Click here to switch to the map view.

The map label for this gene is mrpD [H]

Identifier: 212223638

GI number: 212223638

Start: 450820

End: 452682

Strand: Reverse

Name: mrpD [H]

Synonym: TON_0491

Alternate gene names: 212223638

Gene position: 452682-450820 (Counterclockwise)

Preceding gene: 212223639

Following gene: 212223637

Centisome position: 24.5

GC content: 53.19

Gene sequence:

>1863_bases
ATGATTAACGAACTCATCGTTATCATTTTCGCGCCTCTGATTGCTGGCGTTATCGCCTGGGCGCTTGACATCAAAGTTAT
TAGAGAGCTGCTCGGCGTTGCCGGTGCAGCCCTTCCCCTAGCTTACCTCATCAAGCTCTATCCAACCGTTGATGCCGAAG
GAATGATACAGCACACGCTGAACTTTGCCGGCTTCACCCTTGAGTTCACATTAACCCACATGAGCTGGATATTCGCTATG
ATAGCCGGTGTTGTTGGCCTTGCCGCAGTGCTTGGAATGGTCTCAACCTCAAAGAACGGCTACGAGTGGCTGTTTGCCCT
GATGAGCCTCACCGGCGTCTACGGCATCTTCATAGCCTACGACCTGTTAGGGTTCTTCATCTTCTGGGAAATCATGACCT
TCGGAAGCTTCATGATGGTCCTCAAGTACAACAGGGAGGCCTCACTCAAGTACTTCGTGCTCAGCGTTATCGGAGCCTAC
GCGATGCTCATAGCGATAGGCATAATCTACGCCAAAGTCGGCTCCTTCAGCTTTATAGAAGTTTACAGAGCGTTCTCACA
GGACGCGGCACTGGCGGCTGTCGGCTCATCTACCATCTTTAGCAAGGGCGAAATTGCGTTAATCTTCGGTCTCTTCCTGG
TCGCCTTTGGCGTTAAGGCCGGGATGTTCCCGCTTCACGTGTGGGCGCCCGATGCCTACAGCGAGACCAACCAGAGCTAC
ACCGCGATGTTCAGCGGCGTCCTCAGCAAGACTGGCGTTTACGGATTTATCCTCATCTACATGCTCATAGGCTACCGCCT
GCTCTACGAATTCGGAACGATTAGAACCGTCCCCAAGTTCGGCTACATCATAGCATTTCTCGGTGGTCTAACCATTATCG
TCGGCGGCATCCTCGCGGCGCTCCAGGAGGACATCAGGAAGCTCTTCGCCTACTCCAGTATAAGCCAGATAGGTTACATC
CTAGTGGGCATAGGTGTTGGGACTGCATTGAGCATCGAAGCCGCCATCTACCACGCCATAAGCCACGCCCTCTTCAAGGG
ACTGTTCTTCCTCATAGTGGCCACCATAATCTACCGCACCGGGAAGACGGAGTTCAAAGACATGGGCGGACTGGCAGAGA
AGATGCCAGTAACATTTGCGATGGCCTTTGTAGCAATACTCAGCCTCGCTGGAATTCCGCCGATGGTCGGCTTCGCCAGC
AAGTGGCTAATCTTTGAGTCGGTCATAAGCCAGAACATGCCAATACTCGGTGCGATGGTGTTCTTTGGAAGTGCCGTAGG
TTTCGTCTACCTCATCAGGTTCACCTACGCAGTCTGGTTCGGTCAGAGGCCAACCGACCTTGATGACACCAAGGACGCTC
CATTGCCCCTTGCCATAGGCATGGGCATACTTGCGGTGCTCAACGTCGTCTTCGGTGTCGCTCCAGGTCTTGTGGCCCAG
GAGTTAAACAAGATATTCGGCAGAGAGGTCATCGGTGGAACTATATGGGAGCTGAACCTCGGCTTCGGTAAGTACAACGG
CCTCCTCCTCACCGTCTGGTTTGTGGTCGGCATGCTCATTGCGGCCATAGTCTACTTCATGGGGGCAGGCGTGAGAAAAG
TCCCCGTTACGGACACCTACCAGTCAGGAAACCCAGTAACGATGGAGTACAACCTCACCATCAGGAGGAACTTCTTCCTT
CCGCTCAAGGAGGCCCTGTCCTTCTGGCTCAGAATGAGCTTTGACAGGTTCTACAAGGACGTTGGAAAGACTGTTGAGGA
CTTCGCCGAGACCATGAGGAACTACGTCTACAATGGAAACGTCCAGAGCTATGCCTGGTATCTTGCCATAGTCCTGTTAA
TCCTCGCTATGTGGGGGGTGTGA

Upstream 100 bases:

>100_bases
TCTACCCGGACTACGTTTGGGAGCTCGCCAGGAAGGCTGGGGAGGACATCTTCAACGTGGCCCAGTACGTTAAGAACGTT
CCACTGATGGGGGTGGGAGC

Downstream 100 bases:

>100_bases
AGAATGCTTGAGGTAGCGCTGAAGGCTTTATTCCTCCTAATTTATGCGACCTTCGTCGGGTTCATGTTCATGGGAATAAT
CAGGAAAGTGAGCGCAAGGA

Product: NADH dehydrogenase subunit M

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MINELIVIIFAPLIAGVIAWALDIKVIRELLGVAGAALPLAYLIKLYPTVDAEGMIQHTLNFAGFTLEFTLTHMSWIFAM
IAGVVGLAAVLGMVSTSKNGYEWLFALMSLTGVYGIFIAYDLLGFFIFWEIMTFGSFMMVLKYNREASLKYFVLSVIGAY
AMLIAIGIIYAKVGSFSFIEVYRAFSQDAALAAVGSSTIFSKGEIALIFGLFLVAFGVKAGMFPLHVWAPDAYSETNQSY
TAMFSGVLSKTGVYGFILIYMLIGYRLLYEFGTIRTVPKFGYIIAFLGGLTIIVGGILAALQEDIRKLFAYSSISQIGYI
LVGIGVGTALSIEAAIYHAISHALFKGLFFLIVATIIYRTGKTEFKDMGGLAEKMPVTFAMAFVAILSLAGIPPMVGFAS
KWLIFESVISQNMPILGAMVFFGSAVGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAIGMGILAVLNVVFGVAPGLVAQ
ELNKIFGREVIGGTIWELNLGFGKYNGLLLTVWFVVGMLIAAIVYFMGAGVRKVPVTDTYQSGNPVTMEYNLTIRRNFFL
PLKEALSFWLRMSFDRFYKDVGKTVEDFAETMRNYVYNGNVQSYAWYLAIVLLILAMWGV

Sequences:

>Translated_620_residues
MINELIVIIFAPLIAGVIAWALDIKVIRELLGVAGAALPLAYLIKLYPTVDAEGMIQHTLNFAGFTLEFTLTHMSWIFAM
IAGVVGLAAVLGMVSTSKNGYEWLFALMSLTGVYGIFIAYDLLGFFIFWEIMTFGSFMMVLKYNREASLKYFVLSVIGAY
AMLIAIGIIYAKVGSFSFIEVYRAFSQDAALAAVGSSTIFSKGEIALIFGLFLVAFGVKAGMFPLHVWAPDAYSETNQSY
TAMFSGVLSKTGVYGFILIYMLIGYRLLYEFGTIRTVPKFGYIIAFLGGLTIIVGGILAALQEDIRKLFAYSSISQIGYI
LVGIGVGTALSIEAAIYHAISHALFKGLFFLIVATIIYRTGKTEFKDMGGLAEKMPVTFAMAFVAILSLAGIPPMVGFAS
KWLIFESVISQNMPILGAMVFFGSAVGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAIGMGILAVLNVVFGVAPGLVAQ
ELNKIFGREVIGGTIWELNLGFGKYNGLLLTVWFVVGMLIAAIVYFMGAGVRKVPVTDTYQSGNPVTMEYNLTIRRNFFL
PLKEALSFWLRMSFDRFYKDVGKTVEDFAETMRNYVYNGNVQSYAWYLAIVLLILAMWGV
>Mature_620_residues
MINELIVIIFAPLIAGVIAWALDIKVIRELLGVAGAALPLAYLIKLYPTVDAEGMIQHTLNFAGFTLEFTLTHMSWIFAM
IAGVVGLAAVLGMVSTSKNGYEWLFALMSLTGVYGIFIAYDLLGFFIFWEIMTFGSFMMVLKYNREASLKYFVLSVIGAY
AMLIAIGIIYAKVGSFSFIEVYRAFSQDAALAAVGSSTIFSKGEIALIFGLFLVAFGVKAGMFPLHVWAPDAYSETNQSY
TAMFSGVLSKTGVYGFILIYMLIGYRLLYEFGTIRTVPKFGYIIAFLGGLTIIVGGILAALQEDIRKLFAYSSISQIGYI
LVGIGVGTALSIEAAIYHAISHALFKGLFFLIVATIIYRTGKTEFKDMGGLAEKMPVTFAMAFVAILSLAGIPPMVGFAS
KWLIFESVISQNMPILGAMVFFGSAVGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAIGMGILAVLNVVFGVAPGLVAQ
ELNKIFGREVIGGTIWELNLGFGKYNGLLLTVWFVVGMLIAAIVYFMGAGVRKVPVTDTYQSGNPVTMEYNLTIRRNFFL
PLKEALSFWLRMSFDRFYKDVGKTVEDFAETMRNYVYNGNVQSYAWYLAIVLLILAMWGV

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=190, Percent_Identity=30, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI251831116, Length=272, Percent_Identity=24.2647058823529, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1788827, Length=389, Percent_Identity=27.2493573264781, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788613, Length=474, Percent_Identity=26.1603375527426, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI2367154, Length=379, Percent_Identity=27.9683377308707, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788831, Length=287, Percent_Identity=27.5261324041812, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788829, Length=369, Percent_Identity=25.4742547425474, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788614, Length=490, Percent_Identity=23.8775510204082, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI145693160, Length=320, Percent_Identity=30, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 68189; Mature: 68189

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINELIVIIFAPLIAGVIAWALDIKVIRELLGVAGAALPLAYLIKLYPTVDAEGMIQHTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
NFAGFTLEFTLTHMSWIFAMIAGVVGLAAVLGMVSTSKNGYEWLFALMSLTGVYGIFIAY
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DLLGFFIFWEIMTFGSFMMVLKYNREASLKYFVLSVIGAYAMLIAIGIIYAKVGSFSFIE
HHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VYRAFSQDAALAAVGSSTIFSKGEIALIFGLFLVAFGVKAGMFPLHVWAPDAYSETNQSY
HHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCHH
TAMFSGVLSKTGVYGFILIYMLIGYRLLYEFGTIRTVPKFGYIIAFLGGLTIIVGGILAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQEDIRKLFAYSSISQIGYILVGIGVGTALSIEAAIYHAISHALFKGLFFLIVATIIYRT
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKTEFKDMGGLAEKMPVTFAMAFVAILSLAGIPPMVGFASKWLIFESVISQNMPILGAMV
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
FFGSAVGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAIGMGILAVLNVVFGVAPGLVAQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ELNKIFGREVIGGTIWELNLGFGKYNGLLLTVWFVVGMLIAAIVYFMGAGVRKVPVTDTY
HHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
QSGNPVTMEYNLTIRRNFFLPLKEALSFWLRMSFDRFYKDVGKTVEDFAETMRNYVYNGN
CCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
VQSYAWYLAIVLLILAMWGV
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINELIVIIFAPLIAGVIAWALDIKVIRELLGVAGAALPLAYLIKLYPTVDAEGMIQHTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
NFAGFTLEFTLTHMSWIFAMIAGVVGLAAVLGMVSTSKNGYEWLFALMSLTGVYGIFIAY
HHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DLLGFFIFWEIMTFGSFMMVLKYNREASLKYFVLSVIGAYAMLIAIGIIYAKVGSFSFIE
HHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VYRAFSQDAALAAVGSSTIFSKGEIALIFGLFLVAFGVKAGMFPLHVWAPDAYSETNQSY
HHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCHH
TAMFSGVLSKTGVYGFILIYMLIGYRLLYEFGTIRTVPKFGYIIAFLGGLTIIVGGILAA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQEDIRKLFAYSSISQIGYILVGIGVGTALSIEAAIYHAISHALFKGLFFLIVATIIYRT
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKTEFKDMGGLAEKMPVTFAMAFVAILSLAGIPPMVGFASKWLIFESVISQNMPILGAMV
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
FFGSAVGFVYLIRFTYAVWFGQRPTDLDDTKDAPLPLAIGMGILAVLNVVFGVAPGLVAQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
ELNKIFGREVIGGTIWELNLGFGKYNGLLLTVWFVVGMLIAAIVYFMGAGVRKVPVTDTY
HHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
QSGNPVTMEYNLTIRRNFFLPLKEALSFWLRMSFDRFYKDVGKTVEDFAETMRNYVYNGN
CCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
VQSYAWYLAIVLLILAMWGV
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]