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The map label for this gene is nuoN [H]

Identifier: 212223639

GI number: 212223639

Start: 452679

End: 454166

Strand: Reverse

Name: nuoN [H]

Synonym: TON_0492

Alternate gene names: 212223639

Gene position: 454166-452679 (Counterclockwise)

Preceding gene: 212223640

Following gene: 212223638

Centisome position: 24.58

GC content: 56.25

Gene sequence:

>1488_bases
ATGAACGGGCAGTACGCTGCTCTGCTCATAGCGTTACCCCTCATCAGCGCGTTCTTCGTCCCCGTGTTTAAGGGAATGGG
AAAGAACGCCGTCAGGTACTTCCTGATCCTAGTTACCGCCCTCCAGACTGGAATAGCCGCATGGGTCTTCAAGGAGGTCT
ATACTACTGGCCAGCCAATAATAGTCATCGCCGGCGGCTGGAAGCCACCCGTTGGAATCAACCTCTACCTCGGCCATTTC
GCGGCGCTCTTCGTACTCATAATAGCAGTCGTTAGCCTCTTCACTTCGATCTTCAGCATCAGCGCGGTCAAAACCGAACC
CATCGACAAGTATGCCATGCTCTTCCTCCTGCTGATGCTTGGTGCCACCGGAATGATAGCGACCGGTGATATATTCAACC
TCTTCGTCTTCATGGAGATAACGGCAATAACTGCCTACGCCCTCACGGCCTACAACAAGACAGGTGAATCGGCCGAAGCG
TCAATGAAGTACATCATCCTCGGTGGAATCGGTTCAAGCTTCTTCCTCATCGGTGTCGCTTTAATCTACGGCTCCCTCGG
AACCCTCAACATGGCCCAGATAGCCCAGCTCGCAGCAACTATGGATCCCACCGTAGCCCAGGTCGGCCTCGCGCTCATAA
TTCTTGGTCTGGCCGTCGAAGCTGAGCTCTTCCCGCTCAACGCCTGGGCGCCCGACGCTTATCAGGCAGCACCGCACCCA
ATCACCGTCATGTTCTCCGCCTTTGTCGTCAAGGCTGCCCTCTACGCCATGGCGAGGATTATCTACCTCATGCAGGTCGT
CGAGAGCTGGGGCTCAGTGCTCAGGCTTCTCATCGTAATGGCCGCCCTCACCGTCGTCGTTGCCGAGCTGGCAGCGCTTA
GGCAGAAGAACGTCAAGAGGATGATAGCCTACTCCAGTATCGCCCAGATAGGCTTCATAGCACTCGCCTTCGCCCTCGGC
ACTCAGGCCGGCGTTGACGCTGGTGTCTTCCACATGATCAACCACGCAATAGTCAAAGTTCTCCTGTTCCTCGCCGTCGG
CTACGTGGCGATAACCCTCGGCGGTGCAGAGCTGGAGAAGTTCCAGGGACTCGGCAGGAGGATGCCGCTCACGGCCTTCG
CTATCTCAGTTGGTGCAGTAGCCACCATCGGAATACCGCTCTTCAACGTCTTCTGGAGCAAGATCAGAATAATCCTTGCG
ACCCTGAACGTCGGCTACACCTGGGCGGCAGTACTTGTACTTTCAGCGAGCGTCGTGGAGGCGGTCTATTACTTCAGGCT
GCTCCACGCCATATGGTTCGAAGGAGAGGGAGAGAGAATCAGCGAGAGCATGGTCATTGGCCTCATGATGTTCTTCCTCG
CGGCTTTGGTGGTCGTCATAGGAGTCTACCCGGACTACGTTTGGGAGCTCGCCAGGAAGGCTGGGGAGGACATCTTCAAC
GTGGCCCAGTACGTTAAGAACGTTCCACTGATGGGGGTGGGAGCATGA

Upstream 100 bases:

>100_bases
TTATTGGAGTTGCCACCACCGCCATGGCCCTTAGCGTTGCCATACTGATTTACGAGAAGTACGGAACCCTTAACGTTGAA
GAGATAAGGAGGTTGAGAGG

Downstream 100 bases:

>100_bases
TTAACGAACTCATCGTTATCATTTTCGCGCCTCTGATTGCTGGCGTTATCGCCTGGGCGCTTGACATCAAAGTTATTAGA
GAGCTGCTCGGCGTTGCCGG

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNGQYAALLIALPLISAFFVPVFKGMGKNAVRYFLILVTALQTGIAAWVFKEVYTTGQPIIVIAGGWKPPVGINLYLGHF
AALFVLIIAVVSLFTSIFSISAVKTEPIDKYAMLFLLLMLGATGMIATGDIFNLFVFMEITAITAYALTAYNKTGESAEA
SMKYIILGGIGSSFFLIGVALIYGSLGTLNMAQIAQLAATMDPTVAQVGLALIILGLAVEAELFPLNAWAPDAYQAAPHP
ITVMFSAFVVKAALYAMARIIYLMQVVESWGSVLRLLIVMAALTVVVAELAALRQKNVKRMIAYSSIAQIGFIALAFALG
TQAGVDAGVFHMINHAIVKVLLFLAVGYVAITLGGAELEKFQGLGRRMPLTAFAISVGAVATIGIPLFNVFWSKIRIILA
TLNVGYTWAAVLVLSASVVEAVYYFRLLHAIWFEGEGERISESMVIGLMMFFLAALVVVIGVYPDYVWELARKAGEDIFN
VAQYVKNVPLMGVGA

Sequences:

>Translated_495_residues
MNGQYAALLIALPLISAFFVPVFKGMGKNAVRYFLILVTALQTGIAAWVFKEVYTTGQPIIVIAGGWKPPVGINLYLGHF
AALFVLIIAVVSLFTSIFSISAVKTEPIDKYAMLFLLLMLGATGMIATGDIFNLFVFMEITAITAYALTAYNKTGESAEA
SMKYIILGGIGSSFFLIGVALIYGSLGTLNMAQIAQLAATMDPTVAQVGLALIILGLAVEAELFPLNAWAPDAYQAAPHP
ITVMFSAFVVKAALYAMARIIYLMQVVESWGSVLRLLIVMAALTVVVAELAALRQKNVKRMIAYSSIAQIGFIALAFALG
TQAGVDAGVFHMINHAIVKVLLFLAVGYVAITLGGAELEKFQGLGRRMPLTAFAISVGAVATIGIPLFNVFWSKIRIILA
TLNVGYTWAAVLVLSASVVEAVYYFRLLHAIWFEGEGERISESMVIGLMMFFLAALVVVIGVYPDYVWELARKAGEDIFN
VAQYVKNVPLMGVGA
>Mature_495_residues
MNGQYAALLIALPLISAFFVPVFKGMGKNAVRYFLILVTALQTGIAAWVFKEVYTTGQPIIVIAGGWKPPVGINLYLGHF
AALFVLIIAVVSLFTSIFSISAVKTEPIDKYAMLFLLLMLGATGMIATGDIFNLFVFMEITAITAYALTAYNKTGESAEA
SMKYIILGGIGSSFFLIGVALIYGSLGTLNMAQIAQLAATMDPTVAQVGLALIILGLAVEAELFPLNAWAPDAYQAAPHP
ITVMFSAFVVKAALYAMARIIYLMQVVESWGSVLRLLIVMAALTVVVAELAALRQKNVKRMIAYSSIAQIGFIALAFALG
TQAGVDAGVFHMINHAIVKVLLFLAVGYVAITLGGAELEKFQGLGRRMPLTAFAISVGAVATIGIPLFNVFWSKIRIILA
TLNVGYTWAAVLVLSASVVEAVYYFRLLHAIWFEGEGERISESMVIGLMMFFLAALVVVIGVYPDYVWELARKAGEDIFN
VAQYVKNVPLMGVGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=367, Percent_Identity=24.5231607629428, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI251831116, Length=327, Percent_Identity=22.0183486238532, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788831, Length=380, Percent_Identity=30.2631578947368, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI145693160, Length=411, Percent_Identity=26.2773722627737, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1788614, Length=318, Percent_Identity=32.3899371069182, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788827, Length=396, Percent_Identity=25.2525252525253, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788829, Length=334, Percent_Identity=28.4431137724551, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI2367154, Length=321, Percent_Identity=26.4797507788162, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1788613, Length=443, Percent_Identity=24.6049661399549, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53192; Mature: 53192

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGQYAALLIALPLISAFFVPVFKGMGKNAVRYFLILVTALQTGIAAWVFKEVYTTGQPI
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IVIAGGWKPPVGINLYLGHFAALFVLIIAVVSLFTSIFSISAVKTEPIDKYAMLFLLLML
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GATGMIATGDIFNLFVFMEITAITAYALTAYNKTGESAEASMKYIILGGIGSSFFLIGVA
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHH
LIYGSLGTLNMAQIAQLAATMDPTVAQVGLALIILGLAVEAELFPLNAWAPDAYQAAPHP
HHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCC
ITVMFSAFVVKAALYAMARIIYLMQVVESWGSVLRLLIVMAALTVVVAELAALRQKNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIAYSSIAQIGFIALAFALGTQAGVDAGVFHMINHAIVKVLLFLAVGYVAITLGGAELEK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FQGLGRRMPLTAFAISVGAVATIGIPLFNVFWSKIRIILATLNVGYTWAAVLVLSASVVE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AVYYFRLLHAIWFEGEGERISESMVIGLMMFFLAALVVVIGVYPDYVWELARKAGEDIFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHH
VAQYVKNVPLMGVGA
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MNGQYAALLIALPLISAFFVPVFKGMGKNAVRYFLILVTALQTGIAAWVFKEVYTTGQPI
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IVIAGGWKPPVGINLYLGHFAALFVLIIAVVSLFTSIFSISAVKTEPIDKYAMLFLLLML
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GATGMIATGDIFNLFVFMEITAITAYALTAYNKTGESAEASMKYIILGGIGSSFFLIGVA
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHH
LIYGSLGTLNMAQIAQLAATMDPTVAQVGLALIILGLAVEAELFPLNAWAPDAYQAAPHP
HHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCC
ITVMFSAFVVKAALYAMARIIYLMQVVESWGSVLRLLIVMAALTVVVAELAALRQKNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIAYSSIAQIGFIALAFALGTQAGVDAGVFHMINHAIVKVLLFLAVGYVAITLGGAELEK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FQGLGRRMPLTAFAISVGAVATIGIPLFNVFWSKIRIILATLNVGYTWAAVLVLSASVVE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AVYYFRLLHAIWFEGEGERISESMVIGLMMFFLAALVVVIGVYPDYVWELARKAGEDIFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHH
VAQYVKNVPLMGVGA
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA