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The map label for this gene is prfA [H]

Identifier: 209551100

GI number: 209551100

Start: 3653038

End: 3654117

Strand: Reverse

Name: prfA [H]

Synonym: Rleg2_3524

Alternate gene names: 209551100

Gene position: 3654117-3653038 (Counterclockwise)

Preceding gene: 209551101

Following gene: 209551099

Centisome position: 80.52

GC content: 62.04

Gene sequence:

>1080_bases
GTGGCGAAGCTTCCCGTTGAAAAGATGCGCGAGCTGGAACGCCGTTTCGGCGAGATCGAAGCGCGCATGTCGGCCGGCCC
GGCTGCCGATGTCTATGTGAAGCTCGCATCCGAATATTCCGAGCTGCAGCCGGTCGTGACGAAGATCCGCGCCTATGAAA
AGGCGATTGCCGAGCTTGCCGATCTCGAAACGCTCCTCGAGGACAAGTCGGTCGATCGCGAAATGCGCGACCTCGCCGAG
CTGGAGCTGCCCGAGGTGAAAGAGCAGATCGAGGCGCTCGAGCAGGAGATGCAGATTCTGCTTCTGCCGAAGGACGCAGC
GGACGAAAAAAGCGCGATCCTCGAAATCCGCGCCGGCACGGGCGGATCGGAAGCGGCCCTTTTTGCCGGCGATCTCTTTC
GTATGTATGAGCGTTTTTCGGCGGAAAAAGGCTGGAAGGTGGAGGTTCTCTCAGCAAGCGAAGGCGAAGCCGGCGGTTAC
AAGGAAATCATCGCGACGATCACCGGCAGAGGCGTCTTCGCCAAGCTGAAATTCGAATCCGGCGTGCATCGCGTGCAGCG
TGTGCCTGAGACCGAGGCGGGCGGGCGCATCCATACGTCGGCCGCAACGGTTGCAGTGCTGCCGGAGGCCGAGGAGATCG
ACATCGAGATCCGGCCGGAAGATATCCGCATCGACACGATGCGCTCTTCGGGCGCGGGCGGCCAGCACGTCAATACGACG
GACTCGGCGGTGCGCATCACCCATCTGCCGAGCGGCATCGTTGTCACCAGCTCGGAAAAATCGCAGCACCAGAACCGCGC
CAAGGCGATGCAAGTGCTGCGCTCCAGGCTCTACGACGCCGAGCGGCAGAGGGCCGACAGCGAGCGCTCGGCCGACCGCA
AGAGCCAGGTCGGTTCCGGCGACCGCTCCGAACGCATTCGCACCTATAATTTCCCGCAGGGGCGCATAACCGATCATCGC
ATCAATCTGACGCTCTATAAGCTCGACCGGATGATGGAAGGTGAGATCGAGGAAGTCGTCGATGCGCTGATGGCCGACTA
CCAGGCAAGCCAGCTGGCGCAGCTCGGCGAGCAGCAATGA

Upstream 100 bases:

>100_bases
ATGTCCACGCCATGACGCCGATGCGAGAGGTGCTTGCCCGCTTCGCCGAGAGCCACAATATTCCCCTTTAGCTAGACGTT
AAGAAGAATCGGAGTGAAGG

Downstream 100 bases:

>100_bases
GTTCGACGGTCGCCGATACGCTCGCCGAAGCGCGCCGCCGCTTCACGGAGGCGGGTATCGCCGACCCGGCGACCGATGCG
CGCTTGCTCGTCGCCGGCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAKLPVEKMRELERRFGEIEARMSAGPAADVYVKLASEYSELQPVVTKIRAYEKAIAELADLETLLEDKSVDREMRDLAE
LELPEVKEQIEALEQEMQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERFSAEKGWKVEVLSASEGEAGGY
KEIIATITGRGVFAKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRSSGAGGQHVNTT
DSAVRITHLPSGIVVTSSEKSQHQNRAKAMQVLRSRLYDAERQRADSERSADRKSQVGSGDRSERIRTYNFPQGRITDHR
INLTLYKLDRMMEGEIEEVVDALMADYQASQLAQLGEQQ

Sequences:

>Translated_359_residues
MAKLPVEKMRELERRFGEIEARMSAGPAADVYVKLASEYSELQPVVTKIRAYEKAIAELADLETLLEDKSVDREMRDLAE
LELPEVKEQIEALEQEMQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERFSAEKGWKVEVLSASEGEAGGY
KEIIATITGRGVFAKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRSSGAGGQHVNTT
DSAVRITHLPSGIVVTSSEKSQHQNRAKAMQVLRSRLYDAERQRADSERSADRKSQVGSGDRSERIRTYNFPQGRITDHR
INLTLYKLDRMMEGEIEEVVDALMADYQASQLAQLGEQQ
>Mature_358_residues
AKLPVEKMRELERRFGEIEARMSAGPAADVYVKLASEYSELQPVVTKIRAYEKAIAELADLETLLEDKSVDREMRDLAEL
ELPEVKEQIEALEQEMQILLLPKDAADEKSAILEIRAGTGGSEAALFAGDLFRMYERFSAEKGWKVEVLSASEGEAGGYK
EIIATITGRGVFAKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRSSGAGGQHVNTTD
SAVRITHLPSGIVVTSSEKSQHQNRAKAMQVLRSRLYDAERQRADSERSADRKSQVGSGDRSERIRTYNFPQGRITDHRI
NLTLYKLDRMMEGEIEEVVDALMADYQASQLAQLGEQQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=297, Percent_Identity=48.8215488215488, Blast_Score=284, Evalue=7e-77,
Organism=Homo sapiens, GI34577120, Length=329, Percent_Identity=40.4255319148936, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=50.5154639175258, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=54.5961002785515, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=314, Percent_Identity=38.5350318471338, Blast_Score=198, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=271, Percent_Identity=39.4833948339483, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=365, Percent_Identity=40.8219178082192, Blast_Score=252, Evalue=7e-68,
Organism=Drosophila melanogaster, GI19921226, Length=341, Percent_Identity=39.5894428152493, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40008; Mature: 39876

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLPVEKMRELERRFGEIEARMSAGPAADVYVKLASEYSELQPVVTKIRAYEKAIAELA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLETLLEDKSVDREMRDLAELELPEVKEQIEALEQEMQILLLPKDAADEKSAILEIRAGT
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERFSAEKGWKVEVLSASEGEAGGYKEIIATITGRGVFAKLKFES
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRSSGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPSGIVVTSSEKSQHQNRAKAMQVLRSRLYDAERQRADSERSADRKSQVGSG
CCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
DRSERIRTYNFPQGRITDHRINLTLYKLDRMMEGEIEEVVDALMADYQASQLAQLGEQQ
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKLPVEKMRELERRFGEIEARMSAGPAADVYVKLASEYSELQPVVTKIRAYEKAIAELA
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLETLLEDKSVDREMRDLAELELPEVKEQIEALEQEMQILLLPKDAADEKSAILEIRAGT
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCC
GGSEAALFAGDLFRMYERFSAEKGWKVEVLSASEGEAGGYKEIIATITGRGVFAKLKFES
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEEIDIEIRPEDIRIDTMRSSGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHLPSGIVVTSSEKSQHQNRAKAMQVLRSRLYDAERQRADSERSADRKSQVGSG
CCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
DRSERIRTYNFPQGRITDHRINLTLYKLDRMMEGEIEEVVDALMADYQASQLAQLGEQQ
CCCCCCEEECCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA