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The map label for this gene is truA [H]

Identifier: 209554225

GI number: 209554225

Start: 748353

End: 749078

Strand: Reverse

Name: truA [H]

Synonym: UUR10_0627

Alternate gene names: 209554225

Gene position: 749078-748353 (Counterclockwise)

Preceding gene: 209554345

Following gene: 209554092

Centisome position: 85.66

GC content: 25.48

Gene sequence:

>726_bases
ATGAATTATAAAATTAGTATTCGTTATGATGGAAGTTTATTTTATGGATGAGCTAAACAACCACAAAAAAGAACCGTGCA
AGAACATTTAGAAACTGTTTTTAAAACTATTTTTAAAATTAATGATATTAAAATCATTGGCTCTGGACGCACTGATAGGG
GCGTACATGCTTATGAACAAGTTTTTAGTGTTAATCATCATCAACTAAATTATGAACCACAAGTTATTTATCAAGCGCTT
TGTTCACAAATTGATCCTGATGTTCAAATTTTAAAAGTTCAAAGTGTTCATGATACATTCCATGCACAACATGATGCGAT
TTCAAAAACATATCAATATATTATTAATGATTATGAATTTGATTTATTCAAACACAATTATGAATATTTTATTCATCAAA
AAATTAATGATCAAAAACTATTAGAAGCCTTAGAATTATTTGTTGGTGAGTATGATTTTAAATCATTTTCAACAAGTGAA
TTACCACTAACAACACGAAAAATTAATTGAGTTAAAATTACAAGAAATACTCGTTTAGAAATTTATATTAATGGCAATGG
CTTTTTAAAAAATATGGTACGAATGATCATTAGTGCTTGTATTGATTATGTTTTTAATAAAATTAGTTTAACTCAAATAA
AAACATTATTAACTAATCCTAAAAAAGGTGCTAGCGTTAAACTAGCACCTCCTTGTGGTTTATATTTATATAAAGTTTAT
TATTAA

Upstream 100 bases:

>100_bases
TTCGGCGCTTTTGAAGCGAGTGCGCTATTTGCTAAATAGATTTGATAAAATAACTTGTTGAGCAAGTTATTTTTTTATTA
TTAGATAAGGATTAATAGTC

Downstream 100 bases:

>100_bases
TGTTTTTTTTCAACATGTTTTTTAGGAATATTGGCCATTACTTGAATACCAAGCGCTCCTTTTCAAACATATACTAAAAA
GATTGGTGGACCAGAACGAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNYKISIRYDGSLFYGWAKQPQKRTVQEHLETVFKTIFKINDIKIIGSGRTDRGVHAYEQVFSVNHHQLNYEPQVIYQAL
CSQIDPDVQILKVQSVHDTFHAQHDAISKTYQYIINDYEFDLFKHNYEYFIHQKINDQKLLEALELFVGEYDFKSFSTSE
LPLTTRKINWVKITRNTRLEIYINGNGFLKNMVRMIISACIDYVFNKISLTQIKTLLTNPKKGASVKLAPPCGLYLYKVY
Y

Sequences:

>Translated_241_residues
MNYKISIRYDGSLFYG*AKQPQKRTVQEHLETVFKTIFKINDIKIIGSGRTDRGVHAYEQVFSVNHHQLNYEPQVIYQAL
CSQIDPDVQILKVQSVHDTFHAQHDAISKTYQYIINDYEFDLFKHNYEYFIHQKINDQKLLEALELFVGEYDFKSFSTSE
LPLTTRKIN*VKITRNTRLEIYINGNGFLKNMVRMIISACIDYVFNKISLTQIKTLLTNPKKGASVKLAPPCGLYLYKVY
Y
>Mature_241_residues
MNYKISIRYDGSLFYG*AKQPQKRTVQEHLETVFKTIFKINDIKIIGSGRTDRGVHAYEQVFSVNHHQLNYEPQVIYQAL
CSQIDPDVQILKVQSVHDTFHAQHDAISKTYQYIINDYEFDLFKHNYEYFIHQKINDQKLLEALELFVGEYDFKSFSTSE
LPLTTRKIN*VKITRNTRLEIYINGNGFLKNMVRMIISACIDYVFNKISLTQIKTLLTNPKKGASVKLAPPCGLYLYKVY
Y

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=245, Percent_Identity=24.0816326530612, Blast_Score=78, Evalue=6e-15,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=28.8617886178862, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI116007752, Length=299, Percent_Identity=27.0903010033445, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27936; Mature: 27936

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKISIRYDGSLFYGAKQPQKRTVQEHLETVFKTIFKINDIKIIGSGRTDRGVHAYEQV
CCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
FSVNHHQLNYEPQVIYQALCSQIDPDVQILKVQSVHDTFHAQHDAISKTYQYIINDYEFD
HHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEE
LFKHNYEYFIHQKINDQKLLEALELFVGEYDFKSFSTSELPLTTRKINVKITRNTRLEIY
EECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCEEEEE
INGNGFLKNMVRMIISACIDYVFNKISLTQIKTLLTNPKKGASVKLAPPCGLYLYKVYY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEC
>Mature Secondary Structure
MNYKISIRYDGSLFYGAKQPQKRTVQEHLETVFKTIFKINDIKIIGSGRTDRGVHAYEQV
CCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
FSVNHHQLNYEPQVIYQALCSQIDPDVQILKVQSVHDTFHAQHDAISKTYQYIINDYEFD
HHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEE
LFKHNYEYFIHQKINDQKLLEALELFVGEYDFKSFSTSELPLTTRKINVKITRNTRLEIY
EECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCEEEEE
INGNGFLKNMVRMIISACIDYVFNKISLTQIKTLLTNPKKGASVKLAPPCGLYLYKVYY
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA