Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 209551904

GI number: 209551904

Start: 4502497

End: 4503444

Strand: Direct

Name: gshB [H]

Synonym: Rleg2_4337

Alternate gene names: 209551904

Gene position: 4502497-4503444 (Clockwise)

Preceding gene: 209551903

Following gene: 209551905

Centisome position: 99.22

GC content: 62.87

Gene sequence:

>948_bases
ATGGCCAAGATCACCAATGTAGCGGTCCAGATGGATCATGTCGCCGGCATCAATATTGCGGGCGACTCCACCTTCGCCAT
GAGCCTCGAAGCCCAGGCGCGCGGCTACCGGCTGTTTCATTATACGCCTGAGCGGCTGAGCTTCCGCGACGGCAAGCTCT
ATGCCAGCGTCGAGCCGATGCTGCTGCGCGACGTCAAGGGCGATCACTTCGAGCTTGGCGCGCCTGAGCGCGTCGATCTT
TCCACCATGGACGTCGTGCTGCTGCGCCAGGACCCGCCCTTCGACATGGCCTATATCACCTCGACGCATCTGCTCGAGCG
CATCCATCCGAAGACGCTGGTAGTCAACGATCCGGCCTGGGTGCGCAATTCGCCCGAAAAGATCTTCGTCACCGAATTTT
CCGACCTGATGCCGAAGACGCTGATCACCAAGGATGCCGCCGAGATCCGCCGCTTCCGGGACGAGATGGGCGATATCATC
CTGAAGCCGCTCTACGGCAATGGCGGCGCAGGCGTCTTCCATTCGACCCGCGACGACCGCAACCTCTCCTCGCTGCTGGA
AATGTTCGGCCAGCTCTTCCGCGAGCCCTTCATCGCCCAGCAATATCTGCCTGACGTGCGCAAGGGCGACAAGCGCATCA
TCCTGGTCGACGGCGAATTCGCCGGCGCCATCAACCGCGTCCCGGCCGAACATGACAGCCGCTCCAACATGCATGTCGGC
GGCCGCGCCGAGGCGACCGAGCTGACGCCGCGCGAGAAGGAAATCTGCGAACGGATCGGCCCGGCGCTGCGTGAGCGCGG
CTTCCTGCTCGTCGGCATCGACGTGATCGGCGATTACATGACCGAGATCAACGTCACGTCGCCGACAGGCATCCGCGAAG
TCAAGAAGTTCGGTGGCGCCGATATCGCAAGCCTGCTCTGGGATGCGATCGAGCGCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
TCGAAACACGGTAATTGGGTGGCGGCGGCAAGCCTTGATTGACCGTTGCGTTTGATACCCGGCCGACCTACATCTGTCCG
GCATTCAGCGAGGGACGGAA

Downstream 100 bases:

>100_bases
GCCTGAGCCTGGTGCTCCGCGACCCCACGCAGCCACTGTGACCGGGTACAACCGCGGTCACAGCCTATATCTGACTCCGT
TCTTTTCTTGTTCTTGTTTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAKITNVAVQMDHVAGINIAGDSTFAMSLEAQARGYRLFHYTPERLSFRDGKLYASVEPMLLRDVKGDHFELGAPERVDL
STMDVVLLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFSDLMPKTLITKDAAEIRRFRDEMGDII
LKPLYGNGGAGVFHSTRDDRNLSSLLEMFGQLFREPFIAQQYLPDVRKGDKRIILVDGEFAGAINRVPAEHDSRSNMHVG
GRAEATELTPREKEICERIGPALRERGFLLVGIDVIGDYMTEINVTSPTGIREVKKFGGADIASLLWDAIERKRG

Sequences:

>Translated_315_residues
MAKITNVAVQMDHVAGINIAGDSTFAMSLEAQARGYRLFHYTPERLSFRDGKLYASVEPMLLRDVKGDHFELGAPERVDL
STMDVVLLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFSDLMPKTLITKDAAEIRRFRDEMGDII
LKPLYGNGGAGVFHSTRDDRNLSSLLEMFGQLFREPFIAQQYLPDVRKGDKRIILVDGEFAGAINRVPAEHDSRSNMHVG
GRAEATELTPREKEICERIGPALRERGFLLVGIDVIGDYMTEINVTSPTGIREVKKFGGADIASLLWDAIERKRG
>Mature_314_residues
AKITNVAVQMDHVAGINIAGDSTFAMSLEAQARGYRLFHYTPERLSFRDGKLYASVEPMLLRDVKGDHFELGAPERVDLS
TMDVVLLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAWVRNSPEKIFVTEFSDLMPKTLITKDAAEIRRFRDEMGDIIL
KPLYGNGGAGVFHSTRDDRNLSSLLEMFGQLFREPFIAQQYLPDVRKGDKRIILVDGEFAGAINRVPAEHDSRSNMHVGG
RAEATELTPREKEICERIGPALRERGFLLVGIDVIGDYMTEINVTSPTGIREVKKFGGADIASLLWDAIERKRG

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=312, Percent_Identity=41.025641025641, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1787076, Length=159, Percent_Identity=32.0754716981132, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35386; Mature: 35255

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITNVAVQMDHVAGINIAGDSTFAMSLEAQARGYRLFHYTPERLSFRDGKLYASVEPM
CCCEEEEEEEEECCCEEEECCCCEEEEEEECCCCCEEEEEECHHHCCCCCCEEEEECCHH
LLRDVKGDHFELGAPERVDLSTMDVVLLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAW
HHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFSDLMPKTLITKDAAEIRRFRDEMGDIILKPLYGNGGAGVFHSTRDDR
HCCCCCEEEEECHHHHCCHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCC
NLSSLLEMFGQLFREPFIAQQYLPDVRKGDKRIILVDGEFAGAINRVPAEHDSRSNMHVG
CHHHHHHHHHHHHHCHHHHHHHCCHHHCCCCEEEEECCCHHCHHHCCCCCCCCCCCCEEC
GRAEATELTPREKEICERIGPALRERGFLLVGIDVIGDYMTEINVTSPTGIREVKKFGGA
CCCCCCCCCCCHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCC
DIASLLWDAIERKRG
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKITNVAVQMDHVAGINIAGDSTFAMSLEAQARGYRLFHYTPERLSFRDGKLYASVEPM
CCEEEEEEEEECCCEEEECCCCEEEEEEECCCCCEEEEEECHHHCCCCCCEEEEECCHH
LLRDVKGDHFELGAPERVDLSTMDVVLLRQDPPFDMAYITSTHLLERIHPKTLVVNDPAW
HHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHH
VRNSPEKIFVTEFSDLMPKTLITKDAAEIRRFRDEMGDIILKPLYGNGGAGVFHSTRDDR
HCCCCCEEEEECHHHHCCHHHHHCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCC
NLSSLLEMFGQLFREPFIAQQYLPDVRKGDKRIILVDGEFAGAINRVPAEHDSRSNMHVG
CHHHHHHHHHHHHHCHHHHHHHCCHHHCCCCEEEEECCCHHCHHHCCCCCCCCCCCCEEC
GRAEATELTPREKEICERIGPALRERGFLLVGIDVIGDYMTEINVTSPTGIREVKKFGGA
CCCCCCCCCCCHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCC
DIASLLWDAIERKRG
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]