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The map label for this gene is ptsP [H]

Identifier: 209551101

GI number: 209551101

Start: 3654147

End: 3656414

Strand: Reverse

Name: ptsP [H]

Synonym: Rleg2_3525

Alternate gene names: 209551101

Gene position: 3656414-3654147 (Counterclockwise)

Preceding gene: 209551102

Following gene: 209551100

Centisome position: 80.57

GC content: 64.33

Gene sequence:

>2268_bases
ATGAGAGACCTTTCCGGCGGTCCGCGCGTGCTGCTCAGGCGGCTGCGCGAGTTGATGGCGGAGCCGCTGGAGCCGCAGGA
TCGTCTTGACCGGATCGTCCGCCAGATCGCCGGCAACATGGTGGCTGAGGTCTGCTCGGTTTACGTGCTGCGCGCCGATG
GCGTGCTCGAACTCTATGCCACCGAAGGCCTCAACCGCGAAGCCGTGCACCTGGCGCAATTGAAGATGGGGCAGGGCCTG
GTCGGCACGATCGCGGCCTCGGCCCAGCCGCTCAACCTTTCCGACGCACAGTCGCATCCTGCTTTCCGCTACCTGCCGGA
GACCGGCGAAGAGATCTACCATTCCTTCCTCGGTGTGCCGATCCTCAGGACCGGGCGCTCGCTCGGCGTTCTCGTCGTGC
AGAACAAGGCAAGCCGCAACTATCGCGAGGAGGAGCTCGAAGCGCTCGAGACGACGGCGATGGTGCTGGCCGAGATGATC
GCCACCGGCGAGCTGAAGAAGATCACCAAGCCGGGCCTCGAACTCGACCTGACGCGCTCGGTGACCGTCGACGGCGACAC
CTATAATGACGGCATCGGCCTCGGTTACGTCGTGCTGCACGAGCCGCGCATCGTCGTCACCAACCTGCTCAACGAAGATG
CCGAGAAGGAAATCCGGCGGCTGGCCGAAGCCATGGGCTCGCTCAGGATCTCGATCGACGACATGCTGTCGCGCCGCGAC
GTGTCAATGGAAGGCGAGCACCGCGAGGTGCTCGAAACCTATCGCATGTTCGCGCATGACCAGGGCTGGGTGCGCAAGCT
CGAGGAGGCGATCCGCAACGGCCTGACGGCGGAAGCGGCGGTCGAAAAGGTGCAGAGCGACACCAAGGCGCGGATGATGC
GGTTGACCGACCCCTATCTGCGCGAACGCATGCATGATTTCGAGGATCTGGCGAACCGGCTGCTCAGGCAATTGACGGGT
TATACCGGGCGGACGACCGCCGAGGGCTTCCCCAACGATGCGGTCATCCTGGCGCGCGCCATGGGGGCGGCCGAGCTGCT
CGATTATCCCCGCGCCAATGTGCGCGGGCTGGTGCTGGAAGAAGGCGCGGTGACCAGCCATGTGGTGATCGTCGCGCGCG
CCATGGGTATTCCGGTCATCGGCCAGGCAGCGGGTGTGGTGGCGCTTGCCGAGAACGGCGATGCCGTGATCATCGACGCC
GACGAAGGACACGTGCATCTGCGGCCGATGGCCGATCACCGGCGCTCCTACGAGGAAAAGGTTCGCTTCCGCGCCAGACG
GCAGGAACAGTTCCGAGCACTGCGCGCCGTCGAGCCGGTGACAAAGGATGGGCAGCGGATCGCGCTGATGATGAATGCGG
GGCTGCTGGTCGACCTGCCGCAGCTTTCGGAATCGGGTGCCGAGGGCATTGGCCTCTTCCGCACCGAACTGCAGTTCATG
ATCGCCTCCACCATGCCGAAGGCGGACGAGCAGGAGCAGTTCTACCGCAATGTGATAAAACAGGCGGCGGGCCGCCCCGT
CACCTTCCGTACGCTCGATATCGGCGGCGACAAGGTCGTGCCGTATTTCCGCGGCCATGAGGAAGAAAATCCAGCACTCG
GCTGGCGCGCCATCCGGCTGTCGCTCGACCGGCCGGGACTGTTGCGCACCCAGTTGCGCGCCCTGTTGAAGGCATCGGCC
GATACCGAGCTGAAGCTGATGGTGCCGATGGTAACCGAGGTTTCCGAGCTGAAGGTGGTGCGCGAGCTGCTGCAGAAGGA
AGTGCAGCATCTCTCACGCTTCGGCCATGGTCTGCCGCGCAAGCTGCAGTTCGGAGCGATGCTGGAGGTGCCAGCACTGC
TTTGGCAGCTCGATGAGCTGATGGAGGCGGTGGATTTCGTCTCGGTCGGTTCGAACGACCTCTTCCAGTTTTCGATGGCG
GTCGATCGCGGCAATGCGCGGGTCTCGGACCGGTTCGACCCGCTCGGCAAACCGTTTTTGCGCATTCTGCGCGATATCGT
GCGCGGCGCGGACCGGAACAAGACGCCGGTAACGCTCTGCGGCGAACTCGCCAGCAAGCCGATCTCGGCGATGGCGCTGC
TCGGTATAGGTTTCCGTTCGATCTCGATGTCGCCGGCCTCGATCGGACCGGTGAAGGCGATGCTGCTCGGGCTCGATGTC
GGGGCGCTGGCAACGGCGATGAACGAGGTGCTGGACGATGTCCACGCCATGACGCCGATGCGAGAGGTGCTTGCCCGCTT
CGCCGAGAGCCACAATATTCCCCTTTAG

Upstream 100 bases:

>100_bases
ATAAGAATTGATTCCCAGTGGGCGGCCTGCCCCTTCAGGTTGAGGAGACGAGGGCCGGCCCGCCGGGACTGAGTGCTTTG
TTTCGGGAGCTTGAGACGCC

Downstream 100 bases:

>100_bases
CTAGACGTTAAGAAGAATCGGAGTGAAGGGTGGCGAAGCTTCCCGTTGAAAAGATGCGCGAGCTGGAACGCCGTTTCGGC
GAGATCGAAGCGCGCATGTC

Product: PTSINtr with GAF domain, PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MRDLSGGPRVLLRRLRELMAEPLEPQDRLDRIVRQIAGNMVAEVCSVYVLRADGVLELYATEGLNREAVHLAQLKMGQGL
VGTIAASAQPLNLSDAQSHPAFRYLPETGEEIYHSFLGVPILRTGRSLGVLVVQNKASRNYREEELEALETTAMVLAEMI
ATGELKKITKPGLELDLTRSVTVDGDTYNDGIGLGYVVLHEPRIVVTNLLNEDAEKEIRRLAEAMGSLRISIDDMLSRRD
VSMEGEHREVLETYRMFAHDQGWVRKLEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYLRERMHDFEDLANRLLRQLTG
YTGRTTAEGFPNDAVILARAMGAAELLDYPRANVRGLVLEEGAVTSHVVIVARAMGIPVIGQAAGVVALAENGDAVIIDA
DEGHVHLRPMADHRRSYEEKVRFRARRQEQFRALRAVEPVTKDGQRIALMMNAGLLVDLPQLSESGAEGIGLFRTELQFM
IASTMPKADEQEQFYRNVIKQAAGRPVTFRTLDIGGDKVVPYFRGHEEENPALGWRAIRLSLDRPGLLRTQLRALLKASA
DTELKLMVPMVTEVSELKVVRELLQKEVQHLSRFGHGLPRKLQFGAMLEVPALLWQLDELMEAVDFVSVGSNDLFQFSMA
VDRGNARVSDRFDPLGKPFLRILRDIVRGADRNKTPVTLCGELASKPISAMALLGIGFRSISMSPASIGPVKAMLLGLDV
GALATAMNEVLDDVHAMTPMREVLARFAESHNIPL

Sequences:

>Translated_755_residues
MRDLSGGPRVLLRRLRELMAEPLEPQDRLDRIVRQIAGNMVAEVCSVYVLRADGVLELYATEGLNREAVHLAQLKMGQGL
VGTIAASAQPLNLSDAQSHPAFRYLPETGEEIYHSFLGVPILRTGRSLGVLVVQNKASRNYREEELEALETTAMVLAEMI
ATGELKKITKPGLELDLTRSVTVDGDTYNDGIGLGYVVLHEPRIVVTNLLNEDAEKEIRRLAEAMGSLRISIDDMLSRRD
VSMEGEHREVLETYRMFAHDQGWVRKLEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYLRERMHDFEDLANRLLRQLTG
YTGRTTAEGFPNDAVILARAMGAAELLDYPRANVRGLVLEEGAVTSHVVIVARAMGIPVIGQAAGVVALAENGDAVIIDA
DEGHVHLRPMADHRRSYEEKVRFRARRQEQFRALRAVEPVTKDGQRIALMMNAGLLVDLPQLSESGAEGIGLFRTELQFM
IASTMPKADEQEQFYRNVIKQAAGRPVTFRTLDIGGDKVVPYFRGHEEENPALGWRAIRLSLDRPGLLRTQLRALLKASA
DTELKLMVPMVTEVSELKVVRELLQKEVQHLSRFGHGLPRKLQFGAMLEVPALLWQLDELMEAVDFVSVGSNDLFQFSMA
VDRGNARVSDRFDPLGKPFLRILRDIVRGADRNKTPVTLCGELASKPISAMALLGIGFRSISMSPASIGPVKAMLLGLDV
GALATAMNEVLDDVHAMTPMREVLARFAESHNIPL
>Mature_755_residues
MRDLSGGPRVLLRRLRELMAEPLEPQDRLDRIVRQIAGNMVAEVCSVYVLRADGVLELYATEGLNREAVHLAQLKMGQGL
VGTIAASAQPLNLSDAQSHPAFRYLPETGEEIYHSFLGVPILRTGRSLGVLVVQNKASRNYREEELEALETTAMVLAEMI
ATGELKKITKPGLELDLTRSVTVDGDTYNDGIGLGYVVLHEPRIVVTNLLNEDAEKEIRRLAEAMGSLRISIDDMLSRRD
VSMEGEHREVLETYRMFAHDQGWVRKLEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYLRERMHDFEDLANRLLRQLTG
YTGRTTAEGFPNDAVILARAMGAAELLDYPRANVRGLVLEEGAVTSHVVIVARAMGIPVIGQAAGVVALAENGDAVIIDA
DEGHVHLRPMADHRRSYEEKVRFRARRQEQFRALRAVEPVTKDGQRIALMMNAGLLVDLPQLSESGAEGIGLFRTELQFM
IASTMPKADEQEQFYRNVIKQAAGRPVTFRTLDIGGDKVVPYFRGHEEENPALGWRAIRLSLDRPGLLRTQLRALLKASA
DTELKLMVPMVTEVSELKVVRELLQKEVQHLSRFGHGLPRKLQFGAMLEVPALLWQLDELMEAVDFVSVGSNDLFQFSMA
VDRGNARVSDRFDPLGKPFLRILRDIVRGADRNKTPVTLCGELASKPISAMALLGIGFRSISMSPASIGPVKAMLLGLDV
GALATAMNEVLDDVHAMTPMREVLARFAESHNIPL

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=754, Percent_Identity=33.1564986737401, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1788756, Length=540, Percent_Identity=30.5555555555556, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI48994992, Length=488, Percent_Identity=32.7868852459016, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1788726, Length=544, Percent_Identity=32.5367647058824, Blast_Score=259, Evalue=6e-70,
Organism=Escherichia coli, GI1787994, Length=400, Percent_Identity=25.75, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83757; Mature: 83757

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00742 PEP_ENZYMES_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLSGGPRVLLRRLRELMAEPLEPQDRLDRIVRQIAGNMVAEVCSVYVLRADGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEHEECCCEEEEEE
TEGLNREAVHLAQLKMGQGLVGTIAASAQPLNLSDAQSHPAFRYLPETGEEIYHSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
ILRTGRSLGVLVVQNKASRNYREEELEALETTAMVLAEMIATGELKKITKPGLELDLTRS
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEE
VTVDGDTYNDGIGLGYVVLHEPRIVVTNLLNEDAEKEIRRLAEAMGSLRISIDDMLSRRD
EEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEHHHHHHHCC
VSMEGEHREVLETYRMFAHDQGWVRKLEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERMHDFEDLANRLLRQLTGYTGRTTAEGFPNDAVILARAMGAAELLDYPRANVRGLVLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCCCCEEEEE
EGAVTSHVVIVARAMGIPVIGQAAGVVALAENGDAVIIDADEGHVHLRPMADHRRSYEEK
CCCCHHHHHHHHHHHCCCEECCCCCEEEEECCCCEEEEECCCCCEEEEECHHHHHHHHHH
VRFRARRQEQFRALRAVEPVTKDGQRIALMMNAGLLVDLPQLSESGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECHHHCCCCCCCCHHHHHHHHHH
IASTMPKADEQEQFYRNVIKQAAGRPVTFRTLDIGGDKVVPYFRGHEEENPALGWRAIRL
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEE
SLDRPGLLRTQLRALLKASADTELKLMVPMVTEVSELKVVRELLQKEVQHLSRFGHGLPR
ECCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLQFGAMLEVPALLWQLDELMEAVDFVSVGSNDLFQFSMAVDRGNARVSDRFDPLGKPFL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHH
RILRDIVRGADRNKTPVTLCGELASKPISAMALLGIGFRSISMSPASIGPVKAMLLGLDV
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHCCH
GALATAMNEVLDDVHAMTPMREVLARFAESHNIPL
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRDLSGGPRVLLRRLRELMAEPLEPQDRLDRIVRQIAGNMVAEVCSVYVLRADGVLELYA
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEHEECCCEEEEEE
TEGLNREAVHLAQLKMGQGLVGTIAASAQPLNLSDAQSHPAFRYLPETGEEIYHSFLGVP
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCCH
ILRTGRSLGVLVVQNKASRNYREEELEALETTAMVLAEMIATGELKKITKPGLELDLTRS
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEEE
VTVDGDTYNDGIGLGYVVLHEPRIVVTNLLNEDAEKEIRRLAEAMGSLRISIDDMLSRRD
EEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHCEEEEHHHHHHHCC
VSMEGEHREVLETYRMFAHDQGWVRKLEEAIRNGLTAEAAVEKVQSDTKARMMRLTDPYL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
RERMHDFEDLANRLLRQLTGYTGRTTAEGFPNDAVILARAMGAAELLDYPRANVRGLVLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCCCCEEEEE
EGAVTSHVVIVARAMGIPVIGQAAGVVALAENGDAVIIDADEGHVHLRPMADHRRSYEEK
CCCCHHHHHHHHHHHCCCEECCCCCEEEEECCCCEEEEECCCCCEEEEECHHHHHHHHHH
VRFRARRQEQFRALRAVEPVTKDGQRIALMMNAGLLVDLPQLSESGAEGIGLFRTELQFM
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEECHHHCCCCCCCCHHHHHHHHHH
IASTMPKADEQEQFYRNVIKQAAGRPVTFRTLDIGGDKVVPYFRGHEEENPALGWRAIRL
HHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCEEEEEE
SLDRPGLLRTQLRALLKASADTELKLMVPMVTEVSELKVVRELLQKEVQHLSRFGHGLPR
ECCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLQFGAMLEVPALLWQLDELMEAVDFVSVGSNDLFQFSMAVDRGNARVSDRFDPLGKPFL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHH
RILRDIVRGADRNKTPVTLCGELASKPISAMALLGIGFRSISMSPASIGPVKAMLLGLDV
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHCCH
GALATAMNEVLDDVHAMTPMREVLARFAESHNIPL
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]