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The map label for this gene is ispG [H]

Identifier: 209551402

GI number: 209551402

Start: 3963288

End: 3964538

Strand: Reverse

Name: ispG [H]

Synonym: Rleg2_3831

Alternate gene names: 209551402

Gene position: 3964538-3963288 (Counterclockwise)

Preceding gene: 209551403

Following gene: 209551401

Centisome position: 87.36

GC content: 63.07

Gene sequence:

>1251_bases
ATGTCTCCAACTGCCGATTTTGATCCGAAACCGCGCCGCGCGTCCGTTGCCGTCGATGTCGGCGGCGTCATCGTCGGCGG
CGGGGCGCCGGTGGTCGTGCAATCGATGACGAACACTGACACGGCCGATATCGATTCGACCGTCGCCCAGGTCGCCGCTC
TCCACCGGGCGGGCTCGGAGCTGGTACGCATCACCGTCGACCGTGACGAGAGTGCGGCCGCCGTGCCCAAGATCCGCGAG
CGGCTGTTGCGGCTCGGCATGGACGTGCCATTGATCGGCGACTTCCATTATGTCGGCCACAAACTGCTTGCCGATCACCC
TGATTGTGCCGCAGCGCTCGCGAAATACCGCATCAATCCCGGCAATGTCGGCTTCAAGGACAAGAAGGACAAGCAGTTCG
CCGAGATCATCGAGATGGCGATCCGCTACGACAAGCCGGTGCGCGTCGGCGTCAACTGGGGTTCGCTCGATCAGGATCTC
TTGACGGCGCTGATGGATGAGAATGCTAGAGCCGGTTCGCCGCTTTCGGCCCGGCAGGTAACACGCGAGGCGATCGTGCA
ATCGGCGCTCCTTTCGGCAGCCCTTGCCGAAGAGATCGGCCTGCCGCGCAACCGCATCATCCTGTCGGCCAAGGTCAGCC
AGGTCCAGGACCTGATCGCCGTCAATTCCATGCTTGCCGAACGCTCCAATCATGCGCTTCATCTCGGCCTGACCGAAGCC
GGCATGGGCACCAAGGGCATCGTCGCCTCATCGGCGGCGATGGGTTTCGTGCTCCAGCACGGCATCGGCGATACGATCCG
CGTATCGCTGACGCCGGAGCCGAACGGCGACCGCACGCGCGAAGTTCAGGTAGCGCAGGAAATCCTGCAGGTCATGGGCT
TCCGCCAGTTCATTCCTGTCGTTGCCGCCTGTCCTGGCTGCGGACGCACGACGTCGACAGTGTTCCAGGAGCTTGCCCAG
AACATCCAGAACGACATCCGCAAGAACATGCCGGTCTGGCGCGAGAAATATCCGGGCGTCGAGGCGCTGAATGTTGCCGT
CATGGGCTGCATCGTCAACGGACCGGGCGAAAGCAAACATGCCGATATCGGCATTTCGCTTCCCGGCACCGGCGAGACGC
CGGCAGCCCCGGTCTTCATCGACGGGAAGAAGGCGCTGACATTGCGCGGTCCCAATATCGCTGCCGACTTCGAGGCGCTC
GTCGTCGACTATATCGAGAAGCGTTTCGGCCAGCGGACGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
TAAGCCGCCTCCCAACAAAAGTGATATCGGGAGCGGTTGACAAGAAAGCAGTCATGATCCACCTGAAAGGCACTGTTTTC
AGGGGTGCAGGAAACTCTTA

Downstream 100 bases:

>100_bases
AGGTCGGCGAATTCGCATGAGCCGCTACTGGTCTGATATCGTCAGCAAGCTCCGACCCTATGTCGCCGGGGAGCAACCCC
GTATCCCCGGTCTGGTCAAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSPTADFDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDSTVAQVAALHRAGSELVRITVDRDESAAAVPKIRE
RLLRLGMDVPLIGDFHYVGHKLLADHPDCAAALAKYRINPGNVGFKDKKDKQFAEIIEMAIRYDKPVRVGVNWGSLDQDL
LTALMDENARAGSPLSARQVTREAIVQSALLSAALAEEIGLPRNRIILSAKVSQVQDLIAVNSMLAERSNHALHLGLTEA
GMGTKGIVASSAAMGFVLQHGIGDTIRVSLTPEPNGDRTREVQVAQEILQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
NIQNDIRKNMPVWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGETPAAPVFIDGKKALTLRGPNIAADFEAL
VVDYIEKRFGQRTAAE

Sequences:

>Translated_416_residues
MSPTADFDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDSTVAQVAALHRAGSELVRITVDRDESAAAVPKIRE
RLLRLGMDVPLIGDFHYVGHKLLADHPDCAAALAKYRINPGNVGFKDKKDKQFAEIIEMAIRYDKPVRVGVNWGSLDQDL
LTALMDENARAGSPLSARQVTREAIVQSALLSAALAEEIGLPRNRIILSAKVSQVQDLIAVNSMLAERSNHALHLGLTEA
GMGTKGIVASSAAMGFVLQHGIGDTIRVSLTPEPNGDRTREVQVAQEILQVMGFRQFIPVVAACPGCGRTTSTVFQELAQ
NIQNDIRKNMPVWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGETPAAPVFIDGKKALTLRGPNIAADFEAL
VVDYIEKRFGQRTAAE
>Mature_415_residues
SPTADFDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDSTVAQVAALHRAGSELVRITVDRDESAAAVPKIRER
LLRLGMDVPLIGDFHYVGHKLLADHPDCAAALAKYRINPGNVGFKDKKDKQFAEIIEMAIRYDKPVRVGVNWGSLDQDLL
TALMDENARAGSPLSARQVTREAIVQSALLSAALAEEIGLPRNRIILSAKVSQVQDLIAVNSMLAERSNHALHLGLTEAG
MGTKGIVASSAAMGFVLQHGIGDTIRVSLTPEPNGDRTREVQVAQEILQVMGFRQFIPVVAACPGCGRTTSTVFQELAQN
IQNDIRKNMPVWREKYPGVEALNVAVMGCIVNGPGESKHADIGISLPGTGETPAAPVFIDGKKALTLRGPNIAADFEALV
VDYIEKRFGQRTAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=401, Percent_Identity=32.1695760598504, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44417; Mature: 44286

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTADFDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDSTVAQVAALHRAGSE
CCCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LVRITVDRDESAAAVPKIRERLLRLGMDVPLIGDFHYVGHKLLADHPDCAAALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHCCCCHHHHHHHHHCCCC
GNVGFKDKKDKQFAEIIEMAIRYDKPVRVGVNWGSLDQDLLTALMDENARAGSPLSARQV
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
TREAIVQSALLSAALAEEIGLPRNRIILSAKVSQVQDLIAVNSMLAERSNHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
GMGTKGIVASSAAMGFVLQHGIGDTIRVSLTPEPNGDRTREVQVAQEILQVMGFRQFIPV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQNIQNDIRKNMPVWREKYPGVEALNVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHEEEEEEEECCCCCCCC
ADIGISLPGTGETPAAPVFIDGKKALTLRGPNIAADFEALVVDYIEKRFGQRTAAE
CEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPTADFDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDSTVAQVAALHRAGSE
CCCCCCCCCCCCEEEEEEECCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCE
LVRITVDRDESAAAVPKIRERLLRLGMDVPLIGDFHYVGHKLLADHPDCAAALAKYRINP
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHCCCCHHHHHHHHHCCCC
GNVGFKDKKDKQFAEIIEMAIRYDKPVRVGVNWGSLDQDLLTALMDENARAGSPLSARQV
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
TREAIVQSALLSAALAEEIGLPRNRIILSAKVSQVQDLIAVNSMLAERSNHALHLGLTEA
HHHHHHHHHHHHHHHHHHHCCCHHEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHC
GMGTKGIVASSAAMGFVLQHGIGDTIRVSLTPEPNGDRTREVQVAQEILQVMGFRQFIPV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VAACPGCGRTTSTVFQELAQNIQNDIRKNMPVWREKYPGVEALNVAVMGCIVNGPGESKH
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHEEEEEEEECCCCCCCC
ADIGISLPGTGETPAAPVFIDGKKALTLRGPNIAADFEALVVDYIEKRFGQRTAAE
CEEEEECCCCCCCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA