Click here to switch to the map view.

The map label for this gene is 212223785

Identifier: 212223785

GI number: 212223785

Start: 594677

End: 595504

Strand: Reverse

Name: 212223785

Synonym: TON_0637

Alternate gene names: NA

Gene position: 595504-594677 (Counterclockwise)

Preceding gene: 212223786

Following gene: 212223783

Centisome position: 32.23

GC content: 53.02

Gene sequence:

>828_bases
ATGCTTCCTCCGGAAATTCGCTCCATCCTCGAAGAGATGCGGGCCGAGCGCATAAGAGGCGCGAGTTACCTGGCCAAGAG
AGGCGCAGAAGTATATATAAAGCTCGCTGAGCTTTTAAGCGGCGATGGACTACGAGAAGCTCTTAAGGATATGAGAGAAG
AGATTCCTGCCGTGAACAGGACGATGGCCTCACTCTACAACCTGGCCAGATTCATACCCATCACGGATAACCCCGACCTT
GTAATATCAAAGGCCGAGGAATTCATCCGCCTGAGCGAGGAAGCCAAAAGGGAGATCGGCAACATCGGGAGCGAGCTGAT
AGACGAGAATGAGGTAATAATAACCCACTCCTTCTCCTCGGCTGTTCTGGAGATTTTTAAGGCCGCGAAGAGGAAGGGCA
AGCGCTTCAAGGTAATTCTAACGGAGAGCGCACCGGACTACGAGGGGCTGGCCTTAGCCGGGGAGCTTGAGGCCTTAGAA
ATTCCCTTCGAGGTTATCACCGACGCTCAGATAGGCATCTTTGTCAAAAAAGCCACCCTGGCCTTCGTCGGTGCCGATAA
CGTTACGCGTGATGGGGCGGTTGTCAACAAGGCTGGCACGTACCTCCTTGCCCTAGCCTGTCATGACAATGGCGTTCCTT
TCTACGTCGCGGCGGAGAGCTTTAAACTCCATCCTGAGCTGAACTCCGAGGAGGTTGAAATAGTCGAGAGACCCTACGCG
AGACAGGGCTACCGCGTCAGGAACTATCTCTTTGACATCACGCCCTGGAAATACGTTAGGGGGATAGTAACTGAACTCGG
AATTCTGGTGCCTCCGAAGGAGATTTAA

Upstream 100 bases:

>100_bases
TCGAGGAGGGCTGGTAGCGAAAGAGTGATAAGGATAAACGGGCAATTCTATCGGGTCAAATCCAGCCGAAGGGGAACCAA
CCGCTGTTGTGGTGTGAGCC

Downstream 100 bases:

>100_bases
AGTCCTCTTTTCTTGTCATCTTCACAATCTAGAAAGAACGGAAGGAAAGAAAAGAAATCAAATTTCGTGGGCCAGCCAGA
GAAGGACGCCCTTGACGAAC

Product: translation initiation factor IF-2B subunit alpha

Products: NA

Alternate protein names: eIF-2B GDP-GTP exchange factor [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLPPEIRSILEEMRAERIRGASYLAKRGAEVYIKLAELLSGDGLREALKDMREEIPAVNRTMASLYNLARFIPITDNPDL
VISKAEEFIRLSEEAKREIGNIGSELIDENEVIITHSFSSAVLEIFKAAKRKGKRFKVILTESAPDYEGLALAGELEALE
IPFEVITDAQIGIFVKKATLAFVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYVAAESFKLHPELNSEEVEIVERPYA
RQGYRVRNYLFDITPWKYVRGIVTELGILVPPKEI

Sequences:

>Translated_275_residues
MLPPEIRSILEEMRAERIRGASYLAKRGAEVYIKLAELLSGDGLREALKDMREEIPAVNRTMASLYNLARFIPITDNPDL
VISKAEEFIRLSEEAKREIGNIGSELIDENEVIITHSFSSAVLEIFKAAKRKGKRFKVILTESAPDYEGLALAGELEALE
IPFEVITDAQIGIFVKKATLAFVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYVAAESFKLHPELNSEEVEIVERPYA
RQGYRVRNYLFDITPWKYVRGIVTELGILVPPKEI
>Mature_275_residues
MLPPEIRSILEEMRAERIRGASYLAKRGAEVYIKLAELLSGDGLREALKDMREEIPAVNRTMASLYNLARFIPITDNPDL
VISKAEEFIRLSEEAKREIGNIGSELIDENEVIITHSFSSAVLEIFKAAKRKGKRFKVILTESAPDYEGLALAGELEALE
IPFEVITDAQIGIFVKKATLAFVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYVAAESFKLHPELNSEEVEIVERPYA
RQGYRVRNYLFDITPWKYVRGIVTELGILVPPKEI

Specific function: Catalyzes the exchange of initiation factor 2-bound GDP for GTP [H]

COG id: COG1184

COG function: function code J; Translation initiation factor 2B subunit, eIF-2B alpha/beta/delta family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eIF-2B alpha/beta/delta subunits family [H]

Homologues:

Organism=Homo sapiens, GI4503503, Length=251, Percent_Identity=30.6772908366534, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI72534748, Length=222, Percent_Identity=30.6306306306306, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI7657058, Length=127, Percent_Identity=37.007874015748, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI23943880, Length=146, Percent_Identity=36.986301369863, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI78000158, Length=193, Percent_Identity=24.3523316062176, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI78000156, Length=193, Percent_Identity=24.3523316062176, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI187607323, Length=193, Percent_Identity=24.3523316062176, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557123, Length=199, Percent_Identity=28.1407035175879, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557462, Length=172, Percent_Identity=30.8139534883721, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322878, Length=200, Percent_Identity=30.5, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358605, Length=241, Percent_Identity=27.3858921161826, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364091, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24659399, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24659407, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922816, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21357667, Length=216, Percent_Identity=28.2407407407407, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651647, Length=216, Percent_Identity=28.2407407407407, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000649 [H]

Pfam domain/function: PF01008 IF-2B [H]

EC number: NA

Molecular weight: Translated: 30725; Mature: 30725

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPPEIRSILEEMRAERIRGASYLAKRGAEVYIKLAELLSGDGLREALKDMREEIPAVNR
CCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
TMASLYNLARFIPITDNPDLVISKAEEFIRLSEEAKREIGNIGSELIDENEVIITHSFSS
HHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
AVLEIFKAAKRKGKRFKVILTESAPDYEGLALAGELEALEIPFEVITDAQIGIFVKKATL
HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCHHEECCHHHHCCCHHEEEEEEEEE
AFVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYVAAESFKLHPELNSEEVEIVERPYA
EEECCCCCCCCCCEEECCCCEEEEEEEECCCCEEEEEECCEEECCCCCCCHHHHHHCCHH
RQGYRVRNYLFDITPWKYVRGIVTELGILVPPKEI
HCCCEEHHHEEECCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLPPEIRSILEEMRAERIRGASYLAKRGAEVYIKLAELLSGDGLREALKDMREEIPAVNR
CCCHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
TMASLYNLARFIPITDNPDLVISKAEEFIRLSEEAKREIGNIGSELIDENEVIITHSFSS
HHHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
AVLEIFKAAKRKGKRFKVILTESAPDYEGLALAGELEALEIPFEVITDAQIGIFVKKATL
HHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCHHEECCHHHHCCCHHEEEEEEEEE
AFVGADNVTRDGAVVNKAGTYLLALACHDNGVPFYVAAESFKLHPELNSEEVEIVERPYA
EEECCCCCCCCCCEEECCCCEEEEEEEECCCCEEEEEECCEEECCCCCCCHHHHHHCCHH
RQGYRVRNYLFDITPWKYVRGIVTELGILVPPKEI
HCCCEEHHHEEECCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]