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The map label for this gene is hyfD [H]

Identifier: 212223425

GI number: 212223425

Start: 246353

End: 247804

Strand: Reverse

Name: hyfD [H]

Synonym: TON_0279

Alternate gene names: 212223425

Gene position: 247804-246353 (Counterclockwise)

Preceding gene: 212223426

Following gene: 212223424

Centisome position: 13.41

GC content: 53.51

Gene sequence:

>1452_bases
ATGAACGCCTCTCCCTTCATTATATCTTTTTTGATCCCCCTGCTCCTCGGTCCACTCCTGTTCAAATTAGACGGTAGAAA
GGCCGATGTATTCATGCTCATCGCCGTTGTGTCTTCCTTCCTGGCTAATCTTGTGGGAGTCCTCGAATACCTGAAAGTCG
GTGGCGCTCATCATATCGTTTACCTCGAAACTTCTTCCCTCGGTGAGGTCTACGGCGTTATAATCGACCCAATGAGCGTT
TTGGTCGGTTTTGTCGTGAGCTTGGCTGGCGTGCTGTTCCTTCTCTACGCGGTGGACTACATGAGCGAGAGAAACAAGCA
GCACCCCGTCTACTCTGATAAGGGCAGGTTCTATGCTTGGATGGTCATCTTCGTTGGAGCTACGCTGGCATTCATATACT
CCTCCACGACGCTTCAGCTGCTCATATTCTTCGAGATAATGGGACTCGCCTGCTGGGGTGTCGTTGGGTATTATAAGGGC
CCAAAAGCCGAGAGGGCTGCATACAAGGCCCTGCTTGTGCCGAACTTCGGTGCCATGGTGGGCCTCTACACCACCGTTGG
CATTGGCATCCTCAAGCTCCATGATTTGAGCATCTATGCGCTCCAGAACCTGAATGATGAGCTCAAGCTTCTCGTGTTCC
TTGGCGTAATGGTTGCGGCCTTTACCAAGAGCGCCCAGTTCCCGCTCTATTCATGGCTTCCGGATGCAATGGCGGCGCCG
ACACCTGCTTCCGCTTTTCTCCACGGTGCTGCAATGGTTGAAATGGGCGTTTACCTGCTCGCCAGGGTCACCCAGTTCAT
GCAACCGATTCCGGAGACAGCTTTCTACGTTATGCTCGTCTTCGTGTCGCTAACTTTACTCATAGCAATTCTCTACTACC
CGCTCCAGAAGGACGCCAAGAGACTCCTTGCTTATTCAACCATAGCAGAGGCAGGAGTGATGTACGTTGGCGTGCTCTAT
GCCGTGCTTGGCTCTGTCTATGGTCTCCAGGCGGCCATGTTCCAGCTGGCTAACCACGCTTTCGTCAAGGGTCTTGCCTT
CCTCACCGCGGGAACCTTCAGTTACGCTTTTGGAACGCTCGACATGGAGAAGATTAGGGGCCTCGGAAAGCTCGTTCCGG
TCGTTGGTGCAAGCTGGTTCTTAGCCCTTCTCGGCCTGGCTGGAGTTCCTCCGCTCGGCCTGTTCTTCAGCAAGGCGTAT
CTCTTCATGAACGCGTCTTCAATAACCAGCTGGGTTGGCTGGATTCCGCTCTTCCTAGTGTTGGCCGATGCCACGGTTTT
CCTTGCGGTATCTCTCGGATGGATTAAGAGGATGGTATTCAGCGAGCCCCTCCAGGAGAGTGCAGAAGTTTCCCCGCTGA
TGCGCTTTGTCCTCGTAGTCCTAATAGTCCTGTCCATCGTTGCGCCGTTCCTAAGCGTGAAGCTCGTGACTCAGATAGGG
TTCATGGGGTGA

Upstream 100 bases:

>100_bases
TCTGGGCCAGAGAAGGAAAGATAGTCGCCGAGCTTAAGGACATCGGCGACAGGACCAACGTCCTCGAGGCCTACCTCATC
AGATGAAAGGAGGTGCCAGG

Downstream 100 bases:

>100_bases
TGTGGGATGATGGAAATTCCAATCGCGCTCTACTCACTCTCAGCGATTTCCGGCCTGATTGGAGACTTTAAGCGGAGCAT
TAAGATTTCAAGCGTCCTCT

Product: hydrogenase 4 subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNASPFIISFLIPLLLGPLLFKLDGRKADVFMLIAVVSSFLANLVGVLEYLKVGGAHHIVYLETSSLGEVYGVIIDPMSV
LVGFVVSLAGVLFLLYAVDYMSERNKQHPVYSDKGRFYAWMVIFVGATLAFIYSSTTLQLLIFFEIMGLACWGVVGYYKG
PKAERAAYKALLVPNFGAMVGLYTTVGIGILKLHDLSIYALQNLNDELKLLVFLGVMVAAFTKSAQFPLYSWLPDAMAAP
TPASAFLHGAAMVEMGVYLLARVTQFMQPIPETAFYVMLVFVSLTLLIAILYYPLQKDAKRLLAYSTIAEAGVMYVGVLY
AVLGSVYGLQAAMFQLANHAFVKGLAFLTAGTFSYAFGTLDMEKIRGLGKLVPVVGASWFLALLGLAGVPPLGLFFSKAY
LFMNASSITSWVGWIPLFLVLADATVFLAVSLGWIKRMVFSEPLQESAEVSPLMRFVLVVLIVLSIVAPFLSVKLVTQIG
FMG

Sequences:

>Translated_483_residues
MNASPFIISFLIPLLLGPLLFKLDGRKADVFMLIAVVSSFLANLVGVLEYLKVGGAHHIVYLETSSLGEVYGVIIDPMSV
LVGFVVSLAGVLFLLYAVDYMSERNKQHPVYSDKGRFYAWMVIFVGATLAFIYSSTTLQLLIFFEIMGLACWGVVGYYKG
PKAERAAYKALLVPNFGAMVGLYTTVGIGILKLHDLSIYALQNLNDELKLLVFLGVMVAAFTKSAQFPLYSWLPDAMAAP
TPASAFLHGAAMVEMGVYLLARVTQFMQPIPETAFYVMLVFVSLTLLIAILYYPLQKDAKRLLAYSTIAEAGVMYVGVLY
AVLGSVYGLQAAMFQLANHAFVKGLAFLTAGTFSYAFGTLDMEKIRGLGKLVPVVGASWFLALLGLAGVPPLGLFFSKAY
LFMNASSITSWVGWIPLFLVLADATVFLAVSLGWIKRMVFSEPLQESAEVSPLMRFVLVVLIVLSIVAPFLSVKLVTQIG
FMG
>Mature_483_residues
MNASPFIISFLIPLLLGPLLFKLDGRKADVFMLIAVVSSFLANLVGVLEYLKVGGAHHIVYLETSSLGEVYGVIIDPMSV
LVGFVVSLAGVLFLLYAVDYMSERNKQHPVYSDKGRFYAWMVIFVGATLAFIYSSTTLQLLIFFEIMGLACWGVVGYYKG
PKAERAAYKALLVPNFGAMVGLYTTVGIGILKLHDLSIYALQNLNDELKLLVFLGVMVAAFTKSAQFPLYSWLPDAMAAP
TPASAFLHGAAMVEMGVYLLARVTQFMQPIPETAFYVMLVFVSLTLLIAILYYPLQKDAKRLLAYSTIAEAGVMYVGVLY
AVLGSVYGLQAAMFQLANHAFVKGLAFLTAGTFSYAFGTLDMEKIRGLGKLVPVVGASWFLALLGLAGVPPLGLFFSKAY
LFMNASSITSWVGWIPLFLVLADATVFLAVSLGWIKRMVFSEPLQESAEVSPLMRFVLVVLIVLSIVAPFLSVKLVTQIG
FMG

Specific function: Unknown

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=297, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1788829, Length=416, Percent_Identity=38.4615384615385, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788614, Length=339, Percent_Identity=33.3333333333333, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=30.1675977653631, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788831, Length=341, Percent_Identity=26.9794721407625, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI2367154, Length=342, Percent_Identity=23.9766081871345, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52651; Mature: 52651

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCC
>Mature Secondary Structure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CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]