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The map label for this gene is cynT [H]

Identifier: 209551482

GI number: 209551482

Start: 4053175

End: 4053816

Strand: Reverse

Name: cynT [H]

Synonym: Rleg2_3911

Alternate gene names: 209551482

Gene position: 4053816-4053175 (Counterclockwise)

Preceding gene: 209551483

Following gene: 209551481

Centisome position: 89.33

GC content: 60.59

Gene sequence:

>642_bases
ATGCAGCGTTTTCCAAATTCCCTTCTGGACGGTTACCGCAACTTCATGAACGGGCGTTATGCCGACGCCCGCGACAGGTA
TCGGCTGCTTGCCGAAAACGGTCAGAGTCCGAGCACGCTGGTCATTGCCTGTTCGGATTCACGTGCGGCACCGGAGCTGA
TCTTCGATGCCGGCCCGGGTGAGCTCTTCGTTATCCGCAACGTCGCCAACATGGTGCCGCCCTACGAGCCGGACGGCCAT
TTCCATTCGACGTCGGCCGCGCTCGAATTTGCGGTGCAGGCGCTGAAGGTCTCGGATATCGTCGTGATGGGCCATGGCCG
CTGCGGCGGCATCCGCGCCGCGCTCGACCCCAATGCCGAGCCGCTGTCGCCGGGCGATTTCATCGGCCGCTGGATGTCGC
TGGTCAAGCCTGCCGCCGAGCAGATCCAGAGCAATGACGTGATGACGGCCGCCGAGCGGCAGACGGCGCTGGAGCGCGTC
TCGATCCGCAATTCGATCGAAAATCTCAGAAGCTTTCCCGATATCAAGGCACTCGAGGAAGCGGGAAAAATGCATCTCCA
TGGCGCATGGTTCGATATTTCCACAGGCGAACTCTGGGTGATGGATGCTGAGACACGTGACTTCATTCGTCCCGAAATCT
AG

Upstream 100 bases:

>100_bases
TGCACCCGGCGCAGCGGCGGAAAAAATGACGCATCCATCAATGTGCTCATTTTGCAGTGCAGCAGTTGATCTTGTCTTCC
GCGCGCGCTAATCCAGAAGC

Downstream 100 bases:

>100_bases
GGATCTGCCGCTTTATCAGTTTATTAAGAATTGCTGCTAAGATTGGCGTCAATTGGTCGCGCACGACCGACGTTTTGAGC
ACCGTGGCGATTGGCGATGA

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQRFPNSLLDGYRNFMNGRYADARDRYRLLAENGQSPSTLVIACSDSRAAPELIFDAGPGELFVIRNVANMVPPYEPDGH
FHSTSAALEFAVQALKVSDIVVMGHGRCGGIRAALDPNAEPLSPGDFIGRWMSLVKPAAEQIQSNDVMTAAERQTALERV
SIRNSIENLRSFPDIKALEEAGKMHLHGAWFDISTGELWVMDAETRDFIRPEI

Sequences:

>Translated_213_residues
MQRFPNSLLDGYRNFMNGRYADARDRYRLLAENGQSPSTLVIACSDSRAAPELIFDAGPGELFVIRNVANMVPPYEPDGH
FHSTSAALEFAVQALKVSDIVVMGHGRCGGIRAALDPNAEPLSPGDFIGRWMSLVKPAAEQIQSNDVMTAAERQTALERV
SIRNSIENLRSFPDIKALEEAGKMHLHGAWFDISTGELWVMDAETRDFIRPEI
>Mature_213_residues
MQRFPNSLLDGYRNFMNGRYADARDRYRLLAENGQSPSTLVIACSDSRAAPELIFDAGPGELFVIRNVANMVPPYEPDGH
FHSTSAALEFAVQALKVSDIVVMGHGRCGGIRAALDPNAEPLSPGDFIGRWMSLVKPAAEQIQSNDVMTAAERQTALERV
SIRNSIENLRSFPDIKALEEAGKMHLHGAWFDISTGELWVMDAETRDFIRPEI

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=204, Percent_Identity=34.8039215686275, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786318, Length=82, Percent_Identity=41.4634146341463, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25147564, Length=236, Percent_Identity=26.6949152542373, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115532990, Length=238, Percent_Identity=25.2100840336134, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324292, Length=177, Percent_Identity=26.5536723163842, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23590; Mature: 23590

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFPNSLLDGYRNFMNGRYADARDRYRLLAENGQSPSTLVIACSDSRAAPELIFDAGPG
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCC
ELFVIRNVANMVPPYEPDGHFHSTSAALEFAVQALKVSDIVVMGHGRCGGIRAALDPNAE
CEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCC
PLSPGDFIGRWMSLVKPAAEQIQSNDVMTAAERQTALERVSIRNSIENLRSFPDIKALEE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AGKMHLHGAWFDISTGELWVMDAETRDFIRPEI
CCCEEEEEEEEEECCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MQRFPNSLLDGYRNFMNGRYADARDRYRLLAENGQSPSTLVIACSDSRAAPELIFDAGPG
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCC
ELFVIRNVANMVPPYEPDGHFHSTSAALEFAVQALKVSDIVVMGHGRCGGIRAALDPNAE
CEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCC
PLSPGDFIGRWMSLVKPAAEQIQSNDVMTAAERQTALERVSIRNSIENLRSFPDIKALEE
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AGKMHLHGAWFDISTGELWVMDAETRDFIRPEI
CCCEEEEEEEEEECCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]