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The map label for this gene is metG

Identifier: 212223727

GI number: 212223727

Start: 532003

End: 534204

Strand: Reverse

Name: metG

Synonym: TON_0579

Alternate gene names: 212223727

Gene position: 534204-532003 (Counterclockwise)

Preceding gene: 212223729

Following gene: 212223720

Centisome position: 28.91

GC content: 53.09

Gene sequence:

>2202_bases
ATGGTCAGATACATGGTGACTTCCGCTCTTCCCTACGCCAACGGGCCGATTCATGCTGGACACCTTGCTGGAGCATATCT
GCCTGCGGATATATTTGTCCGCTATCTGAGGCTCAAGGGAGAGGAAGTGATTTTCATCAGTGGAACCGATGAACACGGGA
CACCAATAACCTTCAGAGCGCTCAAAGAGGGGAAAAGCCCAAGGGAGATAGTCGACTACTACCACGAGCACATCAAGACG
ACCTTTGAAAGGGCCAAGATAAGCTTCGACTACTTTGGCAGAACTGAACTGCCGGTCCACTACCGCATAAGCCAAGACTT
TTTCCTCAAGGCTCTAGAGAACGATCACCTCATTAAAAAGGTGAGCAAGCAGGCCTACTGTGAGCACGACAAGATGTTTC
TTCCGGACAGGTATGTTATAGGCACGTGCCCCTACTGCGGTGCTGAGAACCAGAGGGGAGACCAGTGTGAGGTCTGCGGC
CATCCGCTCACGCCGGAAGAGCTAATAAATCCGCGCTGCAACATCTGCGGCAATCCAATAACCTTCAGGGACTCTGCTCA
CTATTACATAAAAATGCAGGATTTCGCCGAGAAGCTTAAGGAGTGGGTCCAAAGCCAGGAGCACTGGAAGCCGAACGTCA
AGAACACAGTCCTCGGCTGGATTGAGGAGGGCCTTGAGGAGAGGGCCATAACGAGAGATTTAAACTGGGGCATTCCAGTG
CCGCTCGATGACGAGGACGTCAAAGGCAAGGTTCTCTACGTCTGGTTTGAAGCGCCGATAGGGTACATCAGCATAACCGT
CGAGCACCTTAAGAGGGAAGGCAAGGAGAATGAGTGGAAGAAGTTCTGGCTCAACCTTGACGGAGGGACAAAGGTCATCC
ACTTCATAGGCAAGGACAACATACCCTTCCACGCGATATTCTGGCCGGCTTTCCTGATGGCTTACGGCAAGTACAAGGAA
GAAGAAACCGAGGCCGAGTGGCTCCTTCCGTACGACATCCCCGCCAACGAGTACCTCAACTTGGAAGGCAAGAAGTTCTC
AACGAGCAGAAACTGGGCCATCTGGGTGCACGAGTTCCTCGACGCCTTCCCGGCTGACTACCTCCGCTATTACCTCACCG
CTATAATGCCCGAGACGAGGGACAGCGACTTCAGCTTCGCCGACTTCAAGACAAAGATTAACGAGGAGCTGGTGAACAAC
CTCGGAAACTTCGTGCACAGGGCTCTAACCTTCGTGAACCGCTACTTCGACGGCGTCGTCCCCGAAAGGGGCGAGCTTGA
TGATCTGGACAGGCAGGCCTTCGAGGAGATTGAGAAAGCTCTGAAAGAGACGGGCGAGCTGATAAGCACCTACCGCTTCA
AAGACGCTCTCAGGCGTGTAATGGAGCTGGCAATCTTCGGCAACCGCTACTTCGACTACCAGAAGCCATGGAAGACAGCC
AAGGAAAACCGCGAGAGGACGGCAACAACTGTTAATGTCTCGCTCCAGATCGTCAAGGTCCTTGGAGTGCTCCTCGAGCC
GTTCCTGCCGGATGCCAGTGAGAAGATATGGCACCTGCTCAACCTCGAGGAGACCAAGCGCTGGGAGTTCATCGAGATTC
CAGCAGGCCACAGGGTTAGGAGAGCGGAAATCCTCTTCAGAAAGGTCACTGATGAGGACATAATATTCTTCATCGTGAAC
TACATAGCCAGAGGCAACCCTGAGAGCGCAAGGCTGCTCCTTGACAAGTACTACAGGACGGACGACGTCGTTAAGGTTGC
CCTCGAGAGGTTCGGCGAGAAGCGTAAAGATGAGGCCATGGCAATCCTTAAGAGCATATACGGCGAAAGGCTTGAGAAGA
AGGCCAAAAAGGAGAAGAAAAAGGAGGGTGGAAAAGTGGAGTACGTTAAGTTCGAGGACTTCATGAAGCTCGACTTGAGG
GTTGGAAAGATTGTTGATGTGCAGGATCACCCAAACGCGGACAAGCTCTACGTGGTAAAGGTTGACCTCGGTAATGAGGT
CAGGCAGCTCGTCGCTGGACTCAAGAAGTACTACAGCAAGGACGACCTCCTCAACCGCTACGTCGTTATCATAGCCAACC
TCGAGCCCAAGAAGCTCAGGGGAATAGAGAGCCAGGGAATGCTCCTCGCTGCCGACGACGGCGAGAACGTTGCCCTGCTT
ATGCCGGACAAGGAGGTAAAGCTCGGGGCAAGAATAAGATGA

Upstream 100 bases:

>100_bases
ATAACTCAACATATTTTCGACCTTTAAAAATAACCCACCGTAATGCTTAAAATCCGCAGGTCTTATAAGGGACTAAAACG
ACGGTGTGGCGGTGGTTAAA

Downstream 100 bases:

>100_bases
GGGGAGCTCAAAGCCTGAGCTCCACCCAGTTTTCCCTCTTCTTCTTGTAGACCCTCACAAGGCCCTGCTTTTCGAGCCTC
TGGAACATTCTCCAGGCAGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVIFISGTDEHGTPITFRALKEGKSPREIVDYYHEHIKT
TFERAKISFDYFGRTELPVHYRISQDFFLKALENDHLIKKVSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCG
HPLTPEELINPRCNICGNPITFRDSAHYYIKMQDFAEKLKEWVQSQEHWKPNVKNTVLGWIEEGLEERAITRDLNWGIPV
PLDDEDVKGKVLYVWFEAPIGYISITVEHLKREGKENEWKKFWLNLDGGTKVIHFIGKDNIPFHAIFWPAFLMAYGKYKE
EETEAEWLLPYDIPANEYLNLEGKKFSTSRNWAIWVHEFLDAFPADYLRYYLTAIMPETRDSDFSFADFKTKINEELVNN
LGNFVHRALTFVNRYFDGVVPERGELDDLDRQAFEEIEKALKETGELISTYRFKDALRRVMELAIFGNRYFDYQKPWKTA
KENRERTATTVNVSLQIVKVLGVLLEPFLPDASEKIWHLLNLEETKRWEFIEIPAGHRVRRAEILFRKVTDEDIIFFIVN
YIARGNPESARLLLDKYYRTDDVVKVALERFGEKRKDEAMAILKSIYGERLEKKAKKEKKKEGGKVEYVKFEDFMKLDLR
VGKIVDVQDHPNADKLYVVKVDLGNEVRQLVAGLKKYYSKDDLLNRYVVIIANLEPKKLRGIESQGMLLAADDGENVALL
MPDKEVKLGARIR

Sequences:

>Translated_733_residues
MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVIFISGTDEHGTPITFRALKEGKSPREIVDYYHEHIKT
TFERAKISFDYFGRTELPVHYRISQDFFLKALENDHLIKKVSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCG
HPLTPEELINPRCNICGNPITFRDSAHYYIKMQDFAEKLKEWVQSQEHWKPNVKNTVLGWIEEGLEERAITRDLNWGIPV
PLDDEDVKGKVLYVWFEAPIGYISITVEHLKREGKENEWKKFWLNLDGGTKVIHFIGKDNIPFHAIFWPAFLMAYGKYKE
EETEAEWLLPYDIPANEYLNLEGKKFSTSRNWAIWVHEFLDAFPADYLRYYLTAIMPETRDSDFSFADFKTKINEELVNN
LGNFVHRALTFVNRYFDGVVPERGELDDLDRQAFEEIEKALKETGELISTYRFKDALRRVMELAIFGNRYFDYQKPWKTA
KENRERTATTVNVSLQIVKVLGVLLEPFLPDASEKIWHLLNLEETKRWEFIEIPAGHRVRRAEILFRKVTDEDIIFFIVN
YIARGNPESARLLLDKYYRTDDVVKVALERFGEKRKDEAMAILKSIYGERLEKKAKKEKKKEGGKVEYVKFEDFMKLDLR
VGKIVDVQDHPNADKLYVVKVDLGNEVRQLVAGLKKYYSKDDLLNRYVVIIANLEPKKLRGIESQGMLLAADDGENVALL
MPDKEVKLGARIR
>Mature_733_residues
MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVIFISGTDEHGTPITFRALKEGKSPREIVDYYHEHIKT
TFERAKISFDYFGRTELPVHYRISQDFFLKALENDHLIKKVSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCG
HPLTPEELINPRCNICGNPITFRDSAHYYIKMQDFAEKLKEWVQSQEHWKPNVKNTVLGWIEEGLEERAITRDLNWGIPV
PLDDEDVKGKVLYVWFEAPIGYISITVEHLKREGKENEWKKFWLNLDGGTKVIHFIGKDNIPFHAIFWPAFLMAYGKYKE
EETEAEWLLPYDIPANEYLNLEGKKFSTSRNWAIWVHEFLDAFPADYLRYYLTAIMPETRDSDFSFADFKTKINEELVNN
LGNFVHRALTFVNRYFDGVVPERGELDDLDRQAFEEIEKALKETGELISTYRFKDALRRVMELAIFGNRYFDYQKPWKTA
KENRERTATTVNVSLQIVKVLGVLLEPFLPDASEKIWHLLNLEETKRWEFIEIPAGHRVRRAEILFRKVTDEDIIFFIVN
YIARGNPESARLLLDKYYRTDDVVKVALERFGEKRKDEAMAILKSIYGERLEKKAKKEKKKEGGKVEYVKFEDFMKLDLR
VGKIVDVQDHPNADKLYVVKVDLGNEVRQLVAGLKKYYSKDDLLNRYVVIIANLEPKKLRGIESQGMLLAADDGENVALL
MPDKEVKLGARIR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=609, Percent_Identity=34.8111658456486, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=26.9439421338156, Blast_Score=175, Evalue=2e-43,
Organism=Homo sapiens, GI4507947, Length=78, Percent_Identity=53.8461538461538, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI215490011, Length=101, Percent_Identity=39.6039603960396, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI215490009, Length=101, Percent_Identity=39.6039603960396, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI45006986, Length=101, Percent_Identity=39.6039603960396, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788432, Length=738, Percent_Identity=32.520325203252, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789455, Length=109, Percent_Identity=45.8715596330275, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786861, Length=237, Percent_Identity=24.8945147679325, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=582, Percent_Identity=35.3951890034364, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI212645096, Length=537, Percent_Identity=26.8156424581006, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71992781, Length=432, Percent_Identity=24.537037037037, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=565, Percent_Identity=36.9911504424779, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321610, Length=558, Percent_Identity=23.1182795698925, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321333, Length=98, Percent_Identity=38.7755102040816, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=37.2093023255814, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21358483, Length=546, Percent_Identity=24.5421245421245, Blast_Score=151, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651924, Length=91, Percent_Identity=46.1538461538462, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357223, Length=85, Percent_Identity=42.3529411764706, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_THEON (B6YUN0)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306963.1
- GeneID:   7016877
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0579
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 85457; Mature: 85457

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVIFISGTDEHGTPITFRA
CCEEEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHH
LKEGKSPREIVDYYHEHIKTTFERAKISFDYFGRTELPVHYRISQDFFLKALENDHLIKK
HHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECHHHHHHHHCCCHHHHH
VSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCGHPLTPEELINPRCNICGNPI
HHHHHHHCCCCEECCCCEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHCCCCCCCCCCCE
TFRDSAHYYIKMQDFAEKLKEWVQSQEHWKPNVKNTVLGWIEEGLEERAITRDLNWGIPV
EEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLDDEDVKGKVLYVWFEAPIGYISITVEHLKREGKENEWKKFWLNLDGGTKVIHFIGKDN
CCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCC
IPFHAIFWPAFLMAYGKYKEEETEAEWLLPYDIPANEYLNLEGKKFSTSRNWAIWVHEFL
CCEEHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECCCCCEECCCCCCEEEHHHHH
DAFPADYLRYYLTAIMPETRDSDFSFADFKTKINEELVNNLGNFVHRALTFVNRYFDGVV
HHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PERGELDDLDRQAFEEIEKALKETGELISTYRFKDALRRVMELAIFGNRYFDYQKPWKTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
KENRERTATTVNVSLQIVKVLGVLLEPFLPDASEKIWHLLNLEETKRWEFIEIPAGHRVR
HHCCHHCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
RAEILFRKVTDEDIIFFIVNYIARGNPESARLLLDKYYRTDDVVKVALERFGEKRKDEAM
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHH
AILKSIYGERLEKKAKKEKKKEGGKVEYVKFEDFMKLDLRVGKIVDVQDHPNADKLYVVK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEEECEEEECCCCCCCCEEEEEE
VDLGNEVRQLVAGLKKYYSKDDLLNRYVVIIANLEPKKLRGIESQGMLLAADDGENVALL
ECCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEE
MPDKEVKLGARIR
ECCCCCCCCCCCC
>Mature Secondary Structure
MVRYMVTSALPYANGPIHAGHLAGAYLPADIFVRYLRLKGEEVIFISGTDEHGTPITFRA
CCEEEEECCCCCCCCCCCCCHHCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHH
LKEGKSPREIVDYYHEHIKTTFERAKISFDYFGRTELPVHYRISQDFFLKALENDHLIKK
HHCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEECHHHHHHHHCCCHHHHH
VSKQAYCEHDKMFLPDRYVIGTCPYCGAENQRGDQCEVCGHPLTPEELINPRCNICGNPI
HHHHHHHCCCCEECCCCEEEECCCCCCCCCCCCCCHHCCCCCCCHHHHCCCCCCCCCCCE
TFRDSAHYYIKMQDFAEKLKEWVQSQEHWKPNVKNTVLGWIEEGLEERAITRDLNWGIPV
EEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLDDEDVKGKVLYVWFEAPIGYISITVEHLKREGKENEWKKFWLNLDGGTKVIHFIGKDN
CCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCC
IPFHAIFWPAFLMAYGKYKEEETEAEWLLPYDIPANEYLNLEGKKFSTSRNWAIWVHEFL
CCEEHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEECCCCCEECCCCCCEEEHHHHH
DAFPADYLRYYLTAIMPETRDSDFSFADFKTKINEELVNNLGNFVHRALTFVNRYFDGVV
HHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PERGELDDLDRQAFEEIEKALKETGELISTYRFKDALRRVMELAIFGNRYFDYQKPWKTA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
KENRERTATTVNVSLQIVKVLGVLLEPFLPDASEKIWHLLNLEETKRWEFIEIPAGHRVR
HHCCHHCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHH
RAEILFRKVTDEDIIFFIVNYIARGNPESARLLLDKYYRTDDVVKVALERFGEKRKDEAM
HHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHH
AILKSIYGERLEKKAKKEKKKEGGKVEYVKFEDFMKLDLRVGKIVDVQDHPNADKLYVVK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHEEECEEEECCCCCCCCEEEEEE
VDLGNEVRQLVAGLKKYYSKDDLLNRYVVIIANLEPKKLRGIESQGMLLAADDGENVALL
ECCCHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCHHHHCCCCCCCEEEEECCCCEEEEE
MPDKEVKLGARIR
ECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA