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The map label for this gene is thrS [H]

Identifier: 209554092

GI number: 209554092

Start: 745561

End: 747303

Strand: Reverse

Name: thrS [H]

Synonym: UUR10_0626

Alternate gene names: 209554092

Gene position: 747303-745561 (Counterclockwise)

Preceding gene: 209554225

Following gene: 209554057

Centisome position: 85.46

GC content: 28.28

Gene sequence:

>1743_bases
ATGTATAAGTTTGATCCAAAACTAAATCATAGTGCTGCGCATGTTTTAGCAATGGCACTAACAAAATTTTATCCTAACTT
AAGTCTTGCAATTGGTCCAGCTATTGATGAGGGCTTTTATTATGATTTTAATTTAAATGATCCAAATACCTCAATCACCC
CTATCGATTTATTGAAAATTGAAAAAGAAATGAAAAAAATTGCTGCTCAAGCATTAACTTTTGACTATGAACAAGTTAGC
TATGAACAAGCAAAAGATTTATTTAAAAATAACAAATACAAATTAGACATTATTGAAGAAAATAAGAATAAACCACTTTC
AATTTATCATTCAGGTAAATGATTTGATTTGTGTAAAGGTCCTCATGTTGAAAATACAAAAGAAATTAAAGCAATTAAGT
TATTAAATATTGCTGGTTCATATTGACGAGGCGATGCAAATAATGATCAACTAATTCGTATTTATGGTGTTGCTTTTAAT
GACCAAGCCCAATTAGATGCTTATTTAGTTGATTTACAAGAACGTAAAGAACGTGATCATCGTAAAATTGGTAAAGATTT
AAATTTATTTACTTTTAATAATTTAGCTGGTCAAGGTTTACCAATCTGATTACCAAATGGAACAATTATTAAAAGTCAAG
TTCAAAAATTTATTAATGAAGTTGAATTTCAATTTAATTTTGACACTGTTATTACGCCAATATTAGGAAGTATTGATTTA
TATAAAACAAGTGGTCATTGAGATCATTATAAAGACAATATTTTTTCACCAGTTCAAATTGATAATGAAGTATTAATACT
TCGTCCAATGACATGTCCCCATCATACATTAGTTTATTCTAATGAACTACGTTCATATCGTAGTTTACCAATTCGTTTAA
GTGAACATTCAATTTTACATCGTTATGAATCATCTGGTGGATTAACTGGTTTTGAACGAGTGCGTGAAATGATTTTAGAA
GATTGCCATGTATTCTGTCGTCCTGATCAGATTGAACATGAAGTAATTAATGCTTTTAAAATGATTCAAGAAGCACAAGA
AGGATTAGGTATTAAAACATTTGAAATTCATTTATCTTTAAATGATCCAAATGATAAAGAAAAATACTATGATGATCCTC
AAATGTGAGAACATTCACAAAATGCATTACGTAAAATGTTAAAAGATCATAAAATTCCTTACAAAGAAATGGTCGGCGAA
GCAGCATTTTATGGGCCAAAAATTGATTTCCAAGTTAAAACAGTTTTAAATCGAATCATTACAGTTTCAACTATTCAATT
AGACTTTTTATTACCAAATCGTTTTAATTTAAGTTATATTAATGAAAACAATGAACAAAGCACACCAGTAATGATTCATA
TTGGAATCATAGGTACTTATGAACGTTTATTAGCGATCTTATTAGAACAAACTAAAGGTGTTTTACCACTTTGATTATCA
CCTGTTCAAGTAGTTATTATTCCTGTTAATGAAAATTTACATGCTGATTATGCAAAAGAATTAAATGCAAAATTACGAAA
ACACTTAGTACGTAGTAGTTTAGATTTACGTAATGAACGTTTATCAAAGAAAATTCGTGAGGCACAAATTCAAAAAATTC
CATATCAAATTGTTGTTGGAGATGAAGAAATTAAAAACGATAAAATGGTAACTTATCGTCGTTATGGTAGTGAAGAAACA
ACAACAGTTTCTGTGGATGATTTTATAAAGGTGTTAGAAAACGACATCAGATCAAAAAAATAA

Upstream 100 bases:

>100_bases
AGCAAAATATGGATTTTATAGACTTATTATCTTTATGTAAAGTTATATAATATATTAATGAAAGAAAATAATTATTTAAA
AATATTTTATCGAGGTATTT

Downstream 100 bases:

>100_bases
ATTAAATAATGTTATTTTTAATATAATTAATAGATTATTGTTCGAAGAGGTGAATATATAATGGCGATTAAACGAGGCGT
TCGATTGCAATGCAATGAGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MYKFDPKLNHSAAHVLAMALTKFYPNLSLAIGPAIDEGFYYDFNLNDPNTSITPIDLLKIEKEMKKIAAQALTFDYEQVS
YEQAKDLFKNNKYKLDIIEENKNKPLSIYHSGKWFDLCKGPHVENTKEIKAIKLLNIAGSYWRGDANNDQLIRIYGVAFN
DQAQLDAYLVDLQERKERDHRKIGKDLNLFTFNNLAGQGLPIWLPNGTIIKSQVQKFINEVEFQFNFDTVITPILGSIDL
YKTSGHWDHYKDNIFSPVQIDNEVLILRPMTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYESSGGLTGFERVREMILE
DCHVFCRPDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKEKYYDDPQMWEHSQNALRKMLKDHKIPYKEMVGE
AAFYGPKIDFQVKTVLNRIITVSTIQLDFLLPNRFNLSYINENNEQSTPVMIHIGIIGTYERLLAILLEQTKGVLPLWLS
PVQVVIIPVNENLHADYAKELNAKLRKHLVRSSLDLRNERLSKKIREAQIQKIPYQIVVGDEEIKNDKMVTYRRYGSEET
TTVSVDDFIKVLENDIRSKK

Sequences:

>Translated_580_residues
MYKFDPKLNHSAAHVLAMALTKFYPNLSLAIGPAIDEGFYYDFNLNDPNTSITPIDLLKIEKEMKKIAAQALTFDYEQVS
YEQAKDLFKNNKYKLDIIEENKNKPLSIYHSGK*FDLCKGPHVENTKEIKAIKLLNIAGSY*RGDANNDQLIRIYGVAFN
DQAQLDAYLVDLQERKERDHRKIGKDLNLFTFNNLAGQGLPI*LPNGTIIKSQVQKFINEVEFQFNFDTVITPILGSIDL
YKTSGH*DHYKDNIFSPVQIDNEVLILRPMTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYESSGGLTGFERVREMILE
DCHVFCRPDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKEKYYDDPQM*EHSQNALRKMLKDHKIPYKEMVGE
AAFYGPKIDFQVKTVLNRIITVSTIQLDFLLPNRFNLSYINENNEQSTPVMIHIGIIGTYERLLAILLEQTKGVLPL*LS
PVQVVIIPVNENLHADYAKELNAKLRKHLVRSSLDLRNERLSKKIREAQIQKIPYQIVVGDEEIKNDKMVTYRRYGSEET
TTVSVDDFIKVLENDIRSKK
>Mature_580_residues
MYKFDPKLNHSAAHVLAMALTKFYPNLSLAIGPAIDEGFYYDFNLNDPNTSITPIDLLKIEKEMKKIAAQALTFDYEQVS
YEQAKDLFKNNKYKLDIIEENKNKPLSIYHSGK*FDLCKGPHVENTKEIKAIKLLNIAGSY*RGDANNDQLIRIYGVAFN
DQAQLDAYLVDLQERKERDHRKIGKDLNLFTFNNLAGQGLPI*LPNGTIIKSQVQKFINEVEFQFNFDTVITPILGSIDL
YKTSGH*DHYKDNIFSPVQIDNEVLILRPMTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYESSGGLTGFERVREMILE
DCHVFCRPDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPNDKEKYYDDPQM*EHSQNALRKMLKDHKIPYKEMVGE
AAFYGPKIDFQVKTVLNRIITVSTIQLDFLLPNRFNLSYINENNEQSTPVMIHIGIIGTYERLLAILLEQTKGVLPL*LS
PVQVVIIPVNENLHADYAKELNAKLRKHLVRSSLDLRNERLSKKIREAQIQKIPYQIVVGDEEIKNDKMVTYRRYGSEET
TTVSVDDFIKVLENDIRSKK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=546, Percent_Identity=36.4468864468864, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI38202255, Length=536, Percent_Identity=36.5671641791045, Blast_Score=336, Evalue=4e-92,
Organism=Homo sapiens, GI20070344, Length=551, Percent_Identity=35.934664246824, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI1788013, Length=574, Percent_Identity=36.2369337979094, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71984184, Length=542, Percent_Identity=35.4243542435424, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71984192, Length=542, Percent_Identity=35.4243542435424, Blast_Score=325, Evalue=3e-89,
Organism=Saccharomyces cerevisiae, GI6322112, Length=581, Percent_Identity=35.6282271944923, Blast_Score=339, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6322654, Length=381, Percent_Identity=34.6456692913386, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24583842, Length=565, Percent_Identity=35.7522123893805, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI28574141, Length=565, Percent_Identity=35.7522123893805, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24583839, Length=565, Percent_Identity=35.7522123893805, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 66083; Mature: 66083

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKFDPKLNHSAAHVLAMALTKFYPNLSLAIGPAIDEGFYYDFNLNDPNTSITPIDLLKI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHH
EKEMKKIAAQALTFDYEQVSYEQAKDLFKNNKYKLDIIEENKNKPLSIYHSGKFDLCKGP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCC
HVENTKEIKAIKLLNIAGSYRGDANNDQLIRIYGVAFNDQAQLDAYLVDLQERKERDHRK
CCCCCHHEEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IGKDLNLFTFNNLAGQGLPILPNGTIIKSQVQKFINEVEFQFNFDTVITPILGSIDLYKT
HCCCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHEEEECCHHHHHHHHCCEEEEEC
SGHDHYKDNIFSPVQIDNEVLILRPMTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYES
CCCCCHHHCCCCCEEECCCEEEEECCCCCCCEEEECHHHHHHHCCCEEECCHHHHHHHHC
SGGLTGFERVREMILEDCHVFCRPDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPN
CCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
DKEKYYDDPQMEHSQNALRKMLKDHKIPYKEMVGEAAFYGPKIDFQVKTVLNRIITVSTI
CCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCCEEHHHHHHHHEEEEEE
QLDFLLPNRFNLSYINENNEQSTPVMIHIGIIGTYERLLAILLEQTKGVLPLLSPVQVVI
EEEEECCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPVNENLHADYAKELNAKLRKHLVRSSLDLRNERLSKKIREAQIQKIPYQIVVGDEEIKN
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCC
DKMVTYRRYGSEETTTVSVDDFIKVLENDIRSKK
CCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYKFDPKLNHSAAHVLAMALTKFYPNLSLAIGPAIDEGFYYDFNLNDPNTSITPIDLLKI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHH
EKEMKKIAAQALTFDYEQVSYEQAKDLFKNNKYKLDIIEENKNKPLSIYHSGKFDLCKGP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCC
HVENTKEIKAIKLLNIAGSYRGDANNDQLIRIYGVAFNDQAQLDAYLVDLQERKERDHRK
CCCCCHHEEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IGKDLNLFTFNNLAGQGLPILPNGTIIKSQVQKFINEVEFQFNFDTVITPILGSIDLYKT
HCCCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHEEEECCHHHHHHHHCCEEEEEC
SGHDHYKDNIFSPVQIDNEVLILRPMTCPHHTLVYSNELRSYRSLPIRLSEHSILHRYES
CCCCCHHHCCCCCEEECCCEEEEECCCCCCCEEEECHHHHHHHCCCEEECCHHHHHHHHC
SGGLTGFERVREMILEDCHVFCRPDQIEHEVINAFKMIQEAQEGLGIKTFEIHLSLNDPN
CCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCC
DKEKYYDDPQMEHSQNALRKMLKDHKIPYKEMVGEAAFYGPKIDFQVKTVLNRIITVSTI
CCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCHHHCCCCCCEEHHHHHHHHEEEEEE
QLDFLLPNRFNLSYINENNEQSTPVMIHIGIIGTYERLLAILLEQTKGVLPLLSPVQVVI
EEEEECCCCCEEEEECCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IPVNENLHADYAKELNAKLRKHLVRSSLDLRNERLSKKIREAQIQKIPYQIVVGDEEIKN
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCC
DKMVTYRRYGSEETTTVSVDDFIKVLENDIRSKK
CCEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA