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The map label for this gene is proS [H]

Identifier: 212223716

GI number: 212223716

Start: 521451

End: 522899

Strand: Reverse

Name: proS [H]

Synonym: TON_0568

Alternate gene names: 212223716

Gene position: 522899-521451 (Counterclockwise)

Preceding gene: 212223720

Following gene: 212223714

Centisome position: 28.3

GC content: 54.24

Gene sequence:

>1449_bases
ATGAAGGTTGAGCGCAAGAAGTGGAGCGAGGAGTTCAGCGAGTGGTACAACGAGCTGATTGAAACTGCCGGGATACAGGA
CAAGCGTTACCCTGTCAAGGGAATGAACATCTGGCTCCCGTACGGGCTTAAAATAATGCGCAACATTGAGAGGTTCATAC
ATGCCGAGATGGAAAGAACCGGCCATGATGAGGTTCTCTTCCCGGCACTCATCCCTGAAACCGAGTTCCAGAAGGAGGCA
GAACACATAGCGGGCTTTGAGGGGGAGGTCTTCTGGGTCACCCACGCCGGTCACGATCCCCTCGACATCAAGCTCATCCT
CAGGCCGACGAGCGAGACTGCAATGTACTCAATGTTCGCTCTCTGGATTCGCTCACATGCCGACCTTCCATTCAAGGTCT
ACCAGATAGTTAACACTTACCGCTACGAGACCAAGCACACGAGGCCGCTCATCCGTGTCAGGGAAATCAGCAGGTTCTTC
GAGGCCCACACGGCTCACGATAGCTTTGAGGATGCCGAGAGGCAGATAAAGGAGGATCTTGAGATATTCGACAACCTCGC
CAAGTTCCTTGCGATTCCATACATCGTCTCCAAGAGGCCCGACTGGGACAAGTTCCCGGGAGCTTACTATTCCCTCGGTG
CCGAAGTAATGATGCCCGACGGCAGAACGCTTCAGATAGGAACGATGCACAACTACAGGCAGAACTTCGCAAAGGCCTAC
GAGATACTCTATGAGACCGAAAACGGTGACCACGAGTACGTTCACCAGACGACATTCGGAATGAGCGAGAGGCTTCTCGC
AGCTGTTATAGCAATCCATGGAGACGACAGGGGAATGGTTCTGCCACCCACAATAGCTCCAATACAGGTCGTCATTGTTC
CGATTCCAAAGAAAGACGCAGGGGCAGATGTATTCACCTACGCTCGCGAGATTGCAGAGGAGCTTAGAACAGCTGGCCTG
AGGGTGCATGTAGACGAGCGCGACATAAGGCCTGGCAGAAAGTTCTACGACTGGGAGCTCAAAGGCGTGCCGCTTAGAAT
AGAGGTCGGCCCAAGAGACGTTGAAGGAAGGAAAGCAGTTCTAGCGAGACGTGACACGCTCACGAAGGAGACTGTTGAGA
GGGATAGAATAGTCGAGGCTGTCAGGAGGACCCTCGACGAAATCCACGAGAACCTCCACAAGAGGGCCGAAGAGTTCCTC
AGGACCCACATCAAGCGCGTTGACACCATCGAGGAGGCCAAGGCAGTCTTTGAGGACAGGCGCGGAATAGTTGAGATTCC
CTGGTGTGGCGAGGAGGAGTGCGGCCTGAAGATGGAGGAAGAGCTTGACGCCAAGATGCTCGGAACGCCCTATCCCGAGG
AGAAGGCCAAGGAAGGCATCGAGGGCAAGAAGTGCCCTGTCTGCGGAAGAGACGCCAAGTTCATCGCCAGGTTTGCCAGA
ACATACTGA

Upstream 100 bases:

>100_bases
AACGAACTATTAAGGTTTTTGCCCGAGGTTTGTCAGAATGTCCGCTGCCCCCCGGGAAAGCTTAAAAAGTTTTTTCGGCC
TCTCACTTTAGGTGGTGTCG

Downstream 100 bases:

>100_bases
GCTTTCCTTTCTATTCTCCTACACTTCGCTTTTGAAGCACAAGTAGAAAACAGCTGAGAAAAGGAAATATGACGAAATCA
AGCACTCTCGTTCTCGATGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKVERKKWSEEFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHAEMERTGHDEVLFPALIPETEFQKEA
EHIAGFEGEVFWVTHAGHDPLDIKLILRPTSETAMYSMFALWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFF
EAHTAHDSFEDAERQIKEDLEIFDNLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAY
EILYETENGDHEYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKDAGADVFTYAREIAEELRTAGL
RVHVDERDIRPGRKFYDWELKGVPLRIEVGPRDVEGRKAVLARRDTLTKETVERDRIVEAVRRTLDEIHENLHKRAEEFL
RTHIKRVDTIEEAKAVFEDRRGIVEIPWCGEEECGLKMEEELDAKMLGTPYPEEKAKEGIEGKKCPVCGRDAKFIARFAR
TY

Sequences:

>Translated_482_residues
MKVERKKWSEEFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHAEMERTGHDEVLFPALIPETEFQKEA
EHIAGFEGEVFWVTHAGHDPLDIKLILRPTSETAMYSMFALWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFF
EAHTAHDSFEDAERQIKEDLEIFDNLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAY
EILYETENGDHEYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKDAGADVFTYAREIAEELRTAGL
RVHVDERDIRPGRKFYDWELKGVPLRIEVGPRDVEGRKAVLARRDTLTKETVERDRIVEAVRRTLDEIHENLHKRAEEFL
RTHIKRVDTIEEAKAVFEDRRGIVEIPWCGEEECGLKMEEELDAKMLGTPYPEEKAKEGIEGKKCPVCGRDAKFIARFAR
TY
>Mature_482_residues
MKVERKKWSEEFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHAEMERTGHDEVLFPALIPETEFQKEA
EHIAGFEGEVFWVTHAGHDPLDIKLILRPTSETAMYSMFALWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFF
EAHTAHDSFEDAERQIKEDLEIFDNLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAY
EILYETENGDHEYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKDAGADVFTYAREIAEELRTAGL
RVHVDERDIRPGRKFYDWELKGVPLRIEVGPRDVEGRKAVLARRDTLTKETVERDRIVEAVRRTLDEIHENLHKRAEEFL
RTHIKRVDTIEEAKAVFEDRRGIVEIPWCGEEECGLKMEEELDAKMLGTPYPEEKAKEGIEGKKCPVCGRDAKFIARFAR
TY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=506, Percent_Identity=34.1897233201581, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1786392, Length=169, Percent_Identity=30.1775147928994, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71991342, Length=491, Percent_Identity=32.1792260692464, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71991346, Length=351, Percent_Identity=35.042735042735, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321808, Length=502, Percent_Identity=32.2709163346614, Blast_Score=266, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24649468, Length=498, Percent_Identity=33.7349397590361, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24649466, Length=498, Percent_Identity=33.7349397590361, Blast_Score=278, Evalue=7e-75,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 56184; Mature: 56184

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVERKKWSEEFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHAEMERT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GHDEVLFPALIPETEFQKEAEHIAGFEGEVFWVTHAGHDPLDIKLILRPTSETAMYSMFA
CCCCEEEHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHH
LWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFFEAHTAHDSFEDAERQIKEDL
HHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIFDNLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAY
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCCEEEEECHHHHHHHHHHHH
EILYETENGDHEYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKDA
HHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHEECCCCCEECCCCCCCEEEEEEECCCCCC
GADVFTYAREIAEELRTAGLRVHVDERDIRPGRKFYDWELKGVPLRIEVGPRDVEGRKAV
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHH
LARRDTLTKETVERDRIVEAVRRTLDEIHENLHKRAEEFLRTHIKRVDTIEEAKAVFEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGIVEIPWCGEEECGLKMEEELDAKMLGTPYPEEKAKEGIEGKKCPVCGRDAKFIARFAR
CCEEECCCCCCHHCCCCCHHHHCHHHCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHH
TY
CC
>Mature Secondary Structure
MKVERKKWSEEFSEWYNELIETAGIQDKRYPVKGMNIWLPYGLKIMRNIERFIHAEMERT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHC
GHDEVLFPALIPETEFQKEAEHIAGFEGEVFWVTHAGHDPLDIKLILRPTSETAMYSMFA
CCCCEEEHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHH
LWIRSHADLPFKVYQIVNTYRYETKHTRPLIRVREISRFFEAHTAHDSFEDAERQIKEDL
HHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EIFDNLAKFLAIPYIVSKRPDWDKFPGAYYSLGAEVMMPDGRTLQIGTMHNYRQNFAKAY
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCCEEEEECHHHHHHHHHHHH
EILYETENGDHEYVHQTTFGMSERLLAAVIAIHGDDRGMVLPPTIAPIQVVIVPIPKKDA
HHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHEECCCCCEECCCCCCCEEEEEEECCCCCC
GADVFTYAREIAEELRTAGLRVHVDERDIRPGRKFYDWELKGVPLRIEVGPRDVEGRKAV
CCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEECCEEEEEEECCCCCCCHHHH
LARRDTLTKETVERDRIVEAVRRTLDEIHENLHKRAEEFLRTHIKRVDTIEEAKAVFEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGIVEIPWCGEEECGLKMEEELDAKMLGTPYPEEKAKEGIEGKKCPVCGRDAKFIARFAR
CCEEECCCCCCHHCCCCCHHHHCHHHCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHH
TY
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA