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The map label for this gene is mtlK [H]

Identifier: 209551022

GI number: 209551022

Start: 3560965

End: 3562446

Strand: Reverse

Name: mtlK [H]

Synonym: Rleg2_3446

Alternate gene names: 209551022

Gene position: 3562446-3560965 (Counterclockwise)

Preceding gene: 209551023

Following gene: 209551021

Centisome position: 78.5

GC content: 61.4

Gene sequence:

>1482_bases
ATGACGTGCAAACTATCGCTGGCAACGCTTTCCCTAGCGGCACGCACCGCCGCCGTCCCTGGATATGACAGGGCGTCGCT
GAAAGCCGGCATCGTGCACTTCGGCGTCGGCAATTTCCACCGGGCCCATCAGGCGGTCTATCTCGACGACCTCTTCAACG
CAGGCGCAGATCACGACTGGGCGATCGTCGGCGCCGGTGTGCTGCCGTCGGATGCCGCCATGCGCGAAAAGCTTGCGGCA
CAGGATTTTCTGACGACGGTGGTCGAGCAGGACAACAACAAGACGGCGGCGCGCGTCACCGCGCCGATGATCGATATCCT
GCCGATCGGCGATCCCGCCGCGATCATCGCCAAGCTCGCCGATCCCGAAATCCGTGTCGTCTCGATGACGATCACCGAGG
GTGGTTATTTCATCGACGCATCGGGCACATTCAATCCGGCCCATCCGGCGATCGCTGCCGACGGAAACAGCCCCGACGCG
CCGAAGACGGTGTTCGGCCTGATTGTCGCCGGCCTGAAGGCACGCAAGGCGAGGGGTGTCGGTCCTTTCACCGTCATGTC
CTGCGACAACATCCCCCATAACGGCATCGTCACCGCCAATGCCGTGGTCGGCACGGCGGCCCTTTCGGATCCCGGCTTTG
CCGAGTGGATCCGGGAAAACGTCGCCTTCCCGAATGCGATGGTCGACCGCATCACGCCGGCAACCGGCCAGCGCGAGATC
GATTTCCTCAAGGACAATTTCGAGATCGAGGACAATTGGCCGGTCTATTGCGAGGAATTCAAGCAGTGGGTGCTCGAGGA
CAAGTTCACCGCCGGCCGGCCGGCGTTGGAAAAGGTCGGCGTCACCTTCGTGCCCGATGTCACGCCCTATGAGCATATGA
AGATCCGCATCCTCAACGGCGGACATGCGGCGATCGCCTATCCGGCGGCGTTGATGGACATCCATTTCGTTCATGATTCC
ATGGAGGATCCGTTGATTCGCGCCTTCCTCGCCAAGCTCGAGAAAGACGAGATCATCCCGATCGTGCCGCCGGTGCCGAA
CACGTCGCTGACGGATTATTTCGCGCTGATCGAACATCGGCTGCTCAATCCGAAGATTGCCGACACCATTCCGCGGCTGG
CTCAGGACGGCTCCAACCGCCAGCCGAAATTCATCCTGCCGTCGACGCTCGACAATCTCAGCCAGGGCCGGGATGTCGTC
GGCCTCGCGTTGGTCTCGGCGCTCTGGTGCCGTTATTTCGCCGGCAAGACCGACAGCGGCAAGGATATCGTCTTCAACGA
CGCCAGCGCCGAGCGGCTGCATGCAGCAGCGCTGAAGGCAAAGGACGAACCGTCGGCCTTTCTCGCCTTCGACGATATTT
TCGGCGAGGTGGCGAAATCCGAACTTTTCCGCAAGCGTTTCGCCCATGCTCTCAAAACTCTGTGGGAAAAGGGCACACGG
GAGACGCTGCAGCTCTATCTCGACGGCAAGCTCGCAGTGTAG

Upstream 100 bases:

>100_bases
GGTGGTCTTGCCATCTGAGGCAGCTGCGGGCTGGGGGCAGGCATGCGAGCCGGGCGATGGCGCCCAGCCGTTTCGGCAAG
ACCTTCAAGAGGACTGAAAC

Downstream 100 bases:

>100_bases
TGAATCCGGATGGCGGCATTCATTGCCGCCGTCCATCCGGCCTTGCGAGGAAGCGGGACGGTCGTGCGTCCGAAATTGAA
CTCAGGTTGGGTCCTCCATG

Product: Mannitol dehydrogenase domain

Products: NA

Alternate protein names: M2DH; MDH [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTCKLSLATLSLAARTAAVPGYDRASLKAGIVHFGVGNFHRAHQAVYLDDLFNAGADHDWAIVGAGVLPSDAAMREKLAA
QDFLTTVVEQDNNKTAARVTAPMIDILPIGDPAAIIAKLADPEIRVVSMTITEGGYFIDASGTFNPAHPAIAADGNSPDA
PKTVFGLIVAGLKARKARGVGPFTVMSCDNIPHNGIVTANAVVGTAALSDPGFAEWIRENVAFPNAMVDRITPATGQREI
DFLKDNFEIEDNWPVYCEEFKQWVLEDKFTAGRPALEKVGVTFVPDVTPYEHMKIRILNGGHAAIAYPAALMDIHFVHDS
MEDPLIRAFLAKLEKDEIIPIVPPVPNTSLTDYFALIEHRLLNPKIADTIPRLAQDGSNRQPKFILPSTLDNLSQGRDVV
GLALVSALWCRYFAGKTDSGKDIVFNDASAERLHAAALKAKDEPSAFLAFDDIFGEVAKSELFRKRFAHALKTLWEKGTR
ETLQLYLDGKLAV

Sequences:

>Translated_493_residues
MTCKLSLATLSLAARTAAVPGYDRASLKAGIVHFGVGNFHRAHQAVYLDDLFNAGADHDWAIVGAGVLPSDAAMREKLAA
QDFLTTVVEQDNNKTAARVTAPMIDILPIGDPAAIIAKLADPEIRVVSMTITEGGYFIDASGTFNPAHPAIAADGNSPDA
PKTVFGLIVAGLKARKARGVGPFTVMSCDNIPHNGIVTANAVVGTAALSDPGFAEWIRENVAFPNAMVDRITPATGQREI
DFLKDNFEIEDNWPVYCEEFKQWVLEDKFTAGRPALEKVGVTFVPDVTPYEHMKIRILNGGHAAIAYPAALMDIHFVHDS
MEDPLIRAFLAKLEKDEIIPIVPPVPNTSLTDYFALIEHRLLNPKIADTIPRLAQDGSNRQPKFILPSTLDNLSQGRDVV
GLALVSALWCRYFAGKTDSGKDIVFNDASAERLHAAALKAKDEPSAFLAFDDIFGEVAKSELFRKRFAHALKTLWEKGTR
ETLQLYLDGKLAV
>Mature_492_residues
TCKLSLATLSLAARTAAVPGYDRASLKAGIVHFGVGNFHRAHQAVYLDDLFNAGADHDWAIVGAGVLPSDAAMREKLAAQ
DFLTTVVEQDNNKTAARVTAPMIDILPIGDPAAIIAKLADPEIRVVSMTITEGGYFIDASGTFNPAHPAIAADGNSPDAP
KTVFGLIVAGLKARKARGVGPFTVMSCDNIPHNGIVTANAVVGTAALSDPGFAEWIRENVAFPNAMVDRITPATGQREID
FLKDNFEIEDNWPVYCEEFKQWVLEDKFTAGRPALEKVGVTFVPDVTPYEHMKIRILNGGHAAIAYPAALMDIHFVHDSM
EDPLIRAFLAKLEKDEIIPIVPPVPNTSLTDYFALIEHRLLNPKIADTIPRLAQDGSNRQPKFILPSTLDNLSQGRDVVG
LALVSALWCRYFAGKTDSGKDIVFNDASAERLHAAALKAKDEPSAFLAFDDIFGEVAKSELFRKRFAHALKTLWEKGTRE
TLQLYLDGKLAV

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1788497, Length=479, Percent_Identity=36.9519832985386, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790779, Length=474, Percent_Identity=36.4978902953587, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1787823, Length=485, Percent_Identity=36.701030927835, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI48994885, Length=375, Percent_Identity=27.4666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324401, Length=490, Percent_Identity=38.7755102040816, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320765, Length=490, Percent_Identity=38.7755102040816, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.67 [H]

Molecular weight: Translated: 53552; Mature: 53421

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCKLSLATLSLAARTAAVPGYDRASLKAGIVHFGVGNFHRAHQAVYLDDLFNAGADHDW
CCEEEEHHHHHHHHHHHCCCCCCCHHHHHCEEEECCCCHHHHHHHEEHHHHHCCCCCCCE
AIVGAGVLPSDAAMREKLAAQDFLTTVVEQDNNKTAARVTAPMIDILPIGDPAAIIAKLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCHHHHHHHHC
DPEIRVVSMTITEGGYFIDASGTFNPAHPAIAADGNSPDAPKTVFGLIVAGLKARKARGV
CCCEEEEEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GPFTVMSCDNIPHNGIVTANAVVGTAALSDPGFAEWIRENVAFPNAMVDRITPATGQREI
CCEEEEECCCCCCCCEEEECCEEEEHHCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCH
DFLKDNFEIEDNWPVYCEEFKQWVLEDKFTAGRPALEKVGVTFVPDVTPYEHMKIRILNG
HHHHCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEECCCCCCCCCEEEEEEEC
GHAAIAYPAALMDIHFVHDSMEDPLIRAFLAKLEKDEIIPIVPPVPNTSLTDYFALIEHR
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHH
LLNPKIADTIPRLAQDGSNRQPKFILPSTLDNLSQGRDVVGLALVSALWCRYFAGKTDSG
HCCCHHHHHHHHHHHCCCCCCCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KDIVFNDASAERLHAAALKAKDEPSAFLAFDDIFGEVAKSELFRKRFAHALKTLWEKGTR
CEEEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETLQLYLDGKLAV
EEEEEEECCEECC
>Mature Secondary Structure 
TCKLSLATLSLAARTAAVPGYDRASLKAGIVHFGVGNFHRAHQAVYLDDLFNAGADHDW
CEEEEHHHHHHHHHHHCCCCCCCHHHHHCEEEECCCCHHHHHHHEEHHHHHCCCCCCCE
AIVGAGVLPSDAAMREKLAAQDFLTTVVEQDNNKTAARVTAPMIDILPIGDPAAIIAKLA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCHHHHHHHHC
DPEIRVVSMTITEGGYFIDASGTFNPAHPAIAADGNSPDAPKTVFGLIVAGLKARKARGV
CCCEEEEEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GPFTVMSCDNIPHNGIVTANAVVGTAALSDPGFAEWIRENVAFPNAMVDRITPATGQREI
CCEEEEECCCCCCCCEEEECCEEEEHHCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCH
DFLKDNFEIEDNWPVYCEEFKQWVLEDKFTAGRPALEKVGVTFVPDVTPYEHMKIRILNG
HHHHCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEECCCCCCCCCEEEEEEEC
GHAAIAYPAALMDIHFVHDSMEDPLIRAFLAKLEKDEIIPIVPPVPNTSLTDYFALIEHR
CCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHH
LLNPKIADTIPRLAQDGSNRQPKFILPSTLDNLSQGRDVVGLALVSALWCRYFAGKTDSG
HCCCHHHHHHHHHHHCCCCCCCCEECCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KDIVFNDASAERLHAAALKAKDEPSAFLAFDDIFGEVAKSELFRKRFAHALKTLWEKGTR
CEEEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETLQLYLDGKLAV
EEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8254318 [H]