Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 212223750

GI number: 212223750

Start: 553515

End: 554945

Strand: Reverse

Name: cysS

Synonym: TON_0602

Alternate gene names: 212223750

Gene position: 554945-553515 (Counterclockwise)

Preceding gene: 212223754

Following gene: 212223749

Centisome position: 30.04

GC content: 54.23

Gene sequence:

>1431_bases
ATGGCCATTAGAGTGTACAACACCTTGACGAAGCAGAAGGAGGAGTTCAGGCCCCTGAGAGAAGGGGAGGTCAGGATGTA
CGTCTGCGGTCCCACGGTTTACGATTACACCCACCTCGGTCACGCCAGAACCTACATAGCCTTCGACGTCATCAGGCGCT
ACCTCGAGCACAGAGGTTATACCGTTCTCATGGTCATGAACTTCACTGACATAGACGACAAGATTATCAGGCGCGCCAAC
GAGACCGGCGAAGACCCTAAGGAGCTCGCTGAGAAGTTCCTTCGCTACTTCCTCGAGGATATGAAAGCTTTGAAGGTCAA
ACCGGCCGATATCTATCCGCGCGTTACGGAGCACATTCAGGACATTATAGACTTCGTGAGAAAGCTCCAGGAAAAGGGCT
ACGCCTACGAGGGGAACGATGGGGTATACTTCGAGGTCAGGAAGTTCAAGGACTATGGTAAGCTGAGCGGAATAAAGCTT
GAAGAGCTAGTTAAGGGGGCGAGGGTTGAGCCTGGCGAAGGCAAGAAGAATCCTGAAGACTTCGCCCTCTGGAAGAAGGC
CAAGCCCGGAGAACCTAAATGGGAAAGCCCCTGGGGCGAAGGCAGGCCCGGCTGGCACATCGAGTGCTCCACGATGAGCA
CAAAGTATCTCGGCGAGAGCTTTGACATCCATGGTGGCGGGAACGACCTCATCTTTCCGCACCACGAGAACGAAATTGCC
CAGAGCGAGGCGTGCACCGGCCATGAGTGGGTCAGGTACTGGCTCCATACAGGCTTCGTGATGGTTTCCGGAGAAAAGAT
GAGTAAGAGCCTCGGTAACTTCGTGACGATAAGGGAGCTCCTCCAGAAGTACAGCCCGGAGGTCATACGCTTCTTTGTCC
TCCAGAAGCACTACCGCTCGCCGCTCGACTACACAGAGGAGGGCATCCAGCACGCCAAGAACAACCTTGAGAGGCTCTAC
AACACCCTCGAAAACATCCGCGTGGCGATGGAGAAGGCCGATGTTGCATTCAGATGGGACGAAGAGGAGTTCGAGCTCTA
CGAGACCGTCAGGGAAGCAAGAAAGAAGTTCTACGATGCCATGGACGACGACTTCAACACGGCCGAGGCCCTCAAGGCCG
TCTTTGAGGTCTCCAACGCGGTTAACCGCTACCTCACGAAGGTCGAAAAGCCCAAGGAAAGCGTTCTCAGGAAGGCGCTG
GAGTTCTTCAAGATAGTAAGCGAGGTCTTCGGTCTCTTCGAGGACTACTTCAAGGAGCAGAAAGCCGGTGATGAGGAGGC
ACTCATAGAGCTCCTTGTCTCAGTAAGGGCGGAGCTCAGGAAGCAGCGCAACTTCACTCTGGCGGATAAAATCAGAGACG
AGCTCAAGGCTCTGGGCATACAGCTTGAGGACACGCCACAAGGAACAATATGGAAGAGGATTAGTGTTTGA

Upstream 100 bases:

>100_bases
TCGTGCTCCTACCCCGATTAAGGGAGTTGGTTTTTAAGCTTTCCCACCGGGCGATAACCTAATAAAACGCCTCGGCAAAG
TTCACCCGGTGGTGAGAAAG

Downstream 100 bases:

>100_bases
TTTTTTCTGCCTCTTTTTGCCACCTTCCAGTCAAAACGCTGAGCGTAAGGCTTAAATATAACCTTTCCTTTGCTCCGTTA
AGGGAAATCCAATGAAGAAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAIRVYNTLTKQKEEFRPLREGEVRMYVCGPTVYDYTHLGHARTYIAFDVIRRYLEHRGYTVLMVMNFTDIDDKIIRRAN
ETGEDPKELAEKFLRYFLEDMKALKVKPADIYPRVTEHIQDIIDFVRKLQEKGYAYEGNDGVYFEVRKFKDYGKLSGIKL
EELVKGARVEPGEGKKNPEDFALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIA
QSEACTGHEWVRYWLHTGFVMVSGEKMSKSLGNFVTIRELLQKYSPEVIRFFVLQKHYRSPLDYTEEGIQHAKNNLERLY
NTLENIRVAMEKADVAFRWDEEEFELYETVREARKKFYDAMDDDFNTAEALKAVFEVSNAVNRYLTKVEKPKESVLRKAL
EFFKIVSEVFGLFEDYFKEQKAGDEEALIELLVSVRAELRKQRNFTLADKIRDELKALGIQLEDTPQGTIWKRISV

Sequences:

>Translated_476_residues
MAIRVYNTLTKQKEEFRPLREGEVRMYVCGPTVYDYTHLGHARTYIAFDVIRRYLEHRGYTVLMVMNFTDIDDKIIRRAN
ETGEDPKELAEKFLRYFLEDMKALKVKPADIYPRVTEHIQDIIDFVRKLQEKGYAYEGNDGVYFEVRKFKDYGKLSGIKL
EELVKGARVEPGEGKKNPEDFALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIA
QSEACTGHEWVRYWLHTGFVMVSGEKMSKSLGNFVTIRELLQKYSPEVIRFFVLQKHYRSPLDYTEEGIQHAKNNLERLY
NTLENIRVAMEKADVAFRWDEEEFELYETVREARKKFYDAMDDDFNTAEALKAVFEVSNAVNRYLTKVEKPKESVLRKAL
EFFKIVSEVFGLFEDYFKEQKAGDEEALIELLVSVRAELRKQRNFTLADKIRDELKALGIQLEDTPQGTIWKRISV
>Mature_475_residues
AIRVYNTLTKQKEEFRPLREGEVRMYVCGPTVYDYTHLGHARTYIAFDVIRRYLEHRGYTVLMVMNFTDIDDKIIRRANE
TGEDPKELAEKFLRYFLEDMKALKVKPADIYPRVTEHIQDIIDFVRKLQEKGYAYEGNDGVYFEVRKFKDYGKLSGIKLE
ELVKGARVEPGEGKKNPEDFALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIAQ
SEACTGHEWVRYWLHTGFVMVSGEKMSKSLGNFVTIRELLQKYSPEVIRFFVLQKHYRSPLDYTEEGIQHAKNNLERLYN
TLENIRVAMEKADVAFRWDEEEFELYETVREARKKFYDAMDDDFNTAEALKAVFEVSNAVNRYLTKVEKPKESVLRKALE
FFKIVSEVFGLFEDYFKEQKAGDEEALIELLVSVRAELRKQRNFTLADKIRDELKALGIQLEDTPQGTIWKRISV

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=511, Percent_Identity=39.5303326810176, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI303304987, Length=409, Percent_Identity=36.1858190709046, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI62240992, Length=409, Percent_Identity=36.1858190709046, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI21269877, Length=409, Percent_Identity=36.1858190709046, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI10835051, Length=409, Percent_Identity=36.1858190709046, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI1786737, Length=479, Percent_Identity=46.7640918580376, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17509795, Length=443, Percent_Identity=32.7313769751693, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17509797, Length=443, Percent_Identity=32.7313769751693, Blast_Score=221, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6324082, Length=307, Percent_Identity=41.6938110749186, Blast_Score=239, Evalue=9e-64,
Organism=Drosophila melanogaster, GI19922218, Length=506, Percent_Identity=35.9683794466403, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI19922364, Length=336, Percent_Identity=38.9880952380952, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_THEON (B6YUQ3)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306986.1
- GeneID:   7016900
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0602
- HOGENOM:   HBG327651
- OMA:   VVKIYCC
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_A
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55767; Mature: 55636

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRVYNTLTKQKEEFRPLREGEVRMYVCGPTVYDYTHLGHARTYIAFDVIRRYLEHRGY
CEEEEHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCC
TVLMVMNFTDIDDKIIRRANETGEDPKELAEKFLRYFLEDMKALKVKPADIYPRVTEHIQ
EEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DIIDFVRKLQEKGYAYEGNDGVYFEVRKFKDYGKLSGIKLEELVKGARVEPGEGKKNPED
HHHHHHHHHHHCCCEEECCCCEEEEEHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHH
FALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIA
HHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEECCCCCCEECCCCCCHHH
QSEACTGHEWVRYWLHTGFVMVSGEKMSKSLGNFVTIRELLQKYSPEVIRFFVLQKHYRS
HHHCCCHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLDYTEEGIQHAKNNLERLYNTLENIRVAMEKADVAFRWDEEEFELYETVREARKKFYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
MDDDFNTAEALKAVFEVSNAVNRYLTKVEKPKESVLRKALEFFKIVSEVFGLFEDYFKEQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGDEEALIELLVSVRAELRKQRNFTLADKIRDELKALGIQLEDTPQGTIWKRISV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCCEEECCC
>Mature Secondary Structure 
AIRVYNTLTKQKEEFRPLREGEVRMYVCGPTVYDYTHLGHARTYIAFDVIRRYLEHRGY
EEEEHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCC
TVLMVMNFTDIDDKIIRRANETGEDPKELAEKFLRYFLEDMKALKVKPADIYPRVTEHIQ
EEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DIIDFVRKLQEKGYAYEGNDGVYFEVRKFKDYGKLSGIKLEELVKGARVEPGEGKKNPED
HHHHHHHHHHHCCCEEECCCCEEEEEHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHH
FALWKKAKPGEPKWESPWGEGRPGWHIECSTMSTKYLGESFDIHGGGNDLIFPHHENEIA
HHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCEECCCCCCEECCCCCCHHH
QSEACTGHEWVRYWLHTGFVMVSGEKMSKSLGNFVTIRELLQKYSPEVIRFFVLQKHYRS
HHHCCCHHHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
PLDYTEEGIQHAKNNLERLYNTLENIRVAMEKADVAFRWDEEEFELYETVREARKKFYDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
MDDDFNTAEALKAVFEVSNAVNRYLTKVEKPKESVLRKALEFFKIVSEVFGLFEDYFKEQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGDEEALIELLVSVRAELRKQRNFTLADKIRDELKALGIQLEDTPQGTIWKRISV
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA