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The map label for this gene is gatB [H]

Identifier: 209554532

GI number: 209554532

Start: 761184

End: 762617

Strand: Reverse

Name: gatB [H]

Synonym: UUR10_0635

Alternate gene names: 209554532

Gene position: 762617-761184 (Counterclockwise)

Preceding gene: 209554383

Following gene: 209554483

Centisome position: 87.21

GC content: 26.92

Gene sequence:

>1434_bases
ATGCAAAATTTTGAAGTTATTATTGGTATTGAAGTCCATACAGCACTTAATACTAAAACAAAAATGTTTTCTAATACTCC
TACATCTCATAAAAGTATGGCTAATACTTTGATTAATGAAATTGATTTAGCATTACCCGGAACATTACCAAGTGTTAATC
AAGAAGTTGTACATAAAGGCCTTTTTTTAGCAAACGCTTTACACATGCATACAAATCATCAATTTATTGCTTTTGATCGT
AAACATTATTACTATTTAGACTTACCTAAGGGTTATCAAATTACGCAAAATTATTTTCCTATTGGTCAAAATGGTTATAT
TCAAATCACTGATGAAAATAATAATCCAAAAAAAATACGTATTAAACAAATTCATTTAGAAGAAGATACCGCTAAACAAA
CTAGTGTTAATAATCAAGTTTATTTAGATTATAATCGTGCTGGTTGACCACTAATTGAAATTGTAAGCGAAGCTGATTTA
AGAAGTGCACAAGAGACTGTTTTGTTTTTAGAAGAATTACGTAAGATTTTATTATTTAATGATATATCAGATGCAAAAAT
GGAAGATGGTTCATTACGTGTTGATGTAAATATTTCAATTAGACCACGGGGTGCAAAATCATTTGGTACAAAAGTTGAAA
TTAAGAATATTAACTCTATTTCTAATGTTGCTAAAGCGATTAATTACGAGTATAATCGTCAATTAAATTTAATTTTATTA
AACCAAAGTGTTGAACAACAAACACGTCGTTTTGATGATAGTACTAATACAACTGTTTTTATGCGTTCAAAAAACGATGC
CATTAATTATCGTTATATTCGAGAACTAAATATTGCGCCAATTTATTTAAGTGATGAATATGTTAGTCAATTATTATCTA
CTAAACCTTATAGTATTAATGATTTACGTCAAGAATTATTACAAAAAGGCTTAGTTAGCTCAGCTATTGAACAACTATTG
GGTGATGGCCCTTTATTTAAAGCGTTTAAATATGTCAATAAGATTGTTAACAATCCTTCAAGTGTTTATAAATGACTATG
TTTAGAATTCATTGGTTTAATTAATAAGAACACTCAAATCATTGAAGATATTTCAATGGAATTATTACAAAAAATTGGCG
CCATGATTGTATTATTTGATCAAACTTTAATAAATGGGAAACAAACAAAAACAATTTTAGAAAAAATTTATTTAACAAAT
AAAGACCCACAAACACTAATTAAAGAATTAGGATTTGAACAAATTACTGATGAAAATGAAATTACTAACCTTTGAAATCA
AATCCTAGCTAATAACCAAGAAATGTTATTACAATATGAAGAACGACCCGATCGAGTTGAAAAATTCTTTATGGGTGAAA
TGATGAAATTAACTAAAGCACAAGCTAATCCAACAATTTCTTTCAACATTTTAAAAAAAATATTACAAAAATAA

Upstream 100 bases:

>100_bases
ACTTAGGTTTAAATTTATGAGCAGATTATTTAAATGACAACAAACTATTAAATGCTGCTTATTTAATTAGTAATTTATTA
GGAAATGAGGAGAAATAATA

Downstream 100 bases:

>100_bases
AAAAACATTGTATAATTATTTTTATACTAAGAAGTTTGCAAATACTTTTTAGTTGATATTATGTATAGAATTAAAAAAGC
TCGTGGTTTTCGCAAATTAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MQNFEVIIGIEVHTALNTKTKMFSNTPTSHKSMANTLINEIDLALPGTLPSVNQEVVHKGLFLANALHMHTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQITDENNNPKKIRIKQIHLEEDTAKQTSVNNQVYLDYNRAGWPLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNISIRPRGAKSFGTKVEIKNINSISNVAKAINYEYNRQLNLILL
NQSVEQQTRRFDDSTNTTVFMRSKNDAINYRYIRELNIAPIYLSDEYVSQLLSTKPYSINDLRQELLQKGLVSSAIEQLL
GDGPLFKAFKYVNKIVNNPSSVYKWLCLEFIGLINKNTQIIEDISMELLQKIGAMIVLFDQTLINGKQTKTILEKIYLTN
KDPQTLIKELGFEQITDENEITNLWNQILANNQEMLLQYEERPDRVEKFFMGEMMKLTKAQANPTISFNILKKILQK

Sequences:

>Translated_477_residues
MQNFEVIIGIEVHTALNTKTKMFSNTPTSHKSMANTLINEIDLALPGTLPSVNQEVVHKGLFLANALHMHTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQITDENNNPKKIRIKQIHLEEDTAKQTSVNNQVYLDYNRAG*PLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNISIRPRGAKSFGTKVEIKNINSISNVAKAINYEYNRQLNLILL
NQSVEQQTRRFDDSTNTTVFMRSKNDAINYRYIRELNIAPIYLSDEYVSQLLSTKPYSINDLRQELLQKGLVSSAIEQLL
GDGPLFKAFKYVNKIVNNPSSVYK*LCLEFIGLINKNTQIIEDISMELLQKIGAMIVLFDQTLINGKQTKTILEKIYLTN
KDPQTLIKELGFEQITDENEITNL*NQILANNQEMLLQYEERPDRVEKFFMGEMMKLTKAQANPTISFNILKKILQK
>Mature_477_residues
MQNFEVIIGIEVHTALNTKTKMFSNTPTSHKSMANTLINEIDLALPGTLPSVNQEVVHKGLFLANALHMHTNHQFIAFDR
KHYYYLDLPKGYQITQNYFPIGQNGYIQITDENNNPKKIRIKQIHLEEDTAKQTSVNNQVYLDYNRAG*PLIEIVSEADL
RSAQETVLFLEELRKILLFNDISDAKMEDGSLRVDVNISIRPRGAKSFGTKVEIKNINSISNVAKAINYEYNRQLNLILL
NQSVEQQTRRFDDSTNTTVFMRSKNDAINYRYIRELNIAPIYLSDEYVSQLLSTKPYSINDLRQELLQKGLVSSAIEQLL
GDGPLFKAFKYVNKIVNNPSSVYK*LCLEFIGLINKNTQIIEDISMELLQKIGAMIVLFDQTLINGKQTKTILEKIYLTN
KDPQTLIKELGFEQITDENEITNL*NQILANNQEMLLQYEERPDRVEKFFMGEMMKLTKAQANPTISFNILKKILQK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=30.4435483870968, Blast_Score=217, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI115534094, Length=303, Percent_Identity=39.2739273927393, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=320, Percent_Identity=34.6875, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI45550808, Length=500, Percent_Identity=30.2, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54416; Mature: 54416

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFEVIIGIEVHTALNTKTKMFSNTPTSHKSMANTLINEIDLALPGTLPSVNQEVVHKG
CCCCEEEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LFLANALHMHTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQITDENNNPKKIR
HHHHHHHHCCCCCEEEEEECCEEEEEECCCCCEEECCCCCCCCCCEEEEEECCCCCCEEE
IKQIHLEEDTAKQTSVNNQVYLDYNRAGPLIEIVSEADLRSAQETVLFLEELRKILLFND
EEEEEECCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISDAKMEDGSLRVDVNISIRPRGAKSFGTKVEIKNINSISNVAKAINYEYNRQLNLILLN
CCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEEC
QSVEQQTRRFDDSTNTTVFMRSKNDAINYRYIRELNIAPIYLSDEYVSQLLSTKPYSIND
CHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCCCHHH
LRQELLQKGLVSSAIEQLLGDGPLFKAFKYVNKIVNNPSSVYKLCLEFIGLINKNTQIIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
DISMELLQKIGAMIVLFDQTLINGKQTKTILEKIYLTNKDPQTLIKELGFEQITDENEIT
HHHHHHHHHHCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHCCCCCHHH
NLNQILANNQEMLLQYEERPDRVEKFFMGEMMKLTKAQANPTISFNILKKILQK
HHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MQNFEVIIGIEVHTALNTKTKMFSNTPTSHKSMANTLINEIDLALPGTLPSVNQEVVHKG
CCCCEEEEEEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LFLANALHMHTNHQFIAFDRKHYYYLDLPKGYQITQNYFPIGQNGYIQITDENNNPKKIR
HHHHHHHHCCCCCEEEEEECCEEEEEECCCCCEEECCCCCCCCCCEEEEEECCCCCCEEE
IKQIHLEEDTAKQTSVNNQVYLDYNRAGPLIEIVSEADLRSAQETVLFLEELRKILLFND
EEEEEECCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ISDAKMEDGSLRVDVNISIRPRGAKSFGTKVEIKNINSISNVAKAINYEYNRQLNLILLN
CCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEEC
QSVEQQTRRFDDSTNTTVFMRSKNDAINYRYIRELNIAPIYLSDEYVSQLLSTKPYSIND
CHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCCCHHH
LRQELLQKGLVSSAIEQLLGDGPLFKAFKYVNKIVNNPSSVYKLCLEFIGLINKNTQIIE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHH
DISMELLQKIGAMIVLFDQTLINGKQTKTILEKIYLTNKDPQTLIKELGFEQITDENEIT
HHHHHHHHHHCCEEEEEEHHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCHHHCCCCCHHH
NLNQILANNQEMLLQYEERPDRVEKFFMGEMMKLTKAQANPTISFNILKKILQK
HHHHHHHCCHHHEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA