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The map label for this gene is pleD [H]

Identifier: 209551481

GI number: 209551481

Start: 4051465

End: 4053078

Strand: Reverse

Name: pleD [H]

Synonym: Rleg2_3910

Alternate gene names: 209551481

Gene position: 4053078-4051465 (Counterclockwise)

Preceding gene: 209551482

Following gene: 209551477

Centisome position: 89.32

GC content: 62.08

Gene sequence:

>1614_bases
ATGAAGTTCGAGACCATCAAAAGGGTTATCCTGGCCGCCATCATGCTGCCCTTCGTCTTCATGCTCATTGAAGGCGTCGT
CGTCATACGAGCTTCAGTCAACCAATACAGGGATCTCGAGAAAGACCGGCAGTTCGCCGACGTTCTTGCCCGCGGCGGGT
CGATCGCCGCCACGGAGATCCTGAGTGAAATCGGCGCCACTCGCCTTTATCTCGCGCATCCGAGCAGCAAGACTGCCGCT
GATATGCAGAAAAGCCGGGCAACGCTCGATGACGAGCGCCTTGCCTTCTATGCCAGCCTGCCTTCCCGCGATACGCTCGA
TGAAGGGCTTGCGGAGGAATTGTCGATTCTCAGCCTTGCCTACAGCCGTATCGTCGCCGCGCGCAGCGCCGTCGACCAGG
GCCGTTATGTCGGCAGCGATCCCGGGTCGATCTACTGGTATGCGGCTCTCAAGCAGCTTGCCGTCGTCGATGCGCTTTCA
CCCTTGATCAGCGATCCGGTGCTGCTGGAGAAATCCAACCAGCTGATGGGCATCATGCTGACCTATTATGGGGAGAGGCT
GATCACCGGCATTGGCAGCCGTTACCTCGACCAGGGCGTGTCCGCCAGATTTCCGGTGGAGCTGTTCGTCCAGGGCAAGG
TCATGATCGGCGAGGGCATGGATCACATGGTCTTCCATTCGGCGGCGCCGATCGTGCGCGACATTGTCGCCTATCTCGGT
CGCCGCGACCAGGTGAAGGCGAATGCGATCACCGATGCAATCCTCGCCGGATCGCGGCCGACGCCCGCGGTGCGCGACGT
CTGGGCGGCTGCGCAGAGCGAGCGCATGACTTTCCTGCAGCAGAAGATGATCGAGGCGGCGAGAGATATTCACGAAACCG
GCGAAAACTTTTCGACGCGCTCGCATTTACACCTGACGCGGATCCTGGCGCTGTGCGCCGGCCTGCTCATTCTCGCGACA
CTGGTGATGGTGCTGGCGGCAAGGGGTCTGCGCCTGATCGACCGGCTGTCGCGGGATCGGGAGACGCTGGTCGGCGAGTT
GCGCAACGCCGCGCAGACCGATCTCCTGACCGGTCTTTACAACAGGCGCGGCTTCGAGTTCGCCGCATCGGCGCTTCTCA
CGCAGGCCGAGCACGGATCGCGCTGGATTTCCGTCGTGCTCTTCGACCTCGATCATTTCAAGAAGATCAACGATATCAAT
GGCCATGACGCTGGCGATGCCGTGCTCCGGCATGTCGCAGGCGTCGCGCGCCAGAATTTCCGCTCCTTCGATCTCTTGGT
GCGCCATGGCGGCGAGGAGTTCCTGGCGCTCCTGCCGGATTCGACCCCCGACGATGCGGCAATCGTTGCCGAGCGCGTAA
GGCTGGCGATCGAGGCGGCGGAAATCCCCTTGGAGAGTGGCGAGGTTCTCAAGGTGACGGCAAGTTTCGGATGTGCCGGC
CGGGCAAATGAAGCCGCCAACCGGAACTTCGAGGATCTGGTCAAACGCGCCGATCTCTCGCTCTACGCTGCCAAGGCCTC
CGGCCGCAATTGCGTCGTTTCGGGGCCGATCGTGCCGGCGCAGGAGGAGCGCCGCAAGACGGCGTCCGGCGGTGGCTTTG
ATTCCCGCATATGA

Upstream 100 bases:

>100_bases
CTAGGGATCTGCCGCTTTATCAGTTTATTAAGAATTGCTGCTAAGATTGGCGTCAATTGGTCGCGCACGACCGACGTTTT
GAGCACCGTGGCGATTGGCG

Downstream 100 bases:

>100_bases
AAAACGCCGCATGTCGCAGCGGCGTTTTCGGGTATGGTTGTCGAAGAAGACTTAAAGCCTTTTGTTTTGTGCATGTCGTT
GTCCCGGAACCGCTTCACAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKFETIKRVILAAIMLPFVFMLIEGVVVIRASVNQYRDLEKDRQFADVLARGGSIAATEILSEIGATRLYLAHPSSKTAA
DMQKSRATLDDERLAFYASLPSRDTLDEGLAEELSILSLAYSRIVAARSAVDQGRYVGSDPGSIYWYAALKQLAVVDALS
PLISDPVLLEKSNQLMGIMLTYYGERLITGIGSRYLDQGVSARFPVELFVQGKVMIGEGMDHMVFHSAAPIVRDIVAYLG
RRDQVKANAITDAILAGSRPTPAVRDVWAAAQSERMTFLQQKMIEAARDIHETGENFSTRSHLHLTRILALCAGLLILAT
LVMVLAARGLRLIDRLSRDRETLVGELRNAAQTDLLTGLYNRRGFEFAASALLTQAEHGSRWISVVLFDLDHFKKINDIN
GHDAGDAVLRHVAGVARQNFRSFDLLVRHGGEEFLALLPDSTPDDAAIVAERVRLAIEAAEIPLESGEVLKVTASFGCAG
RANEAANRNFEDLVKRADLSLYAAKASGRNCVVSGPIVPAQEERRKTASGGGFDSRI

Sequences:

>Translated_537_residues
MKFETIKRVILAAIMLPFVFMLIEGVVVIRASVNQYRDLEKDRQFADVLARGGSIAATEILSEIGATRLYLAHPSSKTAA
DMQKSRATLDDERLAFYASLPSRDTLDEGLAEELSILSLAYSRIVAARSAVDQGRYVGSDPGSIYWYAALKQLAVVDALS
PLISDPVLLEKSNQLMGIMLTYYGERLITGIGSRYLDQGVSARFPVELFVQGKVMIGEGMDHMVFHSAAPIVRDIVAYLG
RRDQVKANAITDAILAGSRPTPAVRDVWAAAQSERMTFLQQKMIEAARDIHETGENFSTRSHLHLTRILALCAGLLILAT
LVMVLAARGLRLIDRLSRDRETLVGELRNAAQTDLLTGLYNRRGFEFAASALLTQAEHGSRWISVVLFDLDHFKKINDIN
GHDAGDAVLRHVAGVARQNFRSFDLLVRHGGEEFLALLPDSTPDDAAIVAERVRLAIEAAEIPLESGEVLKVTASFGCAG
RANEAANRNFEDLVKRADLSLYAAKASGRNCVVSGPIVPAQEERRKTASGGGFDSRI
>Mature_537_residues
MKFETIKRVILAAIMLPFVFMLIEGVVVIRASVNQYRDLEKDRQFADVLARGGSIAATEILSEIGATRLYLAHPSSKTAA
DMQKSRATLDDERLAFYASLPSRDTLDEGLAEELSILSLAYSRIVAARSAVDQGRYVGSDPGSIYWYAALKQLAVVDALS
PLISDPVLLEKSNQLMGIMLTYYGERLITGIGSRYLDQGVSARFPVELFVQGKVMIGEGMDHMVFHSAAPIVRDIVAYLG
RRDQVKANAITDAILAGSRPTPAVRDVWAAAQSERMTFLQQKMIEAARDIHETGENFSTRSHLHLTRILALCAGLLILAT
LVMVLAARGLRLIDRLSRDRETLVGELRNAAQTDLLTGLYNRRGFEFAASALLTQAEHGSRWISVVLFDLDHFKKINDIN
GHDAGDAVLRHVAGVARQNFRSFDLLVRHGGEEFLALLPDSTPDDAAIVAERVRLAIEAAEIPLESGEVLKVTASFGCAG
RANEAANRNFEDLVKRADLSLYAAKASGRNCVVSGPIVPAQEERRKTASGGGFDSRI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=211, Percent_Identity=36.4928909952607, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693134, Length=179, Percent_Identity=35.195530726257, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=37.2881355932203, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=272, Percent_Identity=29.7794117647059, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=34.4632768361582, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=36.5269461077844, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=33.5443037974684, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=30.4093567251462, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=31.7365269461078, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=29.375, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=30.635838150289, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 58723; Mature: 58723

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFETIKRVILAAIMLPFVFMLIEGVVVIRASVNQYRDLEKDRQFADVLARGGSIAATEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LSEIGATRLYLAHPSSKTAADMQKSRATLDDERLAFYASLPSRDTLDEGLAEELSILSLA
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHH
YSRIVAARSAVDQGRYVGSDPGSIYWYAALKQLAVVDALSPLISDPVLLEKSNQLMGIML
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TYYGERLITGIGSRYLDQGVSARFPVELFVQGKVMIGEGMDHMVFHSAAPIVRDIVAYLG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCCHHHHHHHCCHHHHHHHHHHC
RRDQVKANAITDAILAGSRPTPAVRDVWAAAQSERMTFLQQKMIEAARDIHETGENFSTR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SHLHLTRILALCAGLLILATLVMVLAARGLRLIDRLSRDRETLVGELRNAAQTDLLTGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRRGFEFAASALLTQAEHGSRWISVVLFDLDHFKKINDINGHDAGDAVLRHVAGVARQNF
HCCCHHHHHHHHHHHHHCCCCEEHHEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RSFDLLVRHGGEEFLALLPDSTPDDAAIVAERVRLAIEAAEIPLESGEVLKVTASFGCAG
HHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
RANEAANRNFEDLVKRADLSLYAAKASGRNCVVSGPIVPAQEERRKTASGGGFDSRI
CCCHHHCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKFETIKRVILAAIMLPFVFMLIEGVVVIRASVNQYRDLEKDRQFADVLARGGSIAATEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LSEIGATRLYLAHPSSKTAADMQKSRATLDDERLAFYASLPSRDTLDEGLAEELSILSLA
HHHHCCCEEEEECCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHHH
YSRIVAARSAVDQGRYVGSDPGSIYWYAALKQLAVVDALSPLISDPVLLEKSNQLMGIML
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
TYYGERLITGIGSRYLDQGVSARFPVELFVQGKVMIGEGMDHMVFHSAAPIVRDIVAYLG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCCHHHHHHHCCHHHHHHHHHHC
RRDQVKANAITDAILAGSRPTPAVRDVWAAAQSERMTFLQQKMIEAARDIHETGENFSTR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SHLHLTRILALCAGLLILATLVMVLAARGLRLIDRLSRDRETLVGELRNAAQTDLLTGLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRRGFEFAASALLTQAEHGSRWISVVLFDLDHFKKINDINGHDAGDAVLRHVAGVARQNF
HCCCHHHHHHHHHHHHHCCCCEEHHEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RSFDLLVRHGGEEFLALLPDSTPDDAAIVAERVRLAIEAAEIPLESGEVLKVTASFGCAG
HHHHHHHHCCCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
RANEAANRNFEDLVKRADLSLYAAKASGRNCVVSGPIVPAQEERRKTASGGGFDSRI
CCCHHHCCCHHHHHHHCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA