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The map label for this gene is yebU [C]

Identifier: 212223580

GI number: 212223580

Start: 405296

End: 406411

Strand: Reverse

Name: yebU [C]

Synonym: TON_0434

Alternate gene names: 212223580

Gene position: 406411-405296 (Counterclockwise)

Preceding gene: 212223581

Following gene: 212223579

Centisome position: 22.0

GC content: 53.23

Gene sequence:

>1116_bases
ATGAGGTTACTGACGACGTCATCGACAGCCCGAACAGCGTCGTCTTCGACGAGGCCGAGAACAGGCTCCACGCCCAGAAG
GCGGTTATGGCTCTGGTTATGGGTGGAATTAAGGTCTGAGCTTTCTTTTCCCTATTATCTTCGAAAAAGGGCTAAAAGTT
TAAAATTCTCCGCTCAAACTCTATTCATGCTAGAAAGACTCTTCAGCCTCGGCTACTCCAAGACCTTCGCGGAGCGCTAT
TACCAGCTCTGGGGTGAGAGGGCCCTAGCAATAGCGGAGGCCATGGAAAAGCCCCTTCCAAGATGCTTCCGCGTGAATAC
TCTTCGCGTTGAGATTCCAACGCTCACGAAGCTCCTCAACAAGAAGGGCTTTCAGTTCAGGCGCGTTCCGTGGGCTCGGG
AAGGCTTCTGCCTCACGCGCGAGCCTTTCTCGATAACCTCTACGCCTGAATACCTGAGCGGCCTCCTCTACATCCAGGAG
GCCAGCTCGATGTATCCTCCCGTGGCTTTAGACCCGAAGCCCGGAGAGATTGTAGCAGACATGGCCGCCGCCCCCGGTGG
AAAGACAAGCTATCTCGCTCAACTCATGAAGAACGAGGGTATCATTTACGCCTTTGATGTTGGAGAAGAGCGGTTAAAGG
AGACACGCCTGAACCTCTCTCGCCTCGGGATCACCAACACGATACTCATTCACAAGTCCTCACTCCACATGGGTGAGCTT
GGCATCGAGTTCGACAAAATCCTTCTCGATGCCCCATGCACAGGCTCCGGCACAATCCACAAGAACCCAGAGCGGAAGTC
CAACAGAACTATGGAAGATATAAAGTTCTGTCAGGGACTGCAGATGCAGATGATTAAGGTCGCCCTTGAAAACCTCAAGC
CGGGTGGAATACTCGTCTACTCAACCTGTTCCCTCGAGCCGGAAGAGAACGAGTTTGTGATCCAGTGGACTCTCGACAGC
TTTGACGTCGAACTTCTACCTCTCAGATACGGGGAGCCTGCCCTGATCAAGCCCTTCGGAGTCAAGCTGAGCGGAGAGAT
AAAGAAGGCAAGGCGCTTTTACCCTGACAGACATGGAACCAGCGGCTTCTTCGTGGCAAAGCTCAGGAAGCTTTAG

Upstream 100 bases:

>100_bases
AGGAGGAAGATATTCAAGCCCTTCCAGGTGAACAAGGACCTCGTCAAGCACGCCAAGCCGGACTACATCTTCATGCACTG
CCTCCCGGCCCACAGGGGCG

Downstream 100 bases:

>100_bases
GGAAAGTTTTTATCAGATATAGTCATAGAGATAAAGTAGCTAAACTAGGAGGGGTCATTGATGAAATTGAGAATCGATAG
GTTTTTGGAATCTCCCACTC

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRLLTTSSTARTASSSTRPRTGSTPRRRLWLWLWVELRSELSFPYYLRKRAKSLKFSAQTLFMLERLFSLGYSKTFAERY
YQLWGERALAIAEAMEKPLPRCFRVNTLRVEIPTLTKLLNKKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQE
ASSMYPPVALDPKPGEIVADMAAAPGGKTSYLAQLMKNEGIIYAFDVGEERLKETRLNLSRLGITNTILIHKSSLHMGEL
GIEFDKILLDAPCTGSGTIHKNPERKSNRTMEDIKFCQGLQMQMIKVALENLKPGGILVYSTCSLEPEENEFVIQWTLDS
FDVELLPLRYGEPALIKPFGVKLSGEIKKARRFYPDRHGTSGFFVAKLRKL

Sequences:

>Translated_371_residues
MRLLTTSSTARTASSSTRPRTGSTPRRRLWLWLWVELRSELSFPYYLRKRAKSLKFSAQTLFMLERLFSLGYSKTFAERY
YQLWGERALAIAEAMEKPLPRCFRVNTLRVEIPTLTKLLNKKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQE
ASSMYPPVALDPKPGEIVADMAAAPGGKTSYLAQLMKNEGIIYAFDVGEERLKETRLNLSRLGITNTILIHKSSLHMGEL
GIEFDKILLDAPCTGSGTIHKNPERKSNRTMEDIKFCQGLQMQMIKVALENLKPGGILVYSTCSLEPEENEFVIQWTLDS
FDVELLPLRYGEPALIKPFGVKLSGEIKKARRFYPDRHGTSGFFVAKLRKL
>Mature_371_residues
MRLLTTSSTARTASSSTRPRTGSTPRRRLWLWLWVELRSELSFPYYLRKRAKSLKFSAQTLFMLERLFSLGYSKTFAERY
YQLWGERALAIAEAMEKPLPRCFRVNTLRVEIPTLTKLLNKKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQE
ASSMYPPVALDPKPGEIVADMAAAPGGKTSYLAQLMKNEGIIYAFDVGEERLKETRLNLSRLGITNTILIHKSSLHMGEL
GIEFDKILLDAPCTGSGTIHKNPERKSNRTMEDIKFCQGLQMQMIKVALENLKPGGILVYSTCSLEPEENEFVIQWTLDS
FDVELLPLRYGEPALIKPFGVKLSGEIKKARRFYPDRHGTSGFFVAKLRKL

Specific function: May act as RNA methyltransferase [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=286, Percent_Identity=41.2587412587413, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI76150623, Length=286, Percent_Identity=41.2587412587413, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI270288816, Length=297, Percent_Identity=31.3131313131313, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI8922322, Length=297, Percent_Identity=31.3131313131313, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI23199998, Length=297, Percent_Identity=31.3131313131313, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI270288818, Length=297, Percent_Identity=31.3131313131313, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI32698918, Length=217, Percent_Identity=33.1797235023041, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI301336155, Length=261, Percent_Identity=30.2681992337165, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI39995082, Length=288, Percent_Identity=29.5138888888889, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI11545785, Length=260, Percent_Identity=30, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI87081985, Length=285, Percent_Identity=40.3508771929825, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI2367212, Length=300, Percent_Identity=31.6666666666667, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536757, Length=311, Percent_Identity=36.0128617363344, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI25143471, Length=280, Percent_Identity=28.9285714285714, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71998419, Length=219, Percent_Identity=31.9634703196347, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534021, Length=224, Percent_Identity=31.25, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324268, Length=311, Percent_Identity=38.5852090032154, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319447, Length=272, Percent_Identity=29.7794117647059, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324306, Length=204, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI22024126, Length=286, Percent_Identity=36.3636363636364, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21355201, Length=284, Percent_Identity=30.9859154929577, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24668781, Length=241, Percent_Identity=34.4398340248963, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356513, Length=231, Percent_Identity=35.0649350649351, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920494, Length=180, Percent_Identity=32.2222222222222, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: NA

Molecular weight: Translated: 42370; Mature: 42370

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLTTSSTARTASSSTRPRTGSTPRRRLWLWLWVELRSELSFPYYLRKRAKSLKFSAQT
CEEEECCCCCCCCCCCCCCCCCCCHHHEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHH
LFMLERLFSLGYSKTFAERYYQLWGERALAIAEAMEKPLPRCFRVNTLRVEIPTLTKLLN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCEEEEEHHHHHHHHH
KKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQEASSMYPPVALDPKPGEIVAD
HCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHEEEEEHHHCCCCCEEECCCCCHHHHH
MAAAPGGKTSYLAQLMKNEGIIYAFDVGEERLKETRLNLSRLGITNTILIHKSSLHMGEL
HHCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHCCCEEEEEEEECCCCCCCC
GIEFDKILLDAPCTGSGTIHKNPERKSNRTMEDIKFCQGLQMQMIKVALENLKPGGILVY
CCCEEHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
STCSLEPEENEFVIQWTLDSFDVELLPLRYGEPALIKPFGVKLSGEIKKARRFYPDRHGT
EEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCEECCHHHHHHHCCCCCCCC
SGFFVAKLRKL
CCEEHHHHHCC
>Mature Secondary Structure
MRLLTTSSTARTASSSTRPRTGSTPRRRLWLWLWVELRSELSFPYYLRKRAKSLKFSAQT
CEEEECCCCCCCCCCCCCCCCCCCHHHEEEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHH
LFMLERLFSLGYSKTFAERYYQLWGERALAIAEAMEKPLPRCFRVNTLRVEIPTLTKLLN
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEECCEEEEEHHHHHHHHH
KKGFQFRRVPWAREGFCLTREPFSITSTPEYLSGLLYIQEASSMYPPVALDPKPGEIVAD
HCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHEEEEEHHHCCCCCEEECCCCCHHHHH
MAAAPGGKTSYLAQLMKNEGIIYAFDVGEERLKETRLNLSRLGITNTILIHKSSLHMGEL
HHCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHCCCEEEEEEEECCCCCCCC
GIEFDKILLDAPCTGSGTIHKNPERKSNRTMEDIKFCQGLQMQMIKVALENLKPGGILVY
CCCEEHEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEE
STCSLEPEENEFVIQWTLDSFDVELLPLRYGEPALIKPFGVKLSGEIKKARRFYPDRHGT
EEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCEEECCCCEECCHHHHHHHCCCCCCCC
SGFFVAKLRKL
CCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]