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The map label for this gene is pldB [H]

Identifier: 209550898

GI number: 209550898

Start: 3420614

End: 3421588

Strand: Direct

Name: pldB [H]

Synonym: Rleg2_3322

Alternate gene names: 209550898

Gene position: 3420614-3421588 (Clockwise)

Preceding gene: 209550896

Following gene: 209550901

Centisome position: 75.38

GC content: 63.79

Gene sequence:

>975_bases
ATGGATCAGGTTCTCTATTCGACGCCCGACAATCCCGCCCCGGAAAACCGCACGGAAGGGTTCTTCGAAACCCATGACGG
CCACCGGCTGCGGTATGCCGTTTTTCGCGCCAGTGCCCAGGTCGCCAAGGGCACGGTCGTCATCCTGCACGGCCGCAACG
AATATATCGAGAAATATTTCGAGACGATCCGCGACCTGACGGCCAAAGGTCTCTGGGTCGCAACCTTCGATATGCGCGGC
CAGGGCGGTTCGGGGCGGCTGCTGAAGCGCCGCAACCACGGCCACATCCGCCGCTTCGGCGATTACGAACGTGATCTCGA
CACCTTCCTCGAAAAGGTGGTGCTGCCGGATACCCGCCTGCCCTTCTACCTGCTCGCCCATTCCACAGGCGGGCTGATCG
CGCTCTCGGCCGCGCCCTATCTCAACACCCGTATCGATCGCATGGTGCTGTCGGCGCCCTTTATCGGCCTGACCGGCCAG
TCGGCCTCACCCCGCGTCATCCGCGCCTTGGCCGGCACGTTGACGGCAATCGGTCTTGGTTCCTTGCCGCTGACCTCGAA
ACTGAAGGAACCGGATTTCCGCGACAACCCGCTGACCTCGGACGAAACCCGCTTCGAGCGTAATGTCGCGATGATGAAGG
CCCATCCGGAGCTGACGCTCGGGCCGCCAACGGCCCGCTGGCTGACCGAGGCTTTCCGGACGATGGACCGGGTCACCTCG
CCCTACCATCTCTTTTCGATCACGATCCCGACCATCGTCATCGCTCCGACGCGTGACGGTGTCGTGCCTTACACCGCGCA
GGAGCGGCTCTCGCGTTATTTCCGCGCCGGTCAACTGGTGCCGATCAACGGCGCCCGCCACGAGATCTTCCAGGAACGCG
ATACCTACCGCGCCGCCGCCCTTGCCGCCTTCCACGCCTTCATTCCCGGCAGCGATGCCGAAGAGAACCACGACGTCGCC
GCCCTAGGAACCTGA

Upstream 100 bases:

>100_bases
CCTTTTCGCGCTTTGCAGCCTTGCGGACGCCGCTTTCCAAGCCGGATGCCATGCGTTATCCCTGATGCGAAGCAGACAGC
AGCGGCGCATTGGCAAAGTC

Downstream 100 bases:

>100_bases
CACCCGATCGGCACGGCCGACCTTGGGATCAACGCTGCAGAACGGCGATCGCCTGCTCATAGACGGCCCGGCTGCCGGCG
GCGATGATCGAGCCGCCGTT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDQVLYSTPDNPAPENRTEGFFETHDGHRLRYAVFRASAQVAKGTVVILHGRNEYIEKYFETIRDLTAKGLWVATFDMRG
QGGSGRLLKRRNHGHIRRFGDYERDLDTFLEKVVLPDTRLPFYLLAHSTGGLIALSAAPYLNTRIDRMVLSAPFIGLTGQ
SASPRVIRALAGTLTAIGLGSLPLTSKLKEPDFRDNPLTSDETRFERNVAMMKAHPELTLGPPTARWLTEAFRTMDRVTS
PYHLFSITIPTIVIAPTRDGVVPYTAQERLSRYFRAGQLVPINGARHEIFQERDTYRAAALAAFHAFIPGSDAEENHDVA
ALGT

Sequences:

>Translated_324_residues
MDQVLYSTPDNPAPENRTEGFFETHDGHRLRYAVFRASAQVAKGTVVILHGRNEYIEKYFETIRDLTAKGLWVATFDMRG
QGGSGRLLKRRNHGHIRRFGDYERDLDTFLEKVVLPDTRLPFYLLAHSTGGLIALSAAPYLNTRIDRMVLSAPFIGLTGQ
SASPRVIRALAGTLTAIGLGSLPLTSKLKEPDFRDNPLTSDETRFERNVAMMKAHPELTLGPPTARWLTEAFRTMDRVTS
PYHLFSITIPTIVIAPTRDGVVPYTAQERLSRYFRAGQLVPINGARHEIFQERDTYRAAALAAFHAFIPGSDAEENHDVA
ALGT
>Mature_324_residues
MDQVLYSTPDNPAPENRTEGFFETHDGHRLRYAVFRASAQVAKGTVVILHGRNEYIEKYFETIRDLTAKGLWVATFDMRG
QGGSGRLLKRRNHGHIRRFGDYERDLDTFLEKVVLPDTRLPFYLLAHSTGGLIALSAAPYLNTRIDRMVLSAPFIGLTGQ
SASPRVIRALAGTLTAIGLGSLPLTSKLKEPDFRDNPLTSDETRFERNVAMMKAHPELTLGPPTARWLTEAFRTMDRVTS
PYHLFSITIPTIVIAPTRDGVVPYTAQERLSRYFRAGQLVPINGARHEIFQERDTYRAAALAAFHAFIPGSDAEENHDVA
ALGT

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=302, Percent_Identity=31.4569536423841, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 36195; Mature: 36195

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQVLYSTPDNPAPENRTEGFFETHDGHRLRYAVFRASAQVAKGTVVILHGRNEYIEKYF
CCCCEECCCCCCCCCCCCCCCEECCCCCEEEEEEEHHHHHHCCCEEEEEECCHHHHHHHH
ETIRDLTAKGLWVATFDMRGQGGSGRLLKRRNHGHIRRFGDYERDLDTFLEKVVLPDTRL
HHHHHHHHCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PFYLLAHSTGGLIALSAAPYLNTRIDRMVLSAPFIGLTGQSASPRVIRALAGTLTAIGLG
CEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCC
SLPLTSKLKEPDFRDNPLTSDETRFERNVAMMKAHPELTLGPPTARWLTEAFRTMDRVTS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEECCCHHHHHHHHHHHHHHCCC
PYHLFSITIPTIVIAPTRDGVVPYTAQERLSRYFRAGQLVPINGARHEIFQERDTYRAAA
CEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHH
LAAFHAFIPGSDAEENHDVAALGT
HHHHHHHCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDQVLYSTPDNPAPENRTEGFFETHDGHRLRYAVFRASAQVAKGTVVILHGRNEYIEKYF
CCCCEECCCCCCCCCCCCCCCEECCCCCEEEEEEEHHHHHHCCCEEEEEECCHHHHHHHH
ETIRDLTAKGLWVATFDMRGQGGSGRLLKRRNHGHIRRFGDYERDLDTFLEKVVLPDTRL
HHHHHHHHCCEEEEEEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PFYLLAHSTGGLIALSAAPYLNTRIDRMVLSAPFIGLTGQSASPRVIRALAGTLTAIGLG
CEEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCC
SLPLTSKLKEPDFRDNPLTSDETRFERNVAMMKAHPELTLGPPTARWLTEAFRTMDRVTS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEECCCCEEECCCHHHHHHHHHHHHHHCCC
PYHLFSITIPTIVIAPTRDGVVPYTAQERLSRYFRAGQLVPINGARHEIFQERDTYRAAA
CEEEEEEEECEEEEECCCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHH
LAAFHAFIPGSDAEENHDVAALGT
HHHHHHHCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]