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The map label for this gene is glgX [H]

Identifier: 209550928

GI number: 209550928

Start: 3459617

End: 3461596

Strand: Direct

Name: glgX [H]

Synonym: Rleg2_3352

Alternate gene names: 209550928

Gene position: 3459617-3461596 (Clockwise)

Preceding gene: 209550927

Following gene: 209550929

Centisome position: 76.24

GC content: 63.48

Gene sequence:

>1980_bases
ATGCGGGGAGAGAGTTCGGCGCAAAGCGGCGCGATCGTCTTCGAGACCGGCGTCGAATTTGCCGTGTGGTCGCATCACGC
CGCACAGATCGAACTCTGCCTCTTCGAGGATGACGGCAACAGGGAATTCGCGCGCCTGCCGATGGCGCGCGACAGCAATC
ACTTCCACCGTCTGTTTGTCGACGGACTGAAGGCCGGCGCCCGCTACGGCTATCGCGCCGACGGCATCTACGCCCCTGAC
AACGGCCTCTGGTATGATCCCTCCAAACTGCTGATCGATCCCTACGCCAAGGAGATCGATAGGCCGTTCCGCTACGATCC
CCGCCTCGGCATCTATGGCGAGGACAGCCAGGATCTGATGCCGAAGGCGATCGTCACCACCGACACGCATGCCGAAATCG
GCAAGCCGCTCTTCCAGCCGGGCGGCTTCATTTACGAAGTGGCTGTCCGGCCCTTCACCATCCTCCATCCCGACGTGCCG
GAGGCTCAGCGCGGCACGATCGCCGCCCTTGCCCATCCCTCCGTCATCGCGCATCTGAAGCGCATCGGTGTCGACGCCGT
CGAACTGATGCCGATCACCGCCTGGATCGACGAGCGCCACCTGCCGCCGCTCGGCCTCACCAACGGCTGGGGCTACAATC
CGGTCGCCTTCATGGCGCTCGATCCGCGGCTGGCGCCGGGCGGCATGGCGGAGCTGCGCCAGACGGTCGCCGCCCTCCAT
GACGAAGGCATCGCCGTCATCCTCGATCTTGTCTTCAATCATACCGGCGAGAGCGACCGTTACGGGACGACGCTGTCGCT
GCGCGGCCTCGACAACCTGCATTATTATCGCCACGCCCGGAATATGCCGGGCGAACTTGTCAACGACACCGGCACCGGCA
ACACGCTCGCCTGCGATCATCCCGAGGTGCGCCGTCTCGTCATCGACAGCCTGCGCCATTTCGTGCTCAATTCAGGCATC
GACGGTTTTCGCTTCGATCTCGCCCCGGTGCTCGGCCGCACCGAGACGGGCTTCGAGCGCGACGGCGGAACGCTTGCCGC
GATCCTCGCCGATAATGTGCTTGCCGACCGGATCATGATTGCCGAACCTTGGGATATCGGCCCGGGCGGTTATCAGCTCG
GCAATTTCCCGCCGTCTTTCCTCGAATGGAACGACCGGCTTCGTGACGATCTGCGCTGCTACTGGCGCGGCGACGATTGG
AAGACCGGGGCGCTCGCCACCGCACTCGCCGGCTCCTCCGACATCTTCTCCCGCAACGACGGCAAAGAGACGCGCAGCGT
CAATTTCCTCGCTGCCCATGACGGTTTCACGCTGACCGATCTCGTCTCCTATGCCGGCAAGCACAATGACGCCAATGGCG
AGCACAATCGTGACGGCCATAACGAGAACCACTCCTGGAACAACGGCGTCGAGGGGGAGACGGTCTATCCCTCGATCCGC
AAGCGTCGCCGTGAGGATGTGATGGCGCTGATATCGACACTCTTTGCCACCCGCGGCAGCATCATGCTGACCGCGGGCGA
CGAGGGCGGCCGCAGTCAGCATGGCAACAACAACGCCTATTGCCAGGACAATGAGATCACCTGGCTGGACTGGAAGGCGC
TGAACGAGGATCTCATCGCCCATACAGCCTTCGTCGCCGGGCTGCGCCGCCGCTTCACGGTCTTTTCCGAAACAGGCTTC
CTGGCGGGCAACGGCGATGTCGAATGGATTTCGCTCTCCGGCGAGCCGATGACCGTTTCCGAATGGGAGACACCGTCGCT
TTCCACCCTCGGCATGCTGCTTTCCACAGGTGACCGCTCCGCGCGCGGCAGGCAGACCCGGCTTGCCGTGCTCTTCAACC
GTTCGGGCGACCGCCAGTTTTTCACGCTCCCGTCCGAAATCGAACCGGGCTGGCGTCAATTGACACCGGATGGAGCCAGG
AAAACCGCCGCCCGCGTCACCGTCGAGCCACGCTCCGTCGCCTTTTTCGTAGAAAAGTGA

Upstream 100 bases:

>100_bases
AGCGCTCGCCGATCTGATCGCCGCCGCCGAAAGCATTGCCAGCATTCGTGAACGCACGGGACGCGACGCGCCAACCGTGA
TCACCTGATCCGGGGGGAAC

Downstream 100 bases:

>100_bases
CCGCCTCCCCTCGCTTGCGCAAATCCTTGTCGCAATCGTCATAAATGCACGATAAGGCTTTGGCTGGCGGCCCATCTCAC
GAGGAATTCATGGTCACGGA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MRGESSAQSGAIVFETGVEFAVWSHHAAQIELCLFEDDGNREFARLPMARDSNHFHRLFVDGLKAGARYGYRADGIYAPD
NGLWYDPSKLLIDPYAKEIDRPFRYDPRLGIYGEDSQDLMPKAIVTTDTHAEIGKPLFQPGGFIYEVAVRPFTILHPDVP
EAQRGTIAALAHPSVIAHLKRIGVDAVELMPITAWIDERHLPPLGLTNGWGYNPVAFMALDPRLAPGGMAELRQTVAALH
DEGIAVILDLVFNHTGESDRYGTTLSLRGLDNLHYYRHARNMPGELVNDTGTGNTLACDHPEVRRLVIDSLRHFVLNSGI
DGFRFDLAPVLGRTETGFERDGGTLAAILADNVLADRIMIAEPWDIGPGGYQLGNFPPSFLEWNDRLRDDLRCYWRGDDW
KTGALATALAGSSDIFSRNDGKETRSVNFLAAHDGFTLTDLVSYAGKHNDANGEHNRDGHNENHSWNNGVEGETVYPSIR
KRRREDVMALISTLFATRGSIMLTAGDEGGRSQHGNNNAYCQDNEITWLDWKALNEDLIAHTAFVAGLRRRFTVFSETGF
LAGNGDVEWISLSGEPMTVSEWETPSLSTLGMLLSTGDRSARGRQTRLAVLFNRSGDRQFFTLPSEIEPGWRQLTPDGAR
KTAARVTVEPRSVAFFVEK

Sequences:

>Translated_659_residues
MRGESSAQSGAIVFETGVEFAVWSHHAAQIELCLFEDDGNREFARLPMARDSNHFHRLFVDGLKAGARYGYRADGIYAPD
NGLWYDPSKLLIDPYAKEIDRPFRYDPRLGIYGEDSQDLMPKAIVTTDTHAEIGKPLFQPGGFIYEVAVRPFTILHPDVP
EAQRGTIAALAHPSVIAHLKRIGVDAVELMPITAWIDERHLPPLGLTNGWGYNPVAFMALDPRLAPGGMAELRQTVAALH
DEGIAVILDLVFNHTGESDRYGTTLSLRGLDNLHYYRHARNMPGELVNDTGTGNTLACDHPEVRRLVIDSLRHFVLNSGI
DGFRFDLAPVLGRTETGFERDGGTLAAILADNVLADRIMIAEPWDIGPGGYQLGNFPPSFLEWNDRLRDDLRCYWRGDDW
KTGALATALAGSSDIFSRNDGKETRSVNFLAAHDGFTLTDLVSYAGKHNDANGEHNRDGHNENHSWNNGVEGETVYPSIR
KRRREDVMALISTLFATRGSIMLTAGDEGGRSQHGNNNAYCQDNEITWLDWKALNEDLIAHTAFVAGLRRRFTVFSETGF
LAGNGDVEWISLSGEPMTVSEWETPSLSTLGMLLSTGDRSARGRQTRLAVLFNRSGDRQFFTLPSEIEPGWRQLTPDGAR
KTAARVTVEPRSVAFFVEK
>Mature_659_residues
MRGESSAQSGAIVFETGVEFAVWSHHAAQIELCLFEDDGNREFARLPMARDSNHFHRLFVDGLKAGARYGYRADGIYAPD
NGLWYDPSKLLIDPYAKEIDRPFRYDPRLGIYGEDSQDLMPKAIVTTDTHAEIGKPLFQPGGFIYEVAVRPFTILHPDVP
EAQRGTIAALAHPSVIAHLKRIGVDAVELMPITAWIDERHLPPLGLTNGWGYNPVAFMALDPRLAPGGMAELRQTVAALH
DEGIAVILDLVFNHTGESDRYGTTLSLRGLDNLHYYRHARNMPGELVNDTGTGNTLACDHPEVRRLVIDSLRHFVLNSGI
DGFRFDLAPVLGRTETGFERDGGTLAAILADNVLADRIMIAEPWDIGPGGYQLGNFPPSFLEWNDRLRDDLRCYWRGDDW
KTGALATALAGSSDIFSRNDGKETRSVNFLAAHDGFTLTDLVSYAGKHNDANGEHNRDGHNENHSWNNGVEGETVYPSIR
KRRREDVMALISTLFATRGSIMLTAGDEGGRSQHGNNNAYCQDNEITWLDWKALNEDLIAHTAFVAGLRRRFTVFSETGF
LAGNGDVEWISLSGEPMTVSEWETPSLSTLGMLLSTGDRSARGRQTRLAVLFNRSGDRQFFTLPSEIEPGWRQLTPDGAR
KTAARVTVEPRSVAFFVEK

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=601, Percent_Identity=44.0931780366057, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73136; Mature: 73136

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGESSAQSGAIVFETGVEFAVWSHHAAQIELCLFEDDGNREFARLPMARDSNHFHRLFV
CCCCCCCCCCEEEEECCCCEEEECCCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHH
DGLKAGARYGYRADGIYAPDNGLWYDPSKLLIDPYAKEIDRPFRYDPRLGIYGEDSQDLM
HHHHHCCCCCCCCCCEECCCCCCEECCCHHEECHHHHHCCCCCCCCCCCCCCCCCCHHCC
PKAIVTTDTHAEIGKPLFQPGGFIYEVAVRPFTILHPDVPEAQRGTIAALAHPSVIAHLK
CCEEEECCCHHHHCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCEEEECCHHHHHHHH
RIGVDAVELMPITAWIDERHLPPLGLTNGWGYNPVAFMALDPRLAPGGMAELRQTVAALH
HCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
DEGIAVILDLVFNHTGESDRYGTTLSLRGLDNLHYYRHARNMPGELVNDTGTGNTLACDH
CCCHHEEEEHHHCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCHHCCCCCCCCEEECCC
PEVRRLVIDSLRHFVLNSGIDGFRFDLAPVLGRTETGFERDGGTLAAILADNVLADRIMI
HHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCEEEE
AEPWDIGPGGYQLGNFPPSFLEWNDRLRDDLRCYWRGDDWKTGALATALAGSSDIFSRND
ECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCC
GKETRSVNFLAAHDGFTLTDLVSYAGKHNDANGEHNRDGHNENHSWNNGVEGETVYPSIR
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHH
KRRREDVMALISTLFATRGSIMLTAGDEGGRSQHGNNNAYCQDNEITWLDWKALNEDLIA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEHHHCCHHHHH
HTAFVAGLRRRFTVFSETGFLAGNGDVEWISLSGEPMTVSEWETPSLSTLGMLLSTGDRS
HHHHHHHHHHHEEEEECCCEEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCC
ARGRQTRLAVLFNRSGDRQFFTLPSEIEPGWRQLTPDGARKTAARVTVEPRSVAFFVEK
CCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEEEECCCEEEEEEEC
>Mature Secondary Structure
MRGESSAQSGAIVFETGVEFAVWSHHAAQIELCLFEDDGNREFARLPMARDSNHFHRLFV
CCCCCCCCCCEEEEECCCCEEEECCCCEEEEEEEEECCCCCEEEECCCCCCCCCHHHHHH
DGLKAGARYGYRADGIYAPDNGLWYDPSKLLIDPYAKEIDRPFRYDPRLGIYGEDSQDLM
HHHHHCCCCCCCCCCEECCCCCCEECCCHHEECHHHHHCCCCCCCCCCCCCCCCCCHHCC
PKAIVTTDTHAEIGKPLFQPGGFIYEVAVRPFTILHPDVPEAQRGTIAALAHPSVIAHLK
CCEEEECCCHHHHCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCEEEECCHHHHHHHH
RIGVDAVELMPITAWIDERHLPPLGLTNGWGYNPVAFMALDPRLAPGGMAELRQTVAALH
HCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
DEGIAVILDLVFNHTGESDRYGTTLSLRGLDNLHYYRHARNMPGELVNDTGTGNTLACDH
CCCHHEEEEHHHCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCHHCCCCCCCCEEECCC
PEVRRLVIDSLRHFVLNSGIDGFRFDLAPVLGRTETGFERDGGTLAAILADNVLADRIMI
HHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCEEEE
AEPWDIGPGGYQLGNFPPSFLEWNDRLRDDLRCYWRGDDWKTGALATALAGSSDIFSRND
ECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCCCCC
GKETRSVNFLAAHDGFTLTDLVSYAGKHNDANGEHNRDGHNENHSWNNGVEGETVYPSIR
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHH
KRRREDVMALISTLFATRGSIMLTAGDEGGRSQHGNNNAYCQDNEITWLDWKALNEDLIA
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEHHHCCHHHHH
HTAFVAGLRRRFTVFSETGFLAGNGDVEWISLSGEPMTVSEWETPSLSTLGMLLSTGDRS
HHHHHHHHHHHEEEEECCCEEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHCCCCC
ARGRQTRLAVLFNRSGDRQFFTLPSEIEPGWRQLTPDGARKTAARVTVEPRSVAFFVEK
CCCCCEEEEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA