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The map label for this gene is lepA [H]

Identifier: 209551823

GI number: 209551823

Start: 4427881

End: 4429713

Strand: Reverse

Name: lepA [H]

Synonym: Rleg2_4255

Alternate gene names: 209551823

Gene position: 4429713-4427881 (Counterclockwise)

Preceding gene: 209551824

Following gene: 209551822

Centisome position: 97.61

GC content: 63.01

Gene sequence:

>1833_bases
ATGGCGCGCATGAGCACCAATTCGACCACTCCGCTGTCCCATATCCGCAACTTTTCGATCGTGGCCCACATCGACCACGG
CAAATCGACGCTGGCCGACCGGCTGATCCAGACGACGGGCGGCCTTGCCGAGCGCGAAATGTCCGAGCAGGTGCTCGACA
ATATGGATATCGAGCGCGAGCGCGGCATCACCATCAAGGCCCAGACGGTGCGCCTGCATTACCAGGCGAACAATGGCGAG
AAATACATCCTCAACCTGATCGACACACCCGGCCACGTCGACTTCGCCTATGAAGTGTCGCGGTCGCTGTCGGCCTGCGA
AGGCTCGCTGCTCGTCGTCGACGCCAGCCAGGGCGTGGAAGCGCAGACGCTCGCCAACGTCTACCAGGCGATCGACAACA
ATCACGAACTCGTCACCGTGCTCAACAAGATCGACCTGCCGGCCGCCGAACCCGACCGCATCAAGGAACAGATCGAGGAA
GTGATCGGCATCGACGCTTCGGAGGCAGTACTGATCTCGGCCAAAACCGGCCTCGGCATTCCCGACGTGCTGGAGGCGAT
CGTCAACAGGCTGCCGGCCCCGAAGAGCCCGGGCGGCGACAAGGCGCCGCTGAAGGCGCTGCTCGTCGACAGCTGGTACG
ACACCTATCTCGGCGTCATGGTTCTCGTGCGCATCATCGACGGCGTGCTGACCAAGGGCCAGACGATCCGCATGATGGGC
ACCGATGCGAAATACCAGGTGGAGCGCGTCGGCGTGCTGACGCCGAAGATGGTCAATGTCGACAGCCTCGGCCCCGGCGA
GATCGGTTTCATCACCGCCTCGATCAAGGAAGTGGCCGACACCCGCGTCGGCGATACGATCACCGAGGACAAGCGGCCGA
CCGCACAAGCGCTGCCGGGCTTCAAGCCGGCGCAGCCGGTGGTGTTCTGCGGCCTGTTCCCGGTCGATGCCGCCGACTTC
GAGGATCTGCGCGCCGCCATGGGCAAGCTTCGCCTCAACGACGCCTCCTTCTCCTTCGAAATGGAATCCTCGGCCGCCCT
CGGCTTCGGCTTCCGCTGCGGCTTCCTCGGCTTGCTGCATCTCGAAATCATCCAGGAACGGCTGGAGCGCGAGTTCGACC
TCGATCTCATCGCCACCGCCCCCTCGGTGGTCTACAAGATGTTCATGACCGACGGCTCGGAGCGCGAGCTGCACAACCCG
GCCGACATGCCCGATGTCGTCAAGATCTCCGAAATCCACGAGCCGTGGATCCGCGCGACGATCCTGACACCCGACGATTA
TCTCGGTGGCATCCTGAAACTCTGCCAGGACCGCCGCGGCATCCAGATCGAGCTCACCTATGTCGGCACGCGCGCCATGC
TGACCTACGACCTGCCGCTCAACGAAGTCGTCTTCGATTTCTACGACCGGCTGAAATCGATTTCCAAGGGCTACGCCTCC
TTCGACTATACGCTGACCGACCACCGCGAGGGCAACCTCGTGAAGATGTCGATCCTGGTCAATGGCGAGCCGGTCGACGC
GCTGTCGATGATGGTGCACCGCACCGCGGCGGAAAAGCGCGGCCGCGACATGTGCGAGAAGCTCAAGGAGCTGATCCCGA
AGCACATGTTCAAGATCCCGATCCAGGCGGCGATCGGCGGCAACGTCATCGCCCGCGAGACGATCTCGGCGCTTCGCAAG
GACGTGACGGCGAAATGCTACGGCGGCGACGCCACCCGCAAGCGCAAGCTGCTCGACAAGCAGAAGGCCGGCAAGAAGCG
CATGCGCCAGTTCGGCAAGGTGGAGATTCCGCAGGAAGCCTTCATCGCAGCGCTGAAGATGGGCGACGAATAA

Upstream 100 bases:

>100_bases
GGCTGGAGACCTGGCCGGTCTGAGACGCGTCCGACCGGCTGACAAAATAGCGTAAACAGCGGGATAATTACCGCTTTGCG
GCAGGGGGCGGCGCTGCTAT

Downstream 100 bases:

>100_bases
CGCTTCTCAATGGCATCTCATGCGCATATGATCTGCGCATGAGACCAAGGAGATCGTTATGGCTCAGCGGCTAAGAAAAG
CATCGAACCTCTCTCTCGAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MARMSTNSTTPLSHIRNFSIVAHIDHGKSTLADRLIQTTGGLAEREMSEQVLDNMDIERERGITIKAQTVRLHYQANNGE
KYILNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEE
VIGIDASEAVLISAKTGLGIPDVLEAIVNRLPAPKSPGGDKAPLKALLVDSWYDTYLGVMVLVRIIDGVLTKGQTIRMMG
TDAKYQVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADF
EDLRAAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKMFMTDGSERELHNP
ADMPDVVKISEIHEPWIRATILTPDDYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYAS
FDYTLTDHREGNLVKMSILVNGEPVDALSMMVHRTAAEKRGRDMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALRK
DVTAKCYGGDATRKRKLLDKQKAGKKRMRQFGKVEIPQEAFIAALKMGDE

Sequences:

>Translated_610_residues
MARMSTNSTTPLSHIRNFSIVAHIDHGKSTLADRLIQTTGGLAEREMSEQVLDNMDIERERGITIKAQTVRLHYQANNGE
KYILNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEE
VIGIDASEAVLISAKTGLGIPDVLEAIVNRLPAPKSPGGDKAPLKALLVDSWYDTYLGVMVLVRIIDGVLTKGQTIRMMG
TDAKYQVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADF
EDLRAAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKMFMTDGSERELHNP
ADMPDVVKISEIHEPWIRATILTPDDYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYAS
FDYTLTDHREGNLVKMSILVNGEPVDALSMMVHRTAAEKRGRDMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALRK
DVTAKCYGGDATRKRKLLDKQKAGKKRMRQFGKVEIPQEAFIAALKMGDE
>Mature_609_residues
ARMSTNSTTPLSHIRNFSIVAHIDHGKSTLADRLIQTTGGLAEREMSEQVLDNMDIERERGITIKAQTVRLHYQANNGEK
YILNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEEV
IGIDASEAVLISAKTGLGIPDVLEAIVNRLPAPKSPGGDKAPLKALLVDSWYDTYLGVMVLVRIIDGVLTKGQTIRMMGT
DAKYQVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAQALPGFKPAQPVVFCGLFPVDAADFE
DLRAAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKMFMTDGSERELHNPA
DMPDVVKISEIHEPWIRATILTPDDYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYASF
DYTLTDHREGNLVKMSILVNGEPVDALSMMVHRTAAEKRGRDMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALRKD
VTAKCYGGDATRKRKLLDKQKAGKKRMRQFGKVEIPQEAFIAALKMGDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=49.3421052631579, Blast_Score=613, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=47.0149253731343, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI18390331, Length=172, Percent_Identity=36.046511627907, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40.6896551724138, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI25306283, Length=182, Percent_Identity=40.1098901098901, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=182, Percent_Identity=40.1098901098901, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=182, Percent_Identity=40.1098901098901, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=35.8208955223881, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI94966752, Length=94, Percent_Identity=44.6808510638298, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI53729339, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=241, Percent_Identity=27.8008298755187, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=56.7612687813022, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=26.5748031496063, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1789738, Length=162, Percent_Identity=36.4197530864198, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790835, Length=158, Percent_Identity=32.2784810126582, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789559, Length=225, Percent_Identity=29.7777777777778, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=38.562091503268, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71988811, Length=139, Percent_Identity=38.8489208633094, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988819, Length=139, Percent_Identity=38.8489208633094, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556745, Length=162, Percent_Identity=35.8024691358025, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.1794871794872, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=37.4149659863946, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=221, Percent_Identity=26.2443438914027, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=250, Percent_Identity=26.8, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=605, Percent_Identity=44.297520661157, Blast_Score=535, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=39.7260273972603, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=39.7260273972603, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=188, Percent_Identity=36.1702127659575, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=125, Percent_Identity=36.8, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=172, Percent_Identity=35.4651162790698, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=44.9586776859504, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=44.9275362318841, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=36.8098159509202, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.8378378378378, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.8378378378378, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.8378378378378, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357743, Length=168, Percent_Identity=35.1190476190476, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI221458488, Length=141, Percent_Identity=40.4255319148936, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67397; Mature: 67265

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMSTNSTTPLSHIRNFSIVAHIDHGKSTLADRLIQTTGGLAEREMSEQVLDNMDIERE
CCCCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
RGITIKAQTVRLHYQANNGEKYILNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVE
CCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCC
AQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEEVIGIDASEAVLISAKTGLGI
HHHHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PDVLEAIVNRLPAPKSPGGDKAPLKALLVDSWYDTYLGVMVLVRIIDGVLTKGQTIRMMG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
TDAKYQVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAQALPG
CCCCEEEEEECCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHCCC
FKPAQPVVFCGLFPVDAADFEDLRAAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLH
CCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHH
LEIIQERLEREFDLDLIATAPSVVYKMFMTDGSERELHNPADMPDVVKISEIHEPWIRAT
HHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHCCCHHEEE
ILTPDDYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYAS
EECCHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHCCCCE
FDYTLTDHREGNLVKMSILVNGEPVDALSMMVHRTAAEKRGRDMCEKLKELIPKHMFKIP
EEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IQAAIGGNVIARETISALRKDVTAKCYGGDATRKRKLLDKQKAGKKRMRQFGKVEIPQEA
HHHHCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FIAALKMGDE
HHHHHHCCCC
>Mature Secondary Structure 
ARMSTNSTTPLSHIRNFSIVAHIDHGKSTLADRLIQTTGGLAEREMSEQVLDNMDIERE
CCCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
RGITIKAQTVRLHYQANNGEKYILNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVE
CCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCC
AQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEEVIGIDASEAVLISAKTGLGI
HHHHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
PDVLEAIVNRLPAPKSPGGDKAPLKALLVDSWYDTYLGVMVLVRIIDGVLTKGQTIRMMG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
TDAKYQVERVGVLTPKMVNVDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAQALPG
CCCCEEEEEECCCCCCEECCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHCCC
FKPAQPVVFCGLFPVDAADFEDLRAAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLH
CCCCCCEEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHH
LEIIQERLEREFDLDLIATAPSVVYKMFMTDGSERELHNPADMPDVVKISEIHEPWIRAT
HHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHCCCHHEEE
ILTPDDYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYAS
EECCHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHCCCCE
FDYTLTDHREGNLVKMSILVNGEPVDALSMMVHRTAAEKRGRDMCEKLKELIPKHMFKIP
EEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IQAAIGGNVIARETISALRKDVTAKCYGGDATRKRKLLDKQKAGKKRMRQFGKVEIPQEA
HHHHCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
FIAALKMGDE
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA