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The map label for this gene is gatA [H]

Identifier: 209554383

GI number: 209554383

Start: 762620

End: 764053

Strand: Reverse

Name: gatA [H]

Synonym: UUR10_0636

Alternate gene names: 209554383

Gene position: 764053-762620 (Counterclockwise)

Preceding gene: 209554271

Following gene: 209554532

Centisome position: 87.37

GC content: 26.22

Gene sequence:

>1434_bases
ATGAAAAATAAAACTATTTTAACACTTCATGAACAAATTAAAAAACGTGAAATTAAAATTTATGATTTAGTTAAAAAAAC
AATTGATCATAATTTAAAAATAAAAAATTTAAATGCAAGTTTAGCAAATAATTATGAACAAGCATTAAATCAAGCATCTT
TTTTAGACCAAGAAATTTCAAATAATGACACGCAAATTGATTATTTATTTGGAATTCCATATTCTTTAAAAGACAATATT
TCAACTAAAAATATCATTACAACAGGTGGTTCATTATTTTTAGAAAATTATCAACCACCATATGATGCAACAGTTAAAAC
ACTATTAGATCAAAACCAAGCGATTTTATTAAATAAATCAAATTTAGATGAATTTGGTTTAGGTGGTACTGGAACTGATT
CTGCTTTTGGTATAGTTGTTAATCCATTAGATTCAACACGTGTGTGTGGTGGTTCATCTTCTGGTAGTGCAGTATTAGTA
CAACAAGGTGTTGTGCCTTTTGCAATTGCTACAGACACAGGTGATTCTATTCGTCGACCTGCTTCAATTATGGGAATCGT
TGGTTTTAAACCATCATATGGTATTATTTCACGTTATGGAGTTTATCCTTTCGCTCCATCGCTTGATCATGTTGGTATTT
TCACAAATAATGTTTTAGATACTCAAATTGTTTTTAATGCCATAGCAAAACGTGATTTTAAAGATTTTACAAGTCTTGAT
TTTAAGACACAATCACTTACAACTCTAAAAACAAACAACAAATATAAAATTGGTATTTTTAAACCTAGTTTTGATTTATT
AGAGCCAAAATATCAACAAGAATTAAATAATTTAATTAATAAATTAAATAAAGACCATGAAGTTATTAGTGTTGATTATG
ATATTAATTTACTAAAAGCAATTAGTCCAGTTTATAAATTAGTTGCTTTTAGTGAAGCTTATAGTTCATACTCTAATTTT
ACAAATATTACTTTTGGTAAACACGATATAGAATATAAAAACTATGAAGATTTAATTATTAAAGCTCGAACTTTATATTT
AGGAACACAATTAAAACAACGTTTTATGATAGGTGCTTATGTTACTCAAGAAGCTCAATTTGAAACTTTATTAACTGCAG
CTAAAAAAATGCGAACATTAATTGTGCAAAAAGCTAAGCAGTTATTTACTAATTGTGATTTAATTTTAAACTTAGCAGTT
CATGATGTTAGCGAAACAATTGAAGATGTTAATAATCAAAAACCAACAACTAATTTAGTTGAAGATGTAATGCAAATTGC
TAATTTTGGTGGTTTTCCATCAATTGTAATTCCTTTTATAAAAACCTCATTAGGAAACTTAGGTTTAAATTTATGAGCAG
ATTATTTAAATGACAACAAACTATTAAATGCTGCTTATTTAATTAGTAATTTATTAGGAAATGAGGAGAAATAA

Upstream 100 bases:

>100_bases
TTCTTACGCCAAGACGAAATTGATTCTTGCGCAACTAATAATAGTGAAGAAGTTTTAAATAATTGTTTAGAAGTAATTGA
TCATTATGGGGTATTAAAAA

Downstream 100 bases:

>100_bases
TAATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTCCATACAGCACTTAATACTAAAACAAAAATGTTTTCTAATACT
CCTACATCTCATAAAAGTAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKNKTILTLHEQIKKREIKIYDLVKKTIDHNLKIKNLNASLANNYEQALNQASFLDQEISNNDTQIDYLFGIPYSLKDNI
STKNIITTGGSLFLENYQPPYDATVKTLLDQNQAILLNKSNLDEFGLGGTGTDSAFGIVVNPLDSTRVCGGSSSGSAVLV
QQGVVPFAIATDTGDSIRRPASIMGIVGFKPSYGIISRYGVYPFAPSLDHVGIFTNNVLDTQIVFNAIAKRDFKDFTSLD
FKTQSLTTLKTNNKYKIGIFKPSFDLLEPKYQQELNNLINKLNKDHEVISVDYDINLLKAISPVYKLVAFSEAYSSYSNF
TNITFGKHDIEYKNYEDLIIKARTLYLGTQLKQRFMIGAYVTQEAQFETLLTAAKKMRTLIVQKAKQLFTNCDLILNLAV
HDVSETIEDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFIKTSLGNLGLNLWADYLNDNKLLNAAYLISNLLGNEEK

Sequences:

>Translated_477_residues
MKNKTILTLHEQIKKREIKIYDLVKKTIDHNLKIKNLNASLANNYEQALNQASFLDQEISNNDTQIDYLFGIPYSLKDNI
STKNIITTGGSLFLENYQPPYDATVKTLLDQNQAILLNKSNLDEFGLGGTGTDSAFGIVVNPLDSTRVCGGSSSGSAVLV
QQGVVPFAIATDTGDSIRRPASIMGIVGFKPSYGIISRYGVYPFAPSLDHVGIFTNNVLDTQIVFNAIAKRDFKDFTSLD
FKTQSLTTLKTNNKYKIGIFKPSFDLLEPKYQQELNNLINKLNKDHEVISVDYDINLLKAISPVYKLVAFSEAYSSYSNF
TNITFGKHDIEYKNYEDLIIKARTLYLGTQLKQRFMIGAYVTQEAQFETLLTAAKKMRTLIVQKAKQLFTNCDLILNLAV
HDVSETIEDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFIKTSLGNLGLNL*ADYLNDNKLLNAAYLISNLLGNEEK
>Mature_477_residues
MKNKTILTLHEQIKKREIKIYDLVKKTIDHNLKIKNLNASLANNYEQALNQASFLDQEISNNDTQIDYLFGIPYSLKDNI
STKNIITTGGSLFLENYQPPYDATVKTLLDQNQAILLNKSNLDEFGLGGTGTDSAFGIVVNPLDSTRVCGGSSSGSAVLV
QQGVVPFAIATDTGDSIRRPASIMGIVGFKPSYGIISRYGVYPFAPSLDHVGIFTNNVLDTQIVFNAIAKRDFKDFTSLD
FKTQSLTTLKTNNKYKIGIFKPSFDLLEPKYQQELNNLINKLNKDHEVISVDYDINLLKAISPVYKLVAFSEAYSSYSNF
TNITFGKHDIEYKNYEDLIIKARTLYLGTQLKQRFMIGAYVTQEAQFETLLTAAKKMRTLIVQKAKQLFTNCDLILNLAV
HDVSETIEDVNNQKPTTNLVEDVMQIANFGGFPSIVIPFIKTSLGNLGLNL*ADYLNDNKLLNAAYLISNLLGNEEK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=485, Percent_Identity=30.7216494845361, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI195972892, Length=209, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=410, Percent_Identity=27.3170731707317, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17556264, Length=286, Percent_Identity=27.972027972028, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=149, Percent_Identity=36.241610738255, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=201, Percent_Identity=29.3532338308458, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=147, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=412, Percent_Identity=33.495145631068, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6319685, Length=254, Percent_Identity=33.0708661417323, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648113, Length=468, Percent_Identity=30.7692307692308, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI21356731, Length=204, Percent_Identity=31.3725490196078, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24644968, Length=211, Percent_Identity=29.8578199052133, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550774, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648435, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648437, Length=202, Percent_Identity=30.1980198019802, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24652985, Length=206, Percent_Identity=28.6407766990291, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922090, Length=206, Percent_Identity=28.6407766990291, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652981, Length=206, Percent_Identity=28.6407766990291, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24652983, Length=206, Percent_Identity=28.6407766990291, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161078093, Length=218, Percent_Identity=30.2752293577982, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=32.3741007194245, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=32.3741007194245, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53116; Mature: 53116

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKTILTLHEQIKKREIKIYDLVKKTIDHNLKIKNLNASLANNYEQALNQASFLDQEIS
CCCCEEEHHHHHHHHHCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
NNDTQIDYLFGIPYSLKDNISTKNIITTGGSLFLENYQPPYDATVKTLLDQNQAILLNKS
CCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCEEEEECC
NLDEFGLGGTGTDSAFGIVVNPLDSTRVCGGSSSGSAVLVQQGVVPFAIATDTGDSIRRP
CCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCEEEEEEECCCHHHHCC
ASIMGIVGFKPSYGIISRYGVYPFAPSLDHVGIFTNNVLDTQIVFNAIAKRDFKDFTSLD
HHHEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
FKTQSLTTLKTNNKYKIGIFKPSFDLLEPKYQQELNNLINKLNKDHEVISVDYDINLLKA
CCCCCEEEEECCCEEEEEEECCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
ISPVYKLVAFSEAYSSYSNFTNITFGKHDIEYKNYEDLIIKARTLYLGTQLKQRFMIGAY
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHH
VTQEAQFETLLTAAKKMRTLIVQKAKQLFTNCDLILNLAVHDVSETIEDVNNQKPTTNLV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EDVMQIANFGGFPSIVIPFIKTSLGNLGLNLADYLNDNKLLNAAYLISNLLGNEEK
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNKTILTLHEQIKKREIKIYDLVKKTIDHNLKIKNLNASLANNYEQALNQASFLDQEIS
CCCCEEEHHHHHHHHHCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHC
NNDTQIDYLFGIPYSLKDNISTKNIITTGGSLFLENYQPPYDATVKTLLDQNQAILLNKS
CCCCEEEEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCEEEEECC
NLDEFGLGGTGTDSAFGIVVNPLDSTRVCGGSSSGSAVLVQQGVVPFAIATDTGDSIRRP
CCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCEEEEECCCEEEEEEECCCHHHHCC
ASIMGIVGFKPSYGIISRYGVYPFAPSLDHVGIFTNNVLDTQIVFNAIAKRDFKDFTSLD
HHHEEEECCCCCCCCHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
FKTQSLTTLKTNNKYKIGIFKPSFDLLEPKYQQELNNLINKLNKDHEVISVDYDINLLKA
CCCCCEEEEECCCEEEEEEECCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHH
ISPVYKLVAFSEAYSSYSNFTNITFGKHDIEYKNYEDLIIKARTLYLGTQLKQRFMIGAY
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHH
VTQEAQFETLLTAAKKMRTLIVQKAKQLFTNCDLILNLAVHDVSETIEDVNNQKPTTNLV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EDVMQIANFGGFPSIVIPFIKTSLGNLGLNLADYLNDNKLLNAAYLISNLLGNEEK
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]