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The map label for this gene is hyfB [H]

Identifier: 212223424

GI number: 212223424

Start: 244367

End: 246346

Strand: Reverse

Name: hyfB [H]

Synonym: TON_0278

Alternate gene names: 212223424

Gene position: 246346-244367 (Counterclockwise)

Preceding gene: 212223425

Following gene: 212223423

Centisome position: 13.33

GC content: 54.85

Gene sequence:

>1980_bases
ATGATGGAAATTCCAATCGCGCTCTACTCACTCTCAGCGATTTCCGGCCTGATTGGAGACTTTAAGCGGAGCATTAAGAT
TTCAAGCGTCCTCTCAGCCATAGCATCCCTATCCCTTCTGGGCATAGCTGCCCACGCCCTGTCCAGGGGGCTTCCCGTTC
AGGAGAGCTTTTTGGGCATTCCCCTAATCATAGACAGCCTCTCCCTCCCGTTCCTGTTCATCATAGCCCTGCTCAGCCTT
GTGGTTTCAGTGTATTCCATTTCATATATGGAAGTCCACAGAGATACCGGAAGACCACTGGCGTACACCATCCTCTACGG
CACGTTCGTGCTGTCGATTGTATTCGTGGCTCTGACGTCAAACCTGCTCTGGTTCGTCTTCTTCTGGGAGCTGATGACCC
TAACTTCCTTCATCTTCGTGAGCTGGAGGGAGCAGGACGCTGGAATTAAATACCTCCTCACGATGCAGCTCGCCAACACG
GTGCCCCTCTTCGTGGCCCTCGGCATAATCTACTCCGCCACTGGAAGCTTCAGCGTTGATTACGCCACGCTTAGGGAGGT
TGCATCTTCCCTTTCTCCAGTCCAGCTCAAGCTGCTCTACGCGATGTTCCTCGTGACGTTCCTTGCAAAATCTGGAAGCG
TGCCCTTCCAGTTCTGGGTGCCCGATGCATACGAAGCCGCTCCCAGCAATATAGCCTCGCTGATGGCCGGCGTCATGGAG
AAGATGGCGGTTTACGGTCTGATAAGGCTCCTCTGCAACGCCCTGCCATGCAGTGAAGGCATTGGTTACGTTCTCGTTAT
CGTCGGCATACTTACCATGACCTTCGGAACCCTCTACGCCCTCAGAGAGACTCACGCAAAGAGGCTCCTCGCTTACTCAA
GCGTTGGACAAATGGGCTACATCTGGTTCGCGGTGGGCATGGGCATGATCTTCCTGACGATGGGCATGGAGAGCCTGGCT
TACCTGGCCTTCCTCGCCGGAGTCTTCCACTCCTTCAATCACACACTCTTCAAGGGGCTGCTCTTTCTCATCTCGGGCAA
CTTCGAGTACTCCGCCGGAACCGCTGACCTCAACGAGCTTGGTGGTTTGAGGAGGGCAATGCCGTACTCGTCGCTCTTCA
CCGTCATAGGTGCGCTCTCCCTCGCTGGAGTGCCCCTCTTCAGCGGTTTCCTCTCCAAGTGGATGATTTACCAGGCCGGC
TACTACTCTGGAATCGGCCTCTTCGTCTTTGGCTCCGTAATGGCGGTGTTTATGAGCGCCGTAACCTTGGCATATTCGCT
CAAGCTCTACACCTCTGCCTTTGGGGGCGAACCGAACGAGAGAACTGAGAACGCCAGGGAAGTCCCGTCGGGTATGCTCC
TCGGTGAGGGAATTATTGCCTTAACTTCACTTGCCGTTGGAATACTTCCGGCTATTGCTTACCCGATATTAACGATTTCA
TTGAATGGCGGCGACGTCACCGTTACAATGGGCTCGATATCCACTGACTTTGAGTACTTCTCGCCAATAGCCCTGCTCCT
TGCGGTTTCATTCATTGCGGTTGCTTCATACTTCGTCTTCAGGCCAAAGACGACCAATGTCAAACCCTGGAACACTGGAG
CGCTTTTCCTGCCGGAGGAGAGGTATGGAGCGAAGGCCAGGGACTATTACAGGCAGTACTTTACCGAGATGGAGGGCCTC
TACAAGCTTGGAAGCGCCGCTGGCAAGGTCGGAAGGGTCCTTCTCTCTGCTCTGATGTCCGTCTACCTCGTTCTCGCCAG
GGGCCTCGTCTACACCGGCAGGGAGAAGAAGCGCTCCTTCACCCTTGACGAGCTTCGCCACCGCACCGTCAGGTACCTGG
ACGAGGCATTCTTCGCGCCGATGATGGATCTACTCAAAAACATCGCCGTGCTGGCAGCGGGCATCTCGGTGTCCATGGAC
GAGCTCTTCCTGGCTTCAATGCTGACCACGGTGATAATACTCGCACTCCTTGTGTTGTGA

Upstream 100 bases:

>100_bases
GATGCGCTTTGTCCTCGTAGTCCTAATAGTCCTGTCCATCGTTGCGCCGTTCCTAAGCGTGAAGCTCGTGACTCAGATAG
GGTTCATGGGGTGATGTGGG

Downstream 100 bases:

>100_bases
GGTGATGGTGATGGACTACGTAAGCATTATCGCTGCTCCGATCGTCCTCTTCCTCCTTCCACCGTTCCTTGACGGAATAG
GGAGAAGGATAAAGGCGAGG

Product: Hydrogenase 4, component B or formate hydrogen lyase, subunit 3

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MMEIPIALYSLSAISGLIGDFKRSIKISSVLSAIASLSLLGIAAHALSRGLPVQESFLGIPLIIDSLSLPFLFIIALLSL
VVSVYSISYMEVHRDTGRPLAYTILYGTFVLSIVFVALTSNLLWFVFFWELMTLTSFIFVSWREQDAGIKYLLTMQLANT
VPLFVALGIIYSATGSFSVDYATLREVASSLSPVQLKLLYAMFLVTFLAKSGSVPFQFWVPDAYEAAPSNIASLMAGVME
KMAVYGLIRLLCNALPCSEGIGYVLVIVGILTMTFGTLYALRETHAKRLLAYSSVGQMGYIWFAVGMGMIFLTMGMESLA
YLAFLAGVFHSFNHTLFKGLLFLISGNFEYSAGTADLNELGGLRRAMPYSSLFTVIGALSLAGVPLFSGFLSKWMIYQAG
YYSGIGLFVFGSVMAVFMSAVTLAYSLKLYTSAFGGEPNERTENAREVPSGMLLGEGIIALTSLAVGILPAIAYPILTIS
LNGGDVTVTMGSISTDFEYFSPIALLLAVSFIAVASYFVFRPKTTNVKPWNTGALFLPEERYGAKARDYYRQYFTEMEGL
YKLGSAAGKVGRVLLSALMSVYLVLARGLVYTGREKKRSFTLDELRHRTVRYLDEAFFAPMMDLLKNIAVLAAGISVSMD
ELFLASMLTTVIILALLVL

Sequences:

>Translated_659_residues
MMEIPIALYSLSAISGLIGDFKRSIKISSVLSAIASLSLLGIAAHALSRGLPVQESFLGIPLIIDSLSLPFLFIIALLSL
VVSVYSISYMEVHRDTGRPLAYTILYGTFVLSIVFVALTSNLLWFVFFWELMTLTSFIFVSWREQDAGIKYLLTMQLANT
VPLFVALGIIYSATGSFSVDYATLREVASSLSPVQLKLLYAMFLVTFLAKSGSVPFQFWVPDAYEAAPSNIASLMAGVME
KMAVYGLIRLLCNALPCSEGIGYVLVIVGILTMTFGTLYALRETHAKRLLAYSSVGQMGYIWFAVGMGMIFLTMGMESLA
YLAFLAGVFHSFNHTLFKGLLFLISGNFEYSAGTADLNELGGLRRAMPYSSLFTVIGALSLAGVPLFSGFLSKWMIYQAG
YYSGIGLFVFGSVMAVFMSAVTLAYSLKLYTSAFGGEPNERTENAREVPSGMLLGEGIIALTSLAVGILPAIAYPILTIS
LNGGDVTVTMGSISTDFEYFSPIALLLAVSFIAVASYFVFRPKTTNVKPWNTGALFLPEERYGAKARDYYRQYFTEMEGL
YKLGSAAGKVGRVLLSALMSVYLVLARGLVYTGREKKRSFTLDELRHRTVRYLDEAFFAPMMDLLKNIAVLAAGISVSMD
ELFLASMLTTVIILALLVL
>Mature_659_residues
MMEIPIALYSLSAISGLIGDFKRSIKISSVLSAIASLSLLGIAAHALSRGLPVQESFLGIPLIIDSLSLPFLFIIALLSL
VVSVYSISYMEVHRDTGRPLAYTILYGTFVLSIVFVALTSNLLWFVFFWELMTLTSFIFVSWREQDAGIKYLLTMQLANT
VPLFVALGIIYSATGSFSVDYATLREVASSLSPVQLKLLYAMFLVTFLAKSGSVPFQFWVPDAYEAAPSNIASLMAGVME
KMAVYGLIRLLCNALPCSEGIGYVLVIVGILTMTFGTLYALRETHAKRLLAYSSVGQMGYIWFAVGMGMIFLTMGMESLA
YLAFLAGVFHSFNHTLFKGLLFLISGNFEYSAGTADLNELGGLRRAMPYSSLFTVIGALSLAGVPLFSGFLSKWMIYQAG
YYSGIGLFVFGSVMAVFMSAVTLAYSLKLYTSAFGGEPNERTENAREVPSGMLLGEGIIALTSLAVGILPAIAYPILTIS
LNGGDVTVTMGSISTDFEYFSPIALLLAVSFIAVASYFVFRPKTTNVKPWNTGALFLPEERYGAKARDYYRQYFTEMEGL
YKLGSAAGKVGRVLLSALMSVYLVLARGLVYTGREKKRSFTLDELRHRTVRYLDEAFFAPMMDLLKNIAVLAAGISVSMD
ELFLASMLTTVIILALLVL

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=395, Percent_Identity=24.5569620253165, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI251831116, Length=406, Percent_Identity=24.6305418719212, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788827, Length=421, Percent_Identity=33.4916864608076, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788829, Length=406, Percent_Identity=28.3251231527094, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788831, Length=360, Percent_Identity=31.3888888888889, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI2367154, Length=414, Percent_Identity=27.536231884058, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1788614, Length=414, Percent_Identity=29.2270531400966, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=26.3888888888889, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693160, Length=461, Percent_Identity=27.5488069414317, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 71949; Mature: 71949

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEIPIALYSLSAISGLIGDFKRSIKISSVLSAIASLSLLGIAAHALSRGLPVQESFLGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
PLIIDSLSLPFLFIIALLSLVVSVYSISYMEVHRDTGRPLAYTILYGTFVLSIVFVALTS
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLWFVFFWELMTLTSFIFVSWREQDAGIKYLLTMQLANTVPLFVALGIIYSATGSFSVD
HHHHHHHHHHHHHHHHHHHHEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEH
YATLREVASSLSPVQLKLLYAMFLVTFLAKSGSVPFQFWVPDAYEAAPSNIASLMAGVME
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCHHHHHHHHHHHHH
KMAVYGLIRLLCNALPCSEGIGYVLVIVGILTMTFGTLYALRETHAKRLLAYSSVGQMGY
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IWFAVGMGMIFLTMGMESLAYLAFLAGVFHSFNHTLFKGLLFLISGNFEYSAGTADLNEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHH
GGLRRAMPYSSLFTVIGALSLAGVPLFSGFLSKWMIYQAGYYSGIGLFVFGSVMAVFMSA
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHH
VTLAYSLKLYTSAFGGEPNERTENAREVPSGMLLGEGIIALTSLAVGILPAIAYPILTIS
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
LNGGDVTVTMGSISTDFEYFSPIALLLAVSFIAVASYFVFRPKTTNVKPWNTGALFLPEE
ECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHH
RYGAKARDYYRQYFTEMEGLYKLGSAAGKVGRVLLSALMSVYLVLARGLVYTGREKKRSF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCHHHHCC
TLDELRHRTVRYLDEAFFAPMMDLLKNIAVLAAGISVSMDELFLASMLTTVIILALLVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MMEIPIALYSLSAISGLIGDFKRSIKISSVLSAIASLSLLGIAAHALSRGLPVQESFLGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
PLIIDSLSLPFLFIIALLSLVVSVYSISYMEVHRDTGRPLAYTILYGTFVLSIVFVALTS
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
NLLWFVFFWELMTLTSFIFVSWREQDAGIKYLLTMQLANTVPLFVALGIIYSATGSFSVD
HHHHHHHHHHHHHHHHHHHHEECCCCCCHHEEHHHHHHHHHHHHHHHHHHHHCCCCCEEH
YATLREVASSLSPVQLKLLYAMFLVTFLAKSGSVPFQFWVPDAYEAAPSNIASLMAGVME
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHCCHHHHHHHHHHHHH
KMAVYGLIRLLCNALPCSEGIGYVLVIVGILTMTFGTLYALRETHAKRLLAYSSVGQMGY
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IWFAVGMGMIFLTMGMESLAYLAFLAGVFHSFNHTLFKGLLFLISGNFEYSAGTADLNEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHHHH
GGLRRAMPYSSLFTVIGALSLAGVPLFSGFLSKWMIYQAGYYSGIGLFVFGSVMAVFMSA
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHH
VTLAYSLKLYTSAFGGEPNERTENAREVPSGMLLGEGIIALTSLAVGILPAIAYPILTIS
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
LNGGDVTVTMGSISTDFEYFSPIALLLAVSFIAVASYFVFRPKTTNVKPWNTGALFLPEE
ECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHH
RYGAKARDYYRQYFTEMEGLYKLGSAAGKVGRVLLSALMSVYLVLARGLVYTGREKKRSF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCHHHHCC
TLDELRHRTVRYLDEAFFAPMMDLLKNIAVLAAGISVSMDELFLASMLTTVIILALLVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]