Click here to switch to the map view.

The map label for this gene is pheT

Identifier: 212223541

GI number: 212223541

Start: 366326

End: 368044

Strand: Reverse

Name: pheT

Synonym: TON_0395

Alternate gene names: 212223541

Gene position: 368044-366326 (Counterclockwise)

Preceding gene: 212223542

Following gene: 212223540

Centisome position: 19.92

GC content: 52.82

Gene sequence:

>1719_bases
ATGCCGAAGTTCGATGTTTCGAAAGCGGATTTAGAGAGGCTCGTCGGAAAGAGCTTCACCGTTGAGGAATGGGAAGACCT
TTTCCTCTACGCGAAATGCGAGCTCGACGACGTTTGGGAGGAGAACGGTCAGATCTACTTCAAGGCAGACTCAAAGGACA
CCAACAGGCCAGACCTCTGGAGCGCCGAGGGAATAGCGAGGCAGATCCGCTGGGCACTTGGGATGGCGAAGGGCCTGCCA
AGGTATGAGGTCGAAAAGAGCGACGTCGTTGTTTACGTTGATGAAAAGCTGAAAGATATCAGGCCCTACGGTGTCTACGC
CATCGTTGAGGGCCTCAGCCTCGACGATGAGGCTCTCAAGCAGATGATCAACCTCCAGGAAAAGGTGGCGCTAACGTTCG
GAAGGCGCAGAAGGGAAGTTGCAATAGGCATCTTCGACTTCGACAAGGTTAAGCCGCCAATTTATTACCGCGCCGCTGAG
AAGACTGAGAAGTTCGTTCCGCTGGGCTTTGAGGAAAAGATGAGTCTCGAAGAAATCCTCGAGAAGCACGAGAAGGGCAA
GGAATACGGCCATCTGATAAAGGACAAGCCATATTACCCGCTCCTCGTCGACAGCGAGGGCAATGTCCTTTCCATGCCCC
CTATTATCAACTCCGAGCTGACCGGAAGGGTGACGACTGAGACCAGAAACGTCTTCGTCGACGTCACTGGCTGGGACTTG
AACAAGATTATGCTCGCCCTTAACGTTGTTGTCACTGCTCTGGCCGAGCGCGGCGGGAAGATAAAGAGTGTAAAGGTCGT
TTACGGAGACTTCGAGATAGAGACACCCAACCTAACTCCAAAGGAGTTCGAGGTTGAGTTCGAGTACATCAGGAGGCTCG
CAGGGATAGATCTGAGCGACGAAGAAATCAAGGAGCTCCTCGAGAGGATGTTTTACGAGGTGGAACTCGAAAATGGAAAG
GCCAAGCTCAAGTACCCGGCATTCCGCGATGACATAATGCACGCCCGTGATGTGCTCGAGGACGTCCTCATAGCTTACGG
CTACAACGAGATCAAACCGGAGGAGCCGAAGCTTGCCGTCCAGGGCAGGGGAGATAAGTTCGTTGAGTTTGAAGATACCG
TGAGGGAGCTCATGGTTGGCTACGGCCTTCAGGAGGTTATGACTTTCAACCTGACCAACAGGGAGGCTCAGTACACCAAG
ATGAACCTCGACTTCGGAGAGCATCCCTTTGAGGAGTACGGTCATCATCCGCCGGCGAGACTCGTGGAAATAGAGAATCC
CATAAGCCCCAAGTGGTCCGCTTTGAGGGCATGGCTGATTCCCAGCTTAATGGAGTTCCTGAGCCAGAACACCCACGAGG
AGTACCCGCAGAGAATATTCGAGGTAGGAAAGACAACGCTCATAAACGAGAACAAGGAGACCAAGACCGTAAGCGAGAGC
AAGCTTGCAGTTGCCATAGCCCATCCACGCGTAACTTTCACGGAAGTTAAAGAGATTCTCGACAGCGTTATGCACCACCT
CGGCCTCGAGTATGAGTTGAAAGAGATCGAGCACAACAGCTTCATCCCGGGAAGGGTCGGAAAAATCATCGTGAACGGTC
AGGAAGTAGGAATAATTGGGGAGATACACCCGAAGGTGCTGGAAAACTGGGGAATAGAAATGCCGGTGGCGGCCTTTGAG
GTCTTTCTGAGGCCGCTCTACAGGGAGCCGTATCTCTGA

Upstream 100 bases:

>100_bases
GCCATGTTCAAACTCGGAATCGACGACATAAGGTATCTCTTCAGCTACGACCTGGGGTGGCTGAGAGAGGCGAGGCTGGT
CTGGTAAAGGGGTGATGCTC

Downstream 100 bases:

>100_bases
CCTTTCTCATTATCTATCTTTTCGTTTTGAATAGAAAAGTGTGATGAAAATATCGGCTTGGCATCTTTTCCGACATTTTT
CGAGTCCCCACCAAAGTTCT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MPKFDVSKADLERLVGKSFTVEEWEDLFLYAKCELDDVWEENGQIYFKADSKDTNRPDLWSAEGIARQIRWALGMAKGLP
RYEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLSLDDEALKQMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAE
KTEKFVPLGFEEKMSLEEILEKHEKGKEYGHLIKDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETRNVFVDVTGWDL
NKIMLALNVVVTALAERGGKIKSVKVVYGDFEIETPNLTPKEFEVEFEYIRRLAGIDLSDEEIKELLERMFYEVELENGK
AKLKYPAFRDDIMHARDVLEDVLIAYGYNEIKPEEPKLAVQGRGDKFVEFEDTVRELMVGYGLQEVMTFNLTNREAQYTK
MNLDFGEHPFEEYGHHPPARLVEIENPISPKWSALRAWLIPSLMEFLSQNTHEEYPQRIFEVGKTTLINENKETKTVSES
KLAVAIAHPRVTFTEVKEILDSVMHHLGLEYELKEIEHNSFIPGRVGKIIVNGQEVGIIGEIHPKVLENWGIEMPVAAFE
VFLRPLYREPYL

Sequences:

>Translated_572_residues
MPKFDVSKADLERLVGKSFTVEEWEDLFLYAKCELDDVWEENGQIYFKADSKDTNRPDLWSAEGIARQIRWALGMAKGLP
RYEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLSLDDEALKQMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAE
KTEKFVPLGFEEKMSLEEILEKHEKGKEYGHLIKDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETRNVFVDVTGWDL
NKIMLALNVVVTALAERGGKIKSVKVVYGDFEIETPNLTPKEFEVEFEYIRRLAGIDLSDEEIKELLERMFYEVELENGK
AKLKYPAFRDDIMHARDVLEDVLIAYGYNEIKPEEPKLAVQGRGDKFVEFEDTVRELMVGYGLQEVMTFNLTNREAQYTK
MNLDFGEHPFEEYGHHPPARLVEIENPISPKWSALRAWLIPSLMEFLSQNTHEEYPQRIFEVGKTTLINENKETKTVSES
KLAVAIAHPRVTFTEVKEILDSVMHHLGLEYELKEIEHNSFIPGRVGKIIVNGQEVGIIGEIHPKVLENWGIEMPVAAFE
VFLRPLYREPYL
>Mature_571_residues
PKFDVSKADLERLVGKSFTVEEWEDLFLYAKCELDDVWEENGQIYFKADSKDTNRPDLWSAEGIARQIRWALGMAKGLPR
YEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLSLDDEALKQMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAEK
TEKFVPLGFEEKMSLEEILEKHEKGKEYGHLIKDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETRNVFVDVTGWDLN
KIMLALNVVVTALAERGGKIKSVKVVYGDFEIETPNLTPKEFEVEFEYIRRLAGIDLSDEEIKELLERMFYEVELENGKA
KLKYPAFRDDIMHARDVLEDVLIAYGYNEIKPEEPKLAVQGRGDKFVEFEDTVRELMVGYGLQEVMTFNLTNREAQYTKM
NLDFGEHPFEEYGHHPPARLVEIENPISPKWSALRAWLIPSLMEFLSQNTHEEYPQRIFEVGKTTLINENKETKTVSESK
LAVAIAHPRVTFTEVKEILDSVMHHLGLEYELKEIEHNSFIPGRVGKIIVNGQEVGIIGEIHPKVLENWGIEMPVAAFEV
FLRPLYREPYL

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=605, Percent_Identity=27.7685950413223, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI24308207, Length=165, Percent_Identity=33.9393939393939, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788006, Length=429, Percent_Identity=24.009324009324, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17533403, Length=611, Percent_Identity=29.7872340425532, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6323089, Length=604, Percent_Identity=25.9933774834437, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21355211, Length=602, Percent_Identity=28.5714285714286, Blast_Score=251, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_THEON (B6YTJ3)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306777.1
- GeneID:   7016690
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0395
- HOGENOM:   HBG497667
- OMA:   LLTRMCL
- ProtClustDB:   PRK09616
- GO:   GO:0005737
- HAMAP:   MF_00284
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918
- Gene3D:   G3DSA:3.30.56.20
- SMART:   SM00873
- SMART:   SM00874
- TIGRFAMs:   TIGR00471

Pfam domain/function: PF03483 B3_4; PF03484 B5; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 66042; Mature: 65911

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS51483 B5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFDVSKADLERLVGKSFTVEEWEDLFLYAKCELDDVWEENGQIYFKADSKDTNRPDLW
CCCCCCCHHHHHHHHCCCCCHHHHCCEEEEEEECHHHHHCCCCEEEEEECCCCCCCCCCC
SAEGIARQIRWALGMAKGLPRYEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLSLDDEALK
CHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEECCHHHCCCCCCEEEEEECCCCCHHHHH
QMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAEKTEKFVPLGFEEKMSLEEIL
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCHHCCCHHHHH
EKHEKGKEYGHLIKDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETRNVFVDVTGWDL
HHHHCCHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEECCEEEEEECCCCH
NKIMLALNVVVTALAERGGKIKSVKVVYGDFEIETPNLTPKEFEVEFEYIRRLAGIDLSD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHEEEHHHHHHHHCCCCCH
EEIKELLERMFYEVELENGKAKLKYPAFRDDIMHARDVLEDVLIAYGYNEIKPEEPKLAV
HHHHHHHHHHHEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
QGRGDKFVEFEDTVRELMVGYGLQEVMTFNLTNREAQYTKMNLDFGEHPFEEYGHHPPAR
ECCCCCEEEHHHHHHHHHHHCCHHHHHEECCCCCCCEEEEEECCCCCCCHHHHCCCCCCE
LVEIENPISPKWSALRAWLIPSLMEFLSQNTHEEYPQRIFEVGKTTLINENKETKTVSES
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCC
KLAVAIAHPRVTFTEVKEILDSVMHHLGLEYELKEIEHNSFIPGRVGKIIVNGQEVGIIG
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCCEEEECCCEEEEEE
EIHPKVLENWGIEMPVAAFEVFLRPLYREPYL
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKFDVSKADLERLVGKSFTVEEWEDLFLYAKCELDDVWEENGQIYFKADSKDTNRPDLW
CCCCCCHHHHHHHHCCCCCHHHHCCEEEEEEECHHHHHCCCCEEEEEECCCCCCCCCCC
SAEGIARQIRWALGMAKGLPRYEVEKSDVVVYVDEKLKDIRPYGVYAIVEGLSLDDEALK
CHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEECCHHHCCCCCCEEEEEECCCCCHHHHH
QMINLQEKVALTFGRRRREVAIGIFDFDKVKPPIYYRAAEKTEKFVPLGFEEKMSLEEIL
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCHHCCCHHHHH
EKHEKGKEYGHLIKDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETRNVFVDVTGWDL
HHHHCCHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEEECCEEEEEECCCCH
NKIMLALNVVVTALAERGGKIKSVKVVYGDFEIETPNLTPKEFEVEFEYIRRLAGIDLSD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCHHHEEEHHHHHHHHCCCCCH
EEIKELLERMFYEVELENGKAKLKYPAFRDDIMHARDVLEDVLIAYGYNEIKPEEPKLAV
HHHHHHHHHHHEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
QGRGDKFVEFEDTVRELMVGYGLQEVMTFNLTNREAQYTKMNLDFGEHPFEEYGHHPPAR
ECCCCCEEEHHHHHHHHHHHCCHHHHHEECCCCCCCEEEEEECCCCCCCHHHHCCCCCCE
LVEIENPISPKWSALRAWLIPSLMEFLSQNTHEEYPQRIFEVGKTTLINENKETKTVSES
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCC
KLAVAIAHPRVTFTEVKEILDSVMHHLGLEYELKEIEHNSFIPGRVGKIIVNGQEVGIIG
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCCEEEECCCEEEEEE
EIHPKVLENWGIEMPVAAFEVFLRPLYREPYL
CCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA