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The map label for this gene is hcaE [H]

Identifier: 209550888

GI number: 209550888

Start: 3404519

End: 3405745

Strand: Direct

Name: hcaE [H]

Synonym: Rleg2_3312

Alternate gene names: 209550888

Gene position: 3404519-3405745 (Clockwise)

Preceding gene: 209550887

Following gene: 209550890

Centisome position: 75.02

GC content: 58.68

Gene sequence:

>1227_bases
ATGTCGGTATTTGCAACTGCTGCGCCATCGGTCGACTGCATCGATCAGCTCATTGCTGCTCACAAGCCGGGCCACGGTCT
TGCCCGGCCATTCTACATGGATCCGGACATTTATGAGCGTGATCTGGAGCGCGTGTTCCGCCGCCATTGGCATTGCCTCG
CGCATGAGAGCGTTGTTCCAAAGCCGAATGACTTCGAGGTGTTTCGGATGGCCTCAGAACAGGTGATCCTCACGCGCCAT
GCCGATGGAACAATTCATGCGCTGCTCAATGTCTGCCGCCATCGCGGCGCCGAGGTCTGTACCCGGGATAAGGGCAATGC
CCGTGTCTTCGTCTGTCCCTACCATGCCTGGACCTATGGCAATGACGGAAGCCTGAAGGCGGCAAGACTGATGCCAAAGG
ATTTCAAGCGGGAGGATTATGGGCTGAAGACGCTGCATGTCCGTGTGGTTGAAGGCTTGATCTTCATTTCCTTCGCAGAC
GAGCCGCTCGATTTCTCCGAGGTCGAGAATCTGCTCCATGCGACCTGCGGCCAATATGGCTGGGGGCAGGCTAAGGTGGC
GCATCGGCAGACCTATCCGGTCGACGCCAACTGGAAACTGGCGGTGGAAAACTATGTGGAATGCTATCATTGCGGCCCGG
CGCACCCCGAATATTCGCAGACTCACGCGCTGGAACAGCCGGCGCACATGATTGAGGCGCTGAACGCCTCGATGGAGGAA
CGCACACGCGCCCTCGGCATCGAGGTCGGTTCCGGCGATCGCTGGCAGACATCTGCGAAAAGCAGAGAAGCCATCCACGC
GTTCCGCTATGCGCTTTATGACGGGGTGAAGACCGGCAGCCAAGACGGCTCGCCGCTCAGCATGCTGATGGGCCGATTTA
CGGAATTTGATGGCGGCGTCACCTCCGTCCATCTGGGCGGCACCTCATTTCTCGTCTGCTACCCCGACCACGGTATGATC
TATCGCTTCATTCCGAAGGGCCCGAGAGCTTGCGAGATGGAACTGATCTGGCTCGTCAACAGTGAAGCCGAGGAGGGCAG
AGACTACGATCTCGCCAAGCTCGTGTGGCTCTGGACGGTGACGACTGACGAGGACAAGGCCATCATCGAGCACACGTCGC
GCGGCGTTGCCTCGCACTATTTTTTGCCTGGACCGATCGCGCCGATGGAGCAGAACGAGCTGCGCTATATCAACTGGTAT
CTGGACGAGATTGGTCGGCCGAACTGA

Upstream 100 bases:

>100_bases
TTTACGAAATCGAGATTGCGGGAACGAGGGTGAGAGCCGAGCCGTCACTTGCGCCGATGTATGACCCGAATGCCGCGAGG
GTTCGGATGTGACAACGGAC

Downstream 100 bases:

>100_bases
CCGAGTTCCAATGCATCTTGCCCGCAAGTGTTCAGCGGTTCCGGGATAACAACATGCAAAGCGCCGCGCATCTGGGAGGA
TACGCGGCGCTCTAACATTT

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSVFATAAPSVDCIDQLIAAHKPGHGLARPFYMDPDIYERDLERVFRRHWHCLAHESVVPKPNDFEVFRMASEQVILTRH
ADGTIHALLNVCRHRGAEVCTRDKGNARVFVCPYHAWTYGNDGSLKAARLMPKDFKREDYGLKTLHVRVVEGLIFISFAD
EPLDFSEVENLLHATCGQYGWGQAKVAHRQTYPVDANWKLAVENYVECYHCGPAHPEYSQTHALEQPAHMIEALNASMEE
RTRALGIEVGSGDRWQTSAKSREAIHAFRYALYDGVKTGSQDGSPLSMLMGRFTEFDGGVTSVHLGGTSFLVCYPDHGMI
YRFIPKGPRACEMELIWLVNSEAEEGRDYDLAKLVWLWTVTTDEDKAIIEHTSRGVASHYFLPGPIAPMEQNELRYINWY
LDEIGRPN

Sequences:

>Translated_408_residues
MSVFATAAPSVDCIDQLIAAHKPGHGLARPFYMDPDIYERDLERVFRRHWHCLAHESVVPKPNDFEVFRMASEQVILTRH
ADGTIHALLNVCRHRGAEVCTRDKGNARVFVCPYHAWTYGNDGSLKAARLMPKDFKREDYGLKTLHVRVVEGLIFISFAD
EPLDFSEVENLLHATCGQYGWGQAKVAHRQTYPVDANWKLAVENYVECYHCGPAHPEYSQTHALEQPAHMIEALNASMEE
RTRALGIEVGSGDRWQTSAKSREAIHAFRYALYDGVKTGSQDGSPLSMLMGRFTEFDGGVTSVHLGGTSFLVCYPDHGMI
YRFIPKGPRACEMELIWLVNSEAEEGRDYDLAKLVWLWTVTTDEDKAIIEHTSRGVASHYFLPGPIAPMEQNELRYINWY
LDEIGRPN
>Mature_407_residues
SVFATAAPSVDCIDQLIAAHKPGHGLARPFYMDPDIYERDLERVFRRHWHCLAHESVVPKPNDFEVFRMASEQVILTRHA
DGTIHALLNVCRHRGAEVCTRDKGNARVFVCPYHAWTYGNDGSLKAARLMPKDFKREDYGLKTLHVRVVEGLIFISFADE
PLDFSEVENLLHATCGQYGWGQAKVAHRQTYPVDANWKLAVENYVECYHCGPAHPEYSQTHALEQPAHMIEALNASMEER
TRALGIEVGSGDRWQTSAKSREAIHAFRYALYDGVKTGSQDGSPLSMLMGRFTEFDGGVTSVHLGGTSFLVCYPDHGMIY
RFIPKGPRACEMELIWLVNSEAEEGRDYDLAKLVWLWTVTTDEDKAIIEHTSRGVASHYFLPGPIAPMEQNELRYINWYL
DEIGRPN

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=260, Percent_Identity=33.8461538461538, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788103, Length=208, Percent_Identity=33.1730769230769, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 46245; Mature: 46114

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFATAAPSVDCIDQLIAAHKPGHGLARPFYMDPDIYERDLERVFRRHWHCLAHESVVP
CCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KPNDFEVFRMASEQVILTRHADGTIHALLNVCRHRGAEVCTRDKGNARVFVCPYHAWTYG
CCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEEECEEEEEC
NDGSLKAARLMPKDFKREDYGLKTLHVRVVEGLIFISFADEPLDFSEVENLLHATCGQYG
CCCCCCHHHCCCHHHCCCCCCCCEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHCCCC
WGQAKVAHRQTYPVDANWKLAVENYVECYHCGPAHPEYSQTHALEQPAHMIEALNASMEE
CCCCEECCCCCCCCCCCCEEEHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
RTRALGIEVGSGDRWQTSAKSREAIHAFRYALYDGVKTGSQDGSPLSMLMGRFTEFDGGV
HHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCE
TSVHLGGTSFLVCYPDHGMIYRFIPKGPRACEMELIWLVNSEAEEGRDYDLAKLVWLWTV
EEEEECCEEEEEEECCCCEEEEECCCCCCCEEEEEEEEECCCHHCCCCCCCEEEEEEEEE
TTDEDKAIIEHTSRGVASHYFLPGPIAPMEQNELRYINWYLDEIGRPN
ECCCCHHHHHHHCCCCHHEEECCCCCCCCCCCCEEEEEEEHHHCCCCC
>Mature Secondary Structure 
SVFATAAPSVDCIDQLIAAHKPGHGLARPFYMDPDIYERDLERVFRRHWHCLAHESVVP
CEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KPNDFEVFRMASEQVILTRHADGTIHALLNVCRHRGAEVCTRDKGNARVFVCPYHAWTYG
CCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCEEEEEEECEEEEEC
NDGSLKAARLMPKDFKREDYGLKTLHVRVVEGLIFISFADEPLDFSEVENLLHATCGQYG
CCCCCCHHHCCCHHHCCCCCCCCEEEEEEECEEEEEEECCCCCCHHHHHHHHHHHHCCCC
WGQAKVAHRQTYPVDANWKLAVENYVECYHCGPAHPEYSQTHALEQPAHMIEALNASMEE
CCCCEECCCCCCCCCCCCEEEHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
RTRALGIEVGSGDRWQTSAKSREAIHAFRYALYDGVKTGSQDGSPLSMLMGRFTEFDGGV
HHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCE
TSVHLGGTSFLVCYPDHGMIYRFIPKGPRACEMELIWLVNSEAEEGRDYDLAKLVWLWTV
EEEEECCEEEEEEECCCCEEEEECCCCCCCEEEEEEEEECCCHHCCCCCCCEEEEEEEEE
TTDEDKAIIEHTSRGVASHYFLPGPIAPMEQNELRYINWYLDEIGRPN
ECCCCHHHHHHHCCCCHHEEECCCCCCCCCCCCEEEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]