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The map label for this gene is nuoE [H]

Identifier: 209551212

GI number: 209551212

Start: 3770157

End: 3770636

Strand: Reverse

Name: nuoE [H]

Synonym: Rleg2_3638

Alternate gene names: 209551212

Gene position: 3770636-3770157 (Counterclockwise)

Preceding gene: 209551213

Following gene: 209551211

Centisome position: 83.09

GC content: 63.12

Gene sequence:

>480_bases
ATGACCATTCATATCGCCGAAGGCGATATTGCCGCGCGCACTCGTGCCATCATTGCCGATCTTCGCTTCCTCGAAGGGTC
GCTGCTTCCCATTCTGCACGAGGTTCAGGACGAATTCGGCTATATCCCGCAGGAAGCCGTGCCGGTGATCGCGGAGGAAC
TGAACCTCTCCCGCGCCGAGGTGCATGGCGTCGTGACTTTCTATCACGATTACCGCGACCACCCGGCCGGCCGGCATGTG
CTGAAGCTTTGTCGCGCCGAGGCCTGTCAGTCGATGGGCGGCGATGCGCTGGCCGAGCGCGTCAAAACGCTGCTCGGCAT
CGATTTTCATCAGACGACGCTCGACGGCAGCGTAACGCTGGAGCCGGTCTACTGTCTCGGGCTCTGCGCCTGTGCCCCAT
CGGCGATGCTCGACGGCGAGGTCTATGGCCGGGTCGACGATCAGCTGGCGGCGGAACTCGTTGCGGAGGCACGCCGATGA

Upstream 100 bases:

>100_bases
TGATCGCCGGCGGCTTCCTTGAGATGCTGTCGGCGGAATAGCACTCGGGCTGGAGGAGGCCGCCATTCCGCAACGAGCAA
GCCGTCGGGAGGGCTGCCTT

Downstream 100 bases:

>100_bases
GCATCAAGATTTATGTTCCGCGCGATGCCGCTGCGCTGGCGCTCGGGGCCGAAAAGGTGGCAAAGGCGATTGCCCAGGAG
ATCGCCGCCCGCGGCCTCGA

Product: formate dehydrogenase subunit gamma

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTIHIAEGDIAARTRAIIADLRFLEGSLLPILHEVQDEFGYIPQEAVPVIAEELNLSRAEVHGVVTFYHDYRDHPAGRHV
LKLCRAEACQSMGGDALAERVKTLLGIDFHQTTLDGSVTLEPVYCLGLCACAPSAMLDGEVYGRVDDQLAAELVAEARR

Sequences:

>Translated_159_residues
MTIHIAEGDIAARTRAIIADLRFLEGSLLPILHEVQDEFGYIPQEAVPVIAEELNLSRAEVHGVVTFYHDYRDHPAGRHV
LKLCRAEACQSMGGDALAERVKTLLGIDFHQTTLDGSVTLEPVYCLGLCACAPSAMLDGEVYGRVDDQLAAELVAEARR
>Mature_158_residues
TIHIAEGDIAARTRAIIADLRFLEGSLLPILHEVQDEFGYIPQEAVPVIAEELNLSRAEVHGVVTFYHDYRDHPAGRHVL
KLCRAEACQSMGGDALAERVKTLLGIDFHQTTLDGSVTLEPVYCLGLCACAPSAMLDGEVYGRVDDQLAAELVAEARR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=150, Percent_Identity=30, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788621, Length=120, Percent_Identity=34.1666666666667, Blast_Score=81, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=117, Percent_Identity=32.4786324786325, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355353, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI18859877, Length=117, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17384; Mature: 17253

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHIAEGDIAARTRAIIADLRFLEGSLLPILHEVQDEFGYIPQEAVPVIAEELNLSRAE
CEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
VHGVVTFYHDYRDHPAGRHVLKLCRAEACQSMGGDALAERVKTLLGIDFHQTTLDGSVTL
HHHHHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHEECCCCEEE
EPVYCLGLCACAPSAMLDGEVYGRVDDQLAAELVAEARR
CHHHHHHHHHHCCCHHCCCHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIHIAEGDIAARTRAIIADLRFLEGSLLPILHEVQDEFGYIPQEAVPVIAEELNLSRAE
EEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
VHGVVTFYHDYRDHPAGRHVLKLCRAEACQSMGGDALAERVKTLLGIDFHQTTLDGSVTL
HHHHHHEEHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHEECCCCEEE
EPVYCLGLCACAPSAMLDGEVYGRVDDQLAAELVAEARR
CHHHHHHHHHHCCCHHCCCHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]