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The map label for this gene is truA

Identifier: 212223540

GI number: 212223540

Start: 365169

End: 365972

Strand: Reverse

Name: truA

Synonym: TON_0394

Alternate gene names: 212223540

Gene position: 365972-365169 (Counterclockwise)

Preceding gene: 212223541

Following gene: 212223538

Centisome position: 19.81

GC content: 51.12

Gene sequence:

>804_bases
ATGAGGCTTGCCCTTCGTATAGCTTACGACGGAACTGCCTTCTATGGCTTTCAGAGGCAACCAGGCCTTAGAACCGTCGA
GGGGGAAATCATTAGGGCTCTCACTAAGCTGGGTATAATAGAGGACGTTGAGAGCTCCAACTTCAAGGGTGCATCAAGAA
CTGATCGGGGCGTTTCTGCATTCTTCAACGTTGTGGCCTTTGACGTTGCTTCGAGGCCGGATCTGGTCAGGCCTGAGGTG
CTCAACTTCCACCTGAAGGATGTCTGGGTTCTCGGAACTGCCGAGGTTCCGGAGGATTTCCACCCCCGCTTCTGGGCCAA
GTCAAAGACCTATCGCTATTACCTCATTGATGCGGGGTTTGATGAAAAGGCTATGAGGGAGTGCGCAAAGCTCTTCATCG
GAACTCACGACTTCTCAGCCTTTGCAAGGCTTGAGCCCCACAAAAACCCCGTGAGAGAACTGATTAGAGCCGATATCATC
AAACGCCAAGGATACTACATAATTGAGCTCGAAGGCAAAAGCTTCCTGTGGGAGATGGCCAGAAGAATAGTAAATGCACT
CCGCTTCTGTGGACTGGGCATCCTGACGGAGGAAGAAGTGGAGAAGATGCTCAACGGCGAGTACTCCAGGAAAATTCCAC
CGGCGAGACCGGAGGGGTTGGTGCTGTGGGGGATAGAATATGAAGGCATCGAGTTCAGAGGGAACGAGAAAGGAATCAGA
AAAGCAAAACACGACCTCTTCGAACGCTATTCAGAGGCATTAACCAGAGCTGCATTATTTGGCGACCTTCTTCTTGGTCT
TTAG

Upstream 100 bases:

>100_bases
TATCTGTCAAAATCTGCCCAATTTTTACATATATAATTGTCATAATGCCAACAGATTAAACACCAAAAAGGCTTTAACTT
TCACTTAACAACTTCCCATC

Downstream 100 bases:

>100_bases
GTTCATCTCCATGTCATAATAGCGGCCATAGTATTGCTTCAAAGCCTTCTGTCTCTCGAGGAAGTGCGTGAAGAGCAATG
GATCGTCGTCTTGGACGTCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRLALRIAYDGTAFYGFQRQPGLRTVEGEIIRALTKLGIIEDVESSNFKGASRTDRGVSAFFNVVAFDVASRPDLVRPEV
LNFHLKDVWVLGTAEVPEDFHPRFWAKSKTYRYYLIDAGFDEKAMRECAKLFIGTHDFSAFARLEPHKNPVRELIRADII
KRQGYYIIELEGKSFLWEMARRIVNALRFCGLGILTEEEVEKMLNGEYSRKIPPARPEGLVLWGIEYEGIEFRGNEKGIR
KAKHDLFERYSEALTRAALFGDLLLGL

Sequences:

>Translated_267_residues
MRLALRIAYDGTAFYGFQRQPGLRTVEGEIIRALTKLGIIEDVESSNFKGASRTDRGVSAFFNVVAFDVASRPDLVRPEV
LNFHLKDVWVLGTAEVPEDFHPRFWAKSKTYRYYLIDAGFDEKAMRECAKLFIGTHDFSAFARLEPHKNPVRELIRADII
KRQGYYIIELEGKSFLWEMARRIVNALRFCGLGILTEEEVEKMLNGEYSRKIPPARPEGLVLWGIEYEGIEFRGNEKGIR
KAKHDLFERYSEALTRAALFGDLLLGL
>Mature_267_residues
MRLALRIAYDGTAFYGFQRQPGLRTVEGEIIRALTKLGIIEDVESSNFKGASRTDRGVSAFFNVVAFDVASRPDLVRPEV
LNFHLKDVWVLGTAEVPEDFHPRFWAKSKTYRYYLIDAGFDEKAMRECAKLFIGTHDFSAFARLEPHKNPVRELIRADII
KRQGYYIIELEGKSFLWEMARRIVNALRFCGLGILTEEEVEKMLNGEYSRKIPPARPEGLVLWGIEYEGIEFRGNEKGIR
KAKHDLFERYSEALTRAALFGDLLLGL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=273, Percent_Identity=31.8681318681319, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI199559785, Length=310, Percent_Identity=25.1612903225806, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI70166645, Length=273, Percent_Identity=26.007326007326, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70166634, Length=273, Percent_Identity=26.007326007326, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70166599, Length=273, Percent_Identity=26.007326007326, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=27.2357723577236, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71985046, Length=312, Percent_Identity=26.2820512820513, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321112, Length=282, Percent_Identity=26.9503546099291, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362146, Length=271, Percent_Identity=26.5682656826568, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648455, Length=271, Percent_Identity=26.5682656826568, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_THEON (B6YTJ2)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306776.1
- GeneID:   7016689
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0394
- HOGENOM:   HBG621020
- OMA:   KSFLWEM
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30659; Mature: 30659

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: ACT_SITE 55-55

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLALRIAYDGTAFYGFQRQPGLRTVEGEIIRALTKLGIIEDVESSNFKGASRTDRGVSA
CEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHH
FFNVVAFDVASRPDLVRPEVLNFHLKDVWVLGTAEVPEDFHPRFWAKSKTYRYYLIDAGF
HHHHHHHHHCCCCCCCCHHHHEEEECEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
DEKAMRECAKLFIGTHDFSAFARLEPHKNPVRELIRADIIKRQGYYIIELEGKSFLWEMA
CHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
RRIVNALRFCGLGILTEEEVEKMLNGEYSRKIPPARPEGLVLWGIEYEGIEFRGNEKGIR
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEECCHHHHH
KAKHDLFERYSEALTRAALFGDLLLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLALRIAYDGTAFYGFQRQPGLRTVEGEIIRALTKLGIIEDVESSNFKGASRTDRGVSA
CEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHH
FFNVVAFDVASRPDLVRPEVLNFHLKDVWVLGTAEVPEDFHPRFWAKSKTYRYYLIDAGF
HHHHHHHHHCCCCCCCCHHHHEEEECEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCC
DEKAMRECAKLFIGTHDFSAFARLEPHKNPVRELIRADIIKRQGYYIIELEGKSFLWEMA
CHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
RRIVNALRFCGLGILTEEEVEKMLNGEYSRKIPPARPEGLVLWGIEYEGIEFRGNEKGIR
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCEEEECCHHHHH
KAKHDLFERYSEALTRAALFGDLLLGL
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA