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The map label for this gene is tenA [H]

Identifier: 209551824

GI number: 209551824

Start: 4429791

End: 4430489

Strand: Reverse

Name: tenA [H]

Synonym: Rleg2_4256

Alternate gene names: 209551824

Gene position: 4430489-4429791 (Counterclockwise)

Preceding gene: 209551827

Following gene: 209551823

Centisome position: 97.63

GC content: 62.66

Gene sequence:

>699_bases
ATGGACGCCGGCACGCAAAGCACGGAACTGACGGGAAGCTTCACGGCCGCCGCCTGGGACAGGATTGCGCCCGTCATGGC
CGAGATCGAGGTGCTGCCACTTCTCACGCGTCTTTCCGACGGCACGCTGCCGCCTCAGGTCTTCCGCCACTACATCCTCC
AGGATGCGCTTTACCTCAAGCATTATGCCCGCTGCCTGGCGATCGTCGCAGCCAAGGCTCCTGACAATGCGCAGGTGCTG
CGTTTCCTCGGTTCGGCGCAGAAGGCGATCACCGTCGAGCAGGGCCTGCATGCCGGCTTCCTCACCCAGTTCGGCATCAG
CTCCGAGGATGTCACCGCAGCCGAACCCTCGCCCTCCTGTTTCGCCTATACGAATTTCCTGCTCGCCACCGCCTATCACA
GCTCCTATGCAGTCGCACTTTCTTCAATCCTTCCCTGCTTCTGGATCTACTGGCATGTCGGCGAGGCGATCAAGAGCCGG
CCGGCGATCGAGGGCAACGCCTTCCAGGCCTGGATCAACACCTATGGCGATCCGCAATTTGCCGCCGGCGCCCGCGAAGT
GATCGCCCTGACCGATATCGCCGCCCGCGCCGCATCGCCTTCGGAGCGTGCTCAGATGATGGATGTTTTCGTGCGCGCCT
CGCAATATGAATGGATGTTCTGGGATTCGGCCTGGCGGCTGGAGACCTGGCCGGTCTGA

Upstream 100 bases:

>100_bases
TCTGATGATGGAATCAAGCCTCGGCAAGGCGGGCAAACCAACCTTGCTTTCGCCACAGCCTCGCCGATATATGACAGCGC
AATTGAGATCGGAGAGAGTG

Downstream 100 bases:

>100_bases
GACGCGTCCGACCGGCTGACAAAATAGCGTAAACAGCGGGATAATTACCGCTTTGCGGCAGGGGGCGGCGCTGCTATATG
GCGCGCATGAGCACCAATTC

Product: TenA family transcriptional regulator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDAGTQSTELTGSFTAAAWDRIAPVMAEIEVLPLLTRLSDGTLPPQVFRHYILQDALYLKHYARCLAIVAAKAPDNAQVL
RFLGSAQKAITVEQGLHAGFLTQFGISSEDVTAAEPSPSCFAYTNFLLATAYHSSYAVALSSILPCFWIYWHVGEAIKSR
PAIEGNAFQAWINTYGDPQFAAGAREVIALTDIAARAASPSERAQMMDVFVRASQYEWMFWDSAWRLETWPV

Sequences:

>Translated_232_residues
MDAGTQSTELTGSFTAAAWDRIAPVMAEIEVLPLLTRLSDGTLPPQVFRHYILQDALYLKHYARCLAIVAAKAPDNAQVL
RFLGSAQKAITVEQGLHAGFLTQFGISSEDVTAAEPSPSCFAYTNFLLATAYHSSYAVALSSILPCFWIYWHVGEAIKSR
PAIEGNAFQAWINTYGDPQFAAGAREVIALTDIAARAASPSERAQMMDVFVRASQYEWMFWDSAWRLETWPV
>Mature_232_residues
MDAGTQSTELTGSFTAAAWDRIAPVMAEIEVLPLLTRLSDGTLPPQVFRHYILQDALYLKHYARCLAIVAAKAPDNAQVL
RFLGSAQKAITVEQGLHAGFLTQFGISSEDVTAAEPSPSCFAYTNFLLATAYHSSYAVALSSILPCFWIYWHVGEAIKSR
PAIEGNAFQAWINTYGDPQFAAGAREVIALTDIAARAASPSERAQMMDVFVRASQYEWMFWDSAWRLETWPV

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=201, Percent_Identity=24.3781094527363, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=201, Percent_Identity=23.3830845771144, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325378, Length=196, Percent_Identity=22.9591836734694, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25603; Mature: 25603

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAGTQSTELTGSFTAAAWDRIAPVMAEIEVLPLLTRLSDGTLPPQVFRHYILQDALYLK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HYARCLAIVAAKAPDNAQVLRFLGSAQKAITVEQGLHAGFLTQFGISSEDVTAAEPSPSC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
FAYTNFLLATAYHSSYAVALSSILPCFWIYWHVGEAIKSRPAIEGNAFQAWINTYGDPQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
AAGAREVIALTDIAARAASPSERAQMMDVFVRASQYEWMFWDSAWRLETWPV
HCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCC
>Mature Secondary Structure
MDAGTQSTELTGSFTAAAWDRIAPVMAEIEVLPLLTRLSDGTLPPQVFRHYILQDALYLK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HYARCLAIVAAKAPDNAQVLRFLGSAQKAITVEQGLHAGFLTQFGISSEDVTAAEPSPSC
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEHHHCCCHHHHHHCCCCCCCCCCCCCCCCH
FAYTNFLLATAYHSSYAVALSSILPCFWIYWHVGEAIKSRPAIEGNAFQAWINTYGDPQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCH
AAGAREVIALTDIAARAASPSERAQMMDVFVRASQYEWMFWDSAWRLETWPV
HCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]