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The map label for this gene is phnF [C]

Identifier: 209551696

GI number: 209551696

Start: 4304881

End: 4305618

Strand: Direct

Name: phnF [C]

Synonym: Rleg2_4125

Alternate gene names: 209551696

Gene position: 4304881-4305618 (Clockwise)

Preceding gene: 209551680

Following gene: 209551703

Centisome position: 94.86

GC content: 64.09

Gene sequence:

>738_bases
ATGGCAGGATTGAAACAGGTGCAAAGGCAGACCGGCGTGGCGCTCTGGCGTCAGATCGCTGACCGGATTCGCGAGGCGAT
CGGCAACGGTACCTATGACGAAACCGGAATGGTGCCGCCGGAAACGGTCCTGGCGCTGCAATTCGGCGTCAACCGACATA
CGGTGCGCAGCGCCCTGGCGGCGCTGGCGCAGGAAGGCATCGTCCGTGCGGTACAAGGTCGCGGCACGCTGATCGAGCGT
AAGGAGCGGCTGAATTTCCCGATCACCCGGCGCACCCGCTTCACTTCAGGCATCGGCGACCAGGCGCGCGAGATGCGCAG
CCTGCTGCTCGATGAGGTGCAGGAGGAAGCCAGCGCCGAGGTGGCCCGCTGGCTGGGTCTGAAGCAAGGGGAGCAGGTCA
TCCGGCTGGAAACATTGCGCCAGGCCGACAAACGGCCAGTTTCAAAGGCGACAAGCTGGTTTCCCGCCGCACGCTTCGCC
GGGATCGGCGAGGCCTATCGCAAGCGCGAGTCGATCACCAAGGCCTTCGCCGATCTTGGCCTGTCGGATTATGTCCGTGC
GACGACCGAAGTGACGGCCGCCCATGCGAACACGGCGGATCTCGCCGATCTGGAACTGACCCCCGGCGCGATCCTTCTGA
TCGCCAAGGCGATGAACACCGATCTCGACGGTGTGCCGGTGCAATACTCGATCAGCCGGTTTGCGGCCGACCGGGTGCAG
TTCACCATCGAGAACTAA

Upstream 100 bases:

>100_bases
AAAATGTCTATTGATATAGACAACCATACAAGCTATGTTTAGTCCTAAATCGATGTCGTGACAAGCGTGTGACATCGGGC
GCGGGAAATGGCGGGAACGA

Downstream 100 bases:

>100_bases
AGCATGTCGCGCAAAAGTGTGCAGCGGTTTTGCCAGGCAAAGCGCGAAGCGCTTTTGCCGCAACGACATGCGTAAAACAA
AGAGCTAAAGCGCGTCGCAT

Product: phophonate C-P lyase system transcriptional regulator PhnF, GntR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAGLKQVQRQTGVALWRQIADRIREAIGNGTYDETGMVPPETVLALQFGVNRHTVRSALAALAQEGIVRAVQGRGTLIER
KERLNFPITRRTRFTSGIGDQAREMRSLLLDEVQEEASAEVARWLGLKQGEQVIRLETLRQADKRPVSKATSWFPAARFA
GIGEAYRKRESITKAFADLGLSDYVRATTEVTAAHANTADLADLELTPGAILLIAKAMNTDLDGVPVQYSISRFAADRVQ
FTIEN

Sequences:

>Translated_245_residues
MAGLKQVQRQTGVALWRQIADRIREAIGNGTYDETGMVPPETVLALQFGVNRHTVRSALAALAQEGIVRAVQGRGTLIER
KERLNFPITRRTRFTSGIGDQAREMRSLLLDEVQEEASAEVARWLGLKQGEQVIRLETLRQADKRPVSKATSWFPAARFA
GIGEAYRKRESITKAFADLGLSDYVRATTEVTAAHANTADLADLELTPGAILLIAKAMNTDLDGVPVQYSISRFAADRVQ
FTIEN
>Mature_244_residues
AGLKQVQRQTGVALWRQIADRIREAIGNGTYDETGMVPPETVLALQFGVNRHTVRSALAALAQEGIVRAVQGRGTLIERK
ERLNFPITRRTRFTSGIGDQAREMRSLLLDEVQEEASAEVARWLGLKQGEQVIRLETLRQADKRPVSKATSWFPAARFAG
IGEAYRKRESITKAFADLGLSDYVRATTEVTAAHANTADLADLELTPGAILLIAKAMNTDLDGVPVQYSISRFAADRVQF
TIEN

Specific function: Belongs To An Operon Involved In Alkylphosphonate Uptake And C-P Lyase. Exact Function Not Known. By Similarity Could Be A Transcriptional Regulator. [C]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790540, Length=238, Percent_Identity=28.9915966386555, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786950, Length=237, Percent_Identity=27.8481012658228, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788418, Length=233, Percent_Identity=24.8927038626609, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082252, Length=243, Percent_Identity=24.6913580246914, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012702
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 27036; Mature: 26905

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLKQVQRQTGVALWRQIADRIREAIGNGTYDETGMVPPETVLALQFGVNRHTVRSALA
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ALAQEGIVRAVQGRGTLIERKERLNFPITRRTRFTSGIGDQAREMRSLLLDEVQEEASAE
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VARWLGLKQGEQVIRLETLRQADKRPVSKATSWFPAARFAGIGEAYRKRESITKAFADLG
HHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
LSDYVRATTEVTAAHANTADLADLELTPGAILLIAKAMNTDLDGVPVQYSISRFAADRVQ
HHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHE
FTIEN
EEECC
>Mature Secondary Structure 
AGLKQVQRQTGVALWRQIADRIREAIGNGTYDETGMVPPETVLALQFGVNRHTVRSALA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
ALAQEGIVRAVQGRGTLIERKERLNFPITRRTRFTSGIGDQAREMRSLLLDEVQEEASAE
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VARWLGLKQGEQVIRLETLRQADKRPVSKATSWFPAARFAGIGEAYRKRESITKAFADLG
HHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
LSDYVRATTEVTAAHANTADLADLELTPGAILLIAKAMNTDLDGVPVQYSISRFAADRVQ
HHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHE
FTIEN
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431; 9889346 [H]