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The map label for this gene is ribH

Identifier: 212223755

GI number: 212223755

Start: 559928

End: 560386

Strand: Reverse

Name: ribH

Synonym: TON_0607

Alternate gene names: 212223755

Gene position: 560386-559928 (Counterclockwise)

Preceding gene: 212223756

Following gene: 212223754

Centisome position: 30.33

GC content: 49.24

Gene sequence:

>459_bases
TTGGAGGTTAGGGTGTTTGAGGGCGAATTTAAGGGAGATGGCCTCAGCATGGCGATAGTTGTTGCCAGGTTCAACGACCT
CTTGACGGATGAACTACTGAGCGGAGCCATAGACTGCTTCGAGAGGCACGGTGTGGAAAGAGTGGACGTCTTCAAGGTTC
CAGGATCCTTTGAGATCCCAATTATAGCCAAAAAGCTCGCCGAGAGTGGAAAATACGATGCCATTTTGGCTTTAGGTGTT
GTTGTTAGAGGTGAGACTAAGCACTTCGATCTCGTTGCCAACGAAGTATCCAAGGGGGTCGCCCAAGCTGGCCTGATCAC
TGGTGTTCCAGTTATCTTCGGTGTCATAGCCGTTGAGGACGAGCTTCAAGGATTGAACAGGGCTGGAATAAAGAGCAATA
AAGGGTTTGAGTATGCGATGGCAGCTTTGGAGATGGCGAACTTGATGAAAAAGCTGTGA

Upstream 100 bases:

>100_bases
AGAAGCTTGGCCACAAGCTTCCTTTTGAAGTTTGATTAATTCAGGATCATGTAATCACGTCATTATGCAATTACCGAAGT
AACAAACGGAGGTGATGGTA

Downstream 100 bases:

>100_bases
TATAGTTTGTAGTTTTTTATTTTTTAATTTCTGTACTAACATCATTGCAACATTTGCCAGTCTGTAGAAATGACAAAAAT
CATTCGTAAGTAGTTGGCAA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MEVRVFEGEFKGDGLSMAIVVARFNDLLTDELLSGAIDCFERHGVERVDVFKVPGSFEIPIIAKKLAESGKYDAILALGV
VVRGETKHFDLVANEVSKGVAQAGLITGVPVIFGVIAVEDELQGLNRAGIKSNKGFEYAMAALEMANLMKKL

Sequences:

>Translated_152_residues
MEVRVFEGEFKGDGLSMAIVVARFNDLLTDELLSGAIDCFERHGVERVDVFKVPGSFEIPIIAKKLAESGKYDAILALGV
VVRGETKHFDLVANEVSKGVAQAGLITGVPVIFGVIAVEDELQGLNRAGIKSNKGFEYAMAALEMANLMKKL
>Mature_152_residues
MEVRVFEGEFKGDGLSMAIVVARFNDLLTDELLSGAIDCFERHGVERVDVFKVPGSFEIPIIAKKLAESGKYDAILALGV
VVRGETKHFDLVANEVSKGVAQAGLITGVPVIFGVIAVEDELQGLNRAGIKSNKGFEYAMAALEMANLMKKL

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=43.5064935064935, Blast_Score=128, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=32.8859060402685, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_THEON (B6YUQ8)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306991.1
- GeneID:   7016905
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0607
- HOGENOM:   HBG311126
- OMA:   LGLQGAC
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16385; Mature: 16385

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVRVFEGEFKGDGLSMAIVVARFNDLLTDELLSGAIDCFERHGVERVDVFKVPGSFEIP
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
IIAKKLAESGKYDAILALGVVVRGETKHFDLVANEVSKGVAQAGLITGVPVIFGVIAVED
HHHHHHHCCCCCHHEEEEHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQGLNRAGIKSNKGFEYAMAALEMANLMKKL
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVRVFEGEFKGDGLSMAIVVARFNDLLTDELLSGAIDCFERHGVERVDVFKVPGSFEIP
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
IIAKKLAESGKYDAILALGVVVRGETKHFDLVANEVSKGVAQAGLITGVPVIFGVIAVED
HHHHHHHCCCCCHHEEEEHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELQGLNRAGIKSNKGFEYAMAALEMANLMKKL
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA