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The map label for this gene is hpcH [H]

Identifier: 209551032

GI number: 209551032

Start: 3572506

End: 3573270

Strand: Reverse

Name: hpcH [H]

Synonym: Rleg2_3456

Alternate gene names: 209551032

Gene position: 3573270-3572506 (Counterclockwise)

Preceding gene: 209551033

Following gene: 209551031

Centisome position: 78.74

GC content: 60.52

Gene sequence:

>765_bases
ATGGAACTCCCTGTCAATCACTTTAAACGAAAGCTCATGGCCGGACAAAGCCAGATCGGTCTATGGTGCGGCCTTCCGGG
AAGCTACCCAGCGGAAATCGTCGCGCCATCGGGTTTCGATTGGCTACTGTTCGACACCGAGCATTCTCCCAGTGACGTTG
TCACCGTCCTGCCCCAATTGCAGGCGGTCGCGCCCTACAAGGTTTCGCCCGTCGTACGCCCTGCCTTCAACGACCCCGTG
CTGATCAAGCGGTTCCTTGATATCGGCGTGCAGACGCTGCTCGTTCCTTATGTGCAGAATGAGGAGGAGGCGATGGCCGC
TGTAGCAGCCATACGGTATCCGCCGCGCGGGATTCGTGGTGTCTCCGCCTTGACGCGTGCCACCCGCTTCGGGCGTGTTG
CGAACTACGCGCAGATCGCGGAGCAGGAGATATGCCTGCTTCTTCAAATCGAGACCCGTGAAGCGCTCGATCGTCTCGAG
TCCATCGCCGCTGTCGAAGGGGTGGACGGCATCTTCATCGGGCCGGCTGATCTTGCGGCGAGCTTTGGTCATCCTGGTCA
ACCGGGTCACCCTGAAGTGGTTGCTGCGATCGAAGACGCGATAAGGCGCCTGACGGCGCTTGGAAAGCCGACAGGCATAC
TCACCCCCGACGAGAAGTTCGCAGCCCGCTGCATTTCGCTGGGGACGCTGTTTACCGCAGTCGGTGTAGACGTCGCCCTG
CTTGCAAGAGGATCCGAAGCGCTCGCGGCCAGGTTCGCATCTTAG

Upstream 100 bases:

>100_bases
GGCTCGTTCACCCGCCCCGTGGATATCGCCAAAGGCGACGTCATCCAGGCCGATTATGGGCCGGTCGGCTCCATCGGCGT
TTCGTTCGTGTGAGGTGGCG

Downstream 100 bases:

>100_bases
GGAGTTCGAAGATGCCGACGCGGAAGCAGATTTTGGCTGAGATAGCGCCGGCTGGCGCGATCAGGGCCGCCGTCAACATG
TCAAATTCGGCGTTGGTCCG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MELPVNHFKRKLMAGQSQIGLWCGLPGSYPAEIVAPSGFDWLLFDTEHSPSDVVTVLPQLQAVAPYKVSPVVRPAFNDPV
LIKRFLDIGVQTLLVPYVQNEEEAMAAVAAIRYPPRGIRGVSALTRATRFGRVANYAQIAEQEICLLLQIETREALDRLE
SIAAVEGVDGIFIGPADLAASFGHPGQPGHPEVVAAIEDAIRRLTALGKPTGILTPDEKFAARCISLGTLFTAVGVDVAL
LARGSEALAARFAS

Sequences:

>Translated_254_residues
MELPVNHFKRKLMAGQSQIGLWCGLPGSYPAEIVAPSGFDWLLFDTEHSPSDVVTVLPQLQAVAPYKVSPVVRPAFNDPV
LIKRFLDIGVQTLLVPYVQNEEEAMAAVAAIRYPPRGIRGVSALTRATRFGRVANYAQIAEQEICLLLQIETREALDRLE
SIAAVEGVDGIFIGPADLAASFGHPGQPGHPEVVAAIEDAIRRLTALGKPTGILTPDEKFAARCISLGTLFTAVGVDVAL
LARGSEALAARFAS
>Mature_254_residues
MELPVNHFKRKLMAGQSQIGLWCGLPGSYPAEIVAPSGFDWLLFDTEHSPSDVVTVLPQLQAVAPYKVSPVVRPAFNDPV
LIKRFLDIGVQTLLVPYVQNEEEAMAAVAAIRYPPRGIRGVSALTRATRFGRVANYAQIAEQEICLLLQIETREALDRLE
SIAAVEGVDGIFIGPADLAASFGHPGQPGHPEVVAAIEDAIRRLTALGKPTGILTPDEKFAARCISLGTLFTAVGVDVAL
LARGSEALAARFAS

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=249, Percent_Identity=50.6024096385542, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=47.7732793522267, Blast_Score=241, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: NA

Molecular weight: Translated: 27192; Mature: 27192

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPVNHFKRKLMAGQSQIGLWCGLPGSYPAEIVAPSGFDWLLFDTEHSPSDVVTVLPQL
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHH
QAVAPYKVSPVVRPAFNDPVLIKRFLDIGVQTLLVPYVQNEEEAMAAVAAIRYPPRGIRG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHH
VSALTRATRFGRVANYAQIAEQEICLLLQIETREALDRLESIAAVEGVDGIFIGPADLAA
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCCEEEECHHHHHH
SFGHPGQPGHPEVVAAIEDAIRRLTALGKPTGILTPDEKFAARCISLGTLFTAVGVDVAL
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LARGSEALAARFAS
HHCCCHHHHHHCCC
>Mature Secondary Structure
MELPVNHFKRKLMAGQSQIGLWCGLPGSYPAEIVAPSGFDWLLFDTEHSPSDVVTVLPQL
CCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHH
QAVAPYKVSPVVRPAFNDPVLIKRFLDIGVQTLLVPYVQNEEEAMAAVAAIRYPPRGIRG
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHH
VSALTRATRFGRVANYAQIAEQEICLLLQIETREALDRLESIAAVEGVDGIFIGPADLAA
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCCEEEECHHHHHH
SFGHPGQPGHPEVVAAIEDAIRRLTALGKPTGILTPDEKFAARCISLGTLFTAVGVDVAL
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LARGSEALAARFAS
HHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA