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The map label for this gene is afr [H]

Identifier: 209551044

GI number: 209551044

Start: 3581555

End: 3582592

Strand: Direct

Name: afr [H]

Synonym: Rleg2_3468

Alternate gene names: 209551044

Gene position: 3581555-3582592 (Clockwise)

Preceding gene: 209551043

Following gene: 209551054

Centisome position: 78.92

GC content: 59.06

Gene sequence:

>1038_bases
ATGGTCGACCACGTCAAACTAGCAGTCCTCGGCGCGGGACTGATTGGGAAGCGTCACATTCGACATGTCTTGGAAGAACC
CGCGGCGGACCTCGTCGCCGTGGTGGATCCCACATCGGTCGGTCAAAAAATTGCAGAGGAGGCCGGCGTCAGATGGTTTC
CGAGCTTTGCCGACATGATCGCCGTCCAACGTCCGGACGGCGTCATCATCGCCACCCCCAACCAAGTGCATGTCTCGAAC
GGCATGGAGGCTATAGAAGCCGGCATCCCGGCACTGATTGAGAAGCCGGTTGCCGATGACATCGCAGACGGCGAGAAACT
CATAGAGACGGCAAAAGCCAAGGGGGTCCCGCTTCTGACGGGCCATCATCGACGGCATAATCCGATGATGCAGAAAGCAA
AACAGATCATTGAAAGCGGGAGGCTCGGTCGGATACTGGTCGTCAACGCAATGTTCTGGCTGTTCAAGCCGGACGACTAT
TTCGACATGCCCTGGCGACGGGAGCGCGGCGCGGGACCTGTGTTTCTCAATCTGATCCACGACATCGACAATCTTCGCTA
CCTGTTTGGGGATATCGCGGCCGTCGAGGCGCGCGAGTCCAACGTAATGCGTGGAAACGCCGTCGAGGAGACAGCCGTGA
TCCTAATCGAATTCAAGAACGGAATTCTCGGCACTGCGTCGGTTTCGGATTCCGTTGTCGCGCCCTGGAGCTGGGAGATG
ACGACCGGAGAGAACGCCGCCTATCCGAAGACGGAAGAGGCCTGCTACATGATAGGCGGAACACACGGCTCGCTCGCCGT
TCCTTCGCTCGACATATGGTGCAACCCAGCGGAGCGGAGTTGGCTGGAGCCCTTCGACCGGAAACGCATCAGCGTCGAGA
CCGAGGATCCGCTTGTCCTTCAGATACGGCAGTTCTGCAAGGTCATCCGAGGTCACGAGGAGCCTCTGGTCAGCGGGCGA
GAGGGACTTGAAACCCTGAAGGTAATCGAAGCCGTCAAACGGTCCGCAGTCACGGCTCGGCGCGTCACGCTGTCGTGA

Upstream 100 bases:

>100_bases
GGCCGACGGTGTAATCGCCGGACTTGGGACGGAAGGCTACCAGCTCGGCATCCGCCGCGTCGCCACAATGCTAAAGACCC
CGAAGAACGGCTGACACAAA

Downstream 100 bases:

>100_bases
ATCGCGACACGTGCCTGCCCTCGTCTTAGGCTCGGAGTTCGAGACGACAATCGCATCGTCCGCCGGAATTCAAAGCGGAG
GAAGCAGGCGGCGCGCGTTT

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MVDHVKLAVLGAGLIGKRHIRHVLEEPAADLVAVVDPTSVGQKIAEEAGVRWFPSFADMIAVQRPDGVIIATPNQVHVSN
GMEAIEAGIPALIEKPVADDIADGEKLIETAKAKGVPLLTGHHRRHNPMMQKAKQIIESGRLGRILVVNAMFWLFKPDDY
FDMPWRRERGAGPVFLNLIHDIDNLRYLFGDIAAVEARESNVMRGNAVEETAVILIEFKNGILGTASVSDSVVAPWSWEM
TTGENAAYPKTEEACYMIGGTHGSLAVPSLDIWCNPAERSWLEPFDRKRISVETEDPLVLQIRQFCKVIRGHEEPLVSGR
EGLETLKVIEAVKRSAVTARRVTLS

Sequences:

>Translated_345_residues
MVDHVKLAVLGAGLIGKRHIRHVLEEPAADLVAVVDPTSVGQKIAEEAGVRWFPSFADMIAVQRPDGVIIATPNQVHVSN
GMEAIEAGIPALIEKPVADDIADGEKLIETAKAKGVPLLTGHHRRHNPMMQKAKQIIESGRLGRILVVNAMFWLFKPDDY
FDMPWRRERGAGPVFLNLIHDIDNLRYLFGDIAAVEARESNVMRGNAVEETAVILIEFKNGILGTASVSDSVVAPWSWEM
TTGENAAYPKTEEACYMIGGTHGSLAVPSLDIWCNPAERSWLEPFDRKRISVETEDPLVLQIRQFCKVIRGHEEPLVSGR
EGLETLKVIEAVKRSAVTARRVTLS
>Mature_345_residues
MVDHVKLAVLGAGLIGKRHIRHVLEEPAADLVAVVDPTSVGQKIAEEAGVRWFPSFADMIAVQRPDGVIIATPNQVHVSN
GMEAIEAGIPALIEKPVADDIADGEKLIETAKAKGVPLLTGHHRRHNPMMQKAKQIIESGRLGRILVVNAMFWLFKPDDY
FDMPWRRERGAGPVFLNLIHDIDNLRYLFGDIAAVEARESNVMRGNAVEETAVILIEFKNGILGTASVSDSVVAPWSWEM
TTGENAAYPKTEEACYMIGGTHGSLAVPSLDIWCNPAERSWLEPFDRKRISVETEDPLVLQIRQFCKVIRGHEEPLVSGR
EGLETLKVIEAVKRSAVTARRVTLS

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=192, Percent_Identity=26.5625, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787574, Length=191, Percent_Identity=29.3193717277487, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 38043; Mature: 38043

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHVKLAVLGAGLIGKRHIRHVLEEPAADLVAVVDPTSVGQKIAEEAGVRWFPSFADMI
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEECCCHHHHH
AVQRPDGVIIATPNQVHVSNGMEAIEAGIPALIEKPVADDIADGEKLIETAKAKGVPLLT
EEECCCCEEEECCCEEEECCCHHHHHCCCHHHHHCCCHHHHCCHHHHHHHHHHCCCCEEE
GHHRRHNPMMQKAKQIIESGRLGRILVVNAMFWLFKPDDYFDMPWRRERGAGPVFLNLIH
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHEEECCCCCCCCCCCCCCCCCCCHHHHHH
DIDNLRYLFGDIAAVEARESNVMRGNAVEETAVILIEFKNGILGTASVSDSVVAPWSWEM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCEECCCEEEE
TTGENAAYPKTEEACYMIGGTHGSLAVPSLDIWCNPAERSWLEPFDRKRISVETEDPLVL
CCCCCCCCCCCCCCEEEEECCCCCEECCCEEEEECCCHHHHCCHHHCCEEEECCCCCHHH
QIRQFCKVIRGHEEPLVSGREGLETLKVIEAVKRSAVTARRVTLS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MVDHVKLAVLGAGLIGKRHIRHVLEEPAADLVAVVDPTSVGQKIAEEAGVRWFPSFADMI
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCEECCCHHHHH
AVQRPDGVIIATPNQVHVSNGMEAIEAGIPALIEKPVADDIADGEKLIETAKAKGVPLLT
EEECCCCEEEECCCEEEECCCHHHHHCCCHHHHHCCCHHHHCCHHHHHHHHHHCCCCEEE
GHHRRHNPMMQKAKQIIESGRLGRILVVNAMFWLFKPDDYFDMPWRRERGAGPVFLNLIH
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHEEECCCCCCCCCCCCCCCCCCCHHHHHH
DIDNLRYLFGDIAAVEARESNVMRGNAVEETAVILIEFKNGILGTASVSDSVVAPWSWEM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCEECCCEEEE
TTGENAAYPKTEEACYMIGGTHGSLAVPSLDIWCNPAERSWLEPFDRKRISVETEDPLVL
CCCCCCCCCCCCCCEEEEECCCCCEECCCEEEEECCCHHHHCCHHHCCEEEECCCCCHHH
QIRQFCKVIRGHEEPLVSGREGLETLKVIEAVKRSAVTARRVTLS
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]