAKETASIILGGSLPKHAIINANLFRGGTDYAIYVTTAIPWDGSLSGAPP
HHHHHCCEEEEECCCEEEEECCCCCCCEEEECEEECCCCCEEEEEEEECCCCCCCCCCCC
SEGVSWGKIRAKADYVEIWADATLVFPVLVWKVMR
CCCCCCCEEEECCCEEEEHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA