Click here to switch to the map view.

The map label for this gene is yieH [H]

Identifier: 209551021

GI number: 209551021

Start: 3560178

End: 3560867

Strand: Reverse

Name: yieH [H]

Synonym: Rleg2_3445

Alternate gene names: 209551021

Gene position: 3560867-3560178 (Counterclockwise)

Preceding gene: 209551022

Following gene: 209551020

Centisome position: 78.47

GC content: 61.45

Gene sequence:

>690_bases
ATGGCTGATGCTGAACCGCAGCTGATCATCTTCGATTGCGACGGCGTGCTCGTCGACAGCGAGCCGATCTCGATCAGCGT
GCTGGTCGCCGCGATGAACGATCTCGGCGTCTCGATCACCGAGGACCAAGCCTATGAGCGCTTTCTCGGCCGCAGCCTGT
CGACGATGGTCGACACGCTGGACACCGAATTCAACGTTCATGCCGGCGAGGAATTTCTCGAGCGCATCCGCACCGATCTC
TACGCCCGTTTTCGCACCGAATTGAAGCCGATCGACGGTATCGCCGCAGCGATCGATGGGCTCGGCGTTCGCTGCTGCGT
CGCCTCCTCAAGCCAGATGGAGCGGATCCGGCTGTCGCTGTCCGTCACCGGGCTGCTCGGCAGGCTGCCCGACATCTTCA
GTGCGACGATGGTCAAGCGCGGCAAACCGGCGCCCGATCTTTTCCTGCATGCAGCGCGTGAAATGCAGGTAGAGCCTGCG
CACTGCATTGTCGTCGAGGACAGCCCGGCCGGCATTGCCGCCGCCAAGGCGGCGGGTATGACGGTGTTTGCCTTCACCGG
CGGATCGCATGCCAACTTCGCCGGTTATCGCGCCGAACTCGACCGGCTTTCGCCTGATGTGGTGTTTGACGCCATGCCTG
AATTGATACACCTTGTCCGCAACCATAAGCTGGACGGGATCAAGATTTGA

Upstream 100 bases:

>100_bases
TAGTGAATCCGGATGGCGGCATTCATTGCCGCCGTCCATCCGGCCTTGCGAGGAAGCGGGACGGTCGTGCGTCCGAAATT
GAACTCAGGTTGGGTCCTCC

Downstream 100 bases:

>100_bases
TGCGGGATCATGTGGTTGCGGTGGATGTCGGCACCGGCAGTGCGCGCGCCGGCGTCTTCGATGCCCGTGGCCGCCTGCTC
GCCAAGGCCGAACATCCGAT

Product: HAD-superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MADAEPQLIIFDCDGVLVDSEPISISVLVAAMNDLGVSITEDQAYERFLGRSLSTMVDTLDTEFNVHAGEEFLERIRTDL
YARFRTELKPIDGIAAAIDGLGVRCCVASSSQMERIRLSLSVTGLLGRLPDIFSATMVKRGKPAPDLFLHAAREMQVEPA
HCIVVEDSPAGIAAAKAAGMTVFAFTGGSHANFAGYRAELDRLSPDVVFDAMPELIHLVRNHKLDGIKI

Sequences:

>Translated_229_residues
MADAEPQLIIFDCDGVLVDSEPISISVLVAAMNDLGVSITEDQAYERFLGRSLSTMVDTLDTEFNVHAGEEFLERIRTDL
YARFRTELKPIDGIAAAIDGLGVRCCVASSSQMERIRLSLSVTGLLGRLPDIFSATMVKRGKPAPDLFLHAAREMQVEPA
HCIVVEDSPAGIAAAKAAGMTVFAFTGGSHANFAGYRAELDRLSPDVVFDAMPELIHLVRNHKLDGIKI
>Mature_228_residues
ADAEPQLIIFDCDGVLVDSEPISISVLVAAMNDLGVSITEDQAYERFLGRSLSTMVDTLDTEFNVHAGEEFLERIRTDLY
ARFRTELKPIDGIAAAIDGLGVRCCVASSSQMERIRLSLSVTGLLGRLPDIFSATMVKRGKPAPDLFLHAAREMQVEPAH
CIVVEDSPAGIAAAKAAGMTVFAFTGGSHANFAGYRAELDRLSPDVVFDAMPELIHLVRNHKLDGIKI

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=186, Percent_Identity=32.7956989247312, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788021, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789046, Length=178, Percent_Identity=29.7752808988764, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24819; Mature: 24688

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAEPQLIIFDCDGVLVDSEPISISVLVAAMNDLGVSITEDQAYERFLGRSLSTMVDTL
CCCCCCCEEEEECCCEEECCCCCEEEEEEEEHHHCCCEEEHHHHHHHHHCCHHHHHHHHH
DTEFNVHAGEEFLERIRTDLYARFRTELKPIDGIAAAIDGLGVRCCVASSSQMERIRLSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHHHEEEEE
SVTGLLGRLPDIFSATMVKRGKPAPDLFLHAAREMQVEPAHCIVVEDSPAGIAAAKAAGM
EHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCE
TVFAFTGGSHANFAGYRAELDRLSPDVVFDAMPELIHLVRNHKLDGIKI
EEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADAEPQLIIFDCDGVLVDSEPISISVLVAAMNDLGVSITEDQAYERFLGRSLSTMVDTL
CCCCCCEEEEECCCEEECCCCCEEEEEEEEHHHCCCEEEHHHHHHHHHCCHHHHHHHHH
DTEFNVHAGEEFLERIRTDLYARFRTELKPIDGIAAAIDGLGVRCCVASSSQMERIRLSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHHHEEEEE
SVTGLLGRLPDIFSATMVKRGKPAPDLFLHAAREMQVEPAHCIVVEDSPAGIAAAKAAGM
EHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCE
TVFAFTGGSHANFAGYRAELDRLSPDVVFDAMPELIHLVRNHKLDGIKI
EEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]