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The map label for this gene is hycG [H]

Identifier: 212223420

GI number: 212223420

Start: 240285

End: 241085

Strand: Reverse

Name: hycG [H]

Synonym: TON_0274

Alternate gene names: 212223420

Gene position: 241085-240285 (Counterclockwise)

Preceding gene: 212223421

Following gene: 212223419

Centisome position: 13.05

GC content: 55.43

Gene sequence:

>801_bases
ATGAGCGGGTTGAAGTCCGTTTGGGTCTTCCACGTTGACAGTGGGAGCTGCAACGGCTGCGACATAGAGATACTCGACGT
GCTCACGCCCTATTACGACGCCGAGAGGCTTGGGATAAAGCTCGTGCCGAGTCCAAGACATGCCGATGCCCTCCTCGTTT
CAGGCCCACTCACGAGGCAGACTTACTACGCTGTCAAAGCAGCCTACGAGGCGATGCCGCCGAAGCCGAGGATAGTTGTG
GCCATAGGCACCTGCGCGTCCAGTGGTGGTATATTCTACAACGGTTACCCAATCTACAACCCGAACCCTGAGAGGGGAAG
CGACAGGCTCAGGACGGGTGGAATAGAGGTCCTTTTGGCGGAGTACGGGAAAAAGCCCGACATGTACATTCCAGGATGTC
CACCGAGTCCGGAGGAGATACTATATGGGCTGGCCCAGCTCCTCGGCCTGAAGGAGAAGAAGATGAAGGGCGAGTACTAC
TATGCAGACGAGATTGAGTTCGTTCTTCCAGAGAGACCCATCGAGGAGAGGATTTACCTGACGCTCAGAGAATCCCTGAG
GCGCGTCGTGGGGTACTTCGACAGGGAGAAGGTTCTCGAGGACTTCATGGCCCTCGTGGAAAAGGCTCAGGAGAGCGAGA
ACCCGAGGGAGAGGCTCCACGAGCTAGTCATCGGATACTTCCTGAGGGAGAAGGATTCCCGTGTGAAGTTCGCGATAAGG
TTCCTCGAAAACGAGTACTGGAGGTTGAAGGATGCCTACGAAAAGAGGCACCTGGCACTTGTTAAAGCTGGTGTACGTTA
A

Upstream 100 bases:

>100_bases
TGGAGGAGAGCGCCAAGACCTTGGCGGTTCAAGGGCCCTATGCGGATAGCCTTCTCCTTTCCCTCTATCCGAGAGGCTCA
AAGGTGATGGGTGAGAGGAG

Downstream 100 bases:

>100_bases
TCCACCTTACGTCTCGAAGGCGATGGCACATTTCGCCGGCGACATGGCTGTGTTAGAGGAAATAGGCACGCTGAATGAGA
AAAGCGTCGAGATAATCAAG

Product: Hydrogenase 4, component I or formate hydrogen lyase, subunit 7

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSGLKSVWVFHVDSGSCNGCDIEILDVLTPYYDAERLGIKLVPSPRHADALLVSGPLTRQTYYAVKAAYEAMPPKPRIVV
AIGTCASSGGIFYNGYPIYNPNPERGSDRLRTGGIEVLLAEYGKKPDMYIPGCPPSPEEILYGLAQLLGLKEKKMKGEYY
YADEIEFVLPERPIEERIYLTLRESLRRVVGYFDREKVLEDFMALVEKAQESENPRERLHELVIGYFLREKDSRVKFAIR
FLENEYWRLKDAYEKRHLALVKAGVR

Sequences:

>Translated_266_residues
MSGLKSVWVFHVDSGSCNGCDIEILDVLTPYYDAERLGIKLVPSPRHADALLVSGPLTRQTYYAVKAAYEAMPPKPRIVV
AIGTCASSGGIFYNGYPIYNPNPERGSDRLRTGGIEVLLAEYGKKPDMYIPGCPPSPEEILYGLAQLLGLKEKKMKGEYY
YADEIEFVLPERPIEERIYLTLRESLRRVVGYFDREKVLEDFMALVEKAQESENPRERLHELVIGYFLREKDSRVKFAIR
FLENEYWRLKDAYEKRHLALVKAGVR
>Mature_265_residues
SGLKSVWVFHVDSGSCNGCDIEILDVLTPYYDAERLGIKLVPSPRHADALLVSGPLTRQTYYAVKAAYEAMPPKPRIVVA
IGTCASSGGIFYNGYPIYNPNPERGSDRLRTGGIEVLLAEYGKKPDMYIPGCPPSPEEILYGLAQLLGLKEKKMKGEYYY
ADEIEFVLPERPIEERIYLTLRESLRRVVGYFDREKVLEDFMALVEKAQESENPRERLHELVIGYFLREKDSRVKFAIRF
LENEYWRLKDAYEKRHLALVKAGVR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=139, Percent_Identity=36.6906474820144, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1789074, Length=230, Percent_Identity=36.0869565217391, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788834, Length=202, Percent_Identity=36.1386138613861, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=31.25, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=33.0985915492958, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI18859983, Length=137, Percent_Identity=37.2262773722628, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24642371, Length=137, Percent_Identity=37.2262773722628, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=36.4963503649635, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 30450; Mature: 30319

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLKSVWVFHVDSGSCNGCDIEILDVLTPYYDAERLGIKLVPSPRHADALLVSGPLTRQ
CCCCCEEEEEEECCCCCCCCCEEHHHHHCCCCCHHHCCEEECCCCCCCCEEEECCCCCHH
TYYAVKAAYEAMPPKPRIVVAIGTCASSGGIFYNGYPIYNPNPERGSDRLRTGGIEVLLA
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCEECCCCCCCCCCCHHHHCCCEEEEE
EYGKKPDMYIPGCPPSPEEILYGLAQLLGLKEKKMKGEYYYADEIEFVLPERPIEERIYL
HHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCEEEECCCCCHHHHHHH
TLRESLRRVVGYFDREKVLEDFMALVEKAQESENPRERLHELVIGYFLREKDSRVKFAIR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
FLENEYWRLKDAYEKRHLALVKAGVR
HHHCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLKSVWVFHVDSGSCNGCDIEILDVLTPYYDAERLGIKLVPSPRHADALLVSGPLTRQ
CCCCEEEEEEECCCCCCCCCEEHHHHHCCCCCHHHCCEEECCCCCCCCEEEECCCCCHH
TYYAVKAAYEAMPPKPRIVVAIGTCASSGGIFYNGYPIYNPNPERGSDRLRTGGIEVLLA
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEECCEECCCCCCCCCCCHHHHCCCEEEEE
EYGKKPDMYIPGCPPSPEEILYGLAQLLGLKEKKMKGEYYYADEIEFVLPERPIEERIYL
HHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCEEEECCCCCHHHHHHH
TLRESLRRVVGYFDREKVLEDFMALVEKAQESENPRERLHELVIGYFLREKDSRVKFAIR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
FLENEYWRLKDAYEKRHLALVKAGVR
HHHCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]