Click here to switch to the map view.

The map label for this gene is dapF [H]

Identifier: 209551319

GI number: 209551319

Start: 3874359

End: 3875264

Strand: Reverse

Name: dapF [H]

Synonym: Rleg2_3748

Alternate gene names: 209551319

Gene position: 3875264-3874359 (Counterclockwise)

Preceding gene: 209551320

Following gene: 209551318

Centisome position: 85.4

GC content: 65.34

Gene sequence:

>906_bases
ATGAGCGCAACGGTCGAATTCGCGAGGATGAACGGGCTTGGCAACAAGATCCTGGTCGTCGACATGCGCGGCCGGCCCGA
TAAGGTGACGCCGGCGGCAGCGGTCGCACTGAATGCCGATCCGCAGACCGAGTTCGACCAGATCATGGCGATCCATGACC
CGAAGGCCGACGGCACCGACGCCTTTATCGACATCCTGAATTCCGACGGCTCGAAGGCGCAGGCCTGCGGCAACGGCACG
CGCTGCGTCGTGCAGGCGCTTGCCGCCGAGACCGGCCGCAAGGCCTTCACCTTCCAGACGGTCGCCGGCATCCTCAACGC
CGTCGAGCACGAGGACGGAACGATCTCCGTCGATATGGGCCGGCCGGTCTTCGATTGGAACAGGATCCCGCTGGCGGAAG
AATTTCACGATACCAGCCGCATCGAATTGCAGATCGGCCCGATCGATAATCCGGTGCTGCATTCGCCCTCGGCCATGTCG
ATGGGCAATCCGCATGCGATCTTCTGGGTCGACAGGGACGTGATGTCCTATGATCTCGCCCGCTTCGGGCCGCTGCTCGA
AAACCATCCGATGTTTCCCGAACGCGCCAATATCACCCTGGCGCAGGTGACCTCGCCGACATCGATGACGACGCGCACCT
GGGAGCGCGGCGCGGGGCTGACGCTTGCCTGCGGCTCGGCTGCCTGTTCTGCCGCGGTCAGTGCGGCGCGCACCGGCCGC
ACCGGCCGCAAGGTGACGATCAACGTGGCGAGCGCCAAGCCGCCGGCTACGCTTTCGATCGAATGGCGTGAGCGCGACGA
TCACGTGATCATGACCGGCCCGGCCGAATGGGAATGGTCGGGCAGTCTCGATCCCTCGACTGGCCTCTGGTCGCGCGACG
GTACCCAAGAGGCGGGGGCGCGGTGA

Upstream 100 bases:

>100_bases
TCGAAAGGGCGGCCGGCGGCAATGGCTGCGCGGCACTCACCCACTGGTAATTCCTTCGCATTTCCGCCATATACAGCGGA
AACACGGACGGAATGATACT

Downstream 100 bases:

>100_bases
GCGGCGTCGACGTCATTACCTTCGGCTGCCGCCTCAACACCTATGAATCCGAAGTGATGAAGGCGCAGGCCGAGAAGGCC
GGGCTCAACAATGCCATCCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSATVEFARMNGLGNKILVVDMRGRPDKVTPAAAVALNADPQTEFDQIMAIHDPKADGTDAFIDILNSDGSKAQACGNGT
RCVVQALAAETGRKAFTFQTVAGILNAVEHEDGTISVDMGRPVFDWNRIPLAEEFHDTSRIELQIGPIDNPVLHSPSAMS
MGNPHAIFWVDRDVMSYDLARFGPLLENHPMFPERANITLAQVTSPTSMTTRTWERGAGLTLACGSAACSAAVSAARTGR
TGRKVTINVASAKPPATLSIEWRERDDHVIMTGPAEWEWSGSLDPSTGLWSRDGTQEAGAR

Sequences:

>Translated_301_residues
MSATVEFARMNGLGNKILVVDMRGRPDKVTPAAAVALNADPQTEFDQIMAIHDPKADGTDAFIDILNSDGSKAQACGNGT
RCVVQALAAETGRKAFTFQTVAGILNAVEHEDGTISVDMGRPVFDWNRIPLAEEFHDTSRIELQIGPIDNPVLHSPSAMS
MGNPHAIFWVDRDVMSYDLARFGPLLENHPMFPERANITLAQVTSPTSMTTRTWERGAGLTLACGSAACSAAVSAARTGR
TGRKVTINVASAKPPATLSIEWRERDDHVIMTGPAEWEWSGSLDPSTGLWSRDGTQEAGAR
>Mature_300_residues
SATVEFARMNGLGNKILVVDMRGRPDKVTPAAAVALNADPQTEFDQIMAIHDPKADGTDAFIDILNSDGSKAQACGNGTR
CVVQALAAETGRKAFTFQTVAGILNAVEHEDGTISVDMGRPVFDWNRIPLAEEFHDTSRIELQIGPIDNPVLHSPSAMSM
GNPHAIFWVDRDVMSYDLARFGPLLENHPMFPERANITLAQVTSPTSMTTRTWERGAGLTLACGSAACSAAVSAARTGRT
GRKVTINVASAKPPATLSIEWRERDDHVIMTGPAEWEWSGSLDPSTGLWSRDGTQEAGAR

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=283, Percent_Identity=32.5088339222615, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 32388; Mature: 32257

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVEFARMNGLGNKILVVDMRGRPDKVTPAAAVALNADPQTEFDQIMAIHDPKADGTD
CCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEEECCCCCCCC
AFIDILNSDGSKAQACGNGTRCVVQALAAETGRKAFTFQTVAGILNAVEHEDGTISVDMG
CEEEEECCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEEEEECC
RPVFDWNRIPLAEEFHDTSRIELQIGPIDNPVLHSPSAMSMGNPHAIFWVDRDVMSYDLA
CCEECCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHH
RFGPLLENHPMFPERANITLAQVTSPTSMTTRTWERGAGLTLACGSAACSAAVSAARTGR
HHCCHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHCCC
TGRKVTINVASAKPPATLSIEWRERDDHVIMTGPAEWEWSGSLDPSTGLWSRDGTQEAGA
CCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHCCC
R
C
>Mature Secondary Structure 
SATVEFARMNGLGNKILVVDMRGRPDKVTPAAAVALNADPQTEFDQIMAIHDPKADGTD
CCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEEECCCCCCCC
AFIDILNSDGSKAQACGNGTRCVVQALAAETGRKAFTFQTVAGILNAVEHEDGTISVDMG
CEEEEECCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCEEEEECC
RPVFDWNRIPLAEEFHDTSRIELQIGPIDNPVLHSPSAMSMGNPHAIFWVDRDVMSYDLA
CCEECCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHH
RFGPLLENHPMFPERANITLAQVTSPTSMTTRTWERGAGLTLACGSAACSAAVSAARTGR
HHCCHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHCCC
TGRKVTINVASAKPPATLSIEWRERDDHVIMTGPAEWEWSGSLDPSTGLWSRDGTQEAGA
CCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA