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The map label for this gene is potI [H]

Identifier: 209551676

GI number: 209551676

Start: 4286382

End: 4287170

Strand: Reverse

Name: potI [H]

Synonym: Rleg2_4105

Alternate gene names: 209551676

Gene position: 4287170-4286382 (Counterclockwise)

Preceding gene: 209551677

Following gene: 209551673

Centisome position: 94.47

GC content: 61.34

Gene sequence:

>789_bases
ATGACAATTCGATTGCTAAAATTTCTGTTCGGCGCGGCAACGGTCATTTTTCTGCTGGCTCCGCTTTTGGCGATCCTTCC
GATCGCATTTACCTCCAGCAGCTTCCTGGCCTATCCCATTCCTTCTTTTTCGGATCGCTGGTTTGCGGAACTGGCAACCG
AGGACGCCTGGCGACGGTCTATCGTCAACAGCTTGCTGATCGGAGCGGGGACCACCTTGCTTTCCACCGTGCTCGGCATT
CTGGCCGCACTGGCGTTGCGCAGGCGCGTTGTTTTTGCCGCGGCGCTGAGGACGATATTCCTCCTGCCGATGGTCGTTCC
CGCCGTCGTGCTCGGCGTGGGGCTGCAGATCCTGTTTGCCGGCCTCGGATTTCCAAACACATTTGCGGCCGTCATCGTGG
CACATACGGTTGTCGCCGTGCCTTTTGTGCTGGTCAGCATCACCGCCTCGCTTGAGGGCATCGACTATCGGACCGAGCGT
GCGGCCGCGAGCCTCGGGGCTTCTCCGGCGAAGGTCTTCTGGAAAGTGACGCTGCCGCTCGCCATGCCCGGCGTCCTTTC
CGGCGCGGTGCTTGCCTTTGCGACATCGCTTGACGAAGTCGTGCTGACGCTGTTTGTCGCCGGACCAAACCAGCTGACGC
TGGCGCGCCAGATGTTCTCGTCGATTCGCGAAAACATCAGTCCGGCCATCGTGGCGGCAGCATTTCTGTTTATCGTCGGG
ACGATCTTTCTCGGAGGCCTGCTCGCGGTGGTGCACAGACGCGCCGCCCGGTCTGCGGCCCCGGCCTGA

Upstream 100 bases:

>100_bases
TGGGGCATGGCCGGTGCGCTCGGCATGGTGCTGCTCGCAGTGACGATCGTGCTCTACGGCGTTTATGGCCGGCTATCCAT
GAAGACGACGGGGGCGTGAC

Downstream 100 bases:

>100_bases
GGCCAGGGCCGTTCGTCCTTCGGGGAACCGACGGCTCCCGTTACTCGCCGTCGCCCCGACCGGACACGATCTCGCGCTTG
CCGACATGATTGGCCGGGCC

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTIRLLKFLFGAATVIFLLAPLLAILPIAFTSSSFLAYPIPSFSDRWFAELATEDAWRRSIVNSLLIGAGTTLLSTVLGI
LAALALRRRVVFAAALRTIFLLPMVVPAVVLGVGLQILFAGLGFPNTFAAVIVAHTVVAVPFVLVSITASLEGIDYRTER
AAASLGASPAKVFWKVTLPLAMPGVLSGAVLAFATSLDEVVLTLFVAGPNQLTLARQMFSSIRENISPAIVAAAFLFIVG
TIFLGGLLAVVHRRAARSAAPA

Sequences:

>Translated_262_residues
MTIRLLKFLFGAATVIFLLAPLLAILPIAFTSSSFLAYPIPSFSDRWFAELATEDAWRRSIVNSLLIGAGTTLLSTVLGI
LAALALRRRVVFAAALRTIFLLPMVVPAVVLGVGLQILFAGLGFPNTFAAVIVAHTVVAVPFVLVSITASLEGIDYRTER
AAASLGASPAKVFWKVTLPLAMPGVLSGAVLAFATSLDEVVLTLFVAGPNQLTLARQMFSSIRENISPAIVAAAFLFIVG
TIFLGGLLAVVHRRAARSAAPA
>Mature_261_residues
TIRLLKFLFGAATVIFLLAPLLAILPIAFTSSSFLAYPIPSFSDRWFAELATEDAWRRSIVNSLLIGAGTTLLSTVLGIL
AALALRRRVVFAAALRTIFLLPMVVPAVVLGVGLQILFAGLGFPNTFAAVIVAHTVVAVPFVLVSITASLEGIDYRTERA
AASLGASPAKVFWKVTLPLAMPGVLSGAVLAFATSLDEVVLTLFVAGPNQLTLARQMFSSIRENISPAIVAAAFLFIVGT
IFLGGLLAVVHRRAARSAAPA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=241, Percent_Identity=36.0995850622407, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787368, Length=212, Percent_Identity=32.5471698113208, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787714, Length=199, Percent_Identity=33.1658291457286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786980, Length=79, Percent_Identity=41.7721518987342, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27587; Mature: 27456

Theoretical pI: Translated: 11.77; Mature: 11.77

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRLLKFLFGAATVIFLLAPLLAILPIAFTSSSFLAYPIPSFSDRWFAELATEDAWRRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
IVNSLLIGAGTTLLSTVLGILAALALRRRVVFAAALRTIFLLPMVVPAVVLGVGLQILFA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGFPNTFAAVIVAHTVVAVPFVLVSITASLEGIDYRTERAAASLGASPAKVFWKVTLPL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHEEEEHHH
AMPGVLSGAVLAFATSLDEVVLTLFVAGPNQLTLARQMFSSIRENISPAIVAAAFLFIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TIFLGGLLAVVHRRAARSAAPA
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIRLLKFLFGAATVIFLLAPLLAILPIAFTSSSFLAYPIPSFSDRWFAELATEDAWRRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHH
IVNSLLIGAGTTLLSTVLGILAALALRRRVVFAAALRTIFLLPMVVPAVVLGVGLQILFA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGFPNTFAAVIVAHTVVAVPFVLVSITASLEGIDYRTERAAASLGASPAKVFWKVTLPL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHEEEEHHH
AMPGVLSGAVLAFATSLDEVVLTLFVAGPNQLTLARQMFSSIRENISPAIVAAAFLFIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TIFLGGLLAVVHRRAARSAAPA
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]