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The map label for this gene is lysC [H]

Identifier: 209551102

GI number: 209551102

Start: 3656504

End: 3657778

Strand: Reverse

Name: lysC [H]

Synonym: Rleg2_3526

Alternate gene names: 209551102

Gene position: 3657778-3656504 (Counterclockwise)

Preceding gene: 209551104

Following gene: 209551101

Centisome position: 80.6

GC content: 61.02

Gene sequence:

>1275_bases
ATGGCACGCATCGTAATGAAATTCGGCGGCACGTCCGTCGCTGACCTGGACCGCATCAAGAACGTCGCCCGCCATGTCAA
ACGCGAAGTCGACGCCGGCCACGAGGTGGCGGTGGTGGTGTCGGCCATGTCCGGCAAGACCAATGAGCTGGTCGGCTGGG
TGCAAGGCATGCCGAAGGTGATCGGCGCCAATTCGCCGTTTTACGATGCGCGGGAATATGATGCGGTGGTCGCCTCCGGC
GAGCAGGTAACCTCCGGGCTGCTGGCGATCGCTTTGCAGGCGATGGATATCAATGCGCGGTCCTGGCAGGGATGGCAGAT
CCCGATCCGCACCGATAATGCGCATGGCGCGGCGCGGATTATGGAGATCGACGGCTCCGACATCGTCAAGCGGATGGGCG
AGGGGCAGGTTGCCGTCATCGCCGGCTTCCAGGGACTGGGACCCGACAACCGCCTCGCGACGCTTGGCCGCGGCGGGTCG
GACACGTCGGCTGTCGCGATCGCAGCCGCTGTCAAGGCGGATCGCTGCGATATCTATACTGATGTCGACGGCGTCTACAC
GACCGACCCGCGCATCGTGCCGAAGGCGCGCAGGCTGAAGAAGATCGCCTTCGAGGAAATGCTGGAAATGGCCTCGCTCG
GCGCCAAGGTGCTGCAGGTGCGCTCGGTCGAGCTTGCCATGGTGCACAAGGTGCGCACCTTCGTGCGCTCGTCATTCGAA
GATCCCGATGCTCCGGGCATGGGCGATCTTCTAAACCCGCCCGGAACGCTGATTTGTGACGAGGATGAAATCGTGGAACA
GGAAGTAGTCACCGGCATCGCCTATGCCAAGGATGAGGCTCAGATCTCGCTTCGCCGTCTTGCCGACCGGCCGGGCGTCT
CCGCGGCGATCTTCGGGCCGCTCGCCGAATCCCATATCAATGTCGACATGATCGTCCAGAATATCTCTGAGGACGGGTCG
AAGACCGACATGACCTTCACCGTGCCGTCTGGCGACGTCGAGAAGGCGATCAAGGTGCTTAGCGACCATAAGGAGAAGAT
CGGCTACGATGTCGTGCAGAACGAATCGGGGCTGGTGAAAGTCTCGGTCATCGGCATCGGCATGCGCAGCCACGCCGGCG
TTGCCGCTACCGCATTTCGTGCACTTGCCGAAAAAGGCATCAACATCAAGGCGATCACCACCTCCGAGATCAAGATTTCC
ATCCTGATCGACGGTCCTTATGCGGAACTCGCTGTCAGGACTTTGCATTCCTGCTACGGTCTGGATAAGAATTGA

Upstream 100 bases:

>100_bases
AAGTCGGGAGCCGGCTCTTTGGCCAAGCGGTCCGCCCCAGAGCTTTCGTGATGCCGGGTCCGGTTTTCCGCTTTCCCGGC
GCCAGTCGTGGTAGAGGCAT

Downstream 100 bases:

>100_bases
TTCCCAGTGGGCGGCCTGCCCCTTCAGGTTGAGGAGACGAGGGCCGGCCCGCCGGGACTGAGTGCTTTGTTTCGGGAGCT
TGAGACGCCATGAGAGACCT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MARIVMKFGGTSVADLDRIKNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVQGMPKVIGANSPFYDAREYDAVVASG
EQVTSGLLAIALQAMDINARSWQGWQIPIRTDNAHGAARIMEIDGSDIVKRMGEGQVAVIAGFQGLGPDNRLATLGRGGS
DTSAVAIAAAVKADRCDIYTDVDGVYTTDPRIVPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
DPDAPGMGDLLNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAESHINVDMIVQNISEDGS
KTDMTFTVPSGDVEKAIKVLSDHKEKIGYDVVQNESGLVKVSVIGIGMRSHAGVAATAFRALAEKGINIKAITTSEIKIS
ILIDGPYAELAVRTLHSCYGLDKN

Sequences:

>Translated_424_residues
MARIVMKFGGTSVADLDRIKNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVQGMPKVIGANSPFYDAREYDAVVASG
EQVTSGLLAIALQAMDINARSWQGWQIPIRTDNAHGAARIMEIDGSDIVKRMGEGQVAVIAGFQGLGPDNRLATLGRGGS
DTSAVAIAAAVKADRCDIYTDVDGVYTTDPRIVPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
DPDAPGMGDLLNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAESHINVDMIVQNISEDGS
KTDMTFTVPSGDVEKAIKVLSDHKEKIGYDVVQNESGLVKVSVIGIGMRSHAGVAATAFRALAEKGINIKAITTSEIKIS
ILIDGPYAELAVRTLHSCYGLDKN
>Mature_423_residues
ARIVMKFGGTSVADLDRIKNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVQGMPKVIGANSPFYDAREYDAVVASGE
QVTSGLLAIALQAMDINARSWQGWQIPIRTDNAHGAARIMEIDGSDIVKRMGEGQVAVIAGFQGLGPDNRLATLGRGGSD
TSAVAIAAAVKADRCDIYTDVDGVYTTDPRIVPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFED
PDAPGMGDLLNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGPLAESHINVDMIVQNISEDGSK
TDMTFTVPSGDVEKAIKVLSDHKEKIGYDVVQNESGLVKVSVIGIGMRSHAGVAATAFRALAEKGINIKAITTSEIKISI
LIDGPYAELAVRTLHSCYGLDKN

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=474, Percent_Identity=29.957805907173, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1790455, Length=468, Percent_Identity=30.3418803418803, Blast_Score=135, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6320893, Length=300, Percent_Identity=30, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45383; Mature: 45251

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVMKFGGTSVADLDRIKNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVQGMPKV
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCHH
IGANSPFYDAREYDAVVASGEQVTSGLLAIALQAMDINARSWQGWQIPIRTDNAHGAARI
HCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEEEECCCCCCEEE
MEIDGSDIVKRMGEGQVAVIAGFQGLGPDNRLATLGRGGSDTSAVAIAAAVKADRCDIYT
EEECCHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEEEEHHCCCCCCEEE
DVDGVYTTDPRIVPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPDAPGMGDLLNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGP
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECC
LAESHINVDMIVQNISEDGSKTDMTFTVPSGDVEKAIKVLSDHKEKIGYDVVQNESGLVK
HHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEE
VSVIGIGMRSHAGVAATAFRALAEKGINIKAITTSEIKISILIDGPYAELAVRTLHSCYG
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHC
LDKN
CCCC
>Mature Secondary Structure 
ARIVMKFGGTSVADLDRIKNVARHVKREVDAGHEVAVVVSAMSGKTNELVGWVQGMPKV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCHH
IGANSPFYDAREYDAVVASGEQVTSGLLAIALQAMDINARSWQGWQIPIRTDNAHGAARI
HCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCCEEEEEEECCCCCCEEE
MEIDGSDIVKRMGEGQVAVIAGFQGLGPDNRLATLGRGGSDTSAVAIAAAVKADRCDIYT
EEECCHHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCCHHEEEEEHHCCCCCCEEE
DVDGVYTTDPRIVPKARRLKKIAFEEMLEMASLGAKVLQVRSVELAMVHKVRTFVRSSFE
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPDAPGMGDLLNPPGTLICDEDEIVEQEVVTGIAYAKDEAQISLRRLADRPGVSAAIFGP
CCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECC
LAESHINVDMIVQNISEDGSKTDMTFTVPSGDVEKAIKVLSDHKEKIGYDVVQNESGLVK
HHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCEEE
VSVIGIGMRSHAGVAATAFRALAEKGINIKAITTSEIKISILIDGPYAELAVRTLHSCYG
EEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHC
LDKN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]