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The map label for this gene is gcvA [H]

Identifier: 209551680

GI number: 209551680

Start: 4291038

End: 4292009

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_4109

Alternate gene names: 209551680

Gene position: 4291038-4292009 (Clockwise)

Preceding gene: 209551675

Following gene: 209551696

Centisome position: 94.56

GC content: 61.11

Gene sequence:

>972_bases
TTGCGCAGAGGGAAATCATCGCCAGATGAACAGGAAGGCGTTCCGAAACGCGGACGTTTACCGCCGCTTTCGATGGTGCG
CGCCTTCGAGGCGGTCAGCCGTTTGGGCTCGATGCGTAGAGCTGCGGACGATCTCAATCTCAGTCACACCGTCATATCCC
GCCACGTCCGCAATCTCGAAGCCTGGTTTGGCACGAGGCTTGTCGATGCCGGACCGCGCGGCATCAGGCTGACGGCGGAA
GGCAGGGTTTTTGCCGAGACGGTAGGGGCGGCTTTCGACCTCATCGCCAAATCGACGGCCGAACTGCGTCCGGCAGTCAA
TCGCACGGTCTTGCGCATCTGGTGCGTGCCCGGTTTCGCGACACGGTGGCTGACGCCGCGCTTCAACCAACTCCAGCTTG
CCTTGCCGGGCATCGATTTCGTGATGCGCGCGACCGATCTCGAACCGGATTTCACCCGTTATGAGGCCGATATCGACATC
CGCTATTCCGAGGAGCCGAGGGGGAATGTCCGCTGGATCCTCTTGGAAAGGCCGCGCATGTTCCCGGTCACCAGCCCCGA
ATGGATCAGCAGAAACGCTCCGATACGAACCCTCGATGATCTGGTACGACAGCCGCTGATCCACGAGGAAAGCCGCGATC
AGTGGCGGCAATGGTTTCGCAAGGCTGGCTTCGAGCATGAAGTGACATTGAACGGTCCGCGACTTTGGTCCGCCAATCTG
ACGGTCGACGCGGCAGTGGCAGGCCAAGGAATAGCGCTGGCCACCCGCCTGATCGCCGCGGACGACTTGCGTGCCGGGCG
GCTCGTCGAACTGCTTGAAACCGATGTCCGGCTCGGCGGATATTATTTAGTCGGGCCACGTGAGCGCTGGAATGAGGTAA
CATCGGCGCGTTTCCGAACATGGCTGCAGCAGTCGATCGCTGAAACCACGGAAGGACACGCCCCCAACGAAGGCGGAAAT
CCAAGCCGATAG

Upstream 100 bases:

>100_bases
CTCTTCGTTACGCCGAAATCGCCAATTCCATGGCATATGAAGGGAACATGGCATTCCGATCTTGGTTCGGCGGAACCAAT
TTCCGTTCTGGGAGACAGGA

Downstream 100 bases:

>100_bases
GCGATATCCGTGAGCGTTTGACAAGGAGACGAGCTTCAACATCACGCGCGATCGGCGGGCGGAGGGTGCTACGCACTCCG
ATTCAGCTCCTCTGCCAATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRRGKSSPDEQEGVPKRGRLPPLSMVRAFEAVSRLGSMRRAADDLNLSHTVISRHVRNLEAWFGTRLVDAGPRGIRLTAE
GRVFAETVGAAFDLIAKSTAELRPAVNRTVLRIWCVPGFATRWLTPRFNQLQLALPGIDFVMRATDLEPDFTRYEADIDI
RYSEEPRGNVRWILLERPRMFPVTSPEWISRNAPIRTLDDLVRQPLIHEESRDQWRQWFRKAGFEHEVTLNGPRLWSANL
TVDAAVAGQGIALATRLIAADDLRAGRLVELLETDVRLGGYYLVGPRERWNEVTSARFRTWLQQSIAETTEGHAPNEGGN
PSR

Sequences:

>Translated_323_residues
MRRGKSSPDEQEGVPKRGRLPPLSMVRAFEAVSRLGSMRRAADDLNLSHTVISRHVRNLEAWFGTRLVDAGPRGIRLTAE
GRVFAETVGAAFDLIAKSTAELRPAVNRTVLRIWCVPGFATRWLTPRFNQLQLALPGIDFVMRATDLEPDFTRYEADIDI
RYSEEPRGNVRWILLERPRMFPVTSPEWISRNAPIRTLDDLVRQPLIHEESRDQWRQWFRKAGFEHEVTLNGPRLWSANL
TVDAAVAGQGIALATRLIAADDLRAGRLVELLETDVRLGGYYLVGPRERWNEVTSARFRTWLQQSIAETTEGHAPNEGGN
PSR
>Mature_323_residues
MRRGKSSPDEQEGVPKRGRLPPLSMVRAFEAVSRLGSMRRAADDLNLSHTVISRHVRNLEAWFGTRLVDAGPRGIRLTAE
GRVFAETVGAAFDLIAKSTAELRPAVNRTVLRIWCVPGFATRWLTPRFNQLQLALPGIDFVMRATDLEPDFTRYEADIDI
RYSEEPRGNVRWILLERPRMFPVTSPEWISRNAPIRTLDDLVRQPLIHEESRDQWRQWFRKAGFEHEVTLNGPRLWSANL
TVDAAVAGQGIALATRLIAADDLRAGRLVELLETDVRLGGYYLVGPRERWNEVTSARFRTWLQQSIAETTEGHAPNEGGN
PSR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=31.3131313131313, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=31.2280701754386, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=23.1543624161074, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI145693193, Length=261, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36653; Mature: 36653

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGKSSPDEQEGVPKRGRLPPLSMVRAFEAVSRLGSMRRAADDLNLSHTVISRHVRNLE
CCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AWFGTRLVDAGPRGIRLTAEGRVFAETVGAAFDLIAKSTAELRPAVNRTVLRIWCVPGFA
HHHCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHH
TRWLTPRFNQLQLALPGIDFVMRATDLEPDFTRYEADIDIRYSEEPRGNVRWILLERPRM
HHCCCCCCCEEEEECCCHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCCEEEEEEECCCC
FPVTSPEWISRNAPIRTLDDLVRQPLIHEESRDQWRQWFRKAGFEHEVTLNGPRLWSANL
CCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCC
TVDAAVAGQGIALATRLIAADDLRAGRLVELLETDVRLGGYYLVGPRERWNEVTSARFRT
EEEEHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
WLQQSIAETTEGHAPNEGGNPSR
HHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRRGKSSPDEQEGVPKRGRLPPLSMVRAFEAVSRLGSMRRAADDLNLSHTVISRHVRNLE
CCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AWFGTRLVDAGPRGIRLTAEGRVFAETVGAAFDLIAKSTAELRPAVNRTVLRIWCVPGFA
HHHCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHH
TRWLTPRFNQLQLALPGIDFVMRATDLEPDFTRYEADIDIRYSEEPRGNVRWILLERPRM
HHCCCCCCCEEEEECCCHHHHHHHCCCCCCHHEEEEEEEEEECCCCCCCEEEEEEECCCC
FPVTSPEWISRNAPIRTLDDLVRQPLIHEESRDQWRQWFRKAGFEHEVTLNGPRLWSANL
CCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEEEECCC
TVDAAVAGQGIALATRLIAADDLRAGRLVELLETDVRLGGYYLVGPRERWNEVTSARFRT
EEEEHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
WLQQSIAETTEGHAPNEGGNPSR
HHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]