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The map label for this gene is hpcG [H]

Identifier: 209551033

GI number: 209551033

Start: 3573278

End: 3574060

Strand: Reverse

Name: hpcG [H]

Synonym: Rleg2_3457

Alternate gene names: 209551033

Gene position: 3574060-3573278 (Counterclockwise)

Preceding gene: 209551034

Following gene: 209551032

Centisome position: 78.76

GC content: 58.11

Gene sequence:

>783_bases
ATGATCACGGACAAGGAACGCCAGGAGGCAGCTGACGCGCTGCTGAAGGCCGAAGTCGAACGAAAGCCAATCGTCCAACC
AAGCAAGACATATCCGAACCTGGAACTCGAAGACGCCTACAAAATACAAGCGCTCTGGGCGCAGGCGCGGATTGCGAAAG
GTGCACGGATCGCTGGCCACAAGATCGGACTGACGTCGCGGGCAATGCAGATAGCCTCGAAAATGACGGAGCCCGATTAC
GGCGTCATCCTCGACGACGCTCTCTTCAATGATGGAGCGCAGATCAAAGCGGATCTGTTCATCAAGCCCCGTCTTGAAGT
CGAACTCGCATTCGTCATGGGCGAGGATCTCGAAGGTCCAAGCGCCCGCATCTATGACGTCATGCGGGCGACCGAATTTG
TCGTTCCCGCTCTCGAAATCATCGACTATCGAACCGAAGTGCCAAGAGCCATCACCGACACAATCGCCGACAACGCGGCT
TTCGGAGCGATCGTCATAGGTGGTCGCATCGTTCGACCGATGGATGTCGATATCCGCTGGGTCGGCGCGACGCTTTCGAA
AAACGGCATCATCGAGGAATCCGGCGTTTCCGCAGCCATCATGGGCCATCCAGCCGCCGGAATTGCATGGCTCGTGAACA
AGCTCCATGCTGTCGGAGGCGGCTTGAAGAAGGGCCAGATCGTTCTTGCCGGCTCGTTCACCCGCCCCGTGGATATCGCC
AAAGGCGACGTCATCCAGGCCGATTATGGGCCGGTCGGCTCCATCGGCGTTTCGTTCGTGTGA

Upstream 100 bases:

>100_bases
AGGGCCGTGGTTTTCGCTCGATTACACATATGTCATGGAGCCGGGCGAAGCCGCCCTGCCACGCCCTCCGATCAAATAAC
GAAAGCAGCAGAGCCAAATC

Downstream 100 bases:

>100_bases
GGTGGCGATGGAACTCCCTGTCAATCACTTTAAACGAAAGCTCATGGCCGGACAAAGCCAGATCGGTCTATGGTGCGGCC
TTCCGGGAAGCTACCCAGCG

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: OHED hydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MITDKERQEAADALLKAEVERKPIVQPSKTYPNLELEDAYKIQALWAQARIAKGARIAGHKIGLTSRAMQIASKMTEPDY
GVILDDALFNDGAQIKADLFIKPRLEVELAFVMGEDLEGPSARIYDVMRATEFVVPALEIIDYRTEVPRAITDTIADNAA
FGAIVIGGRIVRPMDVDIRWVGATLSKNGIIEESGVSAAIMGHPAAGIAWLVNKLHAVGGGLKKGQIVLAGSFTRPVDIA
KGDVIQADYGPVGSIGVSFV

Sequences:

>Translated_260_residues
MITDKERQEAADALLKAEVERKPIVQPSKTYPNLELEDAYKIQALWAQARIAKGARIAGHKIGLTSRAMQIASKMTEPDY
GVILDDALFNDGAQIKADLFIKPRLEVELAFVMGEDLEGPSARIYDVMRATEFVVPALEIIDYRTEVPRAITDTIADNAA
FGAIVIGGRIVRPMDVDIRWVGATLSKNGIIEESGVSAAIMGHPAAGIAWLVNKLHAVGGGLKKGQIVLAGSFTRPVDIA
KGDVIQADYGPVGSIGVSFV
>Mature_260_residues
MITDKERQEAADALLKAEVERKPIVQPSKTYPNLELEDAYKIQALWAQARIAKGARIAGHKIGLTSRAMQIASKMTEPDY
GVILDDALFNDGAQIKADLFIKPRLEVELAFVMGEDLEGPSARIYDVMRATEFVVPALEIIDYRTEVPRAITDTIADNAA
FGAIVIGGRIVRPMDVDIRWVGATLSKNGIIEESGVSAAIMGHPAAGIAWLVNKLHAVGGGLKKGQIVLAGSFTRPVDIA
KGDVIQADYGPVGSIGVSFV

Specific function: Transforms 2-oxo-hept-3-ene-1,7-dioate (OHED) into 2,4- dihydoxy-hept-2-ene-1,7-dioic acid (HHED) [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=224, Percent_Identity=32.5892857142857, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012690 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.-

Molecular weight: Translated: 27936; Mature: 27936

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDKERQEAADALLKAEVERKPIVQPSKTYPNLELEDAYKIQALWAQARIAKGARIAGH
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
KIGLTSRAMQIASKMTEPDYGVILDDALFNDGAQIKADLFIKPRLEVELAFVMGEDLEGP
CCCHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCCC
SARIYDVMRATEFVVPALEIIDYRTEVPRAITDTIADNAAFGAIVIGGRIVRPMDVDIRW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCEEECCCCCEEEE
VGATLSKNGIIEESGVSAAIMGHPAAGIAWLVNKLHAVGGGLKKGQIVLAGSFTRPVDIA
EEEEECCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
KGDVIQADYGPVGSIGVSFV
CCCEEEECCCCCCCCCEECC
>Mature Secondary Structure
MITDKERQEAADALLKAEVERKPIVQPSKTYPNLELEDAYKIQALWAQARIAKGARIAGH
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
KIGLTSRAMQIASKMTEPDYGVILDDALFNDGAQIKADLFIKPRLEVELAFVMGEDLEGP
CCCHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCCC
SARIYDVMRATEFVVPALEIIDYRTEVPRAITDTIADNAAFGAIVIGGRIVRPMDVDIRW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCEEECCCCCEEEE
VGATLSKNGIIEESGVSAAIMGHPAAGIAWLVNKLHAVGGGLKKGQIVLAGSFTRPVDIA
EEEEECCCCCEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
KGDVIQADYGPVGSIGVSFV
CCCEEEECCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7737515 [H]