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The map label for this gene is potH [H]

Identifier: 209551677

GI number: 209551677

Start: 4287172

End: 4288341

Strand: Reverse

Name: potH [H]

Synonym: Rleg2_4106

Alternate gene names: 209551677

Gene position: 4288341-4287172 (Counterclockwise)

Preceding gene: 209551678

Following gene: 209551676

Centisome position: 94.5

GC content: 62.91

Gene sequence:

>1170_bases
ATGAATGCTTTCCGTCGACACACCGGCACCTTTGCCCTCGTCTTTCCGTTGACCCTGTTCCTGGGCGTCTTCTTCATCTG
GCCGCTTCTGACCGTCCTGCAACAGGCGGTGTCCGATCCGGTCGTCAGCAAAAACCTGCCGCGGACGACAGCTGCGGTCT
CGGCCTGGAACATTACCTCCGAACCCTCGGACGAAATGCGGACAGCTCTTGTCGACGACCTGAAATCGCTCGACGACGAC
CAGAAACTCGGAGAGGTGGTTCGAAGACTGAACAGCGCCCAGCCGGGCTTCCGCACATTGATGCGAAAGACCGCCGCGGC
CGTGAGAGACAGCACCGGGCCGATAAGCCTCGCAGAGATCGACGCCCGATGGAACGACGTTGCCTTCTGGCGGGCAATCG
CCGAAGCCACCGAGCCTTACACCGATCGCAACCTGCTTGCCGCAGTCGATCTTCAGCATGACGCTTCCGGGGCTATCGTG
GCGATGCCGGCTGGAGAGTCCGCCAATCAGGCGATTCTGCTGAGAACATTCGCCGTGGCCGCCGTCGTGACCCTGGCGAC
CTGTCTCATCGGCCTGCCCTACGCGATGCTGGCCGCCGCCTTGAGCGGCTGGCGGCGGCAGCTCCTCCTTGCCGCGGTAC
TGCTGCCGCTGTGGACATCGCTGCTGGTGCGCACCGCGGCCTGGTACATCCTCCTTCAGGACAAGGGGCTCATCAACTCC
CTCCTCGTGTCGCTCGGCCTTCTCGACAGTCCCTTGCCGCTTCTTTTCAACCGGACGGGTGTCGTCATCGCCATGACGCA
TGTGTTGCTGCCGTTCATGGTTCTGCCGATCTACAGCGTCCTCATCGCAATCCCGCGAAATCTCATGCCAGCGGCAGCCT
CTCTTGGCGCGAGGCCGATCCGGGCCTTTCTGCATATCCTGCTGCCCCTGGCCCTGCGCGGCATCGCCTCCGGCGGACTG
CTCGTCTTCATGGCAGCGCTCGGTTACTACATCACACCGGCGCTCATCGGCGGGCCGAAGAATCAGATGATCAGTTCGGT
GATTGCCTTCTACGCCACCGGCTCGGCCAATTGGGGCATGGCCGGTGCGCTCGGCATGGTGCTGCTCGCAGTGACGATCG
TGCTCTACGGCGTTTATGGCCGGCTATCCATGAAGACGACGGGGGCGTGA

Upstream 100 bases:

>100_bases
TCCGCTTCGTGGTCCGGACCGACATGCGGTCGGCGGAGTGGCCGGTCGGAGCCGATGTGGTGGCGCACTTCACGCCTGAA
GACTGCTGGGTGATTGCAGC

Downstream 100 bases:

>100_bases
CATGACAATTCGATTGCTAAAATTTCTGTTCGGCGCGGCAACGGTCATTTTTCTGCTGGCTCCGCTTTTGGCGATCCTTC
CGATCGCATTTACCTCCAGC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNAFRRHTGTFALVFPLTLFLGVFFIWPLLTVLQQAVSDPVVSKNLPRTTAAVSAWNITSEPSDEMRTALVDDLKSLDDD
QKLGEVVRRLNSAQPGFRTLMRKTAAAVRDSTGPISLAEIDARWNDVAFWRAIAEATEPYTDRNLLAAVDLQHDASGAIV
AMPAGESANQAILLRTFAVAAVVTLATCLIGLPYAMLAAALSGWRRQLLLAAVLLPLWTSLLVRTAAWYILLQDKGLINS
LLVSLGLLDSPLPLLFNRTGVVIAMTHVLLPFMVLPIYSVLIAIPRNLMPAAASLGARPIRAFLHILLPLALRGIASGGL
LVFMAALGYYITPALIGGPKNQMISSVIAFYATGSANWGMAGALGMVLLAVTIVLYGVYGRLSMKTTGA

Sequences:

>Translated_389_residues
MNAFRRHTGTFALVFPLTLFLGVFFIWPLLTVLQQAVSDPVVSKNLPRTTAAVSAWNITSEPSDEMRTALVDDLKSLDDD
QKLGEVVRRLNSAQPGFRTLMRKTAAAVRDSTGPISLAEIDARWNDVAFWRAIAEATEPYTDRNLLAAVDLQHDASGAIV
AMPAGESANQAILLRTFAVAAVVTLATCLIGLPYAMLAAALSGWRRQLLLAAVLLPLWTSLLVRTAAWYILLQDKGLINS
LLVSLGLLDSPLPLLFNRTGVVIAMTHVLLPFMVLPIYSVLIAIPRNLMPAAASLGARPIRAFLHILLPLALRGIASGGL
LVFMAALGYYITPALIGGPKNQMISSVIAFYATGSANWGMAGALGMVLLAVTIVLYGVYGRLSMKTTGA
>Mature_389_residues
MNAFRRHTGTFALVFPLTLFLGVFFIWPLLTVLQQAVSDPVVSKNLPRTTAAVSAWNITSEPSDEMRTALVDDLKSLDDD
QKLGEVVRRLNSAQPGFRTLMRKTAAAVRDSTGPISLAEIDARWNDVAFWRAIAEATEPYTDRNLLAAVDLQHDASGAIV
AMPAGESANQAILLRTFAVAAVVTLATCLIGLPYAMLAAALSGWRRQLLLAAVLLPLWTSLLVRTAAWYILLQDKGLINS
LLVSLGLLDSPLPLLFNRTGVVIAMTHVLLPFMVLPIYSVLIAIPRNLMPAAASLGARPIRAFLHILLPLALRGIASGGL
LVFMAALGYYITPALIGGPKNQMISSVIAFYATGSANWGMAGALGMVLLAVTIVLYGVYGRLSMKTTGA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=38.1395348837209, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787369, Length=212, Percent_Identity=35.8490566037736, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787713, Length=224, Percent_Identity=33.4821428571429, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 41719; Mature: 41719

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFRRHTGTFALVFPLTLFLGVFFIWPLLTVLQQAVSDPVVSKNLPRTTAAVSAWNITS
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHEECCCCC
EPSDEMRTALVDDLKSLDDDQKLGEVVRRLNSAQPGFRTLMRKTAAAVRDSTGPISLAEI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEHHHH
DARWNDVAFWRAIAEATEPYTDRNLLAAVDLQHDASGAIVAMPAGESANQAILLRTFAVA
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
AVVTLATCLIGLPYAMLAAALSGWRRQLLLAAVLLPLWTSLLVRTAAWYILLQDKGLINS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
LLVSLGLLDSPLPLLFNRTGVVIAMTHVLLPFMVLPIYSVLIAIPRNLMPAAASLGARPI
HHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
RAFLHILLPLALRGIASGGLLVFMAALGYYITPALIGGPKNQMISSVIAFYATGSANWGM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCH
AGALGMVLLAVTIVLYGVYGRLSMKTTGA
HHHHHHHHHHHHHHHHHHHHCEEECCCCC
>Mature Secondary Structure
MNAFRRHTGTFALVFPLTLFLGVFFIWPLLTVLQQAVSDPVVSKNLPRTTAAVSAWNITS
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHEECCCCC
EPSDEMRTALVDDLKSLDDDQKLGEVVRRLNSAQPGFRTLMRKTAAAVRDSTGPISLAEI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEHHHH
DARWNDVAFWRAIAEATEPYTDRNLLAAVDLQHDASGAIVAMPAGESANQAILLRTFAVA
CCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHH
AVVTLATCLIGLPYAMLAAALSGWRRQLLLAAVLLPLWTSLLVRTAAWYILLQDKGLINS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHH
LLVSLGLLDSPLPLLFNRTGVVIAMTHVLLPFMVLPIYSVLIAIPRNLMPAAASLGARPI
HHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
RAFLHILLPLALRGIASGGLLVFMAALGYYITPALIGGPKNQMISSVIAFYATGSANWGM
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCH
AGALGMVLLAVTIVLYGVYGRLSMKTTGA
HHHHHHHHHHHHHHHHHHHHCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]