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The map label for this gene is argF [H]

Identifier: 212223581

GI number: 212223581

Start: 406293

End: 407240

Strand: Reverse

Name: argF [H]

Synonym: TON_0435

Alternate gene names: 212223581

Gene position: 407240-406293 (Counterclockwise)

Preceding gene: 212223587

Following gene: 212223580

Centisome position: 22.04

GC content: 57.81

Gene sequence:

>948_bases
ATGGTGGTTAGCCTAGCCGGAAGAGACCTTCTCTGCCTCCAGGACTTTACGAGGGAGGAGATTGAGACCATTCTCAAGAC
GGCCGAAATGATGAAGATCTGGAACAAGATTGGAAAGCCGCACCGCGTTCTCGAGGGCAAAACTCTCGCTATGATATTCC
AGAAGCCCTCGACCAGGACGAGGATTTCCTTCGAGGTTGGAATCTACCAGCTCGGCGGTTACGGTCTCTACCTCAACGCC
AACGACCTCCAGCTGAGGAGAGGCGAGACCATAGCCGACACCGCGCGCGTTCTCAGCCGCTATGTGGATGGAATAATGGC
TCGTGTTTTCGACCACCAGGACGTCGTTGACCTCGCCAAGTATGCCGACGTTCCGGTCATCAACGGTCTGAGCGACTTCT
CCCACCCGTGCCAGGCTCTCGCCGACTACCAGACCATACTCGAGAAGAAGGGCAGGATTCAGGGGCTTAAGGTCGTCTAC
GTCGGCGACGGAAACAACGTCGCCCACTCCCTCATGATAGCCGGAACCAAGCTCGGTGCCCACGTCGTGGTTGCTACTCC
AGAAGGCTATGAGCCTGACAAGCGCGTAATCAAGTGGGCCGAGCAGAACGCGGCCGAGAGCGGCGGCTCGTTTGAGCTCC
TCCACGACCCTGTCAAGGCCGTTAAGGACGCCGACGTTATCTACACTGACGTCTGGGCTTCAATGGGTCAGGAGGCCGAG
GCTGAGGAGAGGAGGAAGATATTCAAGCCCTTCCAGGTGAACAAGGACCTCGTCAAGCACGCCAAGCCGGACTACATCTT
CATGCACTGCCTCCCGGCCCACAGGGGCGATGAGGTTACTGACGACGTCATCGACAGCCCGAACAGCGTCGTCTTCGACG
AGGCCGAGAACAGGCTCCACGCCCAGAAGGCGGTTATGGCTCTGGTTATGGGTGGAATTAAGGTCTGA

Upstream 100 bases:

>100_bases
AAGCTCCAGAGCGTTAATGCTCAAAAGATTTCAGCAGGAAGCAGATTTATTCGACGAAAGGGTTATTAAAACCGAGCGCC
AGCTTTAAGCGGTGATGCAC

Downstream 100 bases:

>100_bases
GCTTTCTTTTCCCTATTATCTTCGAAAAAGGGCTAAAAGTTTAAAATTCTCCGCTCAAACTCTATTCATGCTAGAAAGAC
TCTTCAGCCTCGGCTACTCC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MVVSLAGRDLLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRTRISFEVGIYQLGGYGLYLNA
NDLQLRRGETIADTARVLSRYVDGIMARVFDHQDVVDLAKYADVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKVVY
VGDGNNVAHSLMIAGTKLGAHVVVATPEGYEPDKRVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAE
AEERRKIFKPFQVNKDLVKHAKPDYIFMHCLPAHRGDEVTDDVIDSPNSVVFDEAENRLHAQKAVMALVMGGIKV

Sequences:

>Translated_315_residues
MVVSLAGRDLLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRTRISFEVGIYQLGGYGLYLNA
NDLQLRRGETIADTARVLSRYVDGIMARVFDHQDVVDLAKYADVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKVVY
VGDGNNVAHSLMIAGTKLGAHVVVATPEGYEPDKRVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAE
AEERRKIFKPFQVNKDLVKHAKPDYIFMHCLPAHRGDEVTDDVIDSPNSVVFDEAENRLHAQKAVMALVMGGIKV
>Mature_315_residues
MVVSLAGRDLLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRTRISFEVGIYQLGGYGLYLNA
NDLQLRRGETIADTARVLSRYVDGIMARVFDHQDVVDLAKYADVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKVVY
VGDGNNVAHSLMIAGTKLGAHVVVATPEGYEPDKRVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAE
AEERRKIFKPFQVNKDLVKHAKPDYIFMHCLPAHRGDEVTDDVIDSPNSVVFDEAENRLHAQKAVMALVMGGIKV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=46.6019417475728, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=28.9808917197452, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=40.0621118012422, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=39.0909090909091, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=29.9065420560748, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994908, Length=342, Percent_Identity=25.7309941520468, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=26.219512195122, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322373, Length=320, Percent_Identity=39.375, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=28.7539936102236, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=27.8481012658228, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35081; Mature: 35081

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSLAGRDLLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRT
CEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCE
RISFEVGIYQLGGYGLYLNANDLQLRRGETIADTARVLSRYVDGIMARVFDHQDVVDLAK
EEEEEEEEEEECCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YADVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKVVYVGDGNNVAHSLMIAGTKLGA
HCCCCEECCCHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHEEEEECCCC
HVVVATPEGYEPDKRVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAE
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
AEERRKIFKPFQVNKDLVKHAKPDYIFMHCLPAHRGDEVTDDVIDSPNSVVFDEAENRLH
HHHHHHHCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCEEECHHHHHHH
AQKAVMALVMGGIKV
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MVVSLAGRDLLCLQDFTREEIETILKTAEMMKIWNKIGKPHRVLEGKTLAMIFQKPSTRT
CEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEECCCCCE
RISFEVGIYQLGGYGLYLNANDLQLRRGETIADTARVLSRYVDGIMARVFDHQDVVDLAK
EEEEEEEEEEECCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
YADVPVINGLSDFSHPCQALADYQTILEKKGRIQGLKVVYVGDGNNVAHSLMIAGTKLGA
HCCCCEECCCHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHEEEEECCCC
HVVVATPEGYEPDKRVIKWAEQNAAESGGSFELLHDPVKAVKDADVIYTDVWASMGQEAE
EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
AEERRKIFKPFQVNKDLVKHAKPDYIFMHCLPAHRGDEVTDDVIDSPNSVVFDEAENRLH
HHHHHHHCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHCCCCCEEECHHHHHHH
AQKAVMALVMGGIKV
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA