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The map label for this gene is gltD [H]

Identifier: 212223851

GI number: 212223851

Start: 655119

End: 656165

Strand: Reverse

Name: gltD [H]

Synonym: TON_0702

Alternate gene names: 212223851

Gene position: 656165-655119 (Counterclockwise)

Preceding gene: 212223853

Following gene: 212223850

Centisome position: 35.51

GC content: 55.21

Gene sequence:

>1047_bases
GTGAGGTTCTACATCTGTGAGAAGAGAGAAGAAGTTAAGCCTTTTAAAATAGGGATTATCGGAGCGGGACCTGCAGGCCT
GGCCGCCGCGGGTTATCTGGCGTGCAGGGGTTATGAAGTTCACGTCTACGACAAGATGCCTGAGGGCGGTGGAATGGTCG
CCTTTGGCATTCCCGAGGCAAGGATTCCAATAAGGGCCGTCAGAGAGGGCGTCGAGGCTCTTGAGCAGCTCGGCGTTCAA
TTTCACTTCCGAACGAAGGTAGTCTATGATTCCCCAAAGGAGCTCGGCGACGAGTGGGCCGAGCACTTCGTTTCGATTGA
AAAGCTTTTGAGCGAGTTCGATGCTCTCATAATAGCGACCGGTGCTTGGAAGCCGAGAAAGCTGAAGGTTCCCGGTATTG
GGCTTCCTGGAGTCTACGATGCCCTCAGCCTGCTCCACAGCATAAAGATGGCCCGCATAGGCTATTACTCCTGGGAAAGA
ATCCCGGACCTTAAGGGGAAGCATCTCGTAGTGATCGGAGCCGGCTACACAGCAGTTGATGTCGCCATGGAGGGTCGTCT
TCTGGGTGCGGAAAAAATAACCATGGTCTACCGCCGCTCCCTCGAGCACAGCTACGCTAAGGCCGAGATAAGAAAGCTCA
TCGCAGAGGGCGTCGAGTTCATAGAGCACGCGACTCCGGTCAGGATACTAGGAGAGGAGAAAGTGGAGGGTGTTGAATTC
GCTAAAACAAAAATAGTCGGGGGAAACGTCGTGACGACGGACGAGCACTTTGTCATAGACGCAGACGTCGTTGCCTATGC
CATCGGCCAGCTGCCCACGAGCCCAATAAAGGAAATCGTCTGCGCCAGCGAAAAGGTGCTGGAGGAGGCGGGAATATTCT
TCGCCGGTGACGTCGTAACCCCAAGGAACATAGGCACCGCCATAAGAGAGGGAAGAGCCGTCGCTGAGAGGATAGAGGAG
TGGCTCCTAAGGAAGGCACCGCGCAGGGTCTTTCCCGTTGCTATGACCGGCAGGCTCATAGCGGAGGTTCTCAGCGGTAA
GTGCTGA

Upstream 100 bases:

>100_bases
ACCTAATTCGTCTTATCGTCAGTGTTTCGAGAGAAAACAACTTAAGGTGCAAAACCTTTGGTTTTAGACAGACTTCTTTA
AAAATTTTGGAGGTGATTGT

Downstream 100 bases:

>100_bases
GCAAAGTTTATACGCCCTCCCCTGAACCCACTATCATGACCGAGCCAAAAGATATAGTCCTCAAGGAATCGGAAGAGATT
GAGGGAACGCCCATCGAGGG

Product: putative glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MRFYICEKREEVKPFKIGIIGAGPAGLAAAGYLACRGYEVHVYDKMPEGGGMVAFGIPEARIPIRAVREGVEALEQLGVQ
FHFRTKVVYDSPKELGDEWAEHFVSIEKLLSEFDALIIATGAWKPRKLKVPGIGLPGVYDALSLLHSIKMARIGYYSWER
IPDLKGKHLVVIGAGYTAVDVAMEGRLLGAEKITMVYRRSLEHSYAKAEIRKLIAEGVEFIEHATPVRILGEEKVEGVEF
AKTKIVGGNVVTTDEHFVIDADVVAYAIGQLPTSPIKEIVCASEKVLEEAGIFFAGDVVTPRNIGTAIREGRAVAERIEE
WLLRKAPRRVFPVAMTGRLIAEVLSGKC

Sequences:

>Translated_348_residues
MRFYICEKREEVKPFKIGIIGAGPAGLAAAGYLACRGYEVHVYDKMPEGGGMVAFGIPEARIPIRAVREGVEALEQLGVQ
FHFRTKVVYDSPKELGDEWAEHFVSIEKLLSEFDALIIATGAWKPRKLKVPGIGLPGVYDALSLLHSIKMARIGYYSWER
IPDLKGKHLVVIGAGYTAVDVAMEGRLLGAEKITMVYRRSLEHSYAKAEIRKLIAEGVEFIEHATPVRILGEEKVEGVEF
AKTKIVGGNVVTTDEHFVIDADVVAYAIGQLPTSPIKEIVCASEKVLEEAGIFFAGDVVTPRNIGTAIREGRAVAERIEE
WLLRKAPRRVFPVAMTGRLIAEVLSGKC
>Mature_348_residues
MRFYICEKREEVKPFKIGIIGAGPAGLAAAGYLACRGYEVHVYDKMPEGGGMVAFGIPEARIPIRAVREGVEALEQLGVQ
FHFRTKVVYDSPKELGDEWAEHFVSIEKLLSEFDALIIATGAWKPRKLKVPGIGLPGVYDALSLLHSIKMARIGYYSWER
IPDLKGKHLVVIGAGYTAVDVAMEGRLLGAEKITMVYRRSLEHSYAKAEIRKLIAEGVEFIEHATPVRILGEEKVEGVEF
AKTKIVGGNVVTTDEHFVIDADVVAYAIGQLPTSPIKEIVCASEKVLEEAGIFFAGDVVTPRNIGTAIREGRAVAERIEE
WLLRKAPRRVFPVAMTGRLIAEVLSGKC

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=342, Percent_Identity=25.4385964912281, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789606, Length=339, Percent_Identity=29.7935103244838, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788811, Length=336, Percent_Identity=29.7619047619048, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87082180, Length=221, Percent_Identity=32.1266968325792, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788468, Length=321, Percent_Identity=27.1028037383178, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789243, Length=202, Percent_Identity=31.6831683168317, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17570289, Length=355, Percent_Identity=26.7605633802817, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320030, Length=167, Percent_Identity=31.1377245508982, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665547, Length=179, Percent_Identity=28.4916201117318, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24665543, Length=179, Percent_Identity=28.4916201117318, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28574881, Length=179, Percent_Identity=28.4916201117318, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24665539, Length=179, Percent_Identity=28.4916201117318, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640763, Length=249, Percent_Identity=28.5140562248996, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI18858217, Length=249, Percent_Identity=28.5140562248996, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 38288; Mature: 38288

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFYICEKREEVKPFKIGIIGAGPAGLAAAGYLACRGYEVHVYDKMPEGGGMVAFGIPEA
CEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHEECCEEEEEEECCCCCCCEEEECCCCC
RIPIRAVREGVEALEQLGVQFHFRTKVVYDSPKELGDEWAEHFVSIEKLLSEFDALIIAT
CCCHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
GAWKPRKLKVPGIGLPGVYDALSLLHSIKMARIGYYSWERIPDLKGKHLVVIGAGYTAVD
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCEEEE
VAMEGRLLGAEKITMVYRRSLEHSYAKAEIRKLIAEGVEFIEHATPVRILGEEKVEGVEF
HEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
AKTKIVGGNVVTTDEHFVIDADVVAYAIGQLPTSPIKEIVCASEKVLEEAGIFFAGDVVT
HHEEEECCCEEECCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
PRNIGTAIREGRAVAERIEEWLLRKAPRRVFPVAMTGRLIAEVLSGKC
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFYICEKREEVKPFKIGIIGAGPAGLAAAGYLACRGYEVHVYDKMPEGGGMVAFGIPEA
CEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHEECCEEEEEEECCCCCCCEEEECCCCC
RIPIRAVREGVEALEQLGVQFHFRTKVVYDSPKELGDEWAEHFVSIEKLLSEFDALIIAT
CCCHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
GAWKPRKLKVPGIGLPGVYDALSLLHSIKMARIGYYSWERIPDLKGKHLVVIGAGYTAVD
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCEEEE
VAMEGRLLGAEKITMVYRRSLEHSYAKAEIRKLIAEGVEFIEHATPVRILGEEKVEGVEF
HEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHH
AKTKIVGGNVVTTDEHFVIDADVVAYAIGQLPTSPIKEIVCASEKVLEEAGIFFAGDVVT
HHEEEECCCEEECCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
PRNIGTAIREGRAVAERIEEWLLRKAPRRVFPVAMTGRLIAEVLSGKC
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]